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The map label for this gene is colA [H]

Identifier: 218231877

GI number: 218231877

Start: 3769542

End: 3771383

Strand: Reverse

Name: colA [H]

Synonym: BCB4264_A3861

Alternate gene names: 218231877

Gene position: 3771383-3769542 (Counterclockwise)

Preceding gene: 218234139

Following gene: 218231200

Centisome position: 69.6

GC content: 34.69

Gene sequence:

>1842_bases
ATGAAAATATTTCAAAAAATGTGTGCGTCAACTGTAATGATTACAGCACTTTTAGGAACAGGAGGTACTATCAATAGTGT
ACATGCTGATTCTATAGAACAGCAGATTAAATCAGGGATAGAACAAGAAACAGATAAGCAAATCATTGTAAAATTTAAAG
CGGATTTGGATTTGCCATATGAAGATGGTATTGAAAAACAAATACAATCTCAAACGAATGATAAAGTAATAAAAGATCTT
TTGACAGAATATCCAGATGTAACATTTACTCGTTTATTCACATCTGTAAGCCCGGAACAAATACAAAACCTTGAAGTGAA
AGCACCTAATCATGTATCTACAAGTTTATTAAACTACTATATTCTTCAAAATCAAAATAGTGGGTATGAAGAAGAAATAG
TAGATAAGTTAAAGGCATCTTCTTTAATAGAAGATGCATATATGAAGAGAAAAGAGAAAATAATGCCACCTGAAGTGCAA
TCATCAAGTGTAGCTCTTAATCCTAGTAACAATCCTAGATTTAAAAAGCAAGGTTATCTTGAAGCGGCCCCATACGGTAT
TAATGCGCCTTTCGCCTGGGAAATTCAAGGCGGTAATGGAAATGGTATCACTTTTGTTGATATGGAATACGGATGGCTAT
TAAATCATGAGGATTTATTACATCAAAATATTGAATTAATGTCTGGAAGAAATATAGATCAGCATGTCGGTCATGGGACG
TCTGTACTAGGAATTGTATCCTCTGAGGACAATGAAGTCGGGAATATTGGGATTGCGCCAAAAGCGAAAGCAAAGGTTAT
CTCTCAAATAAGAGATAACGGACAGTATAATACGGCTGATGCAATATTAAGCGCTGTAAATCAGTTAGAAGCTGGGGATG
TTTTATTGTTAGAGGCGCAAGCATCTTTCGATGGATATGGTGATAAATATTTACCTGTTGAAGTACAACCAGATATCTTT
GATGCAATTCGTGCTGGTACAGATAAAGGGATTGTAATTATAGAGGCTGGAGCAAATGGGTGGAATGATTTAGACCAGTT
CAAAGATAGAAAAGGTAAGCAAGTCTTAAATCGTAATAGTAAAGATTTTAAAGATTCGGGTGCAATTATGGTAGGCGCTG
GCTCTTCATCTTTCCCTCATGAACGTATGTGGTTTTCGAATTATGGAAGCCGTATTGATGTGTACGGATGGGGAGAAAAT
GTAGATACAACTACAGCTGAACAAAGTAGAAGTGCTGTAAATCTTTATACTTCAAGCTTTAGCGGAACATCTAGTGCTTC
GCCAATTATTGCTGGGGCAGCGACTTTAGTACAAAGCATTGCTAAAGAAAATTTAGGACAACCATATAGACCTAGTGAAC
TAAGAGCAATATTAAGCAACCAGAGCACAGGAACAAAATCTAAAGATCCATATGCAGATAAAATTGGTGTTTTACCAGAT
TTGAAGTCTATACTAGTAAACTTAGGCTATGAACAAAGAAAACCAAACGGTGGGAATGAATTACAAGTAACAGAAAATGA
ACCAAATAATGATCCTAGACAGGCAAATAAAGTTAACTTTCATACACCGGTGAAAGGAACGCTACATAATAGTGATAGAG
TAGATGTATTTACTTTCCAAATTGACTCGCCGGAAAATATTAATATTTCTCTACTAAATGAACAAAATATCGGAATGACA
TGGGTACTTCATCATGAATCAGATTTAAATAACTATGTAGCATATGGTGAAAATGAAGGCAATGTAGTTAAAGGAACTTA
TAATGCAAAGCCAGGTAAATATTATTTATACGTCTATAAATATGAGAATAAAGATGGTTCATACGTATTGAACATAAAAT
AA

Upstream 100 bases:

>100_bases
TCTGATTACTATAATCTATTTAGTAGAAAGGGATACGTTATAGAAATAAAATCATTATAGATTAATATAATAATTAATTA
TTAATAGGAGGATTAATGGA

Downstream 100 bases:

>100_bases
GTAAAAGGAACTTCAGATGAAGTTCCTTTAAATTTTTCTAATTTAGTATATGTGAATGATTTTTTAACTTTACTTCTATG
CAATATTTCATATTGATGAA

Product: serine protease, subtilase family

Products: NA

Alternate protein names: 120 kDa collagenase [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MKIFQKMCASTVMITALLGTGGTINSVHADSIEQQIKSGIEQETDKQIIVKFKADLDLPYEDGIEKQIQSQTNDKVIKDL
LTEYPDVTFTRLFTSVSPEQIQNLEVKAPNHVSTSLLNYYILQNQNSGYEEEIVDKLKASSLIEDAYMKRKEKIMPPEVQ
SSSVALNPSNNPRFKKQGYLEAAPYGINAPFAWEIQGGNGNGITFVDMEYGWLLNHEDLLHQNIELMSGRNIDQHVGHGT
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQASFDGYGDKYLPVEVQPDIF
DAIRAGTDKGIVIIEAGANGWNDLDQFKDRKGKQVLNRNSKDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGEN
VDTTTAEQSRSAVNLYTSSFSGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNQSTGTKSKDPYADKIGVLPD
LKSILVNLGYEQRKPNGGNELQVTENEPNNDPRQANKVNFHTPVKGTLHNSDRVDVFTFQIDSPENINISLLNEQNIGMT
WVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYKYENKDGSYVLNIK

Sequences:

>Translated_613_residues
MKIFQKMCASTVMITALLGTGGTINSVHADSIEQQIKSGIEQETDKQIIVKFKADLDLPYEDGIEKQIQSQTNDKVIKDL
LTEYPDVTFTRLFTSVSPEQIQNLEVKAPNHVSTSLLNYYILQNQNSGYEEEIVDKLKASSLIEDAYMKRKEKIMPPEVQ
SSSVALNPSNNPRFKKQGYLEAAPYGINAPFAWEIQGGNGNGITFVDMEYGWLLNHEDLLHQNIELMSGRNIDQHVGHGT
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQASFDGYGDKYLPVEVQPDIF
DAIRAGTDKGIVIIEAGANGWNDLDQFKDRKGKQVLNRNSKDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGEN
VDTTTAEQSRSAVNLYTSSFSGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNQSTGTKSKDPYADKIGVLPD
LKSILVNLGYEQRKPNGGNELQVTENEPNNDPRQANKVNFHTPVKGTLHNSDRVDVFTFQIDSPENINISLLNEQNIGMT
WVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYKYENKDGSYVLNIK
>Mature_613_residues
MKIFQKMCASTVMITALLGTGGTINSVHADSIEQQIKSGIEQETDKQIIVKFKADLDLPYEDGIEKQIQSQTNDKVIKDL
LTEYPDVTFTRLFTSVSPEQIQNLEVKAPNHVSTSLLNYYILQNQNSGYEEEIVDKLKASSLIEDAYMKRKEKIMPPEVQ
SSSVALNPSNNPRFKKQGYLEAAPYGINAPFAWEIQGGNGNGITFVDMEYGWLLNHEDLLHQNIELMSGRNIDQHVGHGT
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQASFDGYGDKYLPVEVQPDIF
DAIRAGTDKGIVIIEAGANGWNDLDQFKDRKGKQVLNRNSKDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGEN
VDTTTAEQSRSAVNLYTSSFSGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNQSTGTKSKDPYADKIGVLPD
LKSILVNLGYEQRKPNGGNELQVTENEPNNDPRQANKVNFHTPVKGTLHNSDRVDVFTFQIDSPENINISLLNEQNIGMT
WVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYKYENKDGSYVLNIK

Specific function: Unknown

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PKD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013510
- InterPro:   IPR007280
- InterPro:   IPR002169
- InterPro:   IPR013661
- InterPro:   IPR022409
- InterPro:   IPR000601 [H]

Pfam domain/function: PF01752 Peptidase_M9; PF08453 Peptidase_M9_N; PF00801 PKD; PF04151 PPC [H]

EC number: =3.4.24.3 [H]

Molecular weight: Translated: 67749; Mature: 67749

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFQKMCASTVMITALLGTGGTINSVHADSIEQQIKSGIEQETDKQIIVKFKADLDLPY
CHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHCCCCEEEEEEECCCCCCH
EDGIEKQIQSQTNDKVIKDLLTEYPDVTFTRLFTSVSPEQIQNLEVKAPNHVSTSLLNYY
HCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCCEECCCCCHHHHHEEEE
ILQNQNSGYEEEIVDKLKASSLIEDAYMKRKEKIMPPEVQSSSVALNPSNNPRFKKQGYL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCE
EAAPYGINAPFAWEIQGGNGNGITFVDMEYGWLLNHEDLLHQNIELMSGRNIDQHVGHGT
EECCCCCCCCEEEEEECCCCCCEEEEEECCCEEECCHHHHHHCHHHHCCCCHHHHCCCCC
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQ
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEC
ASFDGYGDKYLPVEVQPDIFDAIRAGTDKGIVIIEAGANGWNDLDQFKDRKGKQVLNRNS
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCHHHHCCCC
KDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGENVDTTTAEQSRSAVNLYTSSF
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCEEEEEECCC
SGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNQSTGTKSKDPYADKIGVLPD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCC
LKSILVNLGYEQRKPNGGNELQVTENEPNNDPRQANKVNFHTPVKGTLHNSDRVDVFTFQ
HHHHHHHCCHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCEECCCCCEEEEEEE
IDSPENINISLLNEQNIGMTWVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYK
ECCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEEE
YENKDGSYVLNIK
EECCCCCEEEEEC
>Mature Secondary Structure
MKIFQKMCASTVMITALLGTGGTINSVHADSIEQQIKSGIEQETDKQIIVKFKADLDLPY
CHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHCCCCEEEEEEECCCCCCH
EDGIEKQIQSQTNDKVIKDLLTEYPDVTFTRLFTSVSPEQIQNLEVKAPNHVSTSLLNYY
HCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCCEECCCCCHHHHHEEEE
ILQNQNSGYEEEIVDKLKASSLIEDAYMKRKEKIMPPEVQSSSVALNPSNNPRFKKQGYL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCE
EAAPYGINAPFAWEIQGGNGNGITFVDMEYGWLLNHEDLLHQNIELMSGRNIDQHVGHGT
EECCCCCCCCEEEEEECCCCCCEEEEEECCCEEECCHHHHHHCHHHHCCCCHHHHCCCCC
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQ
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEC
ASFDGYGDKYLPVEVQPDIFDAIRAGTDKGIVIIEAGANGWNDLDQFKDRKGKQVLNRNS
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCHHHHCCCC
KDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGENVDTTTAEQSRSAVNLYTSSF
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCEEEEEECCC
SGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNQSTGTKSKDPYADKIGVLPD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCC
LKSILVNLGYEQRKPNGGNELQVTENEPNNDPRQANKVNFHTPVKGTLHNSDRVDVFTFQ
HHHHHHHCCHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCEECCCCCEEEEEEE
IDSPENINISLLNEQNIGMTWVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYK
ECCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEEE
YENKDGSYVLNIK
EECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8282691; 11792842 [H]