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The map label for this gene is alsS [H]

Identifier: 218233733

GI number: 218233733

Start: 884277

End: 885965

Strand: Direct

Name: alsS [H]

Synonym: BCB4264_A0920

Alternate gene names: 218233733

Gene position: 884277-885965 (Clockwise)

Preceding gene: 218232617

Following gene: 218234205

Centisome position: 16.32

GC content: 40.56

Gene sequence:

>1689_bases
TTGAGTACAGGTGTAAAAGCAAACGACGTGAAGACAAAAACAAAAGGAGCAGATCTTGTTGTTGATTGTTTAATTAAACA
AGGTGTTACACATGTTTTCGGTATTCCAGGAGCAAAGATTGACTCTGTATTTGATGTACTGCAAGAAAGAGGACCAGAGT
TAATTGTTTGTCGTCATGAACAAAATGCAGCATTTATGGCAGCTGCTATTGGTAGATTAACAGGAAAACCGGGCGTATGT
CTTGTAACTTCAGGACCAGGGACATCAAATTTAGCGACAGGTCTTGTTACTGCGAATGCGGAGAGTGATCCCGTTGTTGC
TTTAGCTGGTGCAGTTCCGCGTACGGATCGATTAAAACGTACACATCAATCTATGGATAATGCTGCACTATTCGAACCAA
TCACAAAATATAGCGTAGAAGTAGAGCATCCTGATAATGTGCCAGAAGCATTATCAAATGCATTCCGAAGTGCGACTTCT
ACAAATCCAGGAGCAACTTTAGTAAGTTTGCCGCAAGACGTTATGACTGCGGAAACGACTGTAGAGTCTATCGGTGCGCT
TTCTAAGCCACAGCTTGGAATCGCTCCCACACATGATATTACATATGTAGTAGATAAAATAAAAGCAGCGAAATTACCAG
TTATTTTACTCGGTATGAGAGCGAGCACAAATGAAGTGACGAAAGCCGTTCGTAAATTAATTGCGGATACAGAACTTCCT
GTCGTTGAAACATATCAAGCGGCTGGTGCCATTTCACGTGAGTTAGAAGATCATTTCTTCGGCCGTGTTGGACTATTCCG
TAACCAACCAGGTGATATTTTACTAGAAGAGGCAGATCTTGTTATTTCTATCGGTTATGATCCAATTGAGTATGATCCAA
AGTTCTGGAATAAACTTGGAGACAGAACGATTATTCATCTTGATGACCATCAAGCAGATATAGATCATGATTACCAACCA
GAGCGTGAATTAATTGGTGATATTGCCTTAACAGTAAATAGCATCGCAGAAAAGTTACCGAAACTTGTGTTAAGTACGAA
ATCAGAAGCAGTGTTAGAACGATTACGCGCGAAATTATCAGAACAAGCAGAAGTTCCAAATCGTCCTTCAGAAGGTGTTA
CACATCCGCTTCAAGTGATTCGTACACTTCGTTCTTTAATTAGTGACGACACAACCGTTACATGTGACATCGGTTCCCAT
TCTATTTGGATGGCGAGATGTTTCCGTTCTTATGAACCACGTAGATTATTATTTAGTAACGGTATGCAGACGTTAGGTGT
TGCACTTCCTTGGGCAATTGCTGCTACTTTAGTAGAACCAGGTAAAAAAGTAGTTTCCGTATCAGGTGACGGTGGTTTCT
TATTCTCAGCGATGGAGTTAGAAACGGCGGTACGTTTAAATTCTCCAATCGTCCATCTTGTTTGGAGAGACGGCACATAT
GATATGGTTGCATTCCAACAAATGATGAAATACGGCAGAACATCAGCTACAGAGTTTGGTGATGTTGATCTTGTTAAATA
TGCGGAAAGTTTCGGGGCGTTAGGTCTTCGTGTTAACACGCCTGATGAATTAGAAGGGGTATTGAAAGAAGCACTAGCAG
CAGACGGCCCTGTCATTATTGATATTCCAATTGACTATCGTGACAACATTAAATTAAGCGAAAAATTATTACCAAACCAA
TTAAACTAA

Upstream 100 bases:

>100_bases
CATTTGTAATATAATGATAAATGATACTTCACCACGTTAATTAACTAGGTGAAATTAAAAATCTTTATTACACAAATTAT
GAAACAAAGGAATGATTAGT

Downstream 100 bases:

