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The map label for this gene is rnjA [H]

Identifier: 218232605

GI number: 218232605

Start: 1600402

End: 1602075

Strand: Direct

Name: rnjA [H]

Synonym: BCB4264_A1676

Alternate gene names: 218232605

Gene position: 1600402-1602075 (Clockwise)

Preceding gene: 218234469

Following gene: 218235264

Centisome position: 29.53

GC content: 32.97

Gene sequence:

>1674_bases
TTGAAGGCATTAAAAAATAACTTGTCTATTGTTGCTTTAGGTGGAGTAAATGAAATAGGAAAAAATATGTATGCTATCCA
ATATGAAAATGATATTGTCGTCATTGATTGTGGATCTAAATTTCCAGATGAAAGTTTATTAGGGATTGATTTAATAATTC
CAGATGTCACATATTTGCAAGAAAATAAAGAAAAAATTCGCGGTTTAATAGTAACGCACGGGCATGAGGACCATATTGGC
GGAATACCATATTTTTTAAAACAACTAAATGTACCAATCTATGCAACAAAGTTAACGTTAGGTTTAATTGAAATTAAATT
AAAGGAACACAATCTTCAAAATGATACGGAATTAATCGTTATTCATTCAGAATCAGAAATTGATTGTGGTTCTATTAAGG
CAACTTTTTTTAAAACGAACCACAGTATTCCGGATTGTCTCGGTATTGTTTTTCATACACCAGAAGGTACTGTAGTACAT
ACAGGTGATTTTAAATTTGATTTAACACCAGTAAATAATCAACATCCTGATATTCATAAAATGGCTAAAATAGGAAGTGA
AGGTGTACTAGCTTTATTATCTGAAAGCACAAATGCAGAACGCCCAGGTTTTACTCCATCAGAAAGGTCAGTAGGAGAAC
GGATAGAGGAAATATTTATGAAAGCAAATAGAAAAGTAATTATTTCTACGTTTGCTTCTAATGTGAATCGTGTTCAGCAA
ATAGTTGAAGCATGTATAAAAACAAATCGAAAACTAGCTTTGCTAGGGAGAAGTATGGTAAATGTAGTAGAGGTTGCTCT
AGAGAAAGGTTATTTACATATTCCAGAAGGTATGTTGATTGAAGCAAATGAGGTAAATCGTTTAGATCCAAAGCAGGTTG
CAATACTTTGTACAGGAAGTCAAGGAGAACCAATGGCAGCTTTAGCGCGTTTAGCGAGTGGAAACTATAGACAAGTGGAT
ATTTTACCAGAAGATACGGTTATTATAGCCGCGACCCCTATCCCAGGAAATGAACGTAACGTTTCTCGAATTATCGATAA
TTTATTTGCATTAGGAGCAAAGGTGATTTACGGATCAGGTAGTTCTACTGGAGTGCATGTATCCGGTCATGCATATCAAG
AAGAGTTGAAGTTAATGCTTACATTAATGAAGCCAAAATATTTTATTCCAATTCATGGAGAGTTTAGAATGCTACATCAC
CATAGTTTGTTAGCAGAATCAATTGGTATTGAAAAGGAAAATATCTTTATTGTACGTAATGGAGATGTTGTAGATATAAG
TAATGAAGTGGCAATTCAATCTAGAAGAATACAGGCAGGAAATATATATGTAGATGGATTAGGAATTGGAGATGTTGGAA
ATGCATTATTACGTGATCGTAAACAACTTTCAGAAGATGGAATGCTCGTTATAGTTATTACTTTTAATAAAGTGGATGGA
GAAATAATTTCAGGTCCAGATATTATTTCTCGTGGATTTGTCTATGTCCGTGATTCAGAAGAATTTCTAAGAGAATTAAA
TAAATTAGCTGTTATTACGATTAATAATTTAAAGAAAGAGAATGTGAATAGCTGGGGTATTTTGAAAAGGGAGGTAAGAG
AAGCATTAGGGAAGTATATATATACAAATACAAAACGAAAGCCGATGATTCTTCCTATAATAATAGAGGTTTAA

Upstream 100 bases:

>100_bases
TTTTATATGTATAAAATAGTATGAAGTCCTTTTCCATTACAAATAATAAATTCTACATACTATTTCACGAACTATAACTG
TGAAAGGGAGGATTTATAAT

Downstream 100 bases:

>100_bases
TAGTAAAAAAGAATTCTCGTATGGGAATTCTTTTTTGTTTTGAAGAGAAAATGAACATATAATGAAATAAATAGAAATGG
AAGGAAATAAAAGATGTTAT

Product: RNA-metabolising metallo-beta-lactamase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MKALKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQENKEKIRGLIVTHGHEDHIG
GIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVIHSESEIDCGSIKATFFKTNHSIPDCLGIVFHTPEGTVVH
TGDFKFDLTPVNNQHPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLHIPEGMLIEANEVNRLDPKQVAILCTGSQGEPMAALARLASGNYRQVD
ILPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSGSSTGVHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHH
HSLLAESIGIEKENIFIVRNGDVVDISNEVAIQSRRIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
EIISGPDIISRGFVYVRDSEEFLRELNKLAVITINNLKKENVNSWGILKREVREALGKYIYTNTKRKPMILPIIIEV

Sequences:

>Translated_557_residues
MKALKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQENKEKIRGLIVTHGHEDHIG
GIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVIHSESEIDCGSIKATFFKTNHSIPDCLGIVFHTPEGTVVH
TGDFKFDLTPVNNQHPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLHIPEGMLIEANEVNRLDPKQVAILCTGSQGEPMAALARLASGNYRQVD
ILPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSGSSTGVHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHH
HSLLAESIGIEKENIFIVRNGDVVDISNEVAIQSRRIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
EIISGPDIISRGFVYVRDSEEFLRELNKLAVITINNLKKENVNSWGILKREVREALGKYIYTNTKRKPMILPIIIEV
>Mature_557_residues
MKALKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQENKEKIRGLIVTHGHEDHIG
GIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIVIHSESEIDCGSIKATFFKTNHSIPDCLGIVFHTPEGTVVH
TGDFKFDLTPVNNQHPDIHKMAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLHIPEGMLIEANEVNRLDPKQVAILCTGSQGEPMAALARLASGNYRQVD
ILPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSGSSTGVHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHH
HSLLAESIGIEKENIFIVRNGDVVDISNEVAIQSRRIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
EIISGPDIISRGFVYVRDSEEFLRELNKLAVITINNLKKENVNSWGILKREVREALGKYIYTNTKRKPMILPIIIEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61772; Mature: 61772

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQ
CCCCCCCCCEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
ENKEKIRGLIVTHGHEDHIGGIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIV
CCHHHEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEE
IHSESEIDCGSIKATFFKTNHSIPDCLGIVFHTPEGTVVHTGDFKFDLTPVNNQHPDIHK
EECCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHH
MAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHH
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLHIPEGMLIEANEVNRLDPKQVAILCTGS
HHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCEEEEEEECC
QGEPMAALARLASGNYRQVDILPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSG
CCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECC
SSTGVHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHHHSLLAESIGIEKENIFIVRN
CCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEC
GDVVDISNEVAIQSRRIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
CCEEECCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
EIISGPDIISRGFVYVRDSEEFLRELNKLAVITINNLKKENVNSWGILKREVREALGKYI
CEECCCCHHHCCEEEEECCHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHE
YTNTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC
>Mature Secondary Structure
MKALKNNLSIVALGGVNEIGKNMYAIQYENDIVVIDCGSKFPDESLLGIDLIIPDVTYLQ
CCCCCCCCCEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHH
ENKEKIRGLIVTHGHEDHIGGIPYFLKQLNVPIYATKLTLGLIEIKLKEHNLQNDTELIV
CCHHHEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEE
IHSESEIDCGSIKATFFKTNHSIPDCLGIVFHTPEGTVVHTGDFKFDLTPVNNQHPDIHK
EECCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCEEECCCEEEEEEECCCCCCCHHH
MAKIGSEGVLALLSESTNAERPGFTPSERSVGERIEEIFMKANRKVIISTFASNVNRVQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHH
IVEACIKTNRKLALLGRSMVNVVEVALEKGYLHIPEGMLIEANEVNRLDPKQVAILCTGS
HHHHHHHCCCEEEEHHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCEEEEEEECC
QGEPMAALARLASGNYRQVDILPEDTVIIAATPIPGNERNVSRIIDNLFALGAKVIYGSG
CCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECC
SSTGVHVSGHAYQEELKLMLTLMKPKYFIPIHGEFRMLHHHSLLAESIGIEKENIFIVRN
CCCCEEEECHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHHHHHHCCCCCCEEEEEC
GDVVDISNEVAIQSRRIQAGNIYVDGLGIGDVGNALLRDRKQLSEDGMLVIVITFNKVDG
CCEEECCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
EIISGPDIISRGFVYVRDSEEFLRELNKLAVITINNLKKENVNSWGILKREVREALGKYI
CEECCCCHHHCCEEEEECCHHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHE
YTNTKRKPMILPIIIEV
EECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]