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The map label for this gene is nplT [H]

Identifier: 218232441

GI number: 218232441

Start: 1179414

End: 1180709

Strand: Direct

Name: nplT [H]

Synonym: BCB4264_A1211

Alternate gene names: 218232441

Gene position: 1179414-1180709 (Clockwise)

Preceding gene: 218230952

Following gene: 218234097

Centisome position: 21.76

GC content: 34.95

Gene sequence:

>1296_bases
GTGGGAAAAATACGGACTAGGAAACTATTCATTTGTTTTTGCTTAGCTGTTGTTTTGTTTGTACCAATACATACTTTTGC
AGACGAAAAAAGAGAGTGGCGAGATGAAGTTATATACTCCATTATGATTGATCGCTTCAATAATGGAGAACCGAAAAATG
ACAAGCAGTTAGAAGTTGGTAATTTAGAAGGGTATCAAGGCGGGGATATAAGAGGAATTATAAAAAGGTTGGATTACATA
AAAGAAATGGGTTTTACTACCATTATGCTTTCGCCGCTTTTTGAAAGTGGAAAGTACGATGGATTAGACGTGCGAAATTT
TAAAAAGGTAAATGAACATTTCGGGACAGACAATGATGTGAAAGAACTTGTAAAAGAAGCTCATGCAAAAGGAATGAAAG
TTGTATTTCAATTTCCGCTTGGAGAAAACGAACGACAGGTAATTGACGCAATGAAATGGTGGATAAAAGAAGTTGATTTA
GACGGAAGTTATGTTATACATAGTGAAAAAAAGCCGCGTTCCTTTTGGGATAACGCGCAAAAAGATATGCAAGTGATAAA
GAAAGATTTTCGTATTATGACAAAGGAGGATAGCGAATACAACGAAAAAATAGTAGAATCGTTTTCCGAAGCGGATGTAT
CGGTGAAATCGTTATACGATGTGAGTAAAAAAGAAGGGGAATTTGTTACGTTTTTAGATGATCAAGAAACAAAAAGATAT
GCGCGTATTGCAAAAGAAAATATGTATTATCCGCCATCACGTTTAAAACTGGCGCTTACATATTTGTTAACATCACCTGG
GATTCCGAATTTTTATTACGGAACTGAAATTGCATTAGATGGAGGAAGTGTACCCGATAATAGACGATTAATGGATTTTA
AGTCAGACGAAAAATTTATGCAGCACATAACAAAACTAGGTGAACTTAGGCAGGCGAGACCGTCTTTACGACGCGGTACG
TTTGAGCTTCTGTATGATAAGAGCGGGATGAGTATACTAAAACGAAAATATAAAGATGAAGTAACTTTAGTAGCAATTAA
TAATACGAAAGAAACACAAAAAGTCTCCTTACCTGCAAGTGAAATTGGTGAAAAACAAGAGTTAAGAGGATTGTTGCAGG
ATGAAATTATAAGAGAGGAAAATGGAAAATTTTATCTCGTTTTAAAGCGTGAAGAATCAAACGTGTATAAAGTTAACGGA
GAAACAGGTGTGAATTGGTTATTTATCTCCTTAATCGTTGGTGTGAATGTATTATTTATTGCGTTTTTAATTGCAGTTAA
AAGGAAACGTAGATGA

Upstream 100 bases:

>100_bases
AACCTAACATGAATTATTTTGTGAGGATTAAAATCGACATATGCGCTTCATTCAGAAAATTGGTATAATATTCTTACAAT
TAAAAACTTGGGGGATGCGT

Downstream 100 bases:

>100_bases
GATGAGAAGTTACTCACTCTATGTTCATGTCTTCTTATTGGATTTGTAAGTTTGTTCGGTGGGGCCAGGTTAGGGAGAAA
GTCTTCTTCCTAGCCTGTTT

Product: alpha-amylase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGKIRTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVGNLEGYQGGDIRGIIKRLDYI
KEMGFTTIMLSPLFESGKYDGLDVRNFKKVNEHFGTDNDVKELVKEAHAKGMKVVFQFPLGENERQVIDAMKWWIKEVDL
DGSYVIHSEKKPRSFWDNAQKDMQVIKKDFRIMTKEDSEYNEKIVESFSEADVSVKSLYDVSKKEGEFVTFLDDQETKRY
ARIAKENMYYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGSVPDNRRLMDFKSDEKFMQHITKLGELRQARPSLRRGT
FELLYDKSGMSILKRKYKDEVTLVAINNTKETQKVSLPASEIGEKQELRGLLQDEIIREENGKFYLVLKREESNVYKVNG
ETGVNWLFISLIVGVNVLFIAFLIAVKRKRR

Sequences:

>Translated_431_residues
MGKIRTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVGNLEGYQGGDIRGIIKRLDYI
KEMGFTTIMLSPLFESGKYDGLDVRNFKKVNEHFGTDNDVKELVKEAHAKGMKVVFQFPLGENERQVIDAMKWWIKEVDL
DGSYVIHSEKKPRSFWDNAQKDMQVIKKDFRIMTKEDSEYNEKIVESFSEADVSVKSLYDVSKKEGEFVTFLDDQETKRY
ARIAKENMYYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGSVPDNRRLMDFKSDEKFMQHITKLGELRQARPSLRRGT
FELLYDKSGMSILKRKYKDEVTLVAINNTKETQKVSLPASEIGEKQELRGLLQDEIIREENGKFYLVLKREESNVYKVNG
ETGVNWLFISLIVGVNVLFIAFLIAVKRKRR
>Mature_430_residues
GKIRTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVGNLEGYQGGDIRGIIKRLDYIK
EMGFTTIMLSPLFESGKYDGLDVRNFKKVNEHFGTDNDVKELVKEAHAKGMKVVFQFPLGENERQVIDAMKWWIKEVDLD
GSYVIHSEKKPRSFWDNAQKDMQVIKKDFRIMTKEDSEYNEKIVESFSEADVSVKSLYDVSKKEGEFVTFLDDQETKRYA
RIAKENMYYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGSVPDNRRLMDFKSDEKFMQHITKLGELRQARPSLRRGTF
ELLYDKSGMSILKRKYKDEVTLVAINNTKETQKVSLPASEIGEKQELRGLLQDEIIREENGKFYLVLKREESNVYKVNGE
TGVNWLFISLIVGVNVLFIAFLIAVKRKRR

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=128, Percent_Identity=26.5625, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786604, Length=154, Percent_Identity=31.1688311688312, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790687, Length=105, Percent_Identity=31.4285714285714, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=112, Percent_Identity=31.25, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324416, Length=108, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=108, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322021, Length=108, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321731, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319776, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583745, Length=162, Percent_Identity=30.2469135802469, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24586587, Length=72, Percent_Identity=43.0555555555556, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 50145; Mature: 50014

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIRTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
NLEGYQGGDIRGIIKRLDYIKEMGFTTIMLSPLFESGKYDGLDVRNFKKVNEHFGTDNDV
CCCCCCCCCHHHHHHHHHHHHHCCCHHEEHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHH
KELVKEAHAKGMKVVFQFPLGENERQVIDAMKWWIKEVDLDGSYVIHSEKKPRSFWDNAQ
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHH
KDMQVIKKDFRIMTKEDSEYNEKIVESFSEADVSVKSLYDVSKKEGEFVTFLDDQETKRY
HHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCHHHHHH
ARIAKENMYYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGSVPDNRRLMDFKSDEKFM
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHH
QHITKLGELRQARPSLRRGTFELLYDKSGMSILKRKYKDEVTLVAINNTKETQKVSLPAS
HHHHHHHHHHHHCHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCEEECCHH
EIGEKQELRGLLQDEIIREENGKFYLVLKREESNVYKVNGETGVNWLFISLIVGVNVLFI
HHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHH
AFLIAVKRKRR
HHHHHHHHCCC
>Mature Secondary Structure 
GKIRTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
NLEGYQGGDIRGIIKRLDYIKEMGFTTIMLSPLFESGKYDGLDVRNFKKVNEHFGTDNDV
CCCCCCCCCHHHHHHHHHHHHHCCCHHEEHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHH
KELVKEAHAKGMKVVFQFPLGENERQVIDAMKWWIKEVDLDGSYVIHSEKKPRSFWDNAQ
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHH
KDMQVIKKDFRIMTKEDSEYNEKIVESFSEADVSVKSLYDVSKKEGEFVTFLDDQETKRY
HHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCHHHHHH
ARIAKENMYYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGSVPDNRRLMDFKSDEKFM
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHH
QHITKLGELRQARPSLRRGTFELLYDKSGMSILKRKYKDEVTLVAINNTKETQKVSLPAS
HHHHHHHHHHHHCHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCEEECCHH
EIGEKQELRGLLQDEIIREENGKFYLVLKREESNVYKVNGETGVNWLFISLIVGVNVLFI
HHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHH
AFLIAVKRKRR
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]