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The map label for this gene is yrkH [H]

Identifier: 218233109

GI number: 218233109

Start: 3398564

End: 3400000

Strand: Reverse

Name: yrkH [H]

Synonym: BCB4264_A3523

Alternate gene names: 218233109

Gene position: 3400000-3398564 (Counterclockwise)

Preceding gene: 218233097

Following gene: 218234583

Centisome position: 62.74

GC content: 33.54

Gene sequence:

>1437_bases
ATGCTTTTAAAATATTTTTATGATGAAAAATTAGCACATGCTTCCTATTTAGTCGGTTGTCAGAAAGAAGGCGTAGCAAT
CGTTATTGATCCTAGTCGCTATATAGAACAATATATAGAATTTGCTAAGAAAGAGGGGATGGAAGTAATTGCTGCGGCTG
AGACTCATATTCATGCTGATTTTCTTTCTGGGTCTAGAGAACTTTCTAATCTTTACCATGCAAATTTATATGTATCTGAT
GAAGGAGATTGTGATTGGAAATATCAATATCTTAACGAAGGTCGATACAAATTGGTTAGAGAGGGCACAGAGTTTAAAGT
AGGTCATATAAAATTTAATGTAATTCACACCCCAGGGCATACGCCTGAAAGTATTTCTTTTTTAGTAACTGATACAAGTC
AAAATAATTATACGAATGATAAACCGATAGGAATATTCACAGGTGATTTTATATTTGTAGGAGATATAGGAAGACCGGAT
TTATTAGAAACTGCAGTCGGTATTAAAGATACTGCAAAAATAGGAGCGAAACAATTATTTGATTCTATACAAAAAATAAA
AATACTTCCTGATTATTTGCAAATATGGCCATCACATGGTGCGGGAAGCGCATGTGGAAAAGCATTAGGAGCAATTCCAA
CTTCTACATTAGGTTATGAAAAGATGTTCAATTGGGCATTTCGGTGTAATGAAGAAAGTGATTTTATATCGACTTTATTG
ACGGGACAGCCAGAGCCTCCAAAGTATTTTTCATTAATGAAGAATTTAAATAAGTATGGTCCGCCAATTCGTAAGAAGAG
AAAAAGTACTGCTATTAATACAGTAGAAGAACTTCAAGAAATTTTGAAGAGCGTTTATCAAATAGTTGATATAAGGGATG
TAGAGAGTTTTGCTGCAGGTCATATCGAGAAGTCAATTAACATACCTTATAACAATTCTTTCACAACTTGGTGTGGATGG
TTATTAGATTATAAAAAAGAAACTTTAATAATTCTAGATGAGGAAAAGGTTAAAGTAGAGGCAGTTATTAGAGAATTTGA
ATCTATTGGGTTAGATAATATTATTGCTTTTGCACCCTTAAAAGTAATACAAAGACTTGATAGATTTGAAAGTTACAAAG
AAAAAACATCGATTGAATTATATCCGCATATAAAAGGTGGAAGCGTTAAGGTTATCGATGTGCGTAGTAAAAAAGAGTGG
GAGGAAGGACACCTTCATGATGCAATACATATCACTTTAGGTAATCTATTTGAACAACTAGATTATATACCGAAAGATTT
TCCAATAGTTTTGCAATGTCGAACCGGATTACGTTCCGCTATAGCAGCTAGTATTTTACAAAGAGCTGGTATAAAGGAAG
TAGTGAATTTAAAGGGAGGATTTCTTGCATGGAAAGAAGAAGGGCTTCCTTATACAACATGCAATCTCAAGGTGTAG

Upstream 100 bases:

>100_bases
GTATTGGAATTTAATGTTGAAAGGGATTCTATAGGAGAAGATAAAAAGTGGTTACAAAAAATGAAAATTAATTTGAAATA
ACTAATTGGAGGGAATAGCT

Downstream 100 bases:

>100_bases
GAAAATATTATATGCTAGGTAAGAATAATTAAAAGAAGTTATAGATGACGTGTTAGAAAAGGGAATCATAATTGTCGCAG
ATTCAGGAAATACATTAGGA

Product: hydroxyacylglutathione hydrolase

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPSRYIEQYIEFAKKEGMEVIAAAETHIHADFLSGSRELSNLYHANLYVSD
EGDCDWKYQYLNEGRYKLVREGTEFKVGHIKFNVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPD
LLETAVGIKDTAKIGAKQLFDSIQKIKILPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFRCNEESDFISTLL
TGQPEPPKYFSLMKNLNKYGPPIRKKRKSTAINTVEELQEILKSVYQIVDIRDVESFAAGHIEKSINIPYNNSFTTWCGW
LLDYKKETLIILDEEKVKVEAVIREFESIGLDNIIAFAPLKVIQRLDRFESYKEKTSIELYPHIKGGSVKVIDVRSKKEW
EEGHLHDAIHITLGNLFEQLDYIPKDFPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWKEEGLPYTTCNLKV

Sequences:

>Translated_478_residues
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPSRYIEQYIEFAKKEGMEVIAAAETHIHADFLSGSRELSNLYHANLYVSD
EGDCDWKYQYLNEGRYKLVREGTEFKVGHIKFNVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPD
LLETAVGIKDTAKIGAKQLFDSIQKIKILPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFRCNEESDFISTLL
TGQPEPPKYFSLMKNLNKYGPPIRKKRKSTAINTVEELQEILKSVYQIVDIRDVESFAAGHIEKSINIPYNNSFTTWCGW
LLDYKKETLIILDEEKVKVEAVIREFESIGLDNIIAFAPLKVIQRLDRFESYKEKTSIELYPHIKGGSVKVIDVRSKKEW
EEGHLHDAIHITLGNLFEQLDYIPKDFPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWKEEGLPYTTCNLKV
>Mature_478_residues
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPSRYIEQYIEFAKKEGMEVIAAAETHIHADFLSGSRELSNLYHANLYVSD
EGDCDWKYQYLNEGRYKLVREGTEFKVGHIKFNVIHTPGHTPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPD
LLETAVGIKDTAKIGAKQLFDSIQKIKILPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFRCNEESDFISTLL
TGQPEPPKYFSLMKNLNKYGPPIRKKRKSTAINTVEELQEILKSVYQIVDIRDVESFAAGHIEKSINIPYNNSFTTWCGW
LLDYKKETLIILDEEKVKVEAVIREFESIGLDNIIAFAPLKVIQRLDRFESYKEKTSIELYPHIKGGSVKVIDVRSKKEW
EEGHLHDAIHITLGNLFEQLDYIPKDFPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWKEEGLPYTTCNLKV

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787158, Length=222, Percent_Identity=25.6756756756757, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese [H]

EC number: 3.1.2.6

Molecular weight: Translated: 54168; Mature: 54168

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPSRYIEQYIEFAKKEGMEVIAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
FLSGSRELSNLYHANLYVSDEGDCDWKYQYLNEGRYKLVREGTEFKVGHIKFNVIHTPGH
HHCCHHHHHHHEEEEEEEECCCCCCEEEHHHCCCCEEEEECCCCEEEEEEEEEEEECCCC
TPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPDLLETAVGIKDTAKIGAKQLF
CCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHH
DSIQKIKILPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFRCNEESDFISTLL
HHHHHHHCCCHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHHHHHHH
TGQPEPPKYFSLMKNLNKYGPPIRKKRKSTAINTVEELQEILKSVYQIVDIRDVESFAAG
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIEKSINIPYNNSFTTWCGWLLDYKKETLIILDEEKVKVEAVIREFESIGLDNIIAFAPL
CCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHH
KVIQRLDRFESYKEKTSIELYPHIKGGSVKVIDVRSKKEWEEGHLHDAIHITLGNLFEQL
HHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHH
DYIPKDFPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWKEEGLPYTTCNLKV
HCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEECCCCCEEEEEEEC
>Mature Secondary Structure
MLLKYFYDEKLAHASYLVGCQKEGVAIVIDPSRYIEQYIEFAKKEGMEVIAAAETHIHAD
CEEHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
FLSGSRELSNLYHANLYVSDEGDCDWKYQYLNEGRYKLVREGTEFKVGHIKFNVIHTPGH
HHCCHHHHHHHEEEEEEEECCCCCCEEEHHHCCCCEEEEECCCCEEEEEEEEEEEECCCC
TPESISFLVTDTSQNNYTNDKPIGIFTGDFIFVGDIGRPDLLETAVGIKDTAKIGAKQLF
CCCCEEEEEEECCCCCCCCCCCEEEEECCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHH
DSIQKIKILPDYLQIWPSHGAGSACGKALGAIPTSTLGYEKMFNWAFRCNEESDFISTLL
HHHHHHHCCCHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHHHHHHH
TGQPEPPKYFSLMKNLNKYGPPIRKKRKSTAINTVEELQEILKSVYQIVDIRDVESFAAG
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIEKSINIPYNNSFTTWCGWLLDYKKETLIILDEEKVKVEAVIREFESIGLDNIIAFAPL
CCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHH
KVIQRLDRFESYKEKTSIELYPHIKGGSVKVIDVRSKKEWEEGHLHDAIHITLGNLFEQL
HHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHH
DYIPKDFPIVLQCRTGLRSAIAASILQRAGIKEVVNLKGGFLAWKEEGLPYTTCNLKV
HCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]