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The map label for this gene is ilvD [H]

Identifier: 218232633

GI number: 218232633

Start: 1759527

End: 1761200

Strand: Direct

Name: ilvD [H]

Synonym: BCB4264_A1854

Alternate gene names: 218232633

Gene position: 1759527-1761200 (Clockwise)

Preceding gene: 218232349

Following gene: 218232474

Centisome position: 32.47

GC content: 40.5

Gene sequence:

>1674_bases
ATGAGAAGTGACATGATTAAAAAAGGTTTTGATAAAGCGCCGCACCGTAGTTTATTAAAAGCAACTGGTTTGAAAGATGA
AGATTTTGATAAACCTTTTATAGCGATTTGTAATTCTTTTATTGAAATTATTCCAGGGCATAAACACTTGAACGAGTTTG
GAAAACTTGTAAAAGAGGCAGTTCGTGCGGCAGGTATGGTACCATTCGAATTCAATACAATTGGAGTTGACGATGGTATT
GCGATGGGGCATATTGGTATGCGTTATTCTCTTCCGAGTCGTGAAATAATTGCAGATTCAGTAGAAACGGTTGTAAATGC
ACACTGGTTTGACGGCATGATTTGTATCCCAAACTGTGACAAAATTACACCAGGTATGATGATGGCTGCACTACGTATTA
ATATTCCGACTGTGTTTGTTTCAGGCGGCCCGATGGCAGCAGGAAAAACATCTAAAGGAGAAGTTGTTGATCTGAGTTCC
GTTTTTGAAGGAGTAGGAGCTTATCAATCTGGGAAAATTTCAGAAGAAGAATTAAAAGATATTGAAGATCATGGCTGTCC
ATCTTGTGGATCTTGTTCGGGTATGTTTACCGCGAACTCTATGAATTGTTTATGTGAAGTGTTAGGTTTAGCTCTTCCTG
GTAACGGGAGTATTTTAGCAATTGATCCAAGACGTGAAGAGTTAATTAAACAAGCAGCAGAGAAATTGAAAATTTTAATT
GAAAGAGATATTAAACCAAGAGACATTGTAACGGAAGAAGCAATTGATGATGCGTTTGCGCTTGATATGGCGATGGGCGG
TTCAACAAATACAGTGTTGCATACATTGGCGCTCGCGCAAGAGGCTGGATTAGATTACGATATGAACCGTATTGATGCCG
TTTCAAGACGTGTACCACATTTATGTAAAGTAAGCCCTGCTTCCAATTGGCATATGGAAGATATTGATCGTGCGGGCGGG
ATTAGTGCAATTTTGAAAGAGATGAGCCGGAAAGAAGGAGTACTTCATCTTGATCGAATAACTGCTACGGGGCAAACATT
AAGAGAAAATATTGCTGAGGCAGAGATTAAAGATAAGGAAGTTATTCACTCTCTTGAAAACCCTCATAGTGAGGAAGGAG
GATTGCGTATATTAAAAGGCAACCTTGCGAAAGATGGAGCGGTTATTAAAAGCGGTGCAACAGAGGTAAAGCGTTTTGAA
GGACCTTGCGTTATTTTTAATTCACAAGATGAGGCACTTGCTGGCATTATGCTTGGGAAAGTGAAAAAAGGAGATGTAGT
TGTCATTCGTTATGAAGGACCAAGAGGTGGCCCTGGTATGCCAGAAATGTTAGCACCAACATCAGCAATTGCTGGAATGG
GATTAGGTGCTGAGGTTGCATTATTAACGGATGGACGTTTCTCTGGAGCTTCACGTGGAATTTCAGTAGGACATATTTCA
CCAGAAGCAGCAGCGGGCGGGATGATTGCACTTCTTGAACAAGGGGATATTGTATGTATTGATGTTGAAGAACGTTTGTT
AGAAGTAAGAGTGAGTGATGAGGAATTAGAAAAGCGTAAAAAAGAATGGAAACGACCAGAACCGAAAGTGAAAACTGGCT
GGCTTGGACGTTATGCACAAATGGTAACATCGGCGAATACAGGGGCTGTTCTGAAAATCCCAAATTTTGATTGA

Upstream 100 bases:

>100_bases
CTTCGTGAAATGATGAGTTGGATTCATGCACCGAAAGAATTAGTAAAGAAATAGAGTAAGAGAGTATAAATGAAAAATAT
GTAAGAGGGGATTTGACAAG

Downstream 100 bases:

>100_bases
GCCAATTTAAAAGAGATAAGGATGATATATAATGGTGCAAAAGGTAAAGGAGAGAGTGAAAATTGAAGATATCTTAATGG
CTCATAATTGCATGAAAGAT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MRSDMIKKGFDKAPHRSLLKATGLKDEDFDKPFIAICNSFIEIIPGHKHLNEFGKLVKEAVRAAGMVPFEFNTIGVDDGI
AMGHIGMRYSLPSREIIADSVETVVNAHWFDGMICIPNCDKITPGMMMAALRINIPTVFVSGGPMAAGKTSKGEVVDLSS
VFEGVGAYQSGKISEEELKDIEDHGCPSCGSCSGMFTANSMNCLCEVLGLALPGNGSILAIDPRREELIKQAAEKLKILI
ERDIKPRDIVTEEAIDDAFALDMAMGGSTNTVLHTLALAQEAGLDYDMNRIDAVSRRVPHLCKVSPASNWHMEDIDRAGG
ISAILKEMSRKEGVLHLDRITATGQTLRENIAEAEIKDKEVIHSLENPHSEEGGLRILKGNLAKDGAVIKSGATEVKRFE
GPCVIFNSQDEALAGIMLGKVKKGDVVVIRYEGPRGGPGMPEMLAPTSAIAGMGLGAEVALLTDGRFSGASRGISVGHIS
PEAAAGGMIALLEQGDIVCIDVEERLLEVRVSDEELEKRKKEWKRPEPKVKTGWLGRYAQMVTSANTGAVLKIPNFD

Sequences:

>Translated_557_residues
MRSDMIKKGFDKAPHRSLLKATGLKDEDFDKPFIAICNSFIEIIPGHKHLNEFGKLVKEAVRAAGMVPFEFNTIGVDDGI
AMGHIGMRYSLPSREIIADSVETVVNAHWFDGMICIPNCDKITPGMMMAALRINIPTVFVSGGPMAAGKTSKGEVVDLSS
VFEGVGAYQSGKISEEELKDIEDHGCPSCGSCSGMFTANSMNCLCEVLGLALPGNGSILAIDPRREELIKQAAEKLKILI
ERDIKPRDIVTEEAIDDAFALDMAMGGSTNTVLHTLALAQEAGLDYDMNRIDAVSRRVPHLCKVSPASNWHMEDIDRAGG
ISAILKEMSRKEGVLHLDRITATGQTLRENIAEAEIKDKEVIHSLENPHSEEGGLRILKGNLAKDGAVIKSGATEVKRFE
GPCVIFNSQDEALAGIMLGKVKKGDVVVIRYEGPRGGPGMPEMLAPTSAIAGMGLGAEVALLTDGRFSGASRGISVGHIS
PEAAAGGMIALLEQGDIVCIDVEERLLEVRVSDEELEKRKKEWKRPEPKVKTGWLGRYAQMVTSANTGAVLKIPNFD
>Mature_557_residues
MRSDMIKKGFDKAPHRSLLKATGLKDEDFDKPFIAICNSFIEIIPGHKHLNEFGKLVKEAVRAAGMVPFEFNTIGVDDGI
AMGHIGMRYSLPSREIIADSVETVVNAHWFDGMICIPNCDKITPGMMMAALRINIPTVFVSGGPMAAGKTSKGEVVDLSS
VFEGVGAYQSGKISEEELKDIEDHGCPSCGSCSGMFTANSMNCLCEVLGLALPGNGSILAIDPRREELIKQAAEKLKILI
ERDIKPRDIVTEEAIDDAFALDMAMGGSTNTVLHTLALAQEAGLDYDMNRIDAVSRRVPHLCKVSPASNWHMEDIDRAGG
ISAILKEMSRKEGVLHLDRITATGQTLRENIAEAEIKDKEVIHSLENPHSEEGGLRILKGNLAKDGAVIKSGATEVKRFE
GPCVIFNSQDEALAGIMLGKVKKGDVVVIRYEGPRGGPGMPEMLAPTSAIAGMGLGAEVALLTDGRFSGASRGISVGHIS
PEAAAGGMIALLEQGDIVCIDVEERLLEVRVSDEELEKRKKEWKRPEPKVKTGWLGRYAQMVTSANTGAVLKIPNFD

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=606, Percent_Identity=47.8547854785479, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1788157, Length=540, Percent_Identity=30.7407407407407, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI2367371, Length=526, Percent_Identity=31.3688212927757, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786464, Length=494, Percent_Identity=31.3765182186235, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322476, Length=545, Percent_Identity=42.0183486238532, Blast_Score=424, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 60057; Mature: 60057

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDMIKKGFDKAPHRSLLKATGLKDEDFDKPFIAICNSFIEIIPGHKHLNEFGKLVKEA
CCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VRAAGMVPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADSVETVVNAHWFDGMICIPNCD
HHHCCCCCEEEEEECCCCCCEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
KITPGMMMAALRINIPTVFVSGGPMAAGKTSKGEVVDLSSVFEGVGAYQSGKISEEELKD
CCCHHHHHHHEEEECCEEEECCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCHHHHHH
IEDHGCPSCGSCSGMFTANSMNCLCEVLGLALPGNGSILAIDPRREELIKQAAEKLKILI
HHHCCCCCCCCCCCCEECCHHHHHHHHHHEECCCCCCEEEECCHHHHHHHHHHHHHHHHH
ERDIKPRDIVTEEAIDDAFALDMAMGGSTNTVLHTLALAQEAGLDYDMNRIDAVSRRVPH
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
LCKVSPASNWHMEDIDRAGGISAILKEMSRKEGVLHLDRITATGQTLRENIAEAEIKDKE
CEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCEEEEHHHHCHHHHHHHHHHHHHHHHH
VIHSLENPHSEEGGLRILKGNLAKDGAVIKSGATEVKRFEGPCVIFNSQDEALAGIMLGK
HHHHHCCCCCCCCCEEEEECCCCCCCCEEECCCHHHHHCCCCEEEECCCCHHHHHHHHCC
VKKGDVVVIRYEGPRGGPGMPEMLAPTSAIAGMGLGAEVALLTDGRFSGASRGISVGHIS
CCCCCEEEEEECCCCCCCCCCHHHCCHHHHHCCCCCCEEEEEECCCCCCCCCCEEEECCC
PEAAAGGMIALLEQGDIVCIDVEERLLEVRVSDEELEKRKKEWKRPEPKVKTGWLGRYAQ
HHHHCCCEEEEEECCCEEEEEHHHHHEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MVTSANTGAVLKIPNFD
HHHCCCCCEEEECCCCC
>Mature Secondary Structure
MRSDMIKKGFDKAPHRSLLKATGLKDEDFDKPFIAICNSFIEIIPGHKHLNEFGKLVKEA
CCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VRAAGMVPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADSVETVVNAHWFDGMICIPNCD
HHHCCCCCEEEEEECCCCCCEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
KITPGMMMAALRINIPTVFVSGGPMAAGKTSKGEVVDLSSVFEGVGAYQSGKISEEELKD
CCCHHHHHHHEEEECCEEEECCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCHHHHHH
IEDHGCPSCGSCSGMFTANSMNCLCEVLGLALPGNGSILAIDPRREELIKQAAEKLKILI
HHHCCCCCCCCCCCCEECCHHHHHHHHHHEECCCCCCEEEECCHHHHHHHHHHHHHHHHH
ERDIKPRDIVTEEAIDDAFALDMAMGGSTNTVLHTLALAQEAGLDYDMNRIDAVSRRVPH
HCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
LCKVSPASNWHMEDIDRAGGISAILKEMSRKEGVLHLDRITATGQTLRENIAEAEIKDKE
CEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCEEEEHHHHCHHHHHHHHHHHHHHHHH
VIHSLENPHSEEGGLRILKGNLAKDGAVIKSGATEVKRFEGPCVIFNSQDEALAGIMLGK
HHHHHCCCCCCCCCEEEEECCCCCCCCEEECCCHHHHHCCCCEEEECCCCHHHHHHHHCC
VKKGDVVVIRYEGPRGGPGMPEMLAPTSAIAGMGLGAEVALLTDGRFSGASRGISVGHIS
CCCCCEEEEEECCCCCCCCCCHHHCCHHHHHCCCCCCEEEEEECCCCCCCCCCEEEECCC
PEAAAGGMIALLEQGDIVCIDVEERLLEVRVSDEELEKRKKEWKRPEPKVKTGWLGRYAQ
HHHHCCCEEEEEECCCEEEEEHHHHHEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
MVTSANTGAVLKIPNFD
HHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA