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The map label for this gene is rfbD [H]

Identifier: 218232620

GI number: 218232620

Start: 1236279

End: 1237019

Strand: Direct

Name: rfbD [H]

Synonym: BCB4264_A1270

Alternate gene names: 218232620

Gene position: 1236279-1237019 (Clockwise)

Preceding gene: 218232660

Following gene: 218231805

Centisome position: 22.81

GC content: 37.92

Gene sequence:

>741_bases
GTGACAAATATATCCCGAATACAACAAGTGGTACAAGAAATAAAACCACATATAATTATTCATTGTGCTGCCTATACGAA
AGTAGATCATGCGGAGAAAGAACAGGATCTTGCATATAGAATAAATGCAATCGGAGCTCGAAATGTAGCGGTTGCTTCAC
AATTAGTAGGGGCGAAGCTAGTTTATATTAGTACTGATTATGTATTTCAAGGCGCCAGACCAGATGGATACGACGAATTT
CATAGTCCAGCACCGATAAATATATATGGGGCCTCTAAGTATGCGGGGGAGCAGTTCGTCAAAGAGTTACATAATAAATA
TTTTATCGTTCGCACATCGTGGCTATATGGTAAATATGGGAATAATTTTGTGAAAACGATGATGCGATTAGGAAAAGAAA
GAGATGAAATATCTGTTGTAGCGGATCAAGTTGGCTCTCCTACGTATGTGGCGGATTTGAATATGGTTATTAATAAGCTC
ATTCATACTTCTTTATACGGTACATACCACGTATCAAACAGAGGCTCATGCTCTTGGTTTGAATTTGCACAAAAGATATT
TTCGTACGCCAATATGAAAGTGCATGTATTACCTGTTTCAACGGAAGAATTCGGGGCTGCGGCTGCGAGGCCCAAATATT
CTATTTTTCAACATAACATGCTAAGATTAAATGGTTTTTCACAAATGCCGTCTTGGGAAGAAGGATTAGAGCGGTTTTTT
ATAGAAACCAAAAGTCATTAA

Upstream 100 bases:

>100_bases
TATCATAACAGGAGCAAACGGTCAATTAGGAAAGCAACTATTTGAAGAGTTAGACTCTGAAGAATATGATATATATCCAT
TTGATAAAAAGTTATTAGAT

Downstream 100 bases:

>100_bases
CAAATTTCTGTTAATGACTTTTTTGTTTGCCTTTAAGCGGTTTTATGGTACTATAATTATAGTATCAGGTACTAATAACA
AGTATAAGTATTTCTGGGAG

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTNISRIQQVVQEIKPHIIIHCAAYTKVDHAEKEQDLAYRINAIGARNVAVASQLVGAKLVYISTDYVFQGARPDGYDEF
HSPAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYGNNFVKTMMRLGKERDEISVVADQVGSPTYVADLNMVINKL
IHTSLYGTYHVSNRGSCSWFEFAQKIFSYANMKVHVLPVSTEEFGAAAARPKYSIFQHNMLRLNGFSQMPSWEEGLERFF
IETKSH

Sequences:

>Translated_246_residues
MTNISRIQQVVQEIKPHIIIHCAAYTKVDHAEKEQDLAYRINAIGARNVAVASQLVGAKLVYISTDYVFQGARPDGYDEF
HSPAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYGNNFVKTMMRLGKERDEISVVADQVGSPTYVADLNMVINKL
IHTSLYGTYHVSNRGSCSWFEFAQKIFSYANMKVHVLPVSTEEFGAAAARPKYSIFQHNMLRLNGFSQMPSWEEGLERFF
IETKSH
>Mature_245_residues
TNISRIQQVVQEIKPHIIIHCAAYTKVDHAEKEQDLAYRINAIGARNVAVASQLVGAKLVYISTDYVFQGARPDGYDEFH
SPAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYGNNFVKTMMRLGKERDEISVVADQVGSPTYVADLNMVINKLI
HTSLYGTYHVSNRGSCSWFEFAQKIFSYANMKVHVLPVSTEEFGAAAARPKYSIFQHNMLRLNGFSQMPSWEEGLERFFI
ETKSH

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=262, Percent_Identity=29.3893129770992, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI11034825, Length=262, Percent_Identity=29.3893129770992, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788352, Length=257, Percent_Identity=35.7976653696498, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 28019; Mature: 27888

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNISRIQQVVQEIKPHIIIHCAAYTKVDHAEKEQDLAYRINAIGARNVAVASQLVGAKL
CCCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEE
VYISTDYVFQGARPDGYDEFHSPAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYG
EEEEECEEEECCCCCCCHHHCCCCCEEEEECCHHHHHHHHHHHCCCEEEEEEEEEECCCC
NNFVKTMMRLGKERDEISVVADQVGSPTYVADLNMVINKLIHTSLYGTYHVSNRGSCSWF
CHHHHHHHHHCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCEEEEECCCCCCHHH
EFAQKIFSYANMKVHVLPVSTEEFGAAAARPKYSIFQHNMLRLNGFSQMPSWEEGLERFF
HHHHHHHHHCCCEEEEEECCHHHHCCHHCCCCHHHHHCCCEEECCCCCCCCHHHHHHHHH
IETKSH
HHCCCC
>Mature Secondary Structure 
TNISRIQQVVQEIKPHIIIHCAAYTKVDHAEKEQDLAYRINAIGARNVAVASQLVGAKL
CCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEE
VYISTDYVFQGARPDGYDEFHSPAPINIYGASKYAGEQFVKELHNKYFIVRTSWLYGKYG
EEEEECEEEECCCCCCCHHHCCCCCEEEEECCHHHHHHHHHHHCCCEEEEEEEEEECCCC
NNFVKTMMRLGKERDEISVVADQVGSPTYVADLNMVINKLIHTSLYGTYHVSNRGSCSWF
CHHHHHHHHHCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCEEEEECCCCCCHHH
EFAQKIFSYANMKVHVLPVSTEEFGAAAARPKYSIFQHNMLRLNGFSQMPSWEEGLERFF
HHHHHHHHHCCCEEEEEECCHHHHCCHHCCCCHHHHHCCCEEECCCCCCCCHHHHHHHHH
IETKSH
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]