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The map label for this gene is pflA [H]

Identifier: 218233688

GI number: 218233688

Start: 499732

End: 500463

Strand: Direct

Name: pflA [H]

Synonym: BCB4264_A0513

Alternate gene names: 218233688

Gene position: 499732-500463 (Clockwise)

Preceding gene: 218230943

Following gene: 218235948

Centisome position: 9.22

GC content: 36.2

Gene sequence:

>732_bases
ATGGTAAAAGGAAGAATACATTCTGTAGAGTCTTGTGGTACTGTTGATGGCCCGGGGATTCGTTATGTCATATTTACACA
AGGGTGTTTATTACGTTGTCAATATTGTCATAATGCTGATACGTGGGAAATCGGTAAAGGTAAAGAAATAACAGTTGAAG
AAGTGATGCAGGATGTGACATGTTACCTTCCATTTATTGAAGCTTCCGGAGGCGGTATAACAGTTAGCGGTGGAGAGCCA
TTATTACAGCTAGACTTTTTAATTGAGTTGTTTAAAAAGTGTAAAGCGGCTGGTATCCATACCACAATCGACTCTTCTGG
TGGTTGTTATTCTGAGGAACCAGAATTCCAAAATAAGCTAGATATTTTAATGGATTATACAGATTTAGTTTTATTGGATC
TGAAACATATCGATTCCAAAAAGCATCGTAAATTAACAGGGAAACCAAATGAACATATTTTACAATTTGCTCGTTATTTA
TCGGATAAGAATAAACCGATTTGGGTACGACACGTATTAGTTCCTGGTGTTACGGATAATGAAGAGGATCTACAAAAACT
ATCTAGCTTTATTCAAAGTCTGTCTAATGTTCAAAAAGTTGAAGTGTTACCATATCATAAGCTTGGTGTATATAAATGGG
AGGCGCTTGGACACAAGTATCCACTCGCAAATGTAGAACCACCTACTGAGAAAAATGTAGAACATGCTAAATATATTTTA
CAAGCGGTCTAA

Upstream 100 bases:

>100_bases
ATTAACCGTACAATGCATGAAAGCATGTAAATGAAAAAAGGTGTCCCTTCGCTCTTAATATATAGCGAAGGGAGGCTGTT
TCAATAAAGGAGGAAGTAAC

Downstream 100 bases:

>100_bases
TCCAAATATTGGATTAGGCTTTTTGCTTTCTTTACAATAAAATCTAGAGTATATATTGATAAAAGAGTATACTGAAAGTA
GAATATCCATAATTTTGTAC

Product: pyruvate formate-lyase-activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVTCYLPFIEASGGGITVSGGEP
LLQLDFLIELFKKCKAAGIHTTIDSSGGCYSEEPEFQNKLDILMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYL
SDKNKPIWVRHVLVPGVTDNEEDLQKLSSFIQSLSNVQKVEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEHAKYIL
QAV

Sequences:

>Translated_243_residues
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVTCYLPFIEASGGGITVSGGEP
LLQLDFLIELFKKCKAAGIHTTIDSSGGCYSEEPEFQNKLDILMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYL
SDKNKPIWVRHVLVPGVTDNEEDLQKLSSFIQSLSNVQKVEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEHAKYIL
QAV
>Mature_243_residues
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVTCYLPFIEASGGGITVSGGEP
LLQLDFLIELFKKCKAAGIHTTIDSSGGCYSEEPEFQNKLDILMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYL
SDKNKPIWVRHVLVPGVTDNEEDLQKLSSFIQSLSNVQKVEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEHAKYIL
QAV

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=240, Percent_Identity=49.1666666666667, Blast_Score=264, Evalue=4e-72,
Organism=Escherichia coli, GI1790389, Length=268, Percent_Identity=25.7462686567164, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1790839, Length=247, Percent_Identity=29.5546558704453, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI226510931, Length=273, Percent_Identity=26.007326007326, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27371; Mature: 27371

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVT
CCCCCCCCHHCCCCCCCCCEEEEEEECCHHEEEHHHCCCCCEECCCCCEEEHHHHHHHHH
CYLPFIEASGGGITVSGGEPLLQLDFLIELFKKCKAAGIHTTIDSSGGCYSEEPEFQNKL
HHHHEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHH
DILMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYLSDKNKPIWVRHVLVPGVTDN
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
EEDLQKLSSFIQSLSNVQKVEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEHAKYIL
HHHHHHHHHHHHHHHCCCEEEECCCHHCCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QAV
HCC
>Mature Secondary Structure
MVKGRIHSVESCGTVDGPGIRYVIFTQGCLLRCQYCHNADTWEIGKGKEITVEEVMQDVT
CCCCCCCCHHCCCCCCCCCEEEEEEECCHHEEEHHHCCCCCEECCCCCEEEHHHHHHHHH
CYLPFIEASGGGITVSGGEPLLQLDFLIELFKKCKAAGIHTTIDSSGGCYSEEPEFQNKL
HHHHEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHH
DILMDYTDLVLLDLKHIDSKKHRKLTGKPNEHILQFARYLSDKNKPIWVRHVLVPGVTDN
HHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
EEDLQKLSSFIQSLSNVQKVEVLPYHKLGVYKWEALGHKYPLANVEPPTEKNVEHAKYIL
HHHHHHHHHHHHHHHCCCEEEECCCHHCCCEEHHHCCCCCCCCCCCCCCCCCHHHHHHHH
QAV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]