>100_bases
TGGAGGCAGATTTGAGATGACTGTTGCGCAATTAATTGATATTGATGCAAAAAAAACGAAAACGAGTAATGAAGTATATC
AAACATCTACAATGCTTGCG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKPGVC
LVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATS
TNPGATLVSLPQDVMTAETTVESIGALSKPQLGIAPTHDITYVVDKIKAAKLPVILLGMRASTNEVTKAVRKLIADTELP
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHDYQP
ERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSH
SIWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
DMVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKEALAADGPVIIDIPIDYRDNIKLSEKLLPNQ
LN

Sequences:

>Translated_562_residues
MSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKPGVC
LVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATS
TNPGATLVSLPQDVMTAETTVESIGALSKPQLGIAPTHDITYVVDKIKAAKLPVILLGMRASTNEVTKAVRKLIADTELP
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHDYQP
ERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSH
SIWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
DMVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKEALAADGPVIIDIPIDYRDNIKLSEKLLPNQ
LN
>Mature_561_residues
STGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHEQNAAFMAAAIGRLTGKPGVCL
VTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKRTHQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATST
NPGATLVSLPQDVMTAETTVESIGALSKPQLGIAPTHDITYVVDKIKAAKLPVILLGMRASTNEVTKAVRKLIADTELPV
VETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLGDRTIIHLDDHQADIDHDYQPE
RELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLSEQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHS
IWMARCFRSYEPRRLLFSNGMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTYD
MVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKEALAADGPVIIDIPIDYRDNIKLSEKLLPNQL
N

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=26.9026548672566, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI21361361, Length=596, Percent_Identity=25.503355704698, Blast_Score=149, Evalue=8e-36,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=29.4014084507042, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=29.5081967213115, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1786717, Length=574, Percent_Identity=26.6550522648084, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1787096, Length=536, Percent_Identity=26.865671641791, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=27.158273381295, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=573, Percent_Identity=26.3525305410122, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=553, Percent_Identity=27.4864376130199, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=556, Percent_Identity=27.6978417266187, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=562, Percent_Identity=28.2918149466192, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320816, Length=539, Percent_Identity=27.6437847866419, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6323163, Length=548, Percent_Identity=23.3576642335766, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=557, Percent_Identity=26.2118491921005, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61021; Mature: 60890

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHE
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCEEEEECC
QNAAFMAAAIGRLTGKPGVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKR
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHH
THQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATSTNPGATLVSLPQDVMTAETT
HHHHCCCHHHHCCHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
VESIGALSKPQLGIAPTHDITYVVDKIKAAKLPVILLGMRASTNEVTKAVRKLIADTELP
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCC
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCHHHHHHCC
DRTIIHLDDHQADIDHDYQPERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLS
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHSIWMARCFRSYEPRRLLFSN
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHC
GMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCH
DMVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKEALAADGPVII
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCEEE
DIPIDYRDNIKLSEKLLPNQLN
EEECCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
STGVKANDVKTKTKGADLVVDCLIKQGVTHVFGIPGAKIDSVFDVLQERGPELIVCRHE
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHCCCCEEEEECC
QNAAFMAAAIGRLTGKPGVCLVTSGPGTSNLATGLVTANAESDPVVALAGAVPRTDRLKR
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHH
THQSMDNAALFEPITKYSVEVEHPDNVPEALSNAFRSATSTNPGATLVSLPQDVMTAETT
HHHHCCCHHHHCCHHHCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHH
VESIGALSKPQLGIAPTHDITYVVDKIKAAKLPVILLGMRASTNEVTKAVRKLIADTELP
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCC
VVETYQAAGAISRELEDHFFGRVGLFRNQPGDILLEEADLVISIGYDPIEYDPKFWNKLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCHHHHHHCC
DRTIIHLDDHQADIDHDYQPERELIGDIALTVNSIAEKLPKLVLSTKSEAVLERLRAKLS
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EQAEVPNRPSEGVTHPLQVIRTLRSLISDDTTVTCDIGSHSIWMARCFRSYEPRRLLFSN
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHC
GMQTLGVALPWAIAATLVEPGKKVVSVSGDGGFLFSAMELETAVRLNSPIVHLVWRDGTY
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCH
DMVAFQQMMKYGRTSATEFGDVDLVKYAESFGALGLRVNTPDELEGVLKEALAADGPVII
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCEEE
DIPIDYRDNIKLSEKLLPNQLN
EEECCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]