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The map label for this gene is ykfB [H]

Identifier: 218232310

GI number: 218232310

Start: 2749355

End: 2750416

Strand: Reverse

Name: ykfB [H]

Synonym: BCB4264_A2863

Alternate gene names: 218232310

Gene position: 2750416-2749355 (Counterclockwise)

Preceding gene: 218233132

Following gene: 218231988

Centisome position: 50.75

GC content: 38.89

Gene sequence:

>1062_bases
ATGAACAAAATGAAAATTACAGATGTAAAAGTAAATCGTAGACGTGTAAAACTGCATACACCGTTTAAAACCGCGCTTCG
TACTGTAACCGAAATTGAAAGTATAGATGTTTATATTCATACAGACGAAGGAGTGATTGGTAAGGGGGCTGCAGCCGCAA
CGCCAGTTATTACGGGGGATTTCACCAGCGGAATGGAAGAAGCGATTTTAGGGCCGATGCGTTCATGTTTAATTGGTCAA
AATATCATTCAATTTCAGCAGTTACTACAGCGCATTCAAATGAGTTGTATTGGAAATTCAAGTGCGAAAGCAGCGGTAGA
TATCGCTTTATATGATGTATATTGCCAATATCAAAATGTGCCGTTATATGCATTGTTAGGCGGGAAGAAAGAAATTCATA
CCGATATTACAGTGAGTGTGGATGAGCCTGTATTAATGGCAAAAGAAGCGAAGAAACATATAGAAAAAGGATTTCAAACA
TTAAAGATTAAAGTGGGTAAAGAGGCGCATTTAGATTTGGAACGTATTGAGGCAATTCGAAATGTGGTACCAAGAAATAC
GACATTACGTTTAGATGCGAATCAAGGTTGGAGTCCAAAAGAAGCGGTCTCTATCATTCAAGAGATGGAAAATCGTAATT
TAAATATAGAATTTATTGAACAACCAGTACACGCGAAAGATTGGGATGGGTTAAAGTACGTCAAAGATCGTGTGCAAACG
CCAATTATGGCCGATGAAAGTATATTTTCAGCAAGTGACGCATTAAAGCTCGTTCAAGGAAGATATGCAGACTTCATTAA
TATTAAATTAATGAAATGTGGTGGCATACGTGAAGCATGGCGTATTGCTGATATCGCAGAAACAGCTGGTGTGAAGTGTA
TGGTGGGCAGCATGATGGAGTCTTCACTTTCCGTTAGTGCTGTTGCGCATTTAGCGGCAGCGCATCCTAATATTCATTAT
TTTGATCTTGATGCACCGCTTTGGTTAATGGAAGAGCCAGAAGGAATGACTTATTCTGGATCAAAGGTAAACCTTCAATC
GACAGTGAATAGTAAATCTTAG

Upstream 100 bases:

>100_bases
GTAGAGAGATAGGAGAGTAAATGCCCAATGGGTTTACCTAATAATCCGTGGGTATAAATCTCCGGTGGATTAAAATTTCA
CTTCATTGGAGAACGAAAGG

Downstream 100 bases:

>100_bases
GAGAAGAGACAAGTAAACTATCTTTTAAGGGGGATATATATGAAAAAAGTAGGAACTGCATTTTTAACAACTTTATTTAT
ATTTTCATCGTTTACATCGG

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNKMKITDVKVNRRRVKLHTPFKTALRTVTEIESIDVYIHTDEGVIGKGAAAATPVITGDFTSGMEEAILGPMRSCLIGQ
NIIQFQQLLQRIQMSCIGNSSAKAAVDIALYDVYCQYQNVPLYALLGGKKEIHTDITVSVDEPVLMAKEAKKHIEKGFQT
LKIKVGKEAHLDLERIEAIRNVVPRNTTLRLDANQGWSPKEAVSIIQEMENRNLNIEFIEQPVHAKDWDGLKYVKDRVQT
PIMADESIFSASDALKLVQGRYADFINIKLMKCGGIREAWRIADIAETAGVKCMVGSMMESSLSVSAVAHLAAAHPNIHY
FDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS

Sequences:

>Translated_353_residues
MNKMKITDVKVNRRRVKLHTPFKTALRTVTEIESIDVYIHTDEGVIGKGAAAATPVITGDFTSGMEEAILGPMRSCLIGQ
NIIQFQQLLQRIQMSCIGNSSAKAAVDIALYDVYCQYQNVPLYALLGGKKEIHTDITVSVDEPVLMAKEAKKHIEKGFQT
LKIKVGKEAHLDLERIEAIRNVVPRNTTLRLDANQGWSPKEAVSIIQEMENRNLNIEFIEQPVHAKDWDGLKYVKDRVQT
PIMADESIFSASDALKLVQGRYADFINIKLMKCGGIREAWRIADIAETAGVKCMVGSMMESSLSVSAVAHLAAAHPNIHY
FDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS
>Mature_353_residues
MNKMKITDVKVNRRRVKLHTPFKTALRTVTEIESIDVYIHTDEGVIGKGAAAATPVITGDFTSGMEEAILGPMRSCLIGQ
NIIQFQQLLQRIQMSCIGNSSAKAAVDIALYDVYCQYQNVPLYALLGGKKEIHTDITVSVDEPVLMAKEAKKHIEKGFQT
LKIKVGKEAHLDLERIEAIRNVVPRNTTLRLDANQGWSPKEAVSIIQEMENRNLNIEFIEQPVHAKDWDGLKYVKDRVQT
PIMADESIFSASDALKLVQGRYADFINIKLMKCGGIREAWRIADIAETAGVKCMVGSMMESSLSVSAVAHLAAAHPNIHY
FDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=324, Percent_Identity=32.4074074074074, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI48994953, Length=307, Percent_Identity=28.3387622149837, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39204; Mature: 39204

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKMKITDVKVNRRRVKLHTPFKTALRTVTEIESIDVYIHTDEGVIGKGAAAATPVITGD
CCCEEEEEEEECCEEEEEECCHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCEEECC
FTSGMEEAILGPMRSCLIGQNIIQFQQLLQRIQMSCIGNSSAKAAVDIALYDVYCQYQNV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCC
PLYALLGGKKEIHTDITVSVDEPVLMAKEAKKHIEKGFQTLKIKVGKEAHLDLERIEAIR
CEEEEECCCEEECCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
NVVPRNTTLRLDANQGWSPKEAVSIIQEMENRNLNIEFIEQPVHAKDWDGLKYVKDRVQT
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCC
PIMADESIFSASDALKLVQGRYADFINIKLMKCGGIREAWRIADIAETAGVKCMVGSMME
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
SSLSVSAVAHLAAAHPNIHYFDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS
HHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEECCCEEEECCCCCCCC
>Mature Secondary Structure
MNKMKITDVKVNRRRVKLHTPFKTALRTVTEIESIDVYIHTDEGVIGKGAAAATPVITGD
CCCEEEEEEEECCEEEEEECCHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCEEECC
FTSGMEEAILGPMRSCLIGQNIIQFQQLLQRIQMSCIGNSSAKAAVDIALYDVYCQYQNV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCC
PLYALLGGKKEIHTDITVSVDEPVLMAKEAKKHIEKGFQTLKIKVGKEAHLDLERIEAIR
CEEEEECCCEEECCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
NVVPRNTTLRLDANQGWSPKEAVSIIQEMENRNLNIEFIEQPVHAKDWDGLKYVKDRVQT
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCC
PIMADESIFSASDALKLVQGRYADFINIKLMKCGGIREAWRIADIAETAGVKCMVGSMME
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
SSLSVSAVAHLAAAHPNIHYFDLDAPLWLMEEPEGMTYSGSKVNLQSTVNSKS
HHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEECCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]