| Definition | Bacillus cereus B4264, complete genome. |
|---|---|
| Accession | NC_011725 |
| Length | 5,419,036 |
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The map label for this gene is pps [H]
Identifier: 218233787
GI number: 218233787
Start: 2978088
End: 2980694
Strand: Reverse
Name: pps [H]
Synonym: BCB4264_A3108
Alternate gene names: 218233787
Gene position: 2980694-2978088 (Counterclockwise)
Preceding gene: 218234340
Following gene: 218231061
Centisome position: 55.0
GC content: 38.51
Gene sequence:
>2607_bases ATGAGTTCTTTCGTTCTCGATTTTCAGGAAATAGAAAAAACGCAGCTTTTTCTCGTTGGTGGAAAAGGATTGAATTTAGG GGAGTTATCCAATATTCAAGGGATACAAGTGCCAGAAGGGTTTTGTGTTACAACGGTAGGGTATGAACAGGCCATCGGAA AAAATGGAGCGTTTCAAACTTTATTGAATCAATTAGCGATGCTAAAAATTGAGGAACGAGATCGAATTGGTGAAATTAGT AAGCAGATTAGAGAAGTCATTATGGCGGTGGAAATTCCAGTTGATGTAGTGGAATCAGTAGCTCATTATCTCTCGCACTT TGGCGATGAACATGCATATGCAGTGCGTTCGAGTGCTACTGCTGAAGATTTACCGTATGCCTCGTTTGCTGGTCAACAAG ATACGTATTTAAATGTTATTGGAAAAGAAAACATTTTGCAGCATATTAAAAAATGCTGGGCTTCTTTGTTTACAGATCGG GCGGTTATATACCGAATGCAAAACGGTTTTGATCATAATCAAGTTTCTATATGTGTTGTCATTCAAAAAATGGTTTTCCC TGAGGCTTCAGGGATTTTATTTACTGCGGATCCGATTACTTCGAATAGGAAGGTGTTGTCAATCGATGCCAGCTTTGGAC TTGGCGAGGCGCTAGTATCTGGATTGGTTTCTACCGATAATTATAAGGTAAAAGAAGACGAAATCGTCGAAAAGGTCATC GCAACGAAAAAATTAGCCATCTATGGACGAAAAGAAGGCGGAACAGAGAGAAAGAAAATTGCTCCTAATCAGCAAAAATT TCAAACACTTACTGAACAACAGATTTTACAACTAGCACGCATCGGAAGACAAATCGAAGCTTATTTTGGTTGTCCGCAAG ATATTGAATGGTGCTTAGTTGATGATACAATTTATATAGTCCAAAGCAGACCGATTACGACTTTATATCCAATTCCAGAA GTAAATGATGGGGAAAATCACGTGTATATATCAGTTGGTCACCAACAAATGATGACCGATGCGATGAAACCATTAGGTTT ATCTTTTTTCCTGTTAACGACTAGTGCACCTATGTGTAAAGCCGGAGGAAGACTATTTGTTGATGCTACGCAAAGACTAG CTTCACCTGCTAGCAGAGATTACTTAATAAACACTTTAGGTAAATCGGATCCGCTCATACGAGATGCATTAACGACTGTA ATTGAGCGAGAGAATTTTATTAAATTGTTATCGGATGATGAAAAAGAAAAGGATCTTAGTAAAAATGTGCCACCTGCAAG TTCGCAACAACAACCTGAAAACGATCCGGAAATCGTTACGAATTTAATAAAGAATAGTGAATTATCAATAGAAGAATTAA AACGAAACATGCAAACGAAATCAGGGGTGGATGTACTTGATTTTATTTTAGAAGACATTCAGCAATTAAAGAAAGTATTA TTTAACTCGCAAAGTATAGCTATCATTATGGCAGGTATGAATGCTTCATCATGGATTAATGAAAAGATGGAGCAGTGGCT AGGAGAAAAAAATGCAGCAGATGTACTTTCACAATCTGTACAACATAATATTACGTCAGAAATGGGGCTAGCGTTATTGG ATGTGGCAGATGTGATTCGGCCGTATCCGGAAGTCATTGCGTATTTACAGCATGTAGAAGATGACAGCTTTTTAGATGAA TTAATTCAGTTTAAAGGCGGGGAAAAAGTTCGGGATGCAATCGATGCTTTTCTAAATAAATATGGAATGAGATGCAGTGG AGAAATCGACATTACGAAAACGCGCTGGAGTGAACAGCCAGCTACAATTATACCGATGATTTTAAATCATATAAGAGACT TTGAATATGGTGCTAGTAAGCGGAAGTTTGAAGAAGGGCTGCAGGAAGCTTTGAAAAAAGAAAAAGAGTTGTTAGAGCGA TTGCAGCACTTGCCGGATGGGGAACAAAAAGTAGAAGAGACGAAGAGAATGATTCGTAACATCCGCAATTTCATCGGTTA TCGTGAATATCCGAAATACGGCATGATTCATCGTTATTTCATATATAAGCAGGCATTACTGAAAGAAGTAGAGAAACTTG TGCAAAATAATGTTCTGGATGAAATAGAAGATATATACTATCTAACTTTTGAAGAACTTCACGAAGTCGTTCGCACAAAT AAACTAGATTACAAGATTATTCATAAACAAAAAAATGCGTACAAATTATATGAAAAACTAACACCTCCGCGTGTTATCAC GTCTGATGGAGAAATCATTACAGGTAAGTACAAACGCGAAAATCTCCCGGCTGAAGCAATTGTAGGTTTGCCTGTTTCTT GTGGAGTGGTTGAGGGGAGAGCACGCGTTATTTTAAACATGGAAGACGCGAATTTGGAAGATGGAGATATATTAGTTACG GCATTTACCGATCCTGGGTGGACACCTTTGTTTGTTTCTATAAAAGGGTTAGTCACTGAAGTCGGCGGGCTTATGACGCA CGGAGCAGTGATTGCACGTGAGTATGGCCTGCCAGCAGTTGTTGGAGTAGAGAATGCTACGAAACTAATAAAAGACGGGC AACGAATTCGGGTACATGGAACAGAAGGATATATTGAAGTGTTGTAG
