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The map label for this gene is purN [H]

Identifier: 218232251

GI number: 218232251

Start: 318401

End: 318988

Strand: Direct

Name: purN [H]

Synonym: BCB4264_A0343

Alternate gene names: 218232251

Gene position: 318401-318988 (Clockwise)

Preceding gene: 218231041

Following gene: 218231618

Centisome position: 5.88

GC content: 36.39

Gene sequence:

>588_bases
ATGAGTAGATTAGCAGTTTTTGCTTCTGGAAGTGGATCTAACTTTCAATCTCTCGTTAATGCGGTAGAAGAAAAAAGATT
GGATGCAGAAATTAGTTTATTAGTATGTGATAAACCAGAAGCACGAGCTGTTGGACGGGCAAATTATCATCATATTCCGT
GTTTTGCTTTTTCAGCGAAAGCATATGAGTCAAAAGAAGCGTTTGAAAAAGAGATATTAAAGAAGCTAGAAGAATATGAA
ATTGATTATGTTATTTTAGCTGGATATATGCGTTTAATCGGGCCGACGTTACTAGAAGCATACGGCGGGAAGATTATTAA
TATTCATCCATCATTACTACCGAGCTTTCCGGGTAAAGATGCTGTCGGTCAAGCGTTAGAAGCAGGTGTGAAAGTAACTG
GAGTAACAATTCATTATGTAGATGCAGGTATGGATACAGGGCCAATTATTGCACAAGAAGCAGTGGTTGTTTCTGAAGGG
GATACGAGAGAAAGCTTACAAAAGAAAATTCAACAAGTTGAACATAAATTATACGTAAATACAGTGAATCAAATTGTTCA
GTCTGTGAAAGAATCAACTGTTAACTAA

Upstream 100 bases:

>100_bases
AAGATGTTGTTCGTCTTCTTGAAGAGCAAGGAGAAATGGCTCGTATTATTGGACGTACTATACAAGGAGCTGGCGTTACT
TTTAATGGGGGCACAGCACT

Downstream 100 bases:

>100_bases
CATACAACCAGGGGTGAAGGATAAATGAAAAAGCGTGCATTAGTAAGTGTTTCAGATAAAACAGGAGTAGTAGAATTTGT
TAAAGGGTTACTAGAACAAG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAKAYESKEAFEKEILKKLEEYE
IDYVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKDAVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEG
DTRESLQKKIQQVEHKLYVNTVNQIVQSVKESTVN

Sequences:

>Translated_195_residues
MSRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAKAYESKEAFEKEILKKLEEYE
IDYVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKDAVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEG
DTRESLQKKIQQVEHKLYVNTVNQIVQSVKESTVN
>Mature_194_residues
SRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAKAYESKEAFEKEILKKLEEYEI
DYVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKDAVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEGD
TRESLQKKIQQVEHKLYVNTVNQIVQSVKESTVN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=177, Percent_Identity=41.8079096045198, Blast_Score=151, Evalue=3e-37,
Organism=Homo sapiens, GI209869995, Length=177, Percent_Identity=41.8079096045198, Blast_Score=151, Evalue=3e-37,
Organism=Homo sapiens, GI209869993, Length=177, Percent_Identity=41.8079096045198, Blast_Score=151, Evalue=3e-37,
Organism=Escherichia coli, GI1788846, Length=175, Percent_Identity=34.8571428571429, Blast_Score=136, Evalue=7e-34,
Organism=Escherichia coli, GI1787483, Length=172, Percent_Identity=30.8139534883721, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=189, Percent_Identity=34.9206349206349, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=215, Percent_Identity=26.046511627907, Blast_Score=77, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582400, Length=186, Percent_Identity=37.0967741935484, Blast_Score=143, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21311; Mature: 21179

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAK
CCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCH
AYESKEAFEKEILKKLEEYEIDYVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCH
AVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEGDTRESLQKKIQQVEHKLYVN
HHHHHHHCCCEEEEEEEEEEECCCCCCCCEECCEEEEECCCCHHHHHHHHHHHHHHHHHH
TVNQIVQSVKESTVN
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRLAVFASGSGSNFQSLVNAVEEKRLDAEISLLVCDKPEARAVGRANYHHIPCFAFSAK
CCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCH
AYESKEAFEKEILKKLEEYEIDYVILAGYMRLIGPTLLEAYGGKIINIHPSLLPSFPGKD
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCH
AVGQALEAGVKVTGVTIHYVDAGMDTGPIIAQEAVVVSEGDTRESLQKKIQQVEHKLYVN
HHHHHHHCCCEEEEEEEEEEECCCCCCCCEECCEEEEECCCCHHHHHHHHHHHHHHHHHH
TVNQIVQSVKESTVN
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]