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The map label for this gene is yhcA [H]

Identifier: 218231925

GI number: 218231925

Start: 3121567

End: 3123108

Strand: Reverse

Name: yhcA [H]

Synonym: BCB4264_A3232

Alternate gene names: 218231925

Gene position: 3123108-3121567 (Counterclockwise)

Preceding gene: 218231718

Following gene: 218235749

Centisome position: 57.63

GC content: 37.22

Gene sequence:

>1542_bases
GTGCAAATATGTAAACGATTTAAACGAATCAATGAAATTTATAATGAAGGGGCAATTAAATATATGAGTGAAACATTACA
TAAAAGAAAACCACCATATGTAATGCTTGTAATTCTTTTTATTGGGGCATTTGTTTCATTTCTAAATAACTCGCTTTTAA
ATGTAGCCTTGCCATCAATAATGAAAGATTTAGACATTCAAGATTATTCAACGATCCAGTGGCTTTCTACAGGGTATATG
CTTGTAAGTGGTATATTAATTCCGGCATCAGCATTTTTAATAACGCGTTTCTCGAATAGAAGCCTATTTATTACATCTAT
GTTGATTTTTATTCTTGGTACTGCCTTAGCGGCTGTAGCGCCGAATTTTGGTTTATTACTTACTGGTCGGATGGTTCAAG
CGGCAGGCTCTTCAGTTATGGGGCCTTTGTTAATGAATATTATGCTAGTAAGCTTTCCAAGAGAAAAAAGGGGAACAGCA
ATGGGGATTTTTGGACTAGTTATGATTACAGCTCCAGCAATTGGACCGACACTATCAGGTTATATTGTAGAGTATTATGA
CTGGCGTTTGCTTTTTGAAATGATTTTACCGTTAGCAATCATTAGCTTACTGTTAGGGATTTGGAAATCTGAGAATGTCA
TGAAACAAAACAAAAATGCAAAACTTGATTATCTCTCATTACTATTATCTTCTGTCGGTTTTGGCGGTTTGTTATATGGA
TTCAGTTCTGCAAGCTCCGATGGCTGGACGAATAAAGTTGTCTTAACAGCCTTAATCATAGGCGCTATTGCCTTAATTGC
TTTCATTATCCGTCAATTAAAAATGGATGAGCCGTTATTGGATTTACGTGTTTACAAGTACCCAATGTTTGCACTTGCGT
CTGTAATTGCAATTGTCAACGCGGTGGCCATGTTTTCGGGTATGATTTTAACACCAGCTTATGTACAAAACGTCCGTGGT
ATTTCGCCGCTGAGCTCTGGGTTGATGATGCTTCCAGGTGCGGTAATAATGGGGATTATGTCACCAATTACAGGTAAACT
ATTTGATAAATATGGTCCTCGGATTCTTGGCATAGTAGGGCTTTCCATTACAGCTGTTTCAACTTATATGTTGGCAAGTC
TGCAAATTGACTCTAGTCATACACATATTATTCTTATTTATACACTGCGGATGTTTGGGATGGCGATGGTGATGATGCCA
CTTATGACAAATGGTCTTAATCAATTGCCAACTCGCTTAAATCCGCACGGTACAGCTGTTAATAATACAGCTCAACAAGT
GTCCGGTTCGATTGGTACTGCTATTCTTGTTACGATTATGAATTCTGTAACAAAAACAAAAGTTGAAAGTTTAATGTCTG
ATATAGATCCAACAACCTTCACAGAAACTACGAAGGCAATAGTAACTCAAAAGGCTTTATTATCTGGAATTCAGTATTCC
TTCTATGTAGCGCTTGGCATGAATGTTGTTGCACTACTATTAGTTTTTTTCGTTAAACGTGTAGATACAAGTGCGGAAGC
TGTCGAGAAGTTAAATCGGTAG

Upstream 100 bases:

>100_bases
CAAAAAGATTTTAAAATGTTATGTTGGGCAAGTGGAATTGCTAAAAAGAGAATATCCATTCGAAGAGTTTGGCAAAATAC
TGCAAGGGTAATGTTTTCTT

Downstream 100 bases:

>100_bases
AGAAGAATAAAACGCTAGATAAAGATTCCTATAAGTTTTTTTGCTGCTAAGGCGCCGGTATTTAAAGCTAGAAAAAACTA
AAAGAAGCAGGGAAATAATT

Product: drug resistance transporter, EmrB/QacA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MQICKRFKRINEIYNEGAIKYMSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIQDYSTIQWLSTGYM
LVSGILIPASAFLITRFSNRSLFITSMLIFILGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTA
MGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAIISLLLGIWKSENVMKQNKNAKLDYLSLLLSSVGFGGLLYG
FSSASSDGWTNKVVLTALIIGAIALIAFIIRQLKMDEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRG
ISPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLASLQIDSSHTHIILIYTLRMFGMAMVMMP
LMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMNSVTKTKVESLMSDIDPTTFTETTKAIVTQKALLSGIQYS
FYVALGMNVVALLLVFFVKRVDTSAEAVEKLNR

Sequences:

>Translated_513_residues
MQICKRFKRINEIYNEGAIKYMSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIQDYSTIQWLSTGYM
LVSGILIPASAFLITRFSNRSLFITSMLIFILGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTA
MGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAIISLLLGIWKSENVMKQNKNAKLDYLSLLLSSVGFGGLLYG
FSSASSDGWTNKVVLTALIIGAIALIAFIIRQLKMDEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRG
ISPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLASLQIDSSHTHIILIYTLRMFGMAMVMMP
LMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMNSVTKTKVESLMSDIDPTTFTETTKAIVTQKALLSGIQYS
FYVALGMNVVALLLVFFVKRVDTSAEAVEKLNR
>Mature_513_residues
MQICKRFKRINEIYNEGAIKYMSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLDIQDYSTIQWLSTGYM
LVSGILIPASAFLITRFSNRSLFITSMLIFILGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTA
MGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAIISLLLGIWKSENVMKQNKNAKLDYLSLLLSSVGFGGLLYG
FSSASSDGWTNKVVLTALIIGAIALIAFIIRQLKMDEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRG
ISPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLASLQIDSSHTHIILIYTLRMFGMAMVMMP
LMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMNSVTKTKVESLMSDIDPTTFTETTKAIVTQKALLSGIQYS
FYVALGMNVVALLLVFFVKRVDTSAEAVEKLNR

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=489, Percent_Identity=23.721881390593, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788710, Length=452, Percent_Identity=25.6637168141593, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI87081983, Length=409, Percent_Identity=25.1833740831296, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=25.6218905472637, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=25.4320987654321, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=332, Percent_Identity=24.3975903614458, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=328, Percent_Identity=23.4756097560976, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56053; Mature: 56053

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQICKRFKRINEIYNEGAIKYMSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSI
CHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MKDLDIQDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRSLFITSMLIFILGTALAAVA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHC
PNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSG
CCCCEEEECHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCHHH
YIVEYYDWRLLFEMILPLAIISLLLGIWKSENVMKQNKNAKLDYLSLLLSSVGFGGLLYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
FSSASSDGWTNKVVLTALIIGAIALIAFIIRQLKMDEPLLDLRVYKYPMFALASVIAIVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVG
HHHHHHCHHCCHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
LSITAVSTYMLASLQIDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAV
HHHHHHHHHHHHHEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCC
NNTAQQVSGSIGTAILVTIMNSVTKTKVESLMSDIDPTTFTETTKAIVTQKALLSGIQYS
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHH
FYVALGMNVVALLLVFFVKRVDTSAEAVEKLNR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MQICKRFKRINEIYNEGAIKYMSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSI
CHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
MKDLDIQDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRSLFITSMLIFILGTALAAVA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHC
PNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSG
CCCCEEEECHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCHHH
YIVEYYDWRLLFEMILPLAIISLLLGIWKSENVMKQNKNAKLDYLSLLLSSVGFGGLLYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
FSSASSDGWTNKVVLTALIIGAIALIAFIIRQLKMDEPLLDLRVYKYPMFALASVIAIVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVG
HHHHHHCHHCCHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
LSITAVSTYMLASLQIDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAV
HHHHHHHHHHHHHEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCC
NNTAQQVSGSIGTAILVTIMNSVTKTKVESLMSDIDPTTFTETTKAIVTQKALLSGIQYS
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHH
FYVALGMNVVALLLVFFVKRVDTSAEAVEKLNR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]