Upstream 100 bases:
>100_bases AACTTAATTATATCATGTAATTTACGTTCGAAATAGACTGTTTCTTCTCATTTACTCCTGCTACAATCAAGAACACATGA ATAGTAAGGAGAGGTGCCAA
Downstream 100 bases:
>100_bases TTGTATTTTAGATAAATAAAGAAATCGCCCACTAAATATAGTGGACGATTTCTTTATGATTTTAATAGTTTTATCAACTT TCATCAATATTTTCTGAAAT
Product: phosphoenolpyruvate synthase
Products: AMP; phosphoenolpyruvate; phosphate
Alternate protein names: Putative PEP synthase; Pyruvate, water dikinase [H]
Number of amino acids: Translated: 868; Mature: 867
Protein sequence:
>868_residues MSSFVLDFQEIEKTQLFLVGGKGLNLGELSNIQGIQVPEGFCVTTVGYEQAIGKNGAFQTLLNQLAMLKIEERDRIGEIS KQIREVIMAVEIPVDVVESVAHYLSHFGDEHAYAVRSSATAEDLPYASFAGQQDTYLNVIGKENILQHIKKCWASLFTDR AVIYRMQNGFDHNQVSICVVIQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSTDNYKVKEDEIVEKVI ATKKLAIYGRKEGGTERKKIAPNQQKFQTLTEQQILQLARIGRQIEAYFGCPQDIEWCLVDDTIYIVQSRPITTLYPIPE VNDGENHVYISVGHQQMMTDAMKPLGLSFFLLTTSAPMCKAGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTV IERENFIKLLSDDEKEKDLSKNVPPASSQQQPENDPEIVTNLIKNSELSIEELKRNMQTKSGVDVLDFILEDIQQLKKVL FNSQSIAIIMAGMNASSWINEKMEQWLGEKNAADVLSQSVQHNITSEMGLALLDVADVIRPYPEVIAYLQHVEDDSFLDE LIQFKGGEKVRDAIDAFLNKYGMRCSGEIDITKTRWSEQPATIIPMILNHIRDFEYGASKRKFEEGLQEALKKEKELLER LQHLPDGEQKVEETKRMIRNIRNFIGYREYPKYGMIHRYFIYKQALLKEVEKLVQNNVLDEIEDIYYLTFEELHEVVRTN KLDYKIIHKQKNAYKLYEKLTPPRVITSDGEIITGKYKRENLPAEAIVGLPVSCGVVEGRARVILNMEDANLEDGDILVT AFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL
Sequences:
>Translated_868_residues MSSFVLDFQEIEKTQLFLVGGKGLNLGELSNIQGIQVPEGFCVTTVGYEQAIGKNGAFQTLLNQLAMLKIEERDRIGEIS KQIREVIMAVEIPVDVVESVAHYLSHFGDEHAYAVRSSATAEDLPYASFAGQQDTYLNVIGKENILQHIKKCWASLFTDR AVIYRMQNGFDHNQVSICVVIQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSTDNYKVKEDEIVEKVI ATKKLAIYGRKEGGTERKKIAPNQQKFQTLTEQQILQLARIGRQIEAYFGCPQDIEWCLVDDTIYIVQSRPITTLYPIPE VNDGENHVYISVGHQQMMTDAMKPLGLSFFLLTTSAPMCKAGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTV IERENFIKLLSDDEKEKDLSKNVPPASSQQQPENDPEIVTNLIKNSELSIEELKRNMQTKSGVDVLDFILEDIQQLKKVL FNSQSIAIIMAGMNASSWINEKMEQWLGEKNAADVLSQSVQHNITSEMGLALLDVADVIRPYPEVIAYLQHVEDDSFLDE LIQFKGGEKVRDAIDAFLNKYGMRCSGEIDITKTRWSEQPATIIPMILNHIRDFEYGASKRKFEEGLQEALKKEKELLER LQHLPDGEQKVEETKRMIRNIRNFIGYREYPKYGMIHRYFIYKQALLKEVEKLVQNNVLDEIEDIYYLTFEELHEVVRTN KLDYKIIHKQKNAYKLYEKLTPPRVITSDGEIITGKYKRENLPAEAIVGLPVSCGVVEGRARVILNMEDANLEDGDILVT AFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL >Mature_867_residues SSFVLDFQEIEKTQLFLVGGKGLNLGELSNIQGIQVPEGFCVTTVGYEQAIGKNGAFQTLLNQLAMLKIEERDRIGEISK QIREVIMAVEIPVDVVESVAHYLSHFGDEHAYAVRSSATAEDLPYASFAGQQDTYLNVIGKENILQHIKKCWASLFTDRA VIYRMQNGFDHNQVSICVVIQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSTDNYKVKEDEIVEKVIA TKKLAIYGRKEGGTERKKIAPNQQKFQTLTEQQILQLARIGRQIEAYFGCPQDIEWCLVDDTIYIVQSRPITTLYPIPEV NDGENHVYISVGHQQMMTDAMKPLGLSFFLLTTSAPMCKAGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTVI ERENFIKLLSDDEKEKDLSKNVPPASSQQQPENDPEIVTNLIKNSELSIEELKRNMQTKSGVDVLDFILEDIQQLKKVLF NSQSIAIIMAGMNASSWINEKMEQWLGEKNAADVLSQSVQHNITSEMGLALLDVADVIRPYPEVIAYLQHVEDDSFLDEL IQFKGGEKVRDAIDAFLNKYGMRCSGEIDITKTRWSEQPATIIPMILNHIRDFEYGASKRKFEEGLQEALKKEKELLERL QHLPDGEQKVEETKRMIRNIRNFIGYREYPKYGMIHRYFIYKQALLKEVEKLVQNNVLDEIEDIYYLTFEELHEVVRTNK LDYKIIHKQKNAYKLYEKLTPPRVITSDGEIITGKYKRENLPAEAIVGLPVSCGVVEGRARVILNMEDANLEDGDILVTA FTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAVVGVENATKLIKDGQRIRVHGTEGYIEVL
Specific function: Might catalyze the phosphorylation of pyruvate to phosphoenolpyruvate (Potential) [H]
COG id: COG0574
COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PEP-utilizing enzyme family [H]
Homologues:
Organism=Escherichia coli, GI1787994, Length=333, Percent_Identity=34.2342342342342, Blast_Score=177, Evalue=2e-45, Organism=Caenorhabditis elegans, GI17564524, Length=343, Percent_Identity=30.0291545189504, Blast_Score=127, Evalue=3e-29,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR008279 - InterPro: IPR002192 [H]
Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]
EC number: 2.7.9.2
Molecular weight: Translated: 97787; Mature: 97656
Theoretical pI: Translated: 5.02; Mature: 5.02
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSSFVLDFQEIEKTQLFLVGGKGLNLGELSNIQGIQVPEGFCVTTVGYEQAIGKNGAFQT CCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCHHHH LLNQLAMLKIEERDRIGEISKQIREVIMAVEIPVDVVESVAHYLSHFGDEHAYAVRSSAT HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHECCCCC AEDLPYASFAGQQDTYLNVIGKENILQHIKKCWASLFTDRAVIYRMQNGFDHNQVSICVV CCCCCCHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHH IQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSTDNYKVKEDEIVEKVI HHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH ATKKLAIYGRKEGGTERKKIAPNQQKFQTLTEQQILQLARIGRQIEAYFGCPQDIEWCLV HHHHHEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE DDTIYIVQSRPITTLYPIPEVNDGENHVYISVGHQQMMTDAMKPLGLSFFLLTTSAPMCK CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCHHH AGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTVIERENFIKLLSDDEKEKDLS CCCEEEEEHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH KNVPPASSQQQPENDPEIVTNLIKNSELSIEELKRNMQTKSGVDVLDFILEDIQQLKKVL CCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH FNSQSIAIIMAGMNASSWINEKMEQWLGEKNAADVLSQSVQHNITSEMGLALLDVADVIR HCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC PYPEVIAYLQHVEDDSFLDELIQFKGGEKVRDAIDAFLNKYGMRCSGEIDITKTRWSEQP CHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC ATIIPMILNHIRDFEYGASKRKFEEGLQEALKKEKELLERLQHLPDGEQKVEETKRMIRN CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH IRNFIGYREYPKYGMIHRYFIYKQALLKEVEKLVQNNVLDEIEDIYYLTFEELHEVVRTN HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC KLDYKIIHKQKNAYKLYEKLTPPRVITSDGEIITGKYKRENLPAEAIVGLPVSCGVVEGR CCCEEEEECCCHHHHHHHHCCCCEEEECCCCEEECCCCCCCCCHHHHCCCCCCCCEECCC ARVILNMEDANLEDGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAV EEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEE VGVENATKLIKDGQRIRVHGTEGYIEVL EECHHHHHHHHCCCEEEEECCCCEEEEC >Mature Secondary Structure SSFVLDFQEIEKTQLFLVGGKGLNLGELSNIQGIQVPEGFCVTTVGYEQAIGKNGAFQT CCCCHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCHHHH LLNQLAMLKIEERDRIGEISKQIREVIMAVEIPVDVVESVAHYLSHFGDEHAYAVRSSAT HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHECCCCC AEDLPYASFAGQQDTYLNVIGKENILQHIKKCWASLFTDRAVIYRMQNGFDHNQVSICVV CCCCCCHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHH IQKMVFPEASGILFTADPITSNRKVLSIDASFGLGEALVSGLVSTDNYKVKEDEIVEKVI HHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH ATKKLAIYGRKEGGTERKKIAPNQQKFQTLTEQQILQLARIGRQIEAYFGCPQDIEWCLV HHHHHEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE DDTIYIVQSRPITTLYPIPEVNDGENHVYISVGHQQMMTDAMKPLGLSFFLLTTSAPMCK CCEEEEEECCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHCCCEEEEEECCCCHHH AGGRLFVDATQRLASPASRDYLINTLGKSDPLIRDALTTVIERENFIKLLSDDEKEKDLS CCCEEEEEHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH KNVPPASSQQQPENDPEIVTNLIKNSELSIEELKRNMQTKSGVDVLDFILEDIQQLKKVL CCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH FNSQSIAIIMAGMNASSWINEKMEQWLGEKNAADVLSQSVQHNITSEMGLALLDVADVIR HCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC PYPEVIAYLQHVEDDSFLDELIQFKGGEKVRDAIDAFLNKYGMRCSGEIDITKTRWSEQP CHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC ATIIPMILNHIRDFEYGASKRKFEEGLQEALKKEKELLERLQHLPDGEQKVEETKRMIRN CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH IRNFIGYREYPKYGMIHRYFIYKQALLKEVEKLVQNNVLDEIEDIYYLTFEELHEVVRTN HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC KLDYKIIHKQKNAYKLYEKLTPPRVITSDGEIITGKYKRENLPAEAIVGLPVSCGVVEGR CCCEEEEECCCHHHHHHHHCCCCEEEECCCCEEECCCCCCCCCHHHHCCCCCCCCEECCC ARVILNMEDANLEDGDILVTAFTDPGWTPLFVSIKGLVTEVGGLMTHGAVIAREYGLPAV EEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEE VGVENATKLIKDGQRIRVHGTEGYIEVL EECHHHHHHHHCCCEEEEECCCCEEEEC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: Mg2+; Mn2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.083 {pyruvate}} 10.5 {phosphate}} 0.028 {ATP}} [C]
Substrates: ATP; pyruvate; H2O
Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate
General reaction: Phospho group transfer [C]
Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377; 7704256 [H]