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The map label for this gene is hdhA [C]

Identifier: 218232829

GI number: 218232829

Start: 4036839

End: 4037348

Strand: Reverse

Name: hdhA [C]

Synonym: BCB4264_A4123

Alternate gene names: 218232829

Gene position: 4037348-4036839 (Counterclockwise)

Preceding gene: 218234146

Following gene: 218235421

Centisome position: 74.5

GC content: 37.25

Gene sequence:

>510_bases
ATGACGGTAAGACTTATAGAAGGGGTAATGAAAATGAAGCTTGCAGGAAAAGTTGCTATCGTAACTGGCGGTGGCATCGG
TATTGGACGTAATACAGCTCTTTTGTTGGCTAAACAAGGCGCAAAAGTTATTGTGACGGACATTGATCAAGAGAGTGGAC
AAGCAACAGTGGAGGAAATTATGAATCTAGGCGGAGAAGCACTTTTTGTATCCTATGATATGGGAAAGCAAGGAGATTGG
CAACGCGTTATTAGCATTACTTTGAATGCATTCAGTCGCATTGATATGCTTTTTCAAAATGCTGGTTTATATAAAATAGA
TTCTATTTTTTCTCGCCAACAAGAGAACGATTCCAATGTACTTTGTATTAATGACGTTTGGATAGAGATAAAACAATTAA
CGTCATCTTTTATAAAACAGCAAGAAGAAGTGGTGCTTAGTGATTTACCGATTTTCGGTATTATTAGCACGAAAGGACAA
TCATTTCATACAGTAGAAGCCCTAGTATAA

Upstream 100 bases:

>100_bases
AGCGTAATAATAAGAAAAAAGAAGGATAAAAAGTAAACGTTTACAAATTAAATAGCAATTTTTCTTGCTTGCAGATTAAT
CTTATAAAGGAGTATACTGA

Downstream 100 bases:

>100_bases
TAATGAAAACCGCCCTCAATATATGTGGACGGTTTTTTGTATGTGTATAGTAAAGATAAATTTTTTAGCGAATACGTTGT
TCAGTTTGGTTATCAAAGAA

Product: hypothetical protein

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MTVRLIEGVMKMKLAGKVAIVTGGGIGIGRNTALLLAKQGAKVIVTDIDQESGQATVEEIMNLGGEALFVSYDMGKQGDW
QRVISITLNAFSRIDMLFQNAGLYKIDSIFSRQQENDSNVLCINDVWIEIKQLTSSFIKQQEEVVLSDLPIFGIISTKGQ
SFHTVEALV

Sequences:

>Translated_169_residues
MTVRLIEGVMKMKLAGKVAIVTGGGIGIGRNTALLLAKQGAKVIVTDIDQESGQATVEEIMNLGGEALFVSYDMGKQGDW
QRVISITLNAFSRIDMLFQNAGLYKIDSIFSRQQENDSNVLCINDVWIEIKQLTSSFIKQQEEVVLSDLPIFGIISTKGQ
SFHTVEALV
>Mature_168_residues
TVRLIEGVMKMKLAGKVAIVTGGGIGIGRNTALLLAKQGAKVIVTDIDQESGQATVEEIMNLGGEALFVSYDMGKQGDWQ
RVISITLNAFSRIDMLFQNAGLYKIDSIFSRQQENDSNVLCINDVWIEIKQLTSSFIKQQEEVVLSDLPIFGIISTKGQS
FHTVEALV

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4504505, Length=120, Percent_Identity=34.1666666666667, Blast_Score=68, Evalue=4e-12,
Organism=Escherichia coli, GI1787905, Length=92, Percent_Identity=34.7826086956522, Blast_Score=68, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=102, Percent_Identity=41.1764705882353, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=117, Percent_Identity=37.6068376068376, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=93, Percent_Identity=43.010752688172, Blast_Score=66, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=96, Percent_Identity=39.5833333333333, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17551412, Length=96, Percent_Identity=41.6666666666667, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=93, Percent_Identity=37.6344086021505, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=121, Percent_Identity=32.2314049586777, Blast_Score=63, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=93, Percent_Identity=37.6344086021505, Blast_Score=62, Evalue=1e-10,
Organism=Drosophila melanogaster, GI18859837, Length=144, Percent_Identity=35.4166666666667, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 18511; Mature: 18380

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRLIEGVMKMKLAGKVAIVTGGGIGIGRNTALLLAKQGAKVIVTDIDQESGQATVEEI
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHH
MNLGGEALFVSYDMGKQGDWQRVISITLNAFSRIDMLFQNAGLYKIDSIFSRQQENDSNV
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCE
LCINDVWIEIKQLTSSFIKQQEEVVLSDLPIFGIISTKGQSFHTVEALV
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECC
>Mature Secondary Structure 
TVRLIEGVMKMKLAGKVAIVTGGGIGIGRNTALLLAKQGAKVIVTDIDQESGQATVEEI
CHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHH
MNLGGEALFVSYDMGKQGDWQRVISITLNAFSRIDMLFQNAGLYKIDSIFSRQQENDSNV
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCE
LCINDVWIEIKQLTSSFIKQQEEVVLSDLPIFGIISTKGQSFHTVEALV
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]