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The map label for this gene is bkdR [H]

Identifier: 218232263

GI number: 218232263

Start: 849323

End: 849817

Strand: Direct

Name: bkdR [H]

Synonym: BCB4264_A0883

Alternate gene names: 218232263

Gene position: 849323-849817 (Clockwise)

Preceding gene: 218232227

Following gene: 218232868

Centisome position: 15.67

GC content: 36.97

Gene sequence:

>495_bases
ATGGAGTCGTCTGTTATTAAAGTGTTGGATGATTTGGATGTACAAATTTTAGATATATTGCAGAAGGAGTCACAAGTAAG
TAATGCAGAGCTGGCACGTCGCGTTAATTTATCACCGGCTGCGATGCATGCAAGAATAAAAAGGTTAGATGGGGAAGGGT
TCATCGATAAGCAAGTTGCGATTTTAAATCAAGAAAAGCTTGGGTTTGATTTATTATGCTTTATTTTTATGAGTACGAAT
ATTCATCAATTTGACAAGTTGGAAGTGTTGGAGAAAGAATTAGAATCGATGCCGGAAGTGTTAGAATGTCACTGTTTAAC
AGGGGAGTACGATTATTTATTAAAGGTTGCAAATCGTGATCGTAAAGAACTAGAGCAGTTTATTAGAAAGCTGAATAAGC
TTGGTATTACACGTATACAGACGAGTTTGGCACTTCGTGAAATTAAATATTCAACAGTATTGCCGATACGAGATGAGGAA
CCGAGCATCGATTAG

Upstream 100 bases:

>100_bases
ATTTATCTGTGAATGATATTTACAGAATGATATTCAGTTTTGGATAAATTATAAGGTGTTTTTTCGAATAATCATCGGTA
AGAAAAAAGGGGGAATTGTA

Downstream 100 bases:

>100_bases
ATTGCTTGGTTTTTGGTTTGTATAAATTGATTGACGAGGAGAAAAGAGAGAGGTATTATTATATAAAATTATGAATTAAA
ATTCATTTTAAGTTATAAAT

Product: transcriptional regulator, AsnC family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MESSVIKVLDDLDVQILDILQKESQVSNAELARRVNLSPAAMHARIKRLDGEGFIDKQVAILNQEKLGFDLLCFIFMSTN
IHQFDKLEVLEKELESMPEVLECHCLTGEYDYLLKVANRDRKELEQFIRKLNKLGITRIQTSLALREIKYSTVLPIRDEE
PSID

Sequences:

>Translated_164_residues
MESSVIKVLDDLDVQILDILQKESQVSNAELARRVNLSPAAMHARIKRLDGEGFIDKQVAILNQEKLGFDLLCFIFMSTN
IHQFDKLEVLEKELESMPEVLECHCLTGEYDYLLKVANRDRKELEQFIRKLNKLGITRIQTSLALREIKYSTVLPIRDEE
PSID
>Mature_164_residues
MESSVIKVLDDLDVQILDILQKESQVSNAELARRVNLSPAAMHARIKRLDGEGFIDKQVAILNQEKLGFDLLCFIFMSTN
IHQFDKLEVLEKELESMPEVLECHCLTGEYDYLLKVANRDRKELEQFIRKLNKLGITRIQTSLALREIKYSTVLPIRDEE
PSID

Specific function: Positive regulator of the bkd operon for branched-chain keto acid dehydrogenase complex [H]

COG id: COG1522

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH asnC-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081742, Length=151, Percent_Identity=35.7615894039735, Blast_Score=97, Evalue=4e-22,
Organism=Escherichia coli, GI1787116, Length=155, Percent_Identity=36.1290322580645, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790182, Length=134, Percent_Identity=31.3432835820896, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011008
- InterPro:   IPR000485
- InterPro:   IPR019885
- InterPro:   IPR019888
- InterPro:   IPR019887
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01037 AsnC_trans_reg [H]

EC number: NA

Molecular weight: Translated: 18932; Mature: 18932

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00519 HTH_ASNC_1 ; PS50956 HTH_ASNC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSVIKVLDDLDVQILDILQKESQVSNAELARRVNLSPAAMHARIKRLDGEGFIDKQVA
CCCHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHH
ILNQEKLGFDLLCFIFMSTNIHQFDKLEVLEKELESMPEVLECHCLTGEYDYLLKVANRD
HHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
RKELEQFIRKLNKLGITRIQTSLALREIKYSTVLPIRDEEPSID
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MESSVIKVLDDLDVQILDILQKESQVSNAELARRVNLSPAAMHARIKRLDGEGFIDKQVA
CCCHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHH
ILNQEKLGFDLLCFIFMSTNIHQFDKLEVLEKELESMPEVLECHCLTGEYDYLLKVANRD
HHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
RKELEQFIRKLNKLGITRIQTSLALREIKYSTVLPIRDEEPSID
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8320210 [H]