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The map label for this gene is citS [H]

Identifier: 218233196

GI number: 218233196

Start: 575084

End: 576685

Strand: Direct

Name: citS [H]

Synonym: BCB4264_A0595

Alternate gene names: 218233196

Gene position: 575084-576685 (Clockwise)

Preceding gene: 218232180

Following gene: 218230963

Centisome position: 10.61

GC content: 34.02

Gene sequence:

>1602_bases
TTGAAATTAAAAAAATTAAAACTCCAACCGAGAATTACATTAACTATTAGTACACTTATTTTAGTTGTACTTATGCTAAC
GAGTTATTTATTTTATTACATATTATCTGAAACGGTAGAAGAGCAAATTGGCAAAAGGGCTTTGCATGTTGCGAAAACAG
TTGCTGCTATACCTGAAATAAAGGAAGCTTTTCAAAAAGAAAACCCAGCTTCTATTATTCAGCCGATTGCAGAAAGAATT
CGAATGGATACAGAGGCTGACTTCATTGTAGTTGGAAATAAAGAGGGGATTCGATATGCACATCCTGAGCGAGATAAAAT
AGGAGAAGCGATGATTGGTGGAGATAACAAAGGCGTTTTATTAGAAGGAAAATCATATGTTTCGAAAGCAACTGGATCAT
TAGGCCCTTCATTACGCGGAAAAGTTCCAATCCGTAATCAGGAAAATGAAATCATTGGTGTGGTGTCTGTTGGATTTTCA
ATGGATGATATTCATGGAGCGGTAGAGGTGTATGGAAAGCGTGTATTTTGGATTACAATAATAGGGCTATTAATTGGAGT
AATCGGCTCTATATATTTAGCGGCAAGCATAAAAAGAATGATGTTCGGTATGGAACCAGAAGAAATTTCATCTTTATATG
AAGAGCATAGTACAGTGATTCAATCTGTACGTGAAGGGATTATTGTAATCGATAAAAATGGCATGATTAGTTTAGTCAAT
CAAGCAGCTTATGATATTCTTTCGCTAGATAAACAACAAAATATTATTGGGGAATTTATTTTAAATGTAATTCCTAATTC
AACGATACTAGATGTACTTCAAACTGGTGAAGAACAGTTTGATCGCCAATTAAATATAAAAGGACAGGCTGTTATTGCGA
ACCGTCTACCTATTAAAGTAAAGAATAAAGTAACTGGCGTTGTATCGAGCTTACGTTTGAAATCTGAAATGGATCAGTTA
ACAGCAGAATTATCTCAGACGAAGCAATATACAGAGGCATTACGAGCGCAAGCGCACGAATATAACAATTTATTGTATAC
GCTTTCAGGTCTTATCCAATTAGAATCATATGAAGATGCTTTAGAGCTTATTCATAAGGAAACAGCGGTATATCAAGATT
TTGTTCAATTTATTATGAAGCGGATTCAAAATCCATGGCTAGGTGGAATTTTAATTGGATTTTATAATAGAGCACGAGAG
TTGAAGATTGATTTTATGCTAGATAGAGAAAGTGGTTTAGACAAATTAAGTCCACATATTGAGAGTAATTATGTCGTTTC
TATTTTAGGAAATTTAATTACGAATGCATTTGAAGCAATTGAAAAAAATGAAGAGAATGATAAGAGAGTAAGGATGTTTG
TAACTGATATTGGAGAAGAAATATTAATTGAAGTGGAAGATTCAGGACAAGGGATTCATGATGAAATAATTACTAGCATA
TTCTATAAAGGCTTTTCGACGAAAGAAGGAGGAGAGCGAGGATATGGATTAGCAAAAGTGAAAGAGTTAGTAGAAGATTT
AAATGGAAGTATTGCAATTGAAAAAGGGGATTTAGGTGGTGCGTTATTTATTATTGCACTACCGAAAGAGAGAGGCGAAT
AG

Upstream 100 bases:

>100_bases
AAAAAGAAAGAAATATAGAGTAACCGTGTTATGATTAAGTATAATTAAAGAGACTGTATATTCTTTAAATTCTATCTTTA
ATCATGTTAGGGGAATGGTT

Downstream 100 bases:

>100_bases
TAATGAGTGAGGAGATTGAAGTATTAATTGTAGAAGATGATATTCGGATTGCTGACATTCATCGTCGTTTTACTGAAAAA
ATTGAGGGATTTAAAGTAAT

Product: sensor protein CitS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKLKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQKENPASIIQPIAERI
RMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFS
MDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAASIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDKNGMISLVN
QAAYDILSLDKQQNIIGEFILNVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMDQL
TAELSQTKQYTEALRAQAHEYNNLLYTLSGLIQLESYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARE
LKIDFMLDRESGLDKLSPHIESNYVVSILGNLITNAFEAIEKNEENDKRVRMFVTDIGEEILIEVEDSGQGIHDEIITSI
FYKGFSTKEGGERGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGE

Sequences:

>Translated_533_residues
MKLKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQKENPASIIQPIAERI
RMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFS
MDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAASIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDKNGMISLVN
QAAYDILSLDKQQNIIGEFILNVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMDQL
TAELSQTKQYTEALRAQAHEYNNLLYTLSGLIQLESYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARE
LKIDFMLDRESGLDKLSPHIESNYVVSILGNLITNAFEAIEKNEENDKRVRMFVTDIGEEILIEVEDSGQGIHDEIITSI
FYKGFSTKEGGERGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGE
>Mature_533_residues
MKLKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEIKEAFQKENPASIIQPIAERI
RMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVLLEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFS
MDDIHGAVEVYGKRVFWITIIGLLIGVIGSIYLAASIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDKNGMISLVN
QAAYDILSLDKQQNIIGEFILNVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKVKNKVTGVVSSLRLKSEMDQL
TAELSQTKQYTEALRAQAHEYNNLLYTLSGLIQLESYEDALELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARE
LKIDFMLDRESGLDKLSPHIESNYVVSILGNLITNAFEAIEKNEENDKRVRMFVTDIGEEILIEVEDSGQGIHDEIITSI
FYKGFSTKEGGERGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGE

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=533, Percent_Identity=32.2701688555347, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1786837, Length=535, Percent_Identity=31.7757009345794, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1788549, Length=342, Percent_Identity=23.3918128654971, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59497; Mature: 59497

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEI
CCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAFQKENPASIIQPIAERIRMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVL
HHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHCCHHCCCCCCCEE
LEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWITI
EECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHH
IGLLIGVIGSIYLAASIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDKNGMISLVN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
QAAYDILSLDKQQNIIGEFILNVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKV
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCEEE
KNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQAHEYNNLLYTLSGLIQLESYEDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIDFMLDRESGLDKLSPHI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCC
ESNYVVSILGNLITNAFEAIEKNEENDKRVRMFVTDIGEEILIEVEDSGQGIHDEIITSI
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHEEHHHHHCCCEEEEEECCCCCCHHHHHHHHH
FYKGFSTKEGGERGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGE
HHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKLKKLKLQPRITLTISTLILVVLMLTSYLFYYILSETVEEQIGKRALHVAKTVAAIPEI
CCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEAFQKENPASIIQPIAERIRMDTEADFIVVGNKEGIRYAHPERDKIGEAMIGGDNKGVL
HHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHCCHHCCCCCCCEE
LEGKSYVSKATGSLGPSLRGKVPIRNQENEIIGVVSVGFSMDDIHGAVEVYGKRVFWITI
EECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHH
IGLLIGVIGSIYLAASIKRMMFGMEPEEISSLYEEHSTVIQSVREGIIVIDKNGMISLVN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
QAAYDILSLDKQQNIIGEFILNVIPNSTILDVLQTGEEQFDRQLNIKGQAVIANRLPIKV
HHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEEECCCCEEE
KNKVTGVVSSLRLKSEMDQLTAELSQTKQYTEALRAQAHEYNNLLYTLSGLIQLESYEDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELIHKETAVYQDFVQFIMKRIQNPWLGGILIGFYNRARELKIDFMLDRESGLDKLSPHI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCHHHCCCC
ESNYVVSILGNLITNAFEAIEKNEENDKRVRMFVTDIGEEILIEVEDSGQGIHDEIITSI
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHEEHHHHHCCCEEEEEECCCCCCHHHHHHHHH
FYKGFSTKEGGERGYGLAKVKELVEDLNGSIAIEKGDLGGALFIIALPKERGE
HHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]