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The map label for this gene is malS [H]

Identifier: 218232249

GI number: 218232249

Start: 1716701

End: 1718413

Strand: Direct

Name: malS [H]

Synonym: BCB4264_A1812

Alternate gene names: 218232249

Gene position: 1716701-1718413 (Clockwise)

Preceding gene: 218233183

Following gene: 218233041

Centisome position: 31.68

GC content: 39.58

Gene sequence:

>1713_bases
ATGAGCAAGTTTACAGTAGCTTCTAATGGTGCATTAGAAACAACATTAAGAGGAGCAGAAGTATTATCAACACCACTTTT
AAATAAAGGGGTAGCTTTCACACAAAATGAAAGAGAAGAGTTGGGTTTAAAAGGGTTATTACCGCCAGCGGTTTTAACGT
TAGAAGAACAGGCACGCCGAGCATACGAACAATTTAGTTCTCAGCCGGATGATTTATTAAAGAATGTATACTTAACTGCG
TTACATGATCGAAATGAAGTATTATTTTATCGTATATTAACAGATCATTTACGTGAAATGTTACCGATTGTATATACACC
AACTGTCGGTGTAGCAATTCAAAGATATAGTCATGAATATCGTAAACCACGTGGTGTTTATTTATCAATTAACGATCCAT
CAGGTATCGAAGAAGCATTCACGAATATCGGTGCAACAGCTGAGAACATTGATTTAGTCGTTGTAACAGATGGAGAAGGC
ATATTAGGAATTGGTGACTGGGGCGTTGGTGGTATTAACATCGCAATCGGAAAATTAGCTGTTTACACTGCTGCAGTTGG
AATCGATCCTAGTCGTGTATTACCGGTAATTTTAGATGTAGGTACAAACCGTGAGGAGCTATTAAACAATCCATTTTATA
TTGGAAATCGTCACCCTCGTATAACAGGTGAAGCTTACGATGAATTTATTGATACGTTTGTACAAGCGGTAAACAAACAA
TTCCCGAAAGCACTTTTACATTGGGAAGACTTTAGCTCTCGTAACGCACGAAAAATTTTAGATAAATACCGTCATGACGT
TTGTACGTTTAATGATGATATTCAAGGTACAGGTGCAGTTTCACTTGCGGCAGTATTATCGGCAGTGAAAGCTTCTGGTG
TACCGCTAAGTGAACACCGCGTTGTTGTATTTGGTGCTGGTACGGCTGGAATCGGTATCGCAGATCAAGTTAGAGATGCG
ATGGTCCGCGTAGGCGTATCTGAAGAAGAATCATATAAGCGTTTCTGGTGTATTGATCGTAACGGGTTAGTTACAGATAA
TATGGAAGATCTTCTTGATTTCCAAATTCCGTACGCAAGAAAAGAAGCAGAAGTAAGTGAGTGGAAAGAAAATGATGTGA
TCGGACTTGCTGAAGTTGTGAAACATGTAAAACCGACAATTTTAATTGGTACATCTACTGTTGCAGGTGCATTTAAAGAA
GAGATTATTAAAGAAATGGCTTCTCACGTAGAAAGACCAATCATTTTACCAATGTCGAATCCAACTCCACTTGCTGAAGC
AAAACCAGCAGATTTAATCGAGTGGACAGAAGGAAGAGCGTTAGTTGCAACAGGAAGTCCATTTGAACCAGTTACATATA
ACGGTGTAACGTATGTGATTGGACAATCAAATAATGCACTTATTTTCCCAGGGCTTGGTCTTGGAACAATTGTAGTACGA
GCAAGCGTCATGACGGATGGAATGTTCGCAGCGGCTGCTGAAGCAGTTGCAAGTATGGTAGATACAAGTCAGCCAGGAGC
ACCTATTTTACCAGAGGTCGAAGAATTACGTAATATCTCAGAAATGGTTGCAATTGAAGTAGCGAAAGTTGCAGTTGCAG
AAGGTGTTGCAAGAGTGAACTTAAGTGATAACGATATCAAGATGGCAGTGCAAGAAGCAATGTGGAAGCCAGAGTATCGC
CAAATAAAAGCGGTAGAAAAGGTTAGAATATAG

Upstream 100 bases:

>100_bases
GAGCAACTCTTTTAAAGAAAAATTAAAAGAAGAGGGGGACAATCCCCTCTTTTTTGTTTACAATATAGAAATGCTATATA
TGAATAGAGGTAGGGATGTT

Downstream 100 bases:

>100_bases
AAAAAAAGCCCGTTTATAACAATAAGCGGGCTTTCACTTTTACTACTGAAAAAAAGTATAGGAAGTGTCACCTTTGAATT
GGAATCAATTATGGAAGAAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 3 [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MSKFTVASNGALETTLRGAEVLSTPLLNKGVAFTQNEREELGLKGLLPPAVLTLEEQARRAYEQFSSQPDDLLKNVYLTA
LHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEYRKPRGVYLSINDPSGIEEAFTNIGATAENIDLVVVTDGEG
ILGIGDWGVGGINIAIGKLAVYTAAVGIDPSRVLPVILDVGTNREELLNNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQ
FPKALLHWEDFSSRNARKILDKYRHDVCTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHRVVVFGAGTAGIGIADQVRDA
MVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYARKEAEVSEWKENDVIGLAEVVKHVKPTILIGTSTVAGAFKE
EIIKEMASHVERPIILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVR
ASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARVNLSDNDIKMAVQEAMWKPEYR
QIKAVEKVRI

Sequences:

>Translated_570_residues
MSKFTVASNGALETTLRGAEVLSTPLLNKGVAFTQNEREELGLKGLLPPAVLTLEEQARRAYEQFSSQPDDLLKNVYLTA
LHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEYRKPRGVYLSINDPSGIEEAFTNIGATAENIDLVVVTDGEG
ILGIGDWGVGGINIAIGKLAVYTAAVGIDPSRVLPVILDVGTNREELLNNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQ
FPKALLHWEDFSSRNARKILDKYRHDVCTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHRVVVFGAGTAGIGIADQVRDA
MVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYARKEAEVSEWKENDVIGLAEVVKHVKPTILIGTSTVAGAFKE
EIIKEMASHVERPIILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVR
ASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARVNLSDNDIKMAVQEAMWKPEYR
QIKAVEKVRI
>Mature_569_residues
SKFTVASNGALETTLRGAEVLSTPLLNKGVAFTQNEREELGLKGLLPPAVLTLEEQARRAYEQFSSQPDDLLKNVYLTAL
HDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEYRKPRGVYLSINDPSGIEEAFTNIGATAENIDLVVVTDGEGI
LGIGDWGVGGINIAIGKLAVYTAAVGIDPSRVLPVILDVGTNREELLNNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQF
PKALLHWEDFSSRNARKILDKYRHDVCTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHRVVVFGAGTAGIGIADQVRDAM
VRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYARKEAEVSEWKENDVIGLAEVVKHVKPTILIGTSTVAGAFKEE
IIKEMASHVERPIILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVRA
SVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARVNLSDNDIKMAVQEAMWKPEYRQ
IKAVEKVRI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=546, Percent_Identity=41.9413919413919, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI62420880, Length=546, Percent_Identity=41.9413919413919, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI239049447, Length=546, Percent_Identity=41.9413919413919, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI4505143, Length=554, Percent_Identity=40.7942238267148, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI4505145, Length=547, Percent_Identity=39.4881170018282, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI270265879, Length=458, Percent_Identity=42.3580786026201, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI87081919, Length=548, Percent_Identity=47.2627737226277, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17537199, Length=552, Percent_Identity=43.4782608695652, Blast_Score=439, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322823, Length=578, Percent_Identity=44.636678200692, Blast_Score=502, Evalue=1e-143,
Organism=Drosophila melanogaster, GI281362672, Length=550, Percent_Identity=40.3636363636364, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI21356279, Length=550, Percent_Identity=40.3636363636364, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281362674, Length=550, Percent_Identity=40.3636363636364, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24646386, Length=559, Percent_Identity=38.4615384615385, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24646388, Length=559, Percent_Identity=38.4615384615385, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281363505, Length=563, Percent_Identity=34.1030195381883, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707236, Length=563, Percent_Identity=34.1030195381883, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707242, Length=542, Percent_Identity=35.7933579335793, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707238, Length=542, Percent_Identity=35.7933579335793, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281363503, Length=536, Percent_Identity=34.7014925373134, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI78707232, Length=537, Percent_Identity=34.6368715083799, Blast_Score=354, Evalue=1e-97,
Organism=Drosophila melanogaster, GI78707240, Length=518, Percent_Identity=36.2934362934363, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI19922384, Length=560, Percent_Identity=29.1071428571429, Blast_Score=260, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 62272; Mature: 62141

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFTVASNGALETTLRGAEVLSTPLLNKGVAFTQNEREELGLKGLLPPAVLTLEEQARR
CCCEEECCCCCHHHHHCCHHHHHCCHHCCCCEECCCCHHHCCCCCCCCHHHHHHHHHHHH
AYEQFSSQPDDLLKNVYLTALHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEY
HHHHHCCCHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RKPRGVYLSINDPSGIEEAFTNIGATAENIDLVVVTDGEGILGIGDWGVGGINIAIGKLA
CCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHE
VYTAAVGIDPSRVLPVILDVGTNREELLNNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQ
EHHHEECCCHHHCEEEEEECCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
FPKALLHWEDFSSRNARKILDKYRHDVCTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHR
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
VVVFGAGTAGIGIADQVRDAMVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYAR
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCC
KEAEVSEWKENDVIGLAEVVKHVKPTILIGTSTVAGAFKEEIIKEMASHVERPIILPMSN
CCCHHHHHCCCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCC
PTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVR
CCCCCCCCCCCCEEECCCCEEEECCCCCCCEEECCEEEEEECCCCEEEECCCCHHHHHHH
ASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARVN
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEC
LSDNDIKMAVQEAMWKPEYRQIKAVEKVRI
CCCCHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SKFTVASNGALETTLRGAEVLSTPLLNKGVAFTQNEREELGLKGLLPPAVLTLEEQARR
CCEEECCCCCHHHHHCCHHHHHCCHHCCCCEECCCCHHHCCCCCCCCHHHHHHHHHHHH
AYEQFSSQPDDLLKNVYLTALHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEY
HHHHHCCCHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RKPRGVYLSINDPSGIEEAFTNIGATAENIDLVVVTDGEGILGIGDWGVGGINIAIGKLA
CCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHE
VYTAAVGIDPSRVLPVILDVGTNREELLNNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQ
EHHHEECCCHHHCEEEEEECCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
FPKALLHWEDFSSRNARKILDKYRHDVCTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHR
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
VVVFGAGTAGIGIADQVRDAMVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYAR
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCC
KEAEVSEWKENDVIGLAEVVKHVKPTILIGTSTVAGAFKEEIIKEMASHVERPIILPMSN
CCCHHHHHCCCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCC
PTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVR
CCCCCCCCCCCCEEECCCCEEEECCCCCCCEEECCEEEEEECCCCEEEECCCCHHHHHHH
ASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARVN
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEC
LSDNDIKMAVQEAMWKPEYRQIKAVEKVRI
CCCCHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]