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The map label for this gene is priA [H]

Identifier: 218233101

GI number: 218233101

Start: 3888689

End: 3891094

Strand: Reverse

Name: priA [H]

Synonym: BCB4264_A3967

Alternate gene names: 218233101

Gene position: 3891094-3888689 (Counterclockwise)

Preceding gene: 218231578

Following gene: 218232337

Centisome position: 71.8

GC content: 35.87

Gene sequence:

>2406_bases
ATGAAATTTGCAAGTGTAATTGTTGATGTACCTGCCCGTCAGACAGATCGACCATTTGATTATATTATTCCTAAAAAATG
GGAAGATATTGTACAGACAGGAATGCGTGTAGTAGTTCCGTTTGGGCCAAGAAAATTGCAAGGTTTTATTATTGGGATTA
AAGATTCGGTGGATGTAGAAAGTAAGAAGTTAAAAACAATCCATGAAATATTAGATGTAACACCGGTTTTAAATGATGAA
TTATTAAAGCTTGGATATTGGCTTACAAGTGAAACGTTATGCTACATGATTTCAGCTTTTCAAGTGATGCTTCCAACTGC
GATAAAAGCAACGTATAAAAAACGTCTACAACTTTGTAAACAGGAAGAAATAGCACCTGAAATACTATATTTATTTCAGG
ATAAAGAAGCAATTGATTTTGAAGTAATCGAGCAACAGCCACATTTATACCGAACTATTCAACAAGAAATTAAAAATGGT
ACGGTTGAAGTCGTTTATCAAGTGAAAGATAAAGTACAAAAGAAGAAGCAAAGAGTGGTTCAGCCGGAGTTGCCAGAAGA
TAAATTGGAATTAGCGGCATTTGAACTGAAAAGTAAAAAACAACAAGATGTACTTTATTATTTTGTGGAAAATTATAAAA
GTACACCGTTGAAAGTAATTACAGAAGAGTTACAAATAACAGATGCTCCAATAAAAGCACTTGTTAAAAAGGGATTCATT
TCAGAAAAGTATGTGGAAGTATACCGAAATCCGTATGACGATGATGATTTTGAACAAACAAAACCATTTCCACTTACGGA
GGAACAAAAACAAGTTATTACACCGATTTTATCATCAATTACAAATGAAACTTATAATCCATTTTTACTATATGGTGTTA
CAGGAAGTGGAAAGACAGAAGTATATTTACAATCTATAGCAGCAGTGCTCAAGAAAGGAAAAGAAGCTATCGTTCTTGTT
CCTGAAATTGCACTAACGCCCCAAATGGTGGATCGTTTTAAAGGTAGGTTTGGCTCGCAAGTTGCAGTTCTTCATAGTGC
GTTGTCTGTTGGAGAAAAGTATGACGAGTGGCGTAAGATTTTAAGAAAAGAAGTGAAAGTTGTAGTTGGTGCGCGTTCAG
CTGTTTTTGCGCCTTTTGAGAATTTAGGGATTATTATTATTGATGAAGAGCATGAATCAAGTTACAAACAAGAAGATAAT
CCGAGGTATCATGCAAGAGATGTAGCTGTCTGGAGAGGACAATATCATAAATGTCCTATCGTTCTTGGTAGTGCGACACC
AACACTTGAATCGTTTGCAAGGGCGAAAAAAGGTGTTTATGAACTATTAACAATGGAAAAACGTATGAATGAACAAGCTT
TACCAACGGTAGAGATTGTTGACATGCGAGAAGAACTTCGTGACGGGAATCGATCAATGTTTTCTAAGGCGCTGCATGAA
AAAATAGCAGATCGATTAGAAAAGAAAGAACAAATGGTACTCTTTTTAAATAGAAGAGGTCATTCTACATTTGTTATGTG
CCGTGATTGTGGGTATGTTGTCCAATGTCCGCATTGTGATATTTCATTGACATATCATAAAATGAATCATCGTTTAAAAT
GTCATTATTGTAGTTACGAAGAGAATATGCCAACTGAGTGTCCAGCTTGTCAAAGTACATACATTCGTTTCTTTGGTACA
GGTACACAAAAGGTAGAAGAAGAAATTACAAAACTATTTCCAGAGGCACGAGTCATTCGAATGGATGTAGATACGACAAG
TCGAAAAGGAATGCATGAAAAATTATTAAAGGCGTTTGGGGAAGAAAAAGCAGATATATTACTTGGAACACAAATGATTG
CTAAAGGATTAGATTTTCCGAAAGTAACGCTCGTTGGGGTTTTAACTGCAGATACGATGCTTCACTTACCTGATTTTCGG
GCGAGTGAAAAGACATATCAGTTACTGACGCAAGTAAGTGGACGAGCGGGTAGACATGAATTGCCAGGGGAAGTTGTGAT
TCAAACGTATACGCCAGAACATTACAGTATAGAGTTAGCGAAGAACCAACAATATGATGTGTTTTTTGACCAAGAAATGC
AAATGAGACGAACAAGACAATATCCCCCATATTACTATGTTGTACTCGTAACTGTATCTCATCCAGAATTATTAAAAGCG
GTACAAGTGACGGAAAAAATTGCCTCGTATTTACGAACGCATTGCTCACAGCAAACAATGGTGCTAGGACCAGTTGCTTC
AGCGATTCCAAGGATAAAAGATAGATATCGTTATCAATGCATGATAAAATACAAACGGGAACCAAACTTAAAGAACGTGC
TCAAAATGGTAAATGAACATTATCAAGCAGAAATGCAAAAAGAGCTACAAATCTCAATTGATTTTAATCCAACAATGTTA
ATGTAG

Upstream 100 bases:

>100_bases
GAAAAGATGGAAAAGTTATTGAGTTGCCACTTTTAATGAAAAAAGAGGTAGCTCGTGAAATATTGAAGCAAATTGAAATG
ATGTTAGAAGATGATCGTTT

Downstream 100 bases:

>100_bases
CGGAGGGAAATATGGCAGTTTTAGAAATAATAAAGCATCCAAATGAAGTGTTAGAAACACCATGCGAAAGAGTAATTAAC
TTTGATAAAAAGTTAGTGAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKDSVDVESKKLKTIHEILDVTPVLNDE
LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLCKQEEIAPEILYLFQDKEAIDFEVIEQQPHLYRTIQQEIKNG
TVEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSTPLKVITEELQITDAPIKALVKKGFI
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLKKGKEAIVLV
PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN
PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTECPACQSTYIRFFGT
GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR
ASEKTYQLLTQVSGRAGRHELPGEVVIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
VQVTEKIASYLRTHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML
M

Sequences:

>Translated_801_residues
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKDSVDVESKKLKTIHEILDVTPVLNDE
LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLCKQEEIAPEILYLFQDKEAIDFEVIEQQPHLYRTIQQEIKNG
TVEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSTPLKVITEELQITDAPIKALVKKGFI
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLKKGKEAIVLV
PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN
PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTECPACQSTYIRFFGT
GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR
ASEKTYQLLTQVSGRAGRHELPGEVVIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
VQVTEKIASYLRTHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML
M
>Mature_801_residues
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKDSVDVESKKLKTIHEILDVTPVLNDE
LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLCKQEEIAPEILYLFQDKEAIDFEVIEQQPHLYRTIQQEIKNG
TVEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSTPLKVITEELQITDAPIKALVKKGFI
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLKKGKEAIVLV
PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN
PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTECPACQSTYIRFFGT
GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR
ASEKTYQLLTQVSGRAGRHELPGEVVIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
VQVTEKIASYLRTHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML
M

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=556, Percent_Identity=39.3884892086331, Blast_Score=424, Evalue=1e-119,
Organism=Escherichia coli, GI1787357, Length=191, Percent_Identity=25.130890052356, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 92662; Mature: 92662

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKDSVDVE
CCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCCH
SKKLKTIHEILDVTPVLNDELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLCK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEIAPEILYLFQDKEAIDFEVIEQQPHLYRTIQQEIKNGTVEVVYQVKDKVQKKKQRVV
HHCCCHHHEEEECCCCCCCHHHHHCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCC
QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSTPLKVITEELQITDAPIKALVKKGFI
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
VYLQSIAAVLKKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI
HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI
HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE
VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE
HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC
ENMPTECPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG
CCCCCCCCCHHHHHEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE
CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC
LPGEVVIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH
VQVTEKIASYLRTHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH
HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH
YQAEMQKELQISIDFNPTMLM
HHHHHCCCEEEEEECCCCCCC
>Mature Secondary Structure
MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKDSVDVE
CCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCCH
SKKLKTIHEILDVTPVLNDELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLCK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEEIAPEILYLFQDKEAIDFEVIEQQPHLYRTIQQEIKNGTVEVVYQVKDKVQKKKQRVV
HHCCCHHHEEEECCCCCCCHHHHHCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCC
QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSTPLKVITEELQITDAPIKALVKKGFI
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC
SEKYVEVYRNPYDDDDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
VYLQSIAAVLKKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI
HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI
HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE
VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE
HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC
ENMPTECPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG
CCCCCCCCCHHHHHEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC
EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE
CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC
LPGEVVIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA
CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH
VQVTEKIASYLRTHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH
HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH
YQAEMQKELQISIDFNPTMLM
HHHHHCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]