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The map label for this gene is ntcA [H]

Identifier: 218233443

GI number: 218233443

Start: 401713

End: 402402

Strand: Direct

Name: ntcA [H]

Synonym: BCB4264_A0424

Alternate gene names: 218233443

Gene position: 401713-402402 (Clockwise)

Preceding gene: 218231136

Following gene: 218234214

Centisome position: 7.41

GC content: 31.01

Gene sequence:

>690_bases
ATGAATAGACGGAACGTAGTGAAAGACTTAAAGCAGTTCGAATTATTTGCTCATTTAACAGAAAAAAAATTGAAAGGCTT
AACAGAGTTTGTTTATTGGCGAACTTATAAAAAAGGTCAATTTTTATTTTTAGAAGGGGATTCAAGGGAAAGAATTTACT
TCATGTTAGATGGTTTTGTAAAGTTAGAGCGGGTTAATCAAAATGGGAACTTATTATACGATGACTATGTAAAGCGGTAT
TCTATTTTTCCTTATGGTGGTATGTTTACAGACAGAGGATATAACTATACAGCAGAAGCAATGACGGATGTGGAGGTATA
TTATATTCCAACGGTAATCTTTGAAGATATGGTAAAGTCCAGCAGAACGCAATTGATGTACGTTGTTCAGCAATTATCAT
CTATATTAAAATTAAACGAAAATCGAGTGCAAAATATTACAATTCCTAATGCACAAGATCGTGTTATCCAAACGTTAAAT
TATTTAATGCAGGATTTAGGAGAACAGAGTGGAGAAAAGATTGTAATTTCATGTCCACTTACAACAATTGAAATATCCAA
AATATCTGGAACATCTCGAGAGACAGTTAGTAGCGTATTAAAACAATTAAAAAATGATAGCATTGTTACAATTTTAGATA
AAAAAATTACAATACATGATCCAACATATTTTGAAGAAATCTCTATGTGA

Upstream 100 bases:

>100_bases
ATGATGAAATCTGAATATACAAGTTAATTATGATAAAATTCAAATTGAATACGCTTTCTTGATTTTTCGCTCCAACATAC
AGCAAGAGAGAGGGAATTTT

Downstream 100 bases:

>100_bases
AAATTGTATATTGATTTTTTAATTCATAGTGTATATTAATAAGGTTCTTTATATATTTATAAGGAACTTTTTTACGTTAT
CCTCTATAAAAAGATTGTAT

Product: transcriptional regulator, Crp family

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNRRNVVKDLKQFELFAHLTEKKLKGLTEFVYWRTYKKGQFLFLEGDSRERIYFMLDGFVKLERVNQNGNLLYDDYVKRY
SIFPYGGMFTDRGYNYTAEAMTDVEVYYIPTVIFEDMVKSSRTQLMYVVQQLSSILKLNENRVQNITIPNAQDRVIQTLN
YLMQDLGEQSGEKIVISCPLTTIEISKISGTSRETVSSVLKQLKNDSIVTILDKKITIHDPTYFEEISM

Sequences:

>Translated_229_residues
MNRRNVVKDLKQFELFAHLTEKKLKGLTEFVYWRTYKKGQFLFLEGDSRERIYFMLDGFVKLERVNQNGNLLYDDYVKRY
SIFPYGGMFTDRGYNYTAEAMTDVEVYYIPTVIFEDMVKSSRTQLMYVVQQLSSILKLNENRVQNITIPNAQDRVIQTLN
YLMQDLGEQSGEKIVISCPLTTIEISKISGTSRETVSSVLKQLKNDSIVTILDKKITIHDPTYFEEISM
>Mature_229_residues
MNRRNVVKDLKQFELFAHLTEKKLKGLTEFVYWRTYKKGQFLFLEGDSRERIYFMLDGFVKLERVNQNGNLLYDDYVKRY
SIFPYGGMFTDRGYNYTAEAMTDVEVYYIPTVIFEDMVKSSRTQLMYVVQQLSSILKLNENRVQNITIPNAQDRVIQTLN
YLMQDLGEQSGEKIVISCPLTTIEISKISGTSRETVSSVLKQLKNDSIVTILDKKITIHDPTYFEEISM

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26790; Mature: 26790

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRNVVKDLKQFELFAHLTEKKLKGLTEFVYWRTYKKGQFLFLEGDSRERIYFMLDGFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCEEEEEEECEE
KLERVNQNGNLLYDDYVKRYSIFPYGGMFTDRGYNYTAEAMTDVEVYYIPTVIFEDMVKS
EEEEECCCCCEEEHHHHHHEECCCCCCEECCCCCCEEHHHHCCEEEEEEHHHHHHHHHHH
SRTQLMYVVQQLSSILKLNENRVQNITIPNAQDRVIQTLNYLMQDLGEQSGEKIVISCPL
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
TTIEISKISGTSRETVSSVLKQLKNDSIVTILDKKITIHDPTYFEEISM
EEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECEEEEECCCHHHHCCC
>Mature Secondary Structure
MNRRNVVKDLKQFELFAHLTEKKLKGLTEFVYWRTYKKGQFLFLEGDSRERIYFMLDGFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCEEEEEEECEE
KLERVNQNGNLLYDDYVKRYSIFPYGGMFTDRGYNYTAEAMTDVEVYYIPTVIFEDMVKS
EEEEECCCCCEEEHHHHHHEECCCCCCEECCCCCCEEHHHHCCEEEEEEHHHHHHHHHHH
SRTQLMYVVQQLSSILKLNENRVQNITIPNAQDRVIQTLNYLMQDLGEQSGEKIVISCPL
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
TTIEISKISGTSRETVSSVLKQLKNDSIVTILDKKITIHDPTYFEEISM
EEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECEEEEECCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]