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The map label for this gene is nadE [H]

Identifier: 218233156

GI number: 218233156

Start: 1911940

End: 1912758

Strand: Reverse

Name: nadE [H]

Synonym: BCB4264_A2000

Alternate gene names: 218233156

Gene position: 1912758-1911940 (Counterclockwise)

Preceding gene: 218234608

Following gene: 218235605

Centisome position: 35.3

GC content: 37.36

Gene sequence:

>819_bases
ATGACATTACAAGAACAGATTATGAAAGCATTACATGTTCAGCCTGTAATTGATCCAAAAGTTGAAATTCGTAAACGAGT
TGATTTCTTAAAGGATTATGTAAAAAAAACAGGTGCAAAAGGATTTGTACTTGGTATTAGCGGCGGACAAGACTCTACAT
TAGCAGGACGCTTAGCACAACTTGCAGTTGAAGAAATTCGTAACGAAGGTGGTAATGCAACGTTTATCGCCGTGCGTCTT
CCTTATAAAGTACAAAAAGATGAAGATGATGCACAATTAGCATTACAATTCATTCAAGCAGATCAATCTGTTGCATTTGA
TATTGCTTCAACTGTTGATGCTTTTTCAAATCAATATGAAAACTTATTGGGTGAATCACTAACAGATTTCAATAAAGGTA
ACGTGAAAGCACGTATCCGTATGGTTACTCAATATGCAATTGGTGGACAAAAAGGGCTACTTGTTATCGGAACAGATCAC
GCTGCAGAAGCTGTAACAGGGTTCTTTACAAAATTCGGAGATGGTGGTGCAGATTTATTACCATTAACGGGATTAACGAA
GCGCCAAGGACGTGCATTATTACAAGAGTTAGGCGCAGACGAGCGACTGTACTTAAAAATGCCAACAGCTGATTTATTAG
ATGAAAAACCAGGTCAAGCTGATGAAACAGAGTTAGGTATTACTTATGATCAATTAGATGATTATTTAGAAGGTAAAGCA
GTTCCAGCTGACGTTGCAGAGAAAATTGAAAAACGTTACACAGTGAGTGAGCATAAAAGACAAGTACCAGCGTCAATGTT
TGATGATTGGTGGAAATAA

Upstream 100 bases:

>100_bases
TGTATATTCGTTAGTTTTGCGAAATAATAAAGGATAGAAGTATGTACCATTATACATATAAACTTATTTACCGCTTTAAT
ATGAATAGGAGGAAAACATA

Downstream 100 bases:

>100_bases
ATTAAACATAAAAAAGAAGCTAATCTTCATTTAGCTTCTTTTTTATATGTTAACGATATACCCCTACTCTTTTCTCCAAT
AAATCTTGCAGGCAATCTTC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTLQEQIMKALHVQPVIDPKVEIRKRVDFLKDYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVEEIRNEGGNATFIAVRL
PYKVQKDEDDAQLALQFIQADQSVAFDIASTVDAFSNQYENLLGESLTDFNKGNVKARIRMVTQYAIGGQKGLLVIGTDH
AAEAVTGFFTKFGDGGADLLPLTGLTKRQGRALLQELGADERLYLKMPTADLLDEKPGQADETELGITYDQLDDYLEGKA
VPADVAEKIEKRYTVSEHKRQVPASMFDDWWK

Sequences:

>Translated_272_residues
MTLQEQIMKALHVQPVIDPKVEIRKRVDFLKDYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVEEIRNEGGNATFIAVRL
PYKVQKDEDDAQLALQFIQADQSVAFDIASTVDAFSNQYENLLGESLTDFNKGNVKARIRMVTQYAIGGQKGLLVIGTDH
AAEAVTGFFTKFGDGGADLLPLTGLTKRQGRALLQELGADERLYLKMPTADLLDEKPGQADETELGITYDQLDDYLEGKA
VPADVAEKIEKRYTVSEHKRQVPASMFDDWWK
>Mature_271_residues
TLQEQIMKALHVQPVIDPKVEIRKRVDFLKDYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVEEIRNEGGNATFIAVRLP
YKVQKDEDDAQLALQFIQADQSVAFDIASTVDAFSNQYENLLGESLTDFNKGNVKARIRMVTQYAIGGQKGLLVIGTDHA
AEAVTGFFTKFGDGGADLLPLTGLTKRQGRALLQELGADERLYLKMPTADLLDEKPGQADETELGITYDQLDDYLEGKAV
PADVAEKIEKRYTVSEHKRQVPASMFDDWWK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=313, Percent_Identity=26.1980830670927, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788036, Length=275, Percent_Identity=56, Blast_Score=292, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17538756, Length=259, Percent_Identity=27.7992277992278, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30041; Mature: 29910

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQEQIMKALHVQPVIDPKVEIRKRVDFLKDYVKKTGAKGFVLGISGGQDSTLAGRLAQ
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
LAVEEIRNEGGNATFIAVRLPYKVQKDEDDAQLALQFIQADQSVAFDIASTVDAFSNQYE
HHHHHHHCCCCCEEEEEEECCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NLLGESLTDFNKGNVKARIRMVTQYAIGGQKGLLVIGTDHAAEAVTGFFTKFGDGGADLL
HHHHHHHHCCCCCCCEEHHEEHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEE
PLTGLTKRQGRALLQELGADERLYLKMPTADLLDEKPGQADETELGITYDQLDDYLEGKA
ECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCC
VPADVAEKIEKRYTVSEHKRQVPASMFDDWWK
CCHHHHHHHHHHCCHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
TLQEQIMKALHVQPVIDPKVEIRKRVDFLKDYVKKTGAKGFVLGISGGQDSTLAGRLAQ
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
LAVEEIRNEGGNATFIAVRLPYKVQKDEDDAQLALQFIQADQSVAFDIASTVDAFSNQYE
HHHHHHHCCCCCEEEEEEECCEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NLLGESLTDFNKGNVKARIRMVTQYAIGGQKGLLVIGTDHAAEAVTGFFTKFGDGGADLL
HHHHHHHHCCCCCCCEEHHEEHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEE
PLTGLTKRQGRALLQELGADERLYLKMPTADLLDEKPGQADETELGITYDQLDDYLEGKA
ECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCC
VPADVAEKIEKRYTVSEHKRQVPASMFDDWWK
CCHHHHHHHHHHCCHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA