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The map label for this gene is pbpF [H]

Identifier: 218231744

GI number: 218231744

Start: 1443990

End: 1446032

Strand: Direct

Name: pbpF [H]

Synonym: BCB4264_A1510

Alternate gene names: 218231744

Gene position: 1443990-1446032 (Clockwise)

Preceding gene: 218233517

Following gene: 218232472

Centisome position: 26.65

GC content: 35.29

Gene sequence:

>2043_bases
ATGAAATTGAAGAACACTCATTTTAAAAAAATGCTTGAGTGGTTCAATAAGAGGAAGAAACTTCGTAATTCATTAATTGT
ATTGGGAAGTGTATTCGTTACTCTATTTATTGCTGTAAATATAATAATTTCCATTCAAGACATATCTGAATTAAAACAAG
CTGTTCCACAGCCGACTTTAATTTATGATGCAAATAATGAGGTCGCAACTAAATTAGCTTCTTCGAAAACAGAAGGCGTT
AAAAGAAAAGATATTCCTGATATTATGGTTCAGGCAATTGTTGCAGTAGAAGATAAGGAATTTTTTAACCATCATGGTAT
TTATTATAGTGGAATTATAAGTGCGGTTTTTAAAAATATTACAGCAGGAGAAGTTGTAGCTGGGGGAAGTACAATTACAC
AGCAGCTTGCGAAGAATGTATTTCTGACACAAGATCGTACATTTTCTCGTAAAATAAAGGAATACTTTTTAACAAAAAAA
ATAGAACGTACTTATACAAAAGATGAAATTATTGAAATGTATATGAATCAAATTTATTTTGGGGAAGGTGCTTGGGGGAT
AAAGAGGGCAGCTAAATCTTATTTTGATAAAGAGGTAAAAGATTTATCAATTTCAGAAGCTGCAACGATTGCGGGATTAA
TTAAAGCGCCGTCTGCGTATTCGCCTTATAAAAACTTTAATAAATCAATTGAACGACGTAATGTCGTATTAAGTTTAATG
AAAGAGCAAGGGTATATTTCTGACGAACAGTATAATAAAGAAAAAGAGTCAGGCCTTGTATTAAAACGTGGGGTTGATGA
TAAATACAAAGGTAAATACTCCCAATATGTAGACTATATAGTTAGGGAAGCGATGGATAAGTATGAATTAACACAAAATG
AAATTTTAGCAGGTGGTTATCGTATTTATACAGAGCTTGACCCGAAAAAGCAACAAGCTGTAGAAGATGTTGTGAATAAT
GATAGTTATTTCAAAGATAGTGGCTCAGATCAACTTATGCAAACGGGTGTAGTCCTTATAAATCCCAAAACAGGCGGTGT
TCCAGCTTTAGTTGGTGGTAGAGGACCATATCAGTTTTTACAATTTAATCATGCTACGCAATTAAAAAGACAACCAGGTT
CAACGTTAAAGCCTCTAGCTGTATATGTACCCGCTTTAGAACAAGGGTATGAAGTATACGATATATTAAAGGATGAACCA
TTTCATATTAAAGAATATGAACCGAAAAATAGTGATCAGGCTTTTCATGGTAATGTGACAATGTATGAAGCGGTAGCAAA
GTCGTATAATGTTTCCGCTGTGTGGCTATTAGAACAAATAGGATTAGATAAAGGATTGAAATCTTTAGAGCGATTTGGTA
TTCCATTAGTACCAGAAGACCGTACGTATCCAATTGCTTTAGGTGGGATGCATGTAGGGACATCACCATTTGTAATGGCC
CAAGCATATAGTACATTTGCTAATGATGGGGTTCAAGTAGAGGCTCATGCAATTAGAGAAGTTCAAAATGCