| Definition | Bacillus cereus B4264, complete genome. |
|---|---|
| Accession | NC_011725 |
| Length | 5,419,036 |
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The map label for this gene is dhaK [H]
Identifier: 218233925
GI number: 218233925
Start: 988412
End: 990163
Strand: Direct
Name: dhaK [H]
Synonym: BCB4264_A1008
Alternate gene names: 218233925
Gene position: 988412-990163 (Clockwise)
Preceding gene: 218231524
Following gene: 218231587
Centisome position: 18.24
GC content: 42.18
Gene sequence:
>1752_bases ATGAAAAAGATTATAAACAAACCAGAAACATTAGTAATGGAAATGTGCAACGGAATGGTTATGGCTCATCCAGAGCTTGA GCTTTTGAAAAAATATAAAGTCATCAAGAAGAAAGAAATGAACGAAAACAAAGTAACGTTAATTAGTGGCGGCGGTAGTG GTCATGAGCCGGCACATGCAGGATTAGTCGGAAAAGGAATGTTAGATGCGGCAGTGTGCGGAGATGTGTTTGCGTCACCT TCACAAATTCAGGTATACCAAGCGATTAAAGAGACAGCTAGTAAAAAAGGTACGTTATTAATTATTAAAAATTATAGCGG CGATATTATGAATTTCAAAAACGGTGCGCATTTAGCGACGGAAGATGGAATTGAAGTCGACTACGTAAAGGTGGACGATG ATATTGCGGTAGAAGATAGCCTGTACACAGTAGGGCGCCGCGGCGTTGCGGGCGTTATTTTAGTTCATAAAATTGCTGGC GCAGCAGCGGAAGAAGGTATGGATTTAGGAGCGGTGAAAGCTGTAGCAGAAAAAGCAGCGGCTAACGTGCGCACAATCGG TTTAGCATTAACGTCTTGTACAGTTCCAGCGAGCGGATCACCTACTTTCACACTTGCGGAAGATGAAATGGAATACGGCG TAGGTATTCACGGCGAACCAGGAATTAAGCGTGAAAAAATGCTGTCAGCAGATGAACTAGCGAACCGTATGACAAATGAT CTTGTAAAAGATTTAGGAGTAAAAGATGGAGAAGAAATTGCGCTTCTAGTTAACGGTTTTGGCGGAACACCACTGCAAGA ACTTTACTTATTTAACAACGCAGTTACGAGAGAACTAGCTGCAAGAAACATTAAAATTAATAGAGTATTCGTCGGTAACT ATATGACAAGTATTGATATGGCTGGTATGTCTTTAACGGTAATGAAGTTAGATGAAGAGCTAAAAACATTACTATCAAAA GAATGTAATACACCAGCGTTTAAAGTAGACGGTCCAGTTGAAAGTGTAGAGTACGTAAATGTACTTGAAGAGACAGAAGA GAAGGAAGTTTCATTTGAACTAGAAACGGCGGAAGAACACGCTGTTATTAATGATAATGTTATTACATTAAACAACATGA TTTATCTCGTTGATAAAATGAGCGACGTTATTATTAAAAATGAAGTACCGTTCTGTGAGCTAGATACGCATGCTGGTGAT GGCGACTTCGGAATGAGTGTGGCAAAAGGATTTAAGCAGTTAAAACGTGAGTGGCATTCGATTGTAGAACAAGAGAACGT AACGATCGGATCATTCCTTGACGGTTGTTCCATGATTATTATGGAACATTGCGGCGGCGCATCTGGTCCAATTTGGGGCG GTGCATTCCGCGCAGCTAGTAAAGCAGCAGGCGAAAAACGTGAGCTAACAGTGAAAGAATTCGCTGAAATACTGCAAGCA GCATTGCAAGGCATCCAATCGATCGGTGAAAGATCATTCGGCAGAGGAGCAGTAGTTGGTGACAAAACACTTGTTGACGC ACTTGCTCCATGTGTAGATTCTTGGTTAGATAGCGCCTCAAACGAAGTAGACGTAAAAACTGCCTTTGAAAAAGGAGCAG AAGCAGCGGTCAAAGGAGCAGAGTACACGAAAGAAATCGTAGCTCGCATGGGCCGCGCTGGTACAGTTGGCGAAAGAAGC TTAGGATATCCAGATGCAGGTGCACATGCACTTGGGGTTATCTTTACGGAGATTGCGGGTAGTTTGAAATAG
Upstream 100 bases:
>100_bases TTTGTCCGTTTACTATAAAACGCTTTCATTTTAACATGGTTAGTAAGAAGAAGATTTCAAATATATCACAATATATTAAA GGAATTGAGGGAGAACTACA
Downstream 100 bases:
>100_bases GAATTGGAAAGTCCCTCTGCCTTAAGGTGGAGGGATTTTTATATAGTTGATATGTAGAAGACTTCTTTCGGTTCTTTCTC AGTAAAATATGAGTTTGTTA
Product: dihydroxyacetone kinase
Products: ADP; glycerone phosphate
Alternate protein names: NA
Number of amino acids: Translated: 583; Mature: 583
Protein sequence:
>583_residues MKKIINKPETLVMEMCNGMVMAHPELELLKKYKVIKKKEMNENKVTLISGGGSGHEPAHAGLVGKGMLDAAVCGDVFASP SQIQVYQAIKETASKKGTLLIIKNYSGDIMNFKNGAHLATEDGIEVDYVKVDDDIAVEDSLYTVGRRGVAGVILVHKIAG AAAEEGMDLGAVKAVAEKAAANVRTIGLALTSCTVPASGSPTFTLAEDEMEYGVGIHGEPGIKREKMLSADELANRMTND LVKDLGVKDGEEIALLVNGFGGTPLQELYLFNNAVTRELAARNIKINRVFVGNYMTSIDMAGMSLTVMKLDEELKTLLSK ECNTPAFKVDGPVESVEYVNVLEETEEKEVSFELETAEEHAVINDNVITLNNMIYLVDKMSDVIIKNEVPFCELDTHAGD GDFGMSVAKGFKQLKREWHSIVEQENVTIGSFLDGCSMIIMEHCGGASGPIWGGAFRAASKAAGEKRELTVKEFAEILQA ALQGIQSIGERSFGRGAVVGDKTLVDALAPCVDSWLDSASNEVDVKTAFEKGAEAAVKGAEYTKEIVARMGRAGTVGERS LGYPDAGAHALGVIFTEIAGSLK
Sequences:
>Translated_583_residues MKKIINKPETLVMEMCNGMVMAHPELELLKKYKVIKKKEMNENKVTLISGGGSGHEPAHAGLVGKGMLDAAVCGDVFASP SQIQVYQAIKETASKKGTLLIIKNYSGDIMNFKNGAHLATEDGIEVDYVKVDDDIAVEDSLYTVGRRGVAGVILVHKIAG AAAEEGMDLGAVKAVAEKAAANVRTIGLALTSCTVPASGSPTFTLAEDEMEYGVGIHGEPGIKREKMLSADELANRMTND LVKDLGVKDGEEIALLVNGFGGTPLQELYLFNNAVTRELAARNIKINRVFVGNYMTSIDMAGMSLTVMKLDEELKTLLSK ECNTPAFKVDGPVESVEYVNVLEETEEKEVSFELETAEEHAVINDNVITLNNMIYLVDKMSDVIIKNEVPFCELDTHAGD GDFGMSVAKGFKQLKREWHSIVEQENVTIGSFLDGCSMIIMEHCGGASGPIWGGAFRAASKAAGEKRELTVKEFAEILQA ALQGIQSIGERSFGRGAVVGDKTLVDALAPCVDSWLDSASNEVDVKTAFEKGAEAAVKGAEYTKEIVARMGRAGTVGERS LGYPDAGAHALGVIFTEIAGSLK >Mature_583_residues MKKIINKPETLVMEMCNGMVMAHPELELLKKYKVIKKKEMNENKVTLISGGGSGHEPAHAGLVGKGMLDAAVCGDVFASP SQIQVYQAIKETASKKGTLLIIKNYSGDIMNFKNGAHLATEDGIEVDYVKVDDDIAVEDSLYTVGRRGVAGVILVHKIAG AAAEEGMDLGAVKAVAEKAAANVRTIGLALTSCTVPASGSPTFTLAEDEMEYGVGIHGEPGIKREKMLSADELANRMTND LVKDLGVKDGEEIALLVNGFGGTPLQELYLFNNAVTRELAARNIKINRVFVGNYMTSIDMAGMSLTVMKLDEELKTLLSK ECNTPAFKVDGPVESVEYVNVLEETEEKEVSFELETAEEHAVINDNVITLNNMIYLVDKMSDVIIKNEVPFCELDTHAGD GDFGMSVAKGFKQLKREWHSIVEQENVTIGSFLDGCSMIIMEHCGGASGPIWGGAFRAASKAAGEKRELTVKEFAEILQA ALQGIQSIGERSFGRGAVVGDKTLVDALAPCVDSWLDSASNEVDVKTAFEKGAEAAVKGAEYTKEIVARMGRAGTVGERS LGYPDAGAHALGVIFTEIAGSLK
Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone [H]
COG id: COG2376
COG function: function code G; Dihydroxyacetone kinase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 DhaK domain [H]
Homologues:
Organism=Homo sapiens, GI20149621, Length=579, Percent_Identity=33.3333333333333, Blast_Score=285, Evalue=6e-77, Organism=Escherichia coli, GI87081857, Length=353, Percent_Identity=40.5099150141643, Blast_Score=275, Evalue=6e-75, Organism=Escherichia coli, GI1787449, Length=212, Percent_Identity=28.3018867924528, Blast_Score=91, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17565018, Length=581, Percent_Identity=31.1531841652324, Blast_Score=266, Evalue=2e-71, Organism=Saccharomyces cerevisiae, GI6321055, Length=603, Percent_Identity=31.1774461028192, Blast_Score=222, Evalue=1e-58, Organism=Saccharomyces cerevisiae, GI6323570, Length=591, Percent_Identity=32.6565143824027, Blast_Score=209, Evalue=8e-55,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004006 - InterPro: IPR012736 [H]
Pfam domain/function: PF02733 Dak1 [H]
EC number: 2.7.1.29
Molecular weight: Translated: 62452; Mature: 62452
Theoretical pI: Translated: 4.68; Mature: 4.68
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 3.8 %Met (Translated Protein) 5.1 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 3.8 %Met (Mature Protein) 5.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKIINKPETLVMEMCNGMVMAHPELELLKKYKVIKKKEMNENKVTLISGGGSGHEPAHA CCCCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC GLVGKGMLDAAVCGDVFASPSQIQVYQAIKETASKKGTLLIIKNYSGDIMNFKNGAHLAT CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCEEC EDGIEVDYVKVDDDIAVEDSLYTVGRRGVAGVILVHKIAGAAAEEGMDLGAVKAVAEKAA CCCCEEEEEEECCCCEECCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH ANVRTIGLALTSCTVPASGSPTFTLAEDEMEYGVGIHGEPGIKREKMLSADELANRMTND CCHHEEEEEEEEEECCCCCCCCEEEECCCHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHH LVKDLGVKDGEEIALLVNGFGGTPLQELYLFNNAVTRELAARNIKINRVFVGNYMTSIDM HHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHHH AGMSLTVMKLDEELKTLLSKECNTPAFKVDGPVESVEYVNVLEETEEKEVSFELETAEEH CCCEEEEEHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCHHHEEEEEECCCCC AVINDNVITLNNMIYLVDKMSDVIIKNEVPFCELDTHAGDGDFGMSVAKGFKQLKREWHS EEECCCEEEECCEEEEEEHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH IVEQENVTIGSFLDGCSMIIMEHCGGASGPIWGGAFRAASKAAGEKRELTVKEFAEILQA HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH ALQGIQSIGERSFGRGAVVGDKTLVDALAPCVDSWLDSASNEVDVKTAFEKGAEAAVKGA HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHHHHH EYTKEIVARMGRAGTVGERSLGYPDAGAHALGVIFTEIAGSLK HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC >Mature Secondary Structure MKKIINKPETLVMEMCNGMVMAHPELELLKKYKVIKKKEMNENKVTLISGGGSGHEPAHA CCCCCCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC GLVGKGMLDAAVCGDVFASPSQIQVYQAIKETASKKGTLLIIKNYSGDIMNFKNGAHLAT CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCEEC EDGIEVDYVKVDDDIAVEDSLYTVGRRGVAGVILVHKIAGAAAEEGMDLGAVKAVAEKAA CCCCEEEEEEECCCCEECCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH ANVRTIGLALTSCTVPASGSPTFTLAEDEMEYGVGIHGEPGIKREKMLSADELANRMTND CCHHEEEEEEEEEECCCCCCCCEEEECCCHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHH LVKDLGVKDGEEIALLVNGFGGTPLQELYLFNNAVTRELAARNIKINRVFVGNYMTSIDM HHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCHHHHHHH AGMSLTVMKLDEELKTLLSKECNTPAFKVDGPVESVEYVNVLEETEEKEVSFELETAEEH CCCEEEEEHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCHHHEEEEEECCCCC AVINDNVITLNNMIYLVDKMSDVIIKNEVPFCELDTHAGDGDFGMSVAKGFKQLKREWHS EEECCCEEEECCEEEEEEHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH IVEQENVTIGSFLDGCSMIIMEHCGGASGPIWGGAFRAASKAAGEKRELTVKEFAEILQA HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH ALQGIQSIGERSFGRGAVVGDKTLVDALAPCVDSWLDSASNEVDVKTAFEKGAEAAVKGA HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHHHHH EYTKEIVARMGRAGTVGERSLGYPDAGAHALGVIFTEIAGSLK HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: ATP; glycerone
Specific reaction: ATP + glycerone = ADP + glycerone phosphate
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11337471 [H]