Click here to switch to the map view.

The map label for this gene is opuAB [H]

Identifier: 218232077

GI number: 218232077

Start: 2691076

End: 2691912

Strand: Reverse

Name: opuAB [H]

Synonym: BCB4264_A2795

Alternate gene names: 218232077

Gene position: 2691912-2691076 (Counterclockwise)

Preceding gene: 218234904

Following gene: 218233243

Centisome position: 49.68

GC content: 35.24

Gene sequence:

>837_bases
ATGAATAGTATACCACGTATTCCATTAGGGGAATGGGTCGACTCATTTGTTGCAAGTTTATATGAGCACTTTGAAGGCTT
ATTCCGAGGATTCTCTTATATTATTGGTGGATTCGTTGATTTACTCACTAATTTTTTAACAATAATACCTGCCATATTGA
TGATTATTATCTTGTGTTTCTTCATTTGGTACACAACGAGGAAATTATCTTTAGTCATTTTTACGCTTATTGGATTATTA
TTCATTTTGAATATTAACTACTGGGCACAAACGATGCAAACATTAGCACTCGTACTTACGTCTGTTATTATTTCAATTAT
TGTCGGAATTCCAATTGGTATATTAGCATCTCAAAATGAACGTTTCTCAAAATTTTTAAAACCAACATTAGATTTTATGC
AAACAATGCCTGCATTCGTATACCTTATTCCAGCGATCACATTTTTCGGAGTAGGTGTAGTACCTGGAATTATTGCATCA
GTAATCTTTGCAATGCCACCGACAATTCGTTTTACTGACTTAGGAATTAGACAAGTTCCAGAAGATTTAATTGAAGCAGC
GAATGCATTTGGATCCACTGCATCACAAAAACTATTTAAAGTACAATTACCACTTGCAACCGGAACGATTATGGCTGGTG
TGAACCAAAGCATTATGCTTTCGTTATCGATGGTCGTAACAGCATCACTTGTAGGAGCTCCAGGACTTGGAGTTGATGTA
TATCGCTCTGTAACACAAGTTAATATCGGTATGGGATTTGAAGCTGGATTAGCTATCGTAGTAATCGCAATTATATTAGA
CCGTATTACACAAGGGTTCCATACGAAAAGAAAATAA

Upstream 100 bases:

>100_bases
TTACCAGTAATCGATGAGAAGAAAAGGCTAAGAGGAATTATTAAACGTGAAAGTGTCATTCAAGCTCTTGCAGGAAACAT
TGAGGAAGAGGTGAAAGACG

Downstream 100 bases:

>100_bases
TGACCAAAAAACACACTTTTATAATACAAAGTGTGTTTTTTATTGCATCTAGATTACATATGAACGATAACAGAACCTCC
AGCTGGTGCACAAACTTGGT

Product: glycine betaine/L-proline ABC transporter, permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNSIPRIPLGEWVDSFVASLYEHFEGLFRGFSYIIGGFVDLLTNFLTIIPAILMIIILCFFIWYTTRKLSLVIFTLIGLL
FILNINYWAQTMQTLALVLTSVIISIIVGIPIGILASQNERFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIAS
VIFAMPPTIRFTDLGIRQVPEDLIEAANAFGSTASQKLFKVQLPLATGTIMAGVNQSIMLSLSMVVTASLVGAPGLGVDV
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHTKRK

Sequences:

>Translated_278_residues
MNSIPRIPLGEWVDSFVASLYEHFEGLFRGFSYIIGGFVDLLTNFLTIIPAILMIIILCFFIWYTTRKLSLVIFTLIGLL
FILNINYWAQTMQTLALVLTSVIISIIVGIPIGILASQNERFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIAS
VIFAMPPTIRFTDLGIRQVPEDLIEAANAFGSTASQKLFKVQLPLATGTIMAGVNQSIMLSLSMVVTASLVGAPGLGVDV
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHTKRK
>Mature_278_residues
MNSIPRIPLGEWVDSFVASLYEHFEGLFRGFSYIIGGFVDLLTNFLTIIPAILMIIILCFFIWYTTRKLSLVIFTLIGLL
FILNINYWAQTMQTLALVLTSVIISIIVGIPIGILASQNERFSKFLKPTLDFMQTMPAFVYLIPAITFFGVGVVPGIIAS
VIFAMPPTIRFTDLGIRQVPEDLIEAANAFGSTASQKLFKVQLPLATGTIMAGVNQSIMLSLSMVVTASLVGAPGLGVDV
YRSVTQVNIGMGFEAGLAIVVIAIILDRITQGFHTKRK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=276, Percent_Identity=45.6521739130435, Blast_Score=220, Evalue=6e-59,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=27.2251308900524, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788449, Length=175, Percent_Identity=29.1428571428571, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30432; Mature: 30432

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]