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The map label for this gene is amyS [H]

Identifier: 218233306

GI number: 218233306

Start: 3366363

End: 3367904

Strand: Reverse

Name: amyS [H]

Synonym: BCB4264_A3487

Alternate gene names: 218233306

Gene position: 3367904-3366363 (Counterclockwise)

Preceding gene: 218233965

Following gene: 218232809

Centisome position: 62.15

GC content: 37.35

Gene sequence:

>1542_bases
ATGTTTAAAAGAATAACAATAGTCGGATTGTCAGTTGTTATGTTTTTACCTAGTATATATGAGGGGAGTAAGGCATATGC
AGACACAGTTAACAATGGAACGTTAATGCAGTATTTTGAGTGGTATTCTCCGAATGATGGGAATCATTGGAATCGTTTGC
GTACTGATGCTGAAAATTTAGCGGAAAAAGGAATTACATCTGTTTGGATACCACCTGCATATAAAGGAACTACGCAAAAT
GACGTAGGATATGGAGCATATGATTTATATGATCTGGGGGAATTCAATCAAAAGGGCACAGTGCGGACGAAATATGGGAC
GAAAGCACAATTGAAATCTGCAATTGACGCTTTACATAAGAAAAACATCGATGTATACGGTGATGTAGTTATGAATCATA
AAGGTGGGGCTGATTATACAGAAACTGTCACAGCAGTTGAGGTAGACCCGAGCAATCGAAATAATGAAGTATCAGGTGAT
TATGAAATTAGTGCGTGGACGGGATTTAACTTTCCAGGGCGTGGAGATTCTTATTCTAATTTCAAATGGAAATGGTATCA
TTTTGACGGAACGGATTGGGATGAAGGAAGGAAATTAAACCGAATTTATAAATTTAGGGGCATAGGTAAAGCATGGGACT
GGGAAGTATCTAGCGAGAATGGGAATTATGATTATTTGATGTATGCGGATCTTGATTTTGATCATCCAGATGTTGCGAAT
GAAATGAAAAAATGGGGAACGTGGTATGCGAATGAATTAAATTTAGATGGTTTTCGTTTAGATGCTGTTAAACATATTGA
TCATGAATATTTGCGTGATTGGGTAAATCACGTTAGACAGCAAACAGGGAAAGAAATGTTTACAGTAGCTGAATATTGGC
AAAATGATATCCAGACTTTAAATAATTATTTAGCGAAGGTTAATTATAATCAATCTGTGTTCGATGCACCACTTCATTAT
AATTTTCATTATGCTTCAACAGGAAATGGAAATTATGATATGAGAAATATTTTAAAAGGAACGGTAGTTGCGAATCATCC
TACACTTGCGGTTACTCTAGTTGAAAATCATGATTCACAGCCTGGTCAGTCATTGGAATCTGTAGTGAGCCCTTGGTTCA
AACCGCTGGCATATGCATTTATTTTAACGCGTGCAGAGGGGTATCCTTCTGTTTTCTATGGTGATTACTATGGTACAAAA
GGAAATAGTAACTATGAAATTCCAGCGTTAAAGGACAAAATTGATCCGATTTTGACGGCACGAAAAAACTTTGCATATGG
TACGCAACGTGATTATTTTGATCATCCAGATGTGATTGGCTGGACAAGAGAAGGTGATAGTGTACATGCTAATTCTGGTT
TAGCAACATTAATCTCTGATGGACCAGGAGGGGCGAAGTGGATGGATGTTGGAAAGAATAACGCAGGGGAAGTATGGTAT
GATATTACGGGTAATCAAACAAATACTGTAACAATTAATAAGGACGGATGGGGGCAGTTCCAAGTAAGTGGAGGATCAGT
TTCCATATATGTTCAACAATAA

Upstream 100 bases:

>100_bases
TGGGAGAGTGAGAGATTGCTACTTTACTAACGGTGGGACGAATATTATAATAATTCTGATAAACTATGCAAACGCTTTCT
TGGAAAGGGATGGTGTACAG

Downstream 100 bases:

>100_bases
TTTGGTGATGAAAAAGAAGAGGTAGACTGAAATATAAGTTTTCCTCTTCCTTTTTACATTCTGTATAGTTATAAAAGTAT
GACACCTTTACTTCTCACGT

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase; BLA [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MFKRITIVGLSVVMFLPSIYEGSKAYADTVNNGTLMQYFEWYSPNDGNHWNRLRTDAENLAEKGITSVWIPPAYKGTTQN
DVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIDALHKKNIDVYGDVVMNHKGGADYTETVTAVEVDPSNRNNEVSGD
YEISAWTGFNFPGRGDSYSNFKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
EMKKWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTGKEMFTVAEYWQNDIQTLNNYLAKVNYNQSVFDAPLHY
NFHYASTGNGNYDMRNILKGTVVANHPTLAVTLVENHDSQPGQSLESVVSPWFKPLAYAFILTRAEGYPSVFYGDYYGTK
GNSNYEIPALKDKIDPILTARKNFAYGTQRDYFDHPDVIGWTREGDSVHANSGLATLISDGPGGAKWMDVGKNNAGEVWY
DITGNQTNTVTINKDGWGQFQVSGGSVSIYVQQ

Sequences:

>Translated_513_residues
MFKRITIVGLSVVMFLPSIYEGSKAYADTVNNGTLMQYFEWYSPNDGNHWNRLRTDAENLAEKGITSVWIPPAYKGTTQN
DVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIDALHKKNIDVYGDVVMNHKGGADYTETVTAVEVDPSNRNNEVSGD
YEISAWTGFNFPGRGDSYSNFKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
EMKKWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTGKEMFTVAEYWQNDIQTLNNYLAKVNYNQSVFDAPLHY
NFHYASTGNGNYDMRNILKGTVVANHPTLAVTLVENHDSQPGQSLESVVSPWFKPLAYAFILTRAEGYPSVFYGDYYGTK
GNSNYEIPALKDKIDPILTARKNFAYGTQRDYFDHPDVIGWTREGDSVHANSGLATLISDGPGGAKWMDVGKNNAGEVWY
DITGNQTNTVTINKDGWGQFQVSGGSVSIYVQQ
>Mature_513_residues
MFKRITIVGLSVVMFLPSIYEGSKAYADTVNNGTLMQYFEWYSPNDGNHWNRLRTDAENLAEKGITSVWIPPAYKGTTQN
DVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIDALHKKNIDVYGDVVMNHKGGADYTETVTAVEVDPSNRNNEVSGD
YEISAWTGFNFPGRGDSYSNFKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
EMKKWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTGKEMFTVAEYWQNDIQTLNNYLAKVNYNQSVFDAPLHY
NFHYASTGNGNYDMRNILKGTVVANHPTLAVTLVENHDSQPGQSLESVVSPWFKPLAYAFILTRAEGYPSVFYGDYYGTK
GNSNYEIPALKDKIDPILTARKNFAYGTQRDYFDHPDVIGWTREGDSVHANSGLATLISDGPGGAKWMDVGKNNAGEVWY
DITGNQTNTVTINKDGWGQFQVSGGSVSIYVQQ

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=492, Percent_Identity=42.2764227642276, Blast_Score=395, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 58310; Mature: 58310

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRITIVGLSVVMFLPSIYEGSKAYADTVNNGTLMQYFEWYSPNDGNHWNRLRTDAENL
CCCEEEEHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCHHHH
AEKGITSVWIPPAYKGTTQNDVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIDALHK
HHCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHH
KNIDVYGDVVMNHKGGADYTETVTAVEVDPSNRNNEVSGDYEISAWTGFNFPGRGDSYSN
CCCCEEEEEEEECCCCCCHHHEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
FKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
CEEEEEECCCCCHHCCCHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEEECCCCCCHHHH
EMKKWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTGKEMFTVAEYWQNDIQTL
HHHHHCCEECCEECCCCEEEHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
NNYLAKVNYNQSVFDAPLHYNFHYASTGNGNYDMRNILKGTVVANHPTLAVTLVENHDSQ
HHHHEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCEEECCCEEEEEEEECCCCC
PGQSLESVVSPWFKPLAYAFILTRAEGYPSVFYGDYYGTKGNSNYEIPALKDKIDPILTA
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCCCCCEECCCHHHHHHHHHHH
RKNFAYGTQRDYFDHPDVIGWTREGDSVHANSGLATLISDGPGGAKWMDVGKNNAGEVWY
HCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEEECCCCCCEEEECCCCCCCCEEE
DITGNQTNTVTINKDGWGQFQVSGGSVSIYVQQ
EECCCCEEEEEEECCCCCEEEEECCEEEEEEEC
>Mature Secondary Structure
MFKRITIVGLSVVMFLPSIYEGSKAYADTVNNGTLMQYFEWYSPNDGNHWNRLRTDAENL
CCCEEEEHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCHHHH
AEKGITSVWIPPAYKGTTQNDVGYGAYDLYDLGEFNQKGTVRTKYGTKAQLKSAIDALHK
HHCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHH
KNIDVYGDVVMNHKGGADYTETVTAVEVDPSNRNNEVSGDYEISAWTGFNFPGRGDSYSN
CCCCEEEEEEEECCCCCCHHHEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
FKWKWYHFDGTDWDEGRKLNRIYKFRGIGKAWDWEVSSENGNYDYLMYADLDFDHPDVAN
CEEEEEECCCCCHHCCCHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEEECCCCCCHHHH
EMKKWGTWYANELNLDGFRLDAVKHIDHEYLRDWVNHVRQQTGKEMFTVAEYWQNDIQTL
HHHHHCCEECCEECCCCEEEHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
NNYLAKVNYNQSVFDAPLHYNFHYASTGNGNYDMRNILKGTVVANHPTLAVTLVENHDSQ
HHHHEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCEEECCCEEEEEEEECCCCC
PGQSLESVVSPWFKPLAYAFILTRAEGYPSVFYGDYYGTKGNSNYEIPALKDKIDPILTA
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEECCCCCCCCCCCEECCCHHHHHHHHHHH
RKNFAYGTQRDYFDHPDVIGWTREGDSVHANSGLATLISDGPGGAKWMDVGKNNAGEVWY
HCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEEECCCCCCEEEECCCCCCCCEEE
DITGNQTNTVTINKDGWGQFQVSGGSVSIYVQQ
EECCCCEEEEEEECCCCCEEEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2418011; 3009417; 14632998; 6334606; 6609154; 2265757; 2540150; 6172418; 11997021; 2394736; 8771184; 10966804; 12736372; 12915728; 7877175; 9551551; 10924103; 12540849 [H]