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The map label for this gene is amaA [H]

Identifier: 218232305

GI number: 218232305

Start: 1374302

End: 1375471

Strand: Direct

Name: amaA [H]

Synonym: BCB4264_A1426

Alternate gene names: 218232305

Gene position: 1374302-1375471 (Clockwise)

Preceding gene: 218235363

Following gene: 218233115

Centisome position: 25.36

GC content: 37.35

Gene sequence:

>1170_bases
ATGAATAGAGTGTGGAAGAATCTAATTTCGGAAGAAAATATCGTGAAATGGAGAAGGCATTTCCATAAGTATCCGGAATT
ATCATTTCATGAAAAAGAAACTTCGCAATTTATATATGATACATTATGCTCATTTTCTTCTTTTGAAGTAACGAGACCGA
CGAAGTATAGTGTACTAGCAATTAAAAGAGGTGTGAAGCAAGGGAAAGTAATTGCGATTCGAGCTGATATAGACGCTTTG
CCAATTCAAGAGGAGACAAGTAAATCTTATACATCTGTGAATAAAGGAATGATGCATGCATGTGGGCACGATGCTCACGC
AGCTATTTTATTAAGTACAGCAGAGGTGTTATCAAATATAAAGGAAGATTTTGCAGGTGAGATTCGTCTATTCTTTCAGC
ACGCAGAAGAGATGTATCCCGGTGGGGGACAAGAAATGGTTGAGGCAGGTGTAATGGACGGTGTTGATTATGTAATAGGT
TTACATGTTATGTCAGGACTTGAGAGTGGAAAGATAGGAATTGCATATGGACCGATGATGGCAGCTCCAGACGTATTTAC
AGTTGAGATTCAAGGCAAAGGAGGACATGCGGCGCGACCAGAAGAAACGATAGATCCTATTGCTATCGGAGCACAGATTA
TTACAAATCTACAACATATCGTATCAAGAAATACAAGTGCTTTTATGCAAAGGGTTGTTTCAGTTACACAATTTCATGGG
GGAACGGCTGACAATATTATTCCTAATACAGCAACTTTAATGGGAACTGTTCGTTCATTTAATCAAGCATTGAGGGTGGA
AGCAGAAGGGAAAATCGAGAAAATTGTGAAAGGAATTACAGAAGCACATGGAGGATCGTATACATATACGTATCGATATG
GATATGATCCGGTTATTAATGATGAATATATTACGAAAATAGTAGAAGAAAGTGCACTACATTTGTTTGGGAATGAGCGT
GTTGTGAAGCTTGAACCTTCTATGGGTGGAGAAGATTTTTCAGCATATTTAAGAAAAGCACCTGGTTGTTTCATTAAATT
AGGAACAGGTAATGAAAATATATATACTTGTTATCCACATCATCATCCGAAATTTGACGTGGATGAATCAGCATTAATTT
GTGGAGTGGAGCTATTTTTAGAGACGACTATAAGATTACTAAAATCATAG

Upstream 100 bases:

>100_bases
ACTATAAGAATACGTAAAAATACAGTGTTTGTTTTTATTAATCATTTAAGACGGAGTAAACTTTTAAATTTCTAGTTCTT
TTGAAAAGGAGGAAATTATC

Downstream 100 bases:

>100_bases
AAAAACAAAAAAAGGCAACTGCACTCTATTGCAGTTGCTTTTATTTCTTTCCGTCAGAGAGGGCTGAGAAAGAACTATGC
TCATTCATAGTATATGAATA

Product: aminoacylase

Products: NA

Alternate protein names: L-aminoacylase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNRVWKNLISEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCSFSSFEVTRPTKYSVLAIKRGVKQGKVIAIRADIDAL
PIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNIKEDFAGEIRLFFQHAEEMYPGGGQEMVEAGVMDGVDYVIG
LHVMSGLESGKIGIAYGPMMAAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
GTADNIIPNTATLMGTVRSFNQALRVEAEGKIEKIVKGITEAHGGSYTYTYRYGYDPVINDEYITKIVEESALHLFGNER
VVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNENIYTCYPHHHPKFDVDESALICGVELFLETTIRLLKS

Sequences:

>Translated_389_residues
MNRVWKNLISEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCSFSSFEVTRPTKYSVLAIKRGVKQGKVIAIRADIDAL
PIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNIKEDFAGEIRLFFQHAEEMYPGGGQEMVEAGVMDGVDYVIG
LHVMSGLESGKIGIAYGPMMAAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
GTADNIIPNTATLMGTVRSFNQALRVEAEGKIEKIVKGITEAHGGSYTYTYRYGYDPVINDEYITKIVEESALHLFGNER
VVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNENIYTCYPHHHPKFDVDESALICGVELFLETTIRLLKS
>Mature_389_residues
MNRVWKNLISEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCSFSSFEVTRPTKYSVLAIKRGVKQGKVIAIRADIDAL
PIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNIKEDFAGEIRLFFQHAEEMYPGGGQEMVEAGVMDGVDYVIG
LHVMSGLESGKIGIAYGPMMAAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
GTADNIIPNTATLMGTVRSFNQALRVEAEGKIEKIVKGITEAHGGSYTYTYRYGYDPVINDEYITKIVEESALHLFGNER
VVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNENIYTCYPHHHPKFDVDESALICGVELFLETTIRLLKS

Specific function: Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L- stereospecific [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=423, Percent_Identity=25.531914893617, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.14 [H]

Molecular weight: Translated: 43204; Mature: 43204

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVWKNLISEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCSFSSFEVTRPTKYSVLA
CCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
IKRGVKQGKVIAIRADIDALPIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNI
HHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEDFAGEIRLFFQHAEEMYPGGGQEMVEAGVMDGVDYVIGLHVMSGLESGKIGIAYGPMM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCC
AAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
GTADNIIPNTATLMGTVRSFNQALRVEAEGKIEKIVKGITEAHGGSYTYTYRYGYDPVIN
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
DEYITKIVEESALHLFGNERVVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNENIYTCYPH
HHHHHHHHHHHHHHEECCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEECCC
HHPKFDVDESALICGVELFLETTIRLLKS
CCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRVWKNLISEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCSFSSFEVTRPTKYSVLA
CCHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
IKRGVKQGKVIAIRADIDALPIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNI
HHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEDFAGEIRLFFQHAEEMYPGGGQEMVEAGVMDGVDYVIGLHVMSGLESGKIGIAYGPMM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCC
AAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
GTADNIIPNTATLMGTVRSFNQALRVEAEGKIEKIVKGITEAHGGSYTYTYRYGYDPVIN
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
DEYITKIVEESALHLFGNERVVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNENIYTCYPH
HHHHHHHHHHHHHHEECCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEECCC
HHPKFDVDESALICGVELFLETTIRLLKS
CCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8285691; 9023955 [H]