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The map label for this gene is alkK [H]

Identifier: 218233506

GI number: 218233506

Start: 3654459

End: 3656072

Strand: Reverse

Name: alkK [H]

Synonym: BCB4264_A3738

Alternate gene names: 218233506

Gene position: 3656072-3654459 (Counterclockwise)

Preceding gene: 218234240

Following gene: 218232129

Centisome position: 67.47

GC content: 40.02

Gene sequence:

>1614_bases
ATGATGATGAATGTACCGCTAACAATTAGTTCTATGATGGAAAGAGCAGAGAAACTGTTTTCGAAGAAAGAAATTGTTTC
ACGGACGCATGATACAGTTACAACGTTAACGTATAAGCAGTTAGGGGAAAGGACGAGAAGACTCTCCAGTGCGTTGAAAA
AGCTTGGAATTAAAGAAGGTGAACGTGTAGGAACATTAGCGTGGAATCATCATCGACATGTGGAGGCGTATTTTGCTATT
CCTGGTATTGCTTCCGTTTTGCACACAATTAACATTCGTTTATCTCCTCAACATATTTCATATATTATTCAGCACGCAGA
AGACCGAATTCTACTTATAGATGAAGATCTCGTACCGCTTGTTGAAAATATTCAATCACAATTATCAACTGTACAAGCTT
ACATTATTATGACTGATAAAGATGAACTCCCAAACACTACGCTGAAGCCTGTATATCATTATGAAAAGCTATTAGAAGAA
GGCGATCCAAATTTCCAATTTGTAAAAGATATTGATGAAAATACACCAGCTGGTATGTGTTATACGTCAGCGACTACAGG
AAATCCGAAAGGTGTTGTATATACGCATCGTAGTACTGTATTACACTGTATGGCACTCGGTTTAGCGGATACAGCTGCTT
TATCGGAAAGTGATGCTGCAATGGCGATTGTACCGATGTTCCATGTGAACGCTTGGGGACTTCCTTTCGCTGCTACTTGG
TTTGGGTCAAAACAAGTTCTTCCAGGACCGATGTTTACTCCGAAAATTTTATTAGAGATGATTCAATCAGAAAAAGTGAC
GTTAGCTGCGGGTGTGCCGACAATTTGGCTCGGCGTATTACAAGAGTTAGAAAATAATAGTTACGATTTATCTAGTATGA
CGAGAATACTATGCGGAGGTGCAGCTGCGCCGAAAAGTGTTATTAAAGCATTTGAACAGAAACATAATGTTCCGTTCATA
CATGCATATGGTATGACTGAAACGAGTCCACTCGTAACACTTGCGCGTTTAAAAAGTTATGAAACGGAAGTATCGTATGA
AGAGCAACTAGAAATTCGCTCTAAACAAGGATATCTTGTACCTGGTGTCGAGATGAAAGTAGTCGGTACAAATGGTGACG
TGAAGTGGGATGGTACTGAGATGGGAGAATTATGTTTACGAGCACCTTGGATCGCTGAAAGCTATTATAACGATGATCGT
ACTGTCGAAGGTTTTCGCGATGGATGGTTATATACTGGTGATGTTGTCACAGTGGATGAGGAAGGTTGCGTGAAAATTGT
TGACCGTACGAAGGATGTTATTAAAAGCGGGGGAGAATGGATTTCTTCGGTCGATCTTGAAAATGCTTTAATGGCACATG
ATGCTATATTTGAAGCGGCTGTCGTTGCAGTTCCTCACCCTCAGTGGCAAGAGCGACCAATTGCCTGCGTTGTTCAAAAG
AAAAATAGTACGGTTACAAAAGAAGAAATATATGAGTTTTTAAAACCACAGTTTGCGAAGTGGTGGTTGCCAGACGATAT
TGTCTTTATGGAAGAAATACCGAAAACATCTGTTGGGAAGTTTTTAAAACAGGCGCTTCGGAAAGAACTAGAGCATTTGC
ATAGAGAGAAATAA

Upstream 100 bases:

>100_bases
GAAATAATTCAGAAAATACAGAAAAATGTCGTAATCATTTGCGGAAGTCTGTTATAATAAACGAAAAAATGAAAACGTTT
TAAAGGGGGACATAACCAAT

Downstream 100 bases:

>100_bases
GTGAACTTCTATTTGTAAACTCTAATACATTAACGGTTGACAAAAGAGGGGTGGAAAGATAATATAATTTTTGCGATATG
TTATCTAAAAACAATTTTAA

Product: medium-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERVGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLVENIQSQLSTVQAYIIMTDKDELPNTTLKPVYHYEKLLEE
GDPNFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTETSPLVTLARLKSYETEVSYEEQLEIRSKQGYLVPGVEMKVVGTNGDVKWDGTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPIACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHREK

Sequences:

>Translated_537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERVGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLVENIQSQLSTVQAYIIMTDKDELPNTTLKPVYHYEKLLEE
GDPNFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTETSPLVTLARLKSYETEVSYEEQLEIRSKQGYLVPGVEMKVVGTNGDVKWDGTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPIACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHREK
>Mature_537_residues
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEGERVGTLAWNHHRHVEAYFAI
PGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPLVENIQSQLSTVQAYIIMTDKDELPNTTLKPVYHYEKLLEE
GDPNFQFVKDIDENTPAGMCYTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGGAAAPKSVIKAFEQKHNVPFI
HAYGMTETSPLVTLARLKSYETEVSYEEQLEIRSKQGYLVPGVEMKVVGTNGDVKWDGTEMGELCLRAPWIAESYYNDDR
TVEGFRDGWLYTGDVVTVDEEGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPIACVVQK
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHREK

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=555, Percent_Identity=28.4684684684685, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI187761345, Length=550, Percent_Identity=26.5454545454545, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI187761343, Length=550, Percent_Identity=26.5454545454545, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI38505220, Length=509, Percent_Identity=25.3438113948919, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI42544132, Length=512, Percent_Identity=24.4140625, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI157311624, Length=511, Percent_Identity=23.679060665362, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI157311622, Length=511, Percent_Identity=23.679060665362, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI28416953, Length=539, Percent_Identity=23.747680890538, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI58082049, Length=517, Percent_Identity=23.21083172147, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=22.4806201550388, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=21.9844357976654, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI83745141, Length=518, Percent_Identity=22.7799227799228, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1788107, Length=521, Percent_Identity=26.1036468330134, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI145693145, Length=364, Percent_Identity=32.1428571428571, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI221142682, Length=513, Percent_Identity=23.9766081871345, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1786810, Length=526, Percent_Identity=24.1444866920152, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789201, Length=510, Percent_Identity=23.1372549019608, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1790505, Length=544, Percent_Identity=22.4264705882353, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788595, Length=499, Percent_Identity=22.4448897795591, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=514, Percent_Identity=25.4863813229572, Blast_Score=166, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17559526, Length=360, Percent_Identity=32.2222222222222, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17558820, Length=515, Percent_Identity=25.631067961165, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=25.891181988743, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=25.891181988743, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=25.891181988743, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=537, Percent_Identity=26.2569832402235, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=510, Percent_Identity=24.3137254901961, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=24.0776699029126, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=505, Percent_Identity=24.3564356435644, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17560140, Length=506, Percent_Identity=22.7272727272727, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=520, Percent_Identity=24.6153846153846, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=372, Percent_Identity=22.8494623655914, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319699, Length=364, Percent_Identity=28.021978021978, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21355181, Length=500, Percent_Identity=28, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=26.6787658802178, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI18859661, Length=359, Percent_Identity=27.8551532033426, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21356441, Length=526, Percent_Identity=23.574144486692, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648255, Length=513, Percent_Identity=22.6120857699805, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648253, Length=507, Percent_Identity=21.8934911242604, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24648260, Length=496, Percent_Identity=22.3790322580645, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922652, Length=520, Percent_Identity=23.0769230769231, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653035, Length=516, Percent_Identity=22.4806201550388, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24656500, Length=531, Percent_Identity=21.6572504708098, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648257, Length=543, Percent_Identity=22.6519337016575, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281365686, Length=513, Percent_Identity=19.1033138401559, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI161076582, Length=528, Percent_Identity=22.1590909090909, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI20130357, Length=523, Percent_Identity=21.6061185468451, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356947, Length=521, Percent_Identity=20.5374280230326, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21358303, Length=496, Percent_Identity=20.1612903225806, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60413; Mature: 60413

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEG
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ERVGTLAWNHHRHVEAYFAIPGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPL
CEEEEEEECCCCCEEEEEECCHHHHHHHHHEEEECHHHHHHHHHHCCCCEEEECCCHHHH
VENIQSQLSTVQAYIIMTDKDELPNTTLKPVYHYEKLLEEGDPNFQFVKDIDENTPAGMC
HHHHHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGG
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
AAAPKSVIKAFEQKHNVPFIHAYGMTETSPLVTLARLKSYETEVSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEE
PGVEMKVVGTNGDVKWDGTEMGELCLRAPWIAESYYNDDRTVEGFRDGWLYTGDVVTVDE
CCCEEEEEECCCCEEECCCHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPIACVVQK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHREK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMMNVPLTISSMMERAEKLFSKKEIVSRTHDTVTTLTYKQLGERTRRLSSALKKLGIKEG
CEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ERVGTLAWNHHRHVEAYFAIPGIASVLHTINIRLSPQHISYIIQHAEDRILLIDEDLVPL
CEEEEEEECCCCCEEEEEECCHHHHHHHHHEEEECHHHHHHHHHHCCCCEEEECCCHHHH
VENIQSQLSTVQAYIIMTDKDELPNTTLKPVYHYEKLLEEGDPNFQFVKDIDENTPAGMC
HHHHHHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCCCEE
YTSATTGNPKGVVYTHRSTVLHCMALGLADTAALSESDAAMAIVPMFHVNAWGLPFAATW
EECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCEEEEE
FGSKQVLPGPMFTPKILLEMIQSEKVTLAAGVPTIWLGVLQELENNSYDLSSMTRILCGG
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
AAAPKSVIKAFEQKHNVPFIHAYGMTETSPLVTLARLKSYETEVSYEEQLEIRSKQGYLV
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEE
PGVEMKVVGTNGDVKWDGTEMGELCLRAPWIAESYYNDDRTVEGFRDGWLYTGDVVTVDE
CCCEEEEEECCCCEEECCCHHHHHHHCCCHHHHHHCCCCCCCCHHCCCCEEECCEEEECC
EGCVKIVDRTKDVIKSGGEWISSVDLENALMAHDAIFEAAVVAVPHPQWQERPIACVVQK
CCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEC
KNSTVTKEEIYEFLKPQFAKWWLPDDIVFMEEIPKTSVGKFLKQALRKELEHLHREK
CCCCCCHHHHHHHHCCHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]