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The map label for this gene is aacA-aphD [H]

Identifier: 218233253

GI number: 218233253

Start: 1377457

End: 1378359

Strand: Reverse

Name: aacA-aphD [H]

Synonym: BCB4264_A1431

Alternate gene names: 218233253

Gene position: 1378359-1377457 (Counterclockwise)

Preceding gene: 218233087

Following gene: 218232587

Centisome position: 25.44

GC content: 34.0

Gene sequence:

>903_bases
ATGGACTCTTACAAACATTATATAAAAGAAGCTTTGCCTAATCTTTCTATACATTCATATAAACAAAATGAAGAAGGATG
GGATAATGTAGCAGTTATAGTAAATGATGAGCTACTGTTTCGTTTCCCGCGAAAACAGGAATATGCGATGCGAATTCCGT
TAGAAAAAGAACTATGCGCAATTCTCACTCAATCACTACAAGAAATCGAAGTTCCACAATATCACCTAATTTATAAAAAT
GAATCTGATGAGGTTCCTCTTTGTAGTTACTATACTCTCATTCATGGTGAACCATTAAAAACAGAAACAGTTGCCACGCT
GGAGGATACGGAGCTGAAAGCAATTATTACACAATTAGCTACTTTCCTTGCGGTACTACACAGTATTCCTTTACACCAAG
TTACAACGCTATGTTTCCCTATTGAAAAAACATGTACCTACTGGAAAGAGCTACAAACAAAATTAAACCAATATCTCACT
ACTAGCCTTACTTCATTACAGAAACTAGCCTTAAATCGCTTATTTGAAAATTTCTTTGCATGTTTAGCTACATCCACTTT
TCAAAACACAATCATTCACGCTGATTTTACTCATCATCACATTTTATTTAACAAGCATAACAAAAGCATTTCAGGTGTTA
TCGATTTTGGTGATGCGCAAATTGGCGATCCTGCTTTTGATTTTGCAGGACTATATTATGATTTCGGAGATGAGTTTACT
ACATCTGTATATGAACAGTACAGTACACTTATTTCTCATCATGATCCATTACTCATTCATCGTATTACTACCTTTTATCA
ATACAGTCCTTTACTACATAATATTATTTATAATTTTGAGTCAGAGAATGAACTCGAATTCATTACAGGCACAGAACAGC
TAAAAACGATATTACAGGGATGA

Upstream 100 bases:

>100_bases
CTCCCGCCCTTCTTCATAATAATAGATATATTTCAACATATAGAAAGAAAATTCTGATATCATAAAATAGAATAACTTAA
CTCAGAAACGAGGAACAATT

Downstream 100 bases:

>100_bases
GATTACATTCTCTCCCTATATACTCTCTCCTCATACATCATTAACTTTTTATCCATTTCAACAATAACTTTATACAATTG
AAACGCCTGTAAAATTTGCA

Product: aminoglycoside phophotransferase family protein

Products: NA

Alternate protein names: 6'-aminoglycoside N-acetyltransferase; AAC(6'); 2''-aminoglycoside phosphotransferase; APH(2'') [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDSYKHYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCAILTQSLQEIEVPQYHLIYKN
ESDEVPLCSYYTLIHGEPLKTETVATLEDTELKAIITQLATFLAVLHSIPLHQVTTLCFPIEKTCTYWKELQTKLNQYLT
TSLTSLQKLALNRLFENFFACLATSTFQNTIIHADFTHHHILFNKHNKSISGVIDFGDAQIGDPAFDFAGLYYDFGDEFT
TSVYEQYSTLISHHDPLLIHRITTFYQYSPLLHNIIYNFESENELEFITGTEQLKTILQG

Sequences:

>Translated_300_residues
MDSYKHYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCAILTQSLQEIEVPQYHLIYKN
ESDEVPLCSYYTLIHGEPLKTETVATLEDTELKAIITQLATFLAVLHSIPLHQVTTLCFPIEKTCTYWKELQTKLNQYLT
TSLTSLQKLALNRLFENFFACLATSTFQNTIIHADFTHHHILFNKHNKSISGVIDFGDAQIGDPAFDFAGLYYDFGDEFT
TSVYEQYSTLISHHDPLLIHRITTFYQYSPLLHNIIYNFESENELEFITGTEQLKTILQG
>Mature_300_residues
MDSYKHYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCAILTQSLQEIEVPQYHLIYKN
ESDEVPLCSYYTLIHGEPLKTETVATLEDTELKAIITQLATFLAVLHSIPLHQVTTLCFPIEKTCTYWKELQTKLNQYLT
TSLTSLQKLALNRLFENFFACLATSTFQNTIIHADFTHHHILFNKHNKSISGVIDFGDAQIGDPAFDFAGLYYDFGDEFT
TSVYEQYSTLISHHDPLLIHRITTFYQYSPLLHNIIYNFESENELEFITGTEQLKTILQG

Specific function: Resistance to gentamicin, tobramycin, and kanamycin. Tobramycin and kanamycin resistance is due to the ACC activity, specified by N-terminal region, and the gentamicin resistance is due to the APH activity encoded by the C-terminal region of the protein [

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF01636 APH [H]

EC number: NA

Molecular weight: Translated: 34804; Mature: 34804

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSYKHYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCA
CCHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECHHEEECCCCCCCEEECCCHHHHHH
ILTQSLQEIEVPQYHLIYKNESDEVPLCSYYTLIHGEPLKTETVATLEDTELKAIITQLA
HHHHHHHHCCCCCEEEEEECCCCCCCHHHEEHEECCCCCCCCEEEECCHHHHHHHHHHHH
TFLAVLHSIPLHQVTTLCFPIEKTCTYWKELQTKLNQYLTTSLTSLQKLALNRLFENFFA
HHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLATSTFQNTIIHADFTHHHILFNKHNKSISGVIDFGDAQIGDPAFDFAGLYYDFGDEFT
HHHHHHHHHCEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHH
TSVYEQYSTLISHHDPLLIHRITTFYQYSPLLHNIIYNFESENELEFITGTEQLKTILQG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCC
>Mature Secondary Structure
MDSYKHYIKEALPNLSIHSYKQNEEGWDNVAVIVNDELLFRFPRKQEYAMRIPLEKELCA
CCHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECHHEEECCCCCCCEEECCCHHHHHH
ILTQSLQEIEVPQYHLIYKNESDEVPLCSYYTLIHGEPLKTETVATLEDTELKAIITQLA
HHHHHHHHCCCCCEEEEEECCCCCCCHHHEEHEECCCCCCCCEEEECCHHHHHHHHHHHH
TFLAVLHSIPLHQVTTLCFPIEKTCTYWKELQTKLNQYLTTSLTSLQKLALNRLFENFFA
HHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLATSTFQNTIIHADFTHHHILFNKHNKSISGVIDFGDAQIGDPAFDFAGLYYDFGDEFT
HHHHHHHHHCEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHH
TSVYEQYSTLISHHDPLLIHRITTFYQYSPLLHNIIYNFESENELEFITGTEQLKTILQG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3015884; 12663927 [H]