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The map label for this gene is yxeI [H]

Identifier: 218233617

GI number: 218233617

Start: 1021084

End: 1022070

Strand: Direct

Name: yxeI [H]

Synonym: BCB4264_A1046

Alternate gene names: 218233617

Gene position: 1021084-1022070 (Clockwise)

Preceding gene: 218233435

Following gene: 218232580

Centisome position: 18.84

GC content: 37.39

Gene sequence:

>987_bases
ATGTGTACTAGTTTGACGTTACAAACTAAAAATTTCCAACATCTTTTTGCAAGAACAATGGATTTCACATTAGATATGAA
TCAGGAAGTAATAATCATCCCTCGGCGTTATCAGTGGAATAATATAACTGGTGAAACGATCCGAGCGAAAAAGGCTGTTG
TCGGAATGGGAATTAATCATGAAGGAAGGGTCATTATGGCGGACGGAGTAAATGAAGCTGGTATGACGTGTGCTACTCTC
TATTTCCCGGGATTCGCTACTTATAGTAACAGTATAGATAACAATAAAACGAATTTAGCGCCATTTGATTTTGTAGCTTG
GAGCCTCACGCAGTTTAATTCTGTTGAAGAGTTAAAAAAATCTGTTGATAACATTTCTCTTATAGATATACCATTACCAG
TTTTAGGAATTGCTCCGCCGCTACATTGGATTTTAGCAGATAAATGGGGAGATTGTATTGTACTTGAGCCGACAACTGAT
GGACTAAAAATGTATGACAATCCACTAGGAGTGATGACTAATAGTCCGGAGTTTAATTGGCATTTACAAAATTTAAGACA
ATATATTGGTCTTAAATCACAGCCATTTGCGCTAACGGAGTGGGGCGATGTACCATTAAGTGCTTTTGGACAAGGTTCAG
GATCGATGGGACTTCCTGGAGATTTCACACCGCCATCAAGATTTGTGCGGGCAGCGTATGGCAAACAAAACATTCAAGGT
ATAGAGAACGAAGAAGAGGGGATATCGGCTATTTTTCATATTTTATCAAATTGTGAGGTCCCTAAAGGGGCGGTAATAAC
AGAAGACGGAACATTAGATAATACCATCTATACGAGTGCAATGTGTATGGAGTCTGGAACATATTATTACCATACTTACG
ATTGTAGACAAATTATAGCTATTCATTTATTCCATGAAAATTTAGATGCAACTGAGATTAAAACTTATCCGTTTCAGCGG
AAACAAAAAATATTTTATGAAAACTGA

Upstream 100 bases:

>100_bases
TGGGTGGTTATTTTGTTAGTGATAAATAGGGTTTTTAATTTACGTATTTATCAGAATTTTATAAAATGTGTATATAACTA
TAAAATGGGGGGATTTCTTC

Downstream 100 bases:

>100_bases
AGCGCCCCTGAAAAAGTGAGACTAATAAAAACACCTCCATTTAATTTGCATACTTAGTATGCGAATTTAGGAGGTGTTTT
TTATCATTTACCAATATTCA

Product: choloylglycine hydrolase

Products: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MCTSLTLQTKNFQHLFARTMDFTLDMNQEVIIIPRRYQWNNITGETIRAKKAVVGMGINHEGRVIMADGVNEAGMTCATL
YFPGFATYSNSIDNNKTNLAPFDFVAWSLTQFNSVEELKKSVDNISLIDIPLPVLGIAPPLHWILADKWGDCIVLEPTTD
GLKMYDNPLGVMTNSPEFNWHLQNLRQYIGLKSQPFALTEWGDVPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
IENEEEGISAIFHILSNCEVPKGAVITEDGTLDNTIYTSAMCMESGTYYYHTYDCRQIIAIHLFHENLDATEIKTYPFQR
KQKIFYEN

Sequences:

>Translated_328_residues
MCTSLTLQTKNFQHLFARTMDFTLDMNQEVIIIPRRYQWNNITGETIRAKKAVVGMGINHEGRVIMADGVNEAGMTCATL
YFPGFATYSNSIDNNKTNLAPFDFVAWSLTQFNSVEELKKSVDNISLIDIPLPVLGIAPPLHWILADKWGDCIVLEPTTD
GLKMYDNPLGVMTNSPEFNWHLQNLRQYIGLKSQPFALTEWGDVPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
IENEEEGISAIFHILSNCEVPKGAVITEDGTLDNTIYTSAMCMESGTYYYHTYDCRQIIAIHLFHENLDATEIKTYPFQR
KQKIFYEN
>Mature_328_residues
MCTSLTLQTKNFQHLFARTMDFTLDMNQEVIIIPRRYQWNNITGETIRAKKAVVGMGINHEGRVIMADGVNEAGMTCATL
YFPGFATYSNSIDNNKTNLAPFDFVAWSLTQFNSVEELKKSVDNISLIDIPLPVLGIAPPLHWILADKWGDCIVLEPTTD
GLKMYDNPLGVMTNSPEFNWHLQNLRQYIGLKSQPFALTEWGDVPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
IENEEEGISAIFHILSNCEVPKGAVITEDGTLDNTIYTSAMCMESGTYYYHTYDCRQIIAIHLFHENLDATEIKTYPFQR
KQKIFYEN

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: 3.5.1.24

Molecular weight: Translated: 36922; Mature: 36922

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTSLTLQTKNFQHLFARTMDFTLDMNQEVIIIPRRYQWNNITGETIRAKKAVVGMGINH
CCCEEEEECCCHHHHHHHHHEEEEECCCCEEEEECEEECCCCCCCCEEHHHEEEEECCCC
EGRVIMADGVNEAGMTCATLYFPGFATYSNSIDNNKTNLAPFDFVAWSLTQFNSVEELKK
CCCEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
SVDNISLIDIPLPVLGIAPPLHWILADKWGDCIVLEPTTDGLKMYDNPLGVMTNSPEFNW
HHCCEEEEEECCCHHHCCCCCEEEEECCCCCEEEECCCCCCCEEECCCCCEEECCCCCCE
HLQNLRQYIGLKSQPFALTEWGDVPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
EHHHHHHHHCCCCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
IENEEEGISAIFHILSNCEVPKGAVITEDGTLDNTIYTSAMCMESGTYYYHTYDCRQIIA
CCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHEEEECCCEEEEEEECHHEEEE
IHLFHENLDATEIKTYPFQRKQKIFYEN
EEEECCCCCCCEEECCCCCHHCCCCCCC
>Mature Secondary Structure
MCTSLTLQTKNFQHLFARTMDFTLDMNQEVIIIPRRYQWNNITGETIRAKKAVVGMGINH
CCCEEEEECCCHHHHHHHHHEEEEECCCCEEEEECEEECCCCCCCCEEHHHEEEEECCCC
EGRVIMADGVNEAGMTCATLYFPGFATYSNSIDNNKTNLAPFDFVAWSLTQFNSVEELKK
CCCEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
SVDNISLIDIPLPVLGIAPPLHWILADKWGDCIVLEPTTDGLKMYDNPLGVMTNSPEFNW
HHCCEEEEEECCCHHHCCCCCEEEEECCCCCEEEECCCCCCCEEECCCCCEEECCCCCCE
HLQNLRQYIGLKSQPFALTEWGDVPLSAFGQGSGSMGLPGDFTPPSRFVRAAYGKQNIQG
EHHHHHHHHCCCCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
IENEEEGISAIFHILSNCEVPKGAVITEDGTLDNTIYTSAMCMESGTYYYHTYDCRQIIA
CCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHEEEECCCEEEEEEECHHEEEE
IHLFHENLDATEIKTYPFQRKQKIFYEN
EEEECCCCCCCEEECCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine; H2O

Specific reaction: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]