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The map label for this gene is nirA [H]

Identifier: 218232883

GI number: 218232883

Start: 1416740

End: 1418362

Strand: Direct

Name: nirA [H]

Synonym: BCB4264_A1479

Alternate gene names: 218232883

Gene position: 1416740-1418362 (Clockwise)

Preceding gene: 218234957

Following gene: 218230793

Centisome position: 26.14

GC content: 36.35

Gene sequence:

>1623_bases
ATGAGTTATGAAAAAGTATGGGCTAACAATGAAAAATTAAATCAGACCGAGAAAAATAAATTAGAAAAAGATGGCTTAGA
AATTTTTAATGATATTCCTTACTATGCGGAAAATGGTTTCGAATCTATTCCGAAAGAAGAGTGGGATGCCTTTAAATGGG
CAGGCTTGTATTTACAAAGACCGAAAGAAGCTGGTTATTTTATGATGCGGGTAAATATTCCGTCAGGGATTATTACAAAT
GCACAGGCAGAAGTACTTGCTTCTATCGCTGAAGATTATGGACGTAATGTAATAGATATTACGACGAGACAGGCGATTCA
GTTTCACTGGTTAGAAATTCAGCAAATTCCAGATATTTTTAAAAGATTAGCAAGAGTTGGGTTATCTTCAGCGGGGGCTT
GTGGTGATATAACGCGGAATATTACAGGTAATCCGCTTGCTGGTATTGATGCAAATGAACTATTCGATACAACGCATATT
GTAAAAGAAGTATATGATTACTTCCAACATAATGAAGAGTTTTCTAACTTGCCACGTAAATTTAAAATGTCTATTAGTTC
TAATATTTATAATTCAGCAAACGCAGAAATTAACTGTGTTGCATTTACGCCTGCAACGAAAGAAATAGATGGTGAGAAAA
AAGTTGGTTTTCATATAAAAGTAGGTGGAGGATTATCAGCTCGACCGTATTTAGCTGATGAATTAGATGTATTCGTTTTA
CCAGAAGAAGTGAAGGCGGTGGCGATTGCGATTGCTACTATATTCCGTGATTTTGGATATCGTGAAAAACGTCATTTAGC
TCGGTTGAAATTTCTTGTTGCGGACTGGGGAGCAGAGAAATTTAAAGAGAAACTAATAGAGTATACAGGGCCTTTACAAA
GTAAAGGGGAAAGTGCACTCAAAGGATGGAATGCAGGCTATTTTTATGGTGTTCAAGACCAAAAACAAAAGGGATTAAAA
TATGTAGGTTTTAATGTACCGGTAGGTCGTTTACATGCAGAAGAAATGTTTGAGATTGCAAGAATCGCAAAGCAATATGG
AAATGGACAAATTCGTACATGTAACTCGCAAAACTTCATTATTCCGAACGTTCCTCCAGAAAATGTAAAAGGATTACTTA
GTGAACCGCTGTTTGAAGCGATATCTGCTAGCCCGAAATCATTTATTGGTCATGCGGTTTCATGTACCGGTATTGAATAT
TGTAATCTTGCACTAGTAGAAACGAAAGAAAGATTACGAAAAATTGCAGAATACTTAGATACACAAATTGCACTCGATGT
TCCAGTTCGAATTCATATGGTAGGCTGCCCGAATTCATGTGGTCAACGTCAAATTGCTGACATTGGGTTACAAGGGGTGA
AAATGAAGACGAAGGAAAAAGGTATTGTTGAAGCGTTTGAAATATACGTAGGTGGAACATTGTTAGATGGCGGTGCCTAC
AATCAAAAGTTAAAAGGAAAAATAGATGGCGAGGATCTACCTGATGTACTCGCATCATTTATAAGTTATTTCAAAGAGAA
TAAATTACCAGCCGAAACATTTTATGATTTTGTAGGTCGTATTGGTGTAGATACATTACAAATAGCGTTAAATAACGTGC
TAGAAGAAGTAATAGCGTCTTAG

Upstream 100 bases:

>100_bases
CGGAATTAATTGTAGAAACGCACAAATATTCTATTGAAGAATGTGCAGAACAAATCGTGAAGTACTTACAAGAGCGTAGT
TTTATATAGGGGGATAAACA

Downstream 100 bases:

>100_bases
GAGGGACGATATGGATTTATATCGATTTGAAGCTGTGTTAGTAAATAGTATTGTTCCGATTGTTGTTGTTGCTCAAAGTG
AAGAACAGGCATTTAAGCTT

Product: putative sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSYEKVWANNEKLNQTEKNKLEKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQRPKEAGYFMMRVNIPSGIITN
AQAEVLASIAEDYGRNVIDITTRQAIQFHWLEIQQIPDIFKRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHI
VKEVYDYFQHNEEFSNLPRKFKMSISSNIYNSANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESALKGWNAGYFYGVQDQKQKGLK
YVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFIIPNVPPENVKGLLSEPLFEAISASPKSFIGHAVSCTGIEY
CNLALVETKERLRKIAEYLDTQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRIGVDTLQIALNNVLEEVIAS

Sequences:

>Translated_540_residues
MSYEKVWANNEKLNQTEKNKLEKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQRPKEAGYFMMRVNIPSGIITN
AQAEVLASIAEDYGRNVIDITTRQAIQFHWLEIQQIPDIFKRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHI
VKEVYDYFQHNEEFSNLPRKFKMSISSNIYNSANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESALKGWNAGYFYGVQDQKQKGLK
YVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFIIPNVPPENVKGLLSEPLFEAISASPKSFIGHAVSCTGIEY
CNLALVETKERLRKIAEYLDTQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRIGVDTLQIALNNVLEEVIAS
>Mature_539_residues
SYEKVWANNEKLNQTEKNKLEKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQRPKEAGYFMMRVNIPSGIITNA
QAEVLASIAEDYGRNVIDITTRQAIQFHWLEIQQIPDIFKRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHIV
KEVYDYFQHNEEFSNLPRKFKMSISSNIYNSANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVLP
EEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESALKGWNAGYFYGVQDQKQKGLKY
VGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFIIPNVPPENVKGLLSEPLFEAISASPKSFIGHAVSCTGIEYC
NLALVETKERLRKIAEYLDTQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAYN
QKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRIGVDTLQIALNNVLEEVIAS

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=510, Percent_Identity=25.4901960784314, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322597, Length=379, Percent_Identity=26.1213720316623, Blast_Score=103, Evalue=7e-23,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 60424; Mature: 60292

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEKVWANNEKLNQTEKNKLEKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQR
CCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHCHHHHHHHHEEC
PKEAGYFMMRVNIPSGIITNAQAEVLASIAEDYGRNVIDITTRQAIQFHWLEIQQIPDIF
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHH
KRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHIVKEVYDYFQHNEEFSNLPRK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHH
FKMSISSNIYNSANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
EEHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESAL
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHH
KGWNAGYFYGVQDQKQKGLKYVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFI
CCCCCCEEECCCHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
IPNVPPENVKGLLSEPLFEAISASPKSFIGHAVSCTGIEYCNLALVETKERLRKIAEYLD
ECCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEECCHHHHCCHHHHHHHHHHHHHHHHC
TQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
CCEEEECCEEEEEEECCCCCCCHHHHHHCCCCCEECCHHCCHHHHHHHEECCEEECCCCC
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRIGVDTLQIALNNVLEEVIAS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYEKVWANNEKLNQTEKNKLEKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQR
CCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHCHHHHHHHHEEC
PKEAGYFMMRVNIPSGIITNAQAEVLASIAEDYGRNVIDITTRQAIQFHWLEIQQIPDIF
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHH
KRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHIVKEVYDYFQHNEEFSNLPRK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHH
FKMSISSNIYNSANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
EEHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKLIEYTGPLQSKGESAL
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHH
KGWNAGYFYGVQDQKQKGLKYVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFI
CCCCCCEEECCCHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
IPNVPPENVKGLLSEPLFEAISASPKSFIGHAVSCTGIEYCNLALVETKERLRKIAEYLD
ECCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEECCHHHHCCHHHHHHHHHHHHHHHHC
TQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
CCEEEECCEEEEEEECCCCCCCHHHHHHCCCCCEECCHHCCHHHHHHHEECCEEECCCCC
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRIGVDTLQIALNNVLEEVIAS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]