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The map label for this gene is dctA [H]

Identifier: 218232428

GI number: 218232428

Start: 3616009

End: 3617271

Strand: Reverse

Name: dctA [H]

Synonym: BCB4264_A3697

Alternate gene names: 218232428

Gene position: 3617271-3616009 (Counterclockwise)

Preceding gene: 218233122

Following gene: 218233091

Centisome position: 66.75

GC content: 36.1

Gene sequence:

>1263_bases
ATGAGAATCGTAAAGAATTTAACATTCCAAGTTATTGTGGCTATTATTTGTGGTATTGCAGTAGGGGCAATTTGGCCGAG
TGTTGGACAACAAATGAAGCCAATTGGTGAAACATTCATCAATATGATTAAAATGGTTATTGCACCGATTATCTTTTTAA
CAATTGTGCTAGGTATTGCAAGTATGGGAAGTATGAAAAAGGTAGGCCGTGTTGGTGGGAAGGCACTATTATATTTTGAA
ATTGTTACAACAGCGGCTTTAATTATTGGTATCATCGTAGCGAATGTTGTACGTCCAGGAGATGGATTAGATCCCTCAAA
ACTAAAGGGCGGAGATGTTTCACAATATGTTCAAAGCGGACAAGAAATGAAATGGATGGATTTCTTTTTACATATCGTGC
CGTCTAACATGTTTGAAGCATTTTCCAAAGGTGATATTTTACAAGTTCTGTTCTTCTCCATTTTATTCGGCGCAGGGTTA
ACGATGTTAGGGAAAAATGGACAACCGGTTATTGACTTTTTTGAAAGATTATCGAAAGTATTTTTTAACATTTTATCAAT
TGTAATGAAATTAGCACCGGTTGGAGCATTCGGTGGGATGGCATTTACAATTGGGAAGTATGGTTTAAGCACGCTCATAC
CACTTGGTAAATTAATGATTTGTGTATATGCGACAATGGCATTATTTGTTTTTATTGTTTTAAATTTTATTTGTAAACTG
TACAAATTTAGTTTGTGGAAATATTTAGCGCATATTAAAGAAGAATTACTTATTGTTCTCGGGACATCATCGTCAGAATC
AGTACTGCCGCGGATGATGACAAAGATGGAAGACTTCGGGTGTTCGAAGCCGGTAGTAGGTTTAGTCATTCCAACAGGTT
ACTCTTTTAACTTAGATGGAACAACGATTTATTTATCAATGGCAACTATATTTTTAGCGCAAGTTTTTCACGTCGATCTT
TCACTAGGTCAACAATTAACTATAATAGCTATTTTGTTAGTTACATCTAAAGGAGCAGCAGCAGTAACCGGCGGGGGATT
CATTGTACTAGCATCTACTCTATCTGCAATGAATGTTATCCCATTAGAAGGTTTAGCGTTATTACTTGGTGTAGATCGTT
TCATGTCAGAAGCACGTGCTATCGTTAATTTAATTGGTAACGGTATTGCAACTGTAGTTGTTGCGAAAAGTGAAAATGAA
TTTGATGATGAGAAGTATACGAGAGTAGTAGAGGAAATGAAAAGAGAGAAAATGGTCGGGTAA

Upstream 100 bases:

>100_bases
ATAGTTTAAGTTAAATAACAGGGGGGCTATTACATAGAATATATCATTAGCAATTATTGAAATAGTATGGGAGAAATGAA
AATACAGGGGAGTATCAAAA

Downstream 100 bases:

>100_bases
AAAGTCAGCTTTGCTGACTTTTTTATTTTTGTAGAAAAGATGTTTTTAGATATATGCATTATTTATTAGCAATAATTAAC
AAGTATTCAAGTGGTAAATG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQQMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFSKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDDEKYTRVVEEMKREKMVG

Sequences:

>Translated_420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQQMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFSKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDDEKYTRVVEEMKREKMVG
>Mature_420_residues
MRIVKNLTFQVIVAIICGIAVGAIWPSVGQQMKPIGETFINMIKMVIAPIIFLTIVLGIASMGSMKKVGRVGGKALLYFE
IVTTAALIIGIIVANVVRPGDGLDPSKLKGGDVSQYVQSGQEMKWMDFFLHIVPSNMFEAFSKGDILQVLFFSILFGAGL
TMLGKNGQPVIDFFERLSKVFFNILSIVMKLAPVGAFGGMAFTIGKYGLSTLIPLGKLMICVYATMALFVFIVLNFICKL
YKFSLWKYLAHIKEELLIVLGTSSSESVLPRMMTKMEDFGCSKPVVGLVIPTGYSFNLDGTTIYLSMATIFLAQVFHVDL
SLGQQLTIIAILLVTSKGAAAVTGGGFIVLASTLSAMNVIPLEGLALLLGVDRFMSEARAIVNLIGNGIATVVVAKSENE
FDDEKYTRVVEEMKREKMVG

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=449, Percent_Identity=24.7216035634744, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI40254478, Length=475, Percent_Identity=24.6315789473684, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI21314632, Length=431, Percent_Identity=25.5220417633411, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI5032093, Length=421, Percent_Identity=26.3657957244656, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI66773030, Length=420, Percent_Identity=26.4285714285714, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI223468564, Length=283, Percent_Identity=28.6219081272085, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI223468566, Length=283, Percent_Identity=28.6219081272085, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI194239697, Length=430, Percent_Identity=24.6511627906977, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI4827012, Length=277, Percent_Identity=27.0758122743682, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI262359914, Length=376, Percent_Identity=24.2021276595745, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI301601644, Length=147, Percent_Identity=29.9319727891156, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789947, Length=410, Percent_Identity=55.609756097561, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1790514, Length=423, Percent_Identity=40.8983451536643, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1789473, Length=319, Percent_Identity=22.884012539185, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=425, Percent_Identity=27.0588235294118, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71983106, Length=417, Percent_Identity=25.4196642685851, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983099, Length=417, Percent_Identity=25.4196642685851, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193206505, Length=420, Percent_Identity=26.4285714285714, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71996953, Length=413, Percent_Identity=25.90799031477, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI193206654, Length=437, Percent_Identity=24.7139588100687, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=436, Percent_Identity=23.8532110091743, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24583025, Length=400, Percent_Identity=24.5, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI17137668, Length=400, Percent_Identity=24.5, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24583023, Length=400, Percent_Identity=24.5, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI281360483, Length=419, Percent_Identity=24.1050119331742, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137666, Length=419, Percent_Identity=24.1050119331742, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281360481, Length=419, Percent_Identity=24.1050119331742, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45538; Mature: 45538

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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>Mature Secondary Structure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PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]