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The map label for this gene is rfbB [H]

Identifier: 218232660

GI number: 218232660

Start: 1235181

End: 1236152

Strand: Direct

Name: rfbB [H]

Synonym: BCB4264_A1269

Alternate gene names: 218232660

Gene position: 1235181-1236152 (Clockwise)

Preceding gene: 218233453

Following gene: 218232620

Centisome position: 22.79

GC content: 34.47

Gene sequence:

>972_bases
ATGAATATATTAGTAACAGGTGGGGCTGGATTTATTGGAAGTAATTTCGTTCATTACATGCTACAAAGTTATGAAACATA
TAAAATTATTAATTACGATGCATTAACATATAGCGGGAATTTAAATAACGTAAAATCTCTTCAGGATCACCCGAATTACT
TTTTTGTAAAGGGAGAAATTCAAAATGGAGAGCTATTAGAACACGTTATAAAAGAGCGGGACGTACAAGTGATTGTAAAT
TTTGCAGCTGAATCACATGTGGACCGTAGTATTGAAAATCCGATTCCTTTTTATGATACAAATGTAATTGGGACGGTCAC
TTTATTAGAACTAGTTAAAAAATATTCGCATATAAAACTTGTGCAAGTATCAACGGATGAAGTGTATGGCTCTTTAGGAA
AAACAGGGAAGTTCACAGAGAAAACGCCGTTAGCTCCAAATAGTCCATATTCTTCAAGTAAGGCGAGCGCGGACATGATA
GCTCTATCTTACTATAAAACATATCAATTACCGGTTATAGTAACGCGTTGTTCTAACAATTATGGACCGTACCAGTATCC
TGAAAAATTAATTCCATTAATGGTTACGAATGCACTAGAAGGAAAAAAACTACCGTTATATGGTGATGGTTTAAATGTAA
GAGATTGGATACATGTAACGGATCATTGCAGTGCGATTGACGTTGTTTTGCATAAGGGACGTGTCGGAGAAGTATATAAT
ATAGGTGGAAATAATGAAAAAACAAATGTAGAAGTTGTGGAACAAATTATTACTCTTTTAGGAAAAACAAAAAAAGACAT
TGAATATGTTACAGATCGTTTAGGGCACGATCGTCGTTATGCGATTGATGCAGAGAAAATGAAGAATGAATTTGATTGGG
AACCGAAATATACGTTTGAACAAGGATTACAAGAGACGGTGAAATGGTATGAAAAGAATAAGGAATGGTGGAAACCACTA
AAAGACCAGTAA

Upstream 100 bases:

>100_bases
AATTCCTTGGCCAGTAACCGATCCTATATTGTCCGATAAGGATCAGATATTACCATTATTAGAGGAATATGAAGATAGTT
TTTAAGTAGGAGCGTTGTTT

Downstream 100 bases:

>100_bases
GGGGCATGTTAAATGAAAGAGAGGGTTATCATAACAGGAGCAAACGGTCAATTAGGAAAGCAACTATTTGAAGAGTTAGA
CTCTGAAGAATATGATATAT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINYDALTYSGNLNNVKSLQDHPNYFFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYSHIKLVQVSTDEVYGSLGKTGKFTEKTPLAPNSPYSSSKASADMI
ALSYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWIHVTDHCSAIDVVLHKGRVGEVYN
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFEQGLQETVKWYEKNKEWWKPL
KDQ

Sequences:

>Translated_323_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINYDALTYSGNLNNVKSLQDHPNYFFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYSHIKLVQVSTDEVYGSLGKTGKFTEKTPLAPNSPYSSSKASADMI
ALSYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWIHVTDHCSAIDVVLHKGRVGEVYN
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFEQGLQETVKWYEKNKEWWKPL
KDQ
>Mature_323_residues
MNILVTGGAGFIGSNFVHYMLQSYETYKIINYDALTYSGNLNNVKSLQDHPNYFFVKGEIQNGELLEHVIKERDVQVIVN
FAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYSHIKLVQVSTDEVYGSLGKTGKFTEKTPLAPNSPYSSSKASADMI
ALSYYKTYQLPVIVTRCSNNYGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWIHVTDHCSAIDVVLHKGRVGEVYN
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFEQGLQETVKWYEKNKEWWKPL
KDQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=323, Percent_Identity=38.0804953560372, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI42516563, Length=321, Percent_Identity=27.7258566978193, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI4504031, Length=334, Percent_Identity=25.4491017964072, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI56237023, Length=342, Percent_Identity=25.1461988304094, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI56118217, Length=342, Percent_Identity=25.1461988304094, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI189083684, Length=342, Percent_Identity=25.1461988304094, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI48994969, Length=341, Percent_Identity=46.9208211143695, Blast_Score=321, Evalue=4e-89,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=45.4545454545455, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1786974, Length=339, Percent_Identity=27.1386430678466, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788366, Length=231, Percent_Identity=26.8398268398268, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=39.1304347826087, Blast_Score=227, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI115532424, Length=318, Percent_Identity=32.7044025157233, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=25.3776435045317, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=27.2727272727273, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=27.1186440677966, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI133901786, Length=342, Percent_Identity=23.9766081871345, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=342, Percent_Identity=23.9766081871345, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=342, Percent_Identity=23.9766081871345, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=342, Percent_Identity=23.9766081871345, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=342, Percent_Identity=23.9766081871345, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=346, Percent_Identity=28.0346820809249, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356223, Length=321, Percent_Identity=26.791277258567, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI19923002, Length=343, Percent_Identity=27.6967930029155, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24158427, Length=340, Percent_Identity=23.2352941176471, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36953; Mature: 36953

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGGAGFIGSNFVHYMLQSYETYKIINYDALTYSGNLNNVKSLQDHPNYFFVKGEI
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHHHHCCCCEEEEEEEC
QNGELLEHVIKERDVQVIVNFAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYSHIKL
CCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEE
VQVSTDEVYGSLGKTGKFTEKTPLAPNSPYSSSKASADMIALSYYKTYQLPVIVTRCSNN
EEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEHHHEECCEEEEEEECCC
YGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWIHVTDHCSAIDVVLHKGRVGEVYN
CCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCHHEEEECCCCCHHHHHHHCCCCCCEEE
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFE
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCHHHH
QGLQETVKWYEKNKEWWKPLKDQ
HHHHHHHHHHHCCHHHCCCCCCC
>Mature Secondary Structure
MNILVTGGAGFIGSNFVHYMLQSYETYKIINYDALTYSGNLNNVKSLQDHPNYFFVKGEI
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHHHHCCCCEEEEEEEC
QNGELLEHVIKERDVQVIVNFAAESHVDRSIENPIPFYDTNVIGTVTLLELVKKYSHIKL
CCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEE
VQVSTDEVYGSLGKTGKFTEKTPLAPNSPYSSSKASADMIALSYYKTYQLPVIVTRCSNN
EEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEHHHEECCEEEEEEECCC
YGPYQYPEKLIPLMVTNALEGKKLPLYGDGLNVRDWIHVTDHCSAIDVVLHKGRVGEVYN
CCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCHHEEEECCCCCHHHHHHHCCCCCCEEE
IGGNNEKTNVEVVEQIITLLGKTKKDIEYVTDRLGHDRRYAIDAEKMKNEFDWEPKYTFE
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCCHHHH
QGLQETVKWYEKNKEWWKPLKDQ
HHHHHHHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]