| Definition | Bacillus cereus B4264, complete genome. |
|---|---|
| Accession | NC_011725 |
| Length | 5,419,036 |
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The map label for this gene is colA [H]
Identifier: 218233315
GI number: 218233315
Start: 3547272
End: 3550187
Strand: Reverse
Name: colA [H]
Synonym: BCB4264_A3636
Alternate gene names: 218233315
Gene position: 3550187-3547272 (Counterclockwise)
Preceding gene: 218233033
Following gene: 218232923
Centisome position: 65.51
GC content: 34.91
Gene sequence:
>2916_bases ATGAAAGGCTATTCAAAGAAAATGTTAGTAGGGGTAAGTTTTGCTAGTTTAATGTTAGGGAGTTTTCAAGGGGTAAGTTT GGCAGAAGATACTAAGGGAGAGCAAGTTTCTTATCGAAATGTGCTCAAAATGGAGCCAGTCGGTGTACAACTACCAGTAC AAGAATTAGCTCATTCATCGAAAGTGCTCGAAAGCAAGTCTTTTGAGAAAAGGTTACAATTTGCCGATTTATCACAAAGA CCACCTGAGGTGAAAAAAGAAAGTAAGCAATTAGCTGTAGCGAAAACTTATACAATTGCCGAATTAAATCAATTAAGCAA TCAGCAGTTAGTAGATTTACTTGTAACAATTGATTGGGAGCAAATTACGGGGCTATTTCAGTTTAATACAGACAGTCTAG CATTCTATCAAAATGATAGTAGGATGCAGGCGATTATTGATAAATTGAAGCAGCAAGGACAAGCCTATACGAAGGATGAT TCAAAAGGGATTGAGACATTAGTAGAGGTATTACGTTCAGGATTTTATTTAGGATTTTATAATTCGGAACTTAGTAAATT AAATGAACGGAGTTATCATGATAAATGCTTACCTGCATTAAAAGCGATGGCTAACAATCCAAATTTCAAGCTGGGTACAT TAGAGCAAAATAGAGTGGTATCATCATACGGAAAGTTAATAGGAAACGCTTCGAGCGATGTGGAAACGATAACATCGGCT GCAAAGATTTTTAAACAATATAATGATAATTTTTCTACATTAGTAGATAATCTTTCAGCTGGAAATGCTATTTACGATAT TATGCAAGGTGTTGATTACGACATTCAATCGTATTTGTACGATACGAGGAAAGCACCGAAAGATACAGTATGGTATCAGA AAATAGATAGTTATATTAATGAATTAAGTCGTTTTGCATTAATGGGAACGATTACAGCAAAAAACGGTTGGCTTATTAAT AATGGCATTTATTATACAGGTAGACTCGGTATGTTCCATAGTACAGGAACTAAAGGGTTACAAGTTGTAACAGATGCGAT GAAAATGTATCCGTATTTAGGGGAGCAATATTTCGTAGCTGCTGAGCAAATTGCAACGAATTATGGCGGAAAAGATGCAA ATGGTAAAGTGGTGAATTTAGATCAAATAAGAGAAGATGGAAAGAAAAAATATTTGCCAAAAACGTATACGTTTGATGAT GGGGCAATTGTTTTAAAAGCTGGAGATAAAGTGACAGAAGAAAAAGTAAAACGTTTATATTGGGCGGCAAAAGAAGTAAA GGCACAATTCCATCGTACGGTTGAAAGTGATCAACCTTTAGAAAAAGGAAATCCTGATGATGTATTAACGATGGTTATTT ATAATAGCCCAGCAGAATATCAATTTAATCGTCAATTGTACGGTTATGAAACGAATAACGGTGGTCTTTATATAGAAGGA ACAGGTACGTTCTTTACTTATGAGCGTACGCCAGAAGAAAGTATTTATAGTTTAGAAGAATTATTCAGACACGAGTTCAC GCATTATTTACAAGGTAGATATGAAGTACCAGGGTTATGGGGACAAGGGAAAATATATGAAAATGAAAGATTATCTTGGT TTGAAGAAGGAAATGCTGAATTTTTTGCAGGGGCAACGAGAACGGATAATGTTGTACCGAGAAAGAGTATTATAGGAGGA TTATCTTCAAATCCAGCAGAGCGTTATACGGCAGAGCGAACGTTAAATGCAAAGTATGGAACGTGGGATTTCTATAATTA TTCCTTTGCTTTACAATCGTATATGTACAATAAGAGATATGATATGTTCGATAAAATACATGATCTTATTAGGAAAAATG ATGTAACAGCATATGATGCATATCGCTCTACATTAAGTAAAGATGCGAATTTAAATAAAGAATATCAGGACTATATGCAA ATGCTAGTTGATAATCGTGAGAAATATAATGTGCCATTAGTATCAGACGATTATTTAGCGAATCATGCATCGAAGCCAGT TTCAGATATTGCAGCAGAAATAACAGCGGAAGCAAAATTGAAAAATGTATCAGTGAAGAAAAATAAATCACAGTTCTTTA ATACATTCACACTACAAGGAACATATACAGGAACTGCAGCAAAAGGAGAATATGAAGATTGGAAAACAATTACACAAAAC GTTAATGATACGTTAAAACGTTTAAGTGCAAAGGAATGGACAGGCTATAAAACAGTAACAGCATACTTTGTAAATTATCG CGTGAATACATCAGGACAATTTGAATATGATGTTGTGTTCCACGGTATTAATACAGAAGAAGGTGCAGAGAATAAAGCAC CAGTTGCAGTTATAAACGGTCCGTATAGCGGAAATGTAAATGAAGCAATTTCATTTACAAGCGACGGATCAAAAGATGAA GACGGGAAAATTACTTCGTATAAATGGGAGTTTGGTGATGGAGCAGTAAGTAATGAGCAAAATCCAACTCACGTATATAC AAAAGAAGGAACGTATACGACGAAATTAACGGTAACAGACGATAAAGGAGCAACTAATACTGCTACAGCGACTGTAACTG TTCAAAAGAAAGAAGATAACAGTGTAGAAAAAGAACCGAATAACTCATTCCAAACAGCAAACAAATTGCAGTTAAATCAA GTTTTACGTGCTAGTTTAGGAAATGGCGATACAAGTGATTTCTTTGAAATTAATGTGGAGACTGCTAAAAACCTTCAAAT TAACGTAACGAATGAAAATAATATTGGAATGAACTGGGTACTTTATTCTGAAGCAGATTTAAATAATTATGTTACGTATG CTCAGCAACAAGGGAATAAACTAGTAGGTAGTTACTATACGTATCCAGGGAAGTATTATTTACATGTGTATCAGTATGGC GGGGGGACAGGGAATTATACGGTGGAAGTGAAGTAG
Upstream 100 bases:
>100_bases ACATGTGTGAACGTTACCTTATTTTTTCTCAATACAGGACAAAAAGTAAAAATAGTAAAATAATGGAGCGTGACACTAAA AATGTAAGGTAGGGGAGAGC
Downstream 100 bases:
>100_bases TAAAAGGGGAGTTGTCTTTTTCAATGAATGGAAAAAGGTCTGTACTTTAATGAAAAAACAACTGCAGGCAATGTCTCTAA TGATAATAACAAATTAAGTG
Product: putative microbial collagenase
Products: NA
Alternate protein names: 120 kDa collagenase [H]
Number of amino acids: Translated: 971; Mature: 971
Protein sequence:
>971_residues MKGYSKKMLVGVSFASLMLGSFQGVSLAEDTKGEQVSYRNVLKMEPVGVQLPVQELAHSSKVLESKSFEKRLQFADLSQR PPEVKKESKQLAVAKTYTIAELNQLSNQQLVDLLVTIDWEQITGLFQFNTDSLAFYQNDSRMQAIIDKLKQQGQAYTKDD SKGIETLVEVLRSGFYLGFYNSELSKLNERSYHDKCLPALKAMANNPNFKLGTLEQNRVVSSYGKLIGNASSDVETITSA AKIFKQYNDNFSTLVDNLSAGNAIYDIMQGVDYDIQSYLYDTRKAPKDTVWYQKIDSYINELSRFALMGTITAKNGWLIN NGIYYTGRLGMFHSTGTKGLQVVTDAMKMYPYLGEQYFVAAEQIATNYGGKDANGKVVNLDQIREDGKKKYLPKTYTFDD GAIVLKAGDKVTEEKVKRLYWAAKEVKAQFHRTVESDQPLEKGNPDDVLTMVIYNSPAEYQFNRQLYGYETNNGGLYIEG TGTFFTYERTPEESIYSLEELFRHEFTHYLQGRYEVPGLWGQGKIYENERLSWFEEGNAEFFAGATRTDNVVPRKSIIGG LSSNPAERYTAERTLNAKYGTWDFYNYSFALQSYMYNKRYDMFDKIHDLIRKNDVTAYDAYRSTLSKDANLNKEYQDYMQ MLVDNREKYNVPLVSDDYLANHASKPVSDIAAEITAEAKLKNVSVKKNKSQFFNTFTLQGTYTGTAAKGEYEDWKTITQN VNDTLKRLSAKEWTGYKTVTAYFVNYRVNTSGQFEYDVVFHGINTEEGAENKAPVAVINGPYSGNVNEAISFTSDGSKDE DGKITSYKWEFGDGAVSNEQNPTHVYTKEGTYTTKLTVTDDKGATNTATATVTVQKKEDNSVEKEPNNSFQTANKLQLNQ VLRASLGNGDTSDFFEINVETAKNLQINVTNENNIGMNWVLYSEADLNNYVTYAQQQGNKLVGSYYTYPGKYYLHVYQYG GGTGNYTVEVK
Sequences:
>Translated_971_residues MKGYSKKMLVGVSFASLMLGSFQGVSLAEDTKGEQVSYRNVLKMEPVGVQLPVQELAHSSKVLESKSFEKRLQFADLSQR PPEVKKESKQLAVAKTYTIAELNQLSNQQLVDLLVTIDWEQITGLFQFNTDSLAFYQNDSRMQAIIDKLKQQGQAYTKDD SKGIETLVEVLRSGFYLGFYNSELSKLNERSYHDKCLPALKAMANNPNFKLGTLEQNRVVSSYGKLIGNASSDVETITSA AKIFKQYNDNFSTLVDNLSAGNAIYDIMQGVDYDIQSYLYDTRKAPKDTVWYQKIDSYINELSRFALMGTITAKNGWLIN NGIYYTGRLGMFHSTGTKGLQVVTDAMKMYPYLGEQYFVAAEQIATNYGGKDANGKVVNLDQIREDGKKKYLPKTYTFDD GAIVLKAGDKVTEEKVKRLYWAAKEVKAQFHRTVESDQPLEKGNPDDVLTMVIYNSPAEYQFNRQLYGYETNNGGLYIEG TGTFFTYERTPEESIYSLEELFRHEFTHYLQGRYEVPGLWGQGKIYENERLSWFEEGNAEFFAGATRTDNVVPRKSIIGG LSSNPAERYTAERTLNAKYGTWDFYNYSFALQSYMYNKRYDMFDKIHDLIRKNDVTAYDAYRSTLSKDANLNKEYQDYMQ MLVDNREKYNVPLVSDDYLANHASKPVSDIAAEITAEAKLKNVSVKKNKSQFFNTFTLQGTYTGTAAKGEYEDWKTITQN VNDTLKRLSAKEWTGYKTVTAYFVNYRVNTSGQFEYDVVFHGINTEEGAENKAPVAVINGPYSGNVNEAISFTSDGSKDE DGKITSYKWEFGDGAVSNEQNPTHVYTKEGTYTTKLTVTDDKGATNTATATVTVQKKEDNSVEKEPNNSFQTANKLQLNQ VLRASLGNGDTSDFFEINVETAKNLQINVTNENNIGMNWVLYSEADLNNYVTYAQQQGNKLVGSYYTYPGKYYLHVYQYG GGTGNYTVEVK >Mature_971_residues MKGYSKKMLVGVSFASLMLGSFQGVSLAEDTKGEQVSYRNVLKMEPVGVQLPVQELAHSSKVLESKSFEKRLQFADLSQR PPEVKKESKQLAVAKTYTIAELNQLSNQQLVDLLVTIDWEQITGLFQFNTDSLAFYQNDSRMQAIIDKLKQQGQAYTKDD SKGIETLVEVLRSGFYLGFYNSELSKLNERSYHDKCLPALKAMANNPNFKLGTLEQNRVVSSYGKLIGNASSDVETITSA AKIFKQYNDNFSTLVDNLSAGNAIYDIMQGVDYDIQSYLYDTRKAPKDTVWYQKIDSYINELSRFALMGTITAKNGWLIN NGIYYTGRLGMFHSTGTKGLQVVTDAMKMYPYLGEQYFVAAEQIATNYGGKDANGKVVNLDQIREDGKKKYLPKTYTFDD GAIVLKAGDKVTEEKVKRLYWAAKEVKAQFHRTVESDQPLEKGNPDDVLTMVIYNSPAEYQFNRQLYGYETNNGGLYIEG TGTFFTYERTPEESIYSLEELFRHEFTHYLQGRYEVPGLWGQGKIYENERLSWFEEGNAEFFAGATRTDNVVPRKSIIGG LSSNPAERYTAERTLNAKYGTWDFYNYSFALQSYMYNKRYDMFDKIHDLIRKNDVTAYDAYRSTLSKDANLNKEYQDYMQ MLVDNREKYNVPLVSDDYLANHASKPVSDIAAEITAEAKLKNVSVKKNKSQFFNTFTLQGTYTGTAAKGEYEDWKTITQN VNDTLKRLSAKEWTGYKTVTAYFVNYRVNTSGQFEYDVVFHGINTEEGAENKAPVAVINGPYSGNVNEAISFTSDGSKDE DGKITSYKWEFGDGAVSNEQNPTHVYTKEGTYTTKLTVTDDKGATNTATATVTVQKKEDNSVEKEPNNSFQTANKLQLNQ VLRASLGNGDTSDFFEINVETAKNLQINVTNENNIGMNWVLYSEADLNNYVTYAQQQGNKLVGSYYTYPGKYYLHVYQYG GGTGNYTVEVK
Specific function: Unknown
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PKD domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013510 - InterPro: IPR007280 - InterPro: IPR002169 - InterPro: IPR013661 - InterPro: IPR022409 - InterPro: IPR000601 [H]
Pfam domain/function: PF01752 Peptidase_M9; PF08453 Peptidase_M9_N; PF00801 PKD; PF04151 PPC [H]
EC number: =3.4.24.3 [H]
Molecular weight: Translated: 109911; Mature: 109911
Theoretical pI: Translated: 5.40; Mature: 5.40
Prosite motif: PS50093 PKD ; PS00142 ZINC_PROTEASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 1.9 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKGYSKKMLVGVSFASLMLGSFQGVSLAEDTKGEQVSYRNVLKMEPVGVQLPVQELAHSS CCCCCCEEEEEHHHHHHHHHCCCCCEECCCCCCCCCHHCCCEEECCCCCCCCHHHHHHHH KVLESKSFEKRLQFADLSQRPPEVKKESKQLAVAKTYTIAELNQLSNQQLVDLLVTIDWE HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEEHHHHHHHCCCHHEEEEEEEECHH QITGLFQFNTDSLAFYQNDSRMQAIIDKLKQQGQAYTKDDSKGIETLVEVLRSGFYLGFY HHCEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEE NSELSKLNERSYHDKCLPALKAMANNPNFKLGTLEQNRVVSSYGKLIGNASSDVETITSA CHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHH AKIFKQYNDNFSTLVDNLSAGNAIYDIMQGVDYDIQSYLYDTRKAPKDTVWYQKIDSYIN HHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH ELSRFALMGTITAKNGWLINNGIYYTGRLGMFHSTGTKGLQVVTDAMKMYPYLGEQYFVA HHHHHEEEEEEEECCCEEEECCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHH AEQIATNYGGKDANGKVVNLDQIREDGKKKYLPKTYTFDDGAIVLKAGDKVTEEKVKRLY HHHHHHCCCCCCCCCEEEEHHHHHHCHHHCCCCCEEEECCCEEEEECCCCHHHHHHHHHH WAAKEVKAQFHRTVESDQPLEKGNPDDVLTMVIYNSPAEYQFNRQLYGYETNNGGLYIEG HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEECCEEEEEEECCCEEEEEE TGTFFTYERTPEESIYSLEELFRHEFTHYLQGRYEVPGLWGQGKIYENERLSWFEEGNAE CCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEECCCCCCEEECCCCE FFAGATRTDNVVPRKSIIGGLSSNPAERYTAERTLNAKYGTWDFYNYSFALQSYMYNKRY EEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCH DMFDKIHDLIRKNDVTAYDAYRSTLSKDANLNKEYQDYMQMLVDNREKYNVPLVSDDYLA HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEECCHHHH NHASKPVSDIAAEITAEAKLKNVSVKKNKSQFFNTFTLQGTYTGTAAKGEYEDWKTITQN HCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHEEEEEEEEEEECCCCCCCCHHHHHHHHH VNDTLKRLSAKEWTGYKTVTAYFVNYRVNTSGQFEYDVVFHGINTEEGAENKAPVAVING HHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEEEECC PYSGNVNEAISFTSDGSKDEDGKITSYKWEFGDGAVSNEQNPTHVYTKEGTYTTKLTVTD CCCCCHHHEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEEEEEC DKGATNTATATVTVQKKEDNSVEKEPNNSFQTANKLQLNQVLRASLGNGDTSDFFEINVE CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEE TAKNLQINVTNENNIGMNWVLYSEADLNNYVTYAQQQGNKLVGSYYTYPGKYYLHVYQYG ECCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEEEECCCEEEEEEEEEC GGTGNYTVEVK CCCCCEEEEEC >Mature Secondary Structure MKGYSKKMLVGVSFASLMLGSFQGVSLAEDTKGEQVSYRNVLKMEPVGVQLPVQELAHSS CCCCCCEEEEEHHHHHHHHHCCCCCEECCCCCCCCCHHCCCEEECCCCCCCCHHHHHHHH KVLESKSFEKRLQFADLSQRPPEVKKESKQLAVAKTYTIAELNQLSNQQLVDLLVTIDWE HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEEEHHHHHHHCCCHHEEEEEEEECHH QITGLFQFNTDSLAFYQNDSRMQAIIDKLKQQGQAYTKDDSKGIETLVEVLRSGFYLGFY HHCEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEE NSELSKLNERSYHDKCLPALKAMANNPNFKLGTLEQNRVVSSYGKLIGNASSDVETITSA CHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHH AKIFKQYNDNFSTLVDNLSAGNAIYDIMQGVDYDIQSYLYDTRKAPKDTVWYQKIDSYIN HHHHHHHCCCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH ELSRFALMGTITAKNGWLINNGIYYTGRLGMFHSTGTKGLQVVTDAMKMYPYLGEQYFVA HHHHHEEEEEEEECCCEEEECCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHH AEQIATNYGGKDANGKVVNLDQIREDGKKKYLPKTYTFDDGAIVLKAGDKVTEEKVKRLY HHHHHHCCCCCCCCCEEEEHHHHHHCHHHCCCCCEEEECCCEEEEECCCCHHHHHHHHHH WAAKEVKAQFHRTVESDQPLEKGNPDDVLTMVIYNSPAEYQFNRQLYGYETNNGGLYIEG HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEECCEEEEEEECCCEEEEEE TGTFFTYERTPEESIYSLEELFRHEFTHYLQGRYEVPGLWGQGKIYENERLSWFEEGNAE CCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEECCCCCCEEECCCCE FFAGATRTDNVVPRKSIIGGLSSNPAERYTAERTLNAKYGTWDFYNYSFALQSYMYNKRY EEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCH DMFDKIHDLIRKNDVTAYDAYRSTLSKDANLNKEYQDYMQMLVDNREKYNVPLVSDDYLA HHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEECCHHHH NHASKPVSDIAAEITAEAKLKNVSVKKNKSQFFNTFTLQGTYTGTAAKGEYEDWKTITQN HCCCCCHHHHHHHHHHHHHHCCCEEECCCHHHEEEEEEEEEEECCCCCCCCHHHHHHHHH VNDTLKRLSAKEWTGYKTVTAYFVNYRVNTSGQFEYDVVFHGINTEEGAENKAPVAVING HHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEEEECC PYSGNVNEAISFTSDGSKDEDGKITSYKWEFGDGAVSNEQNPTHVYTKEGTYTTKLTVTD CCCCCHHHEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEEEEEC DKGATNTATATVTVQKKEDNSVEKEPNNSFQTANKLQLNQVLRASLGNGDTSDFFEINVE CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEE TAKNLQINVTNENNIGMNWVLYSEADLNNYVTYAQQQGNKLVGSYYTYPGKYYLHVYQYG ECCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEEEEECCCEEEEEEEEEC GGTGNYTVEVK CCCCCEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8282691; 11792842 [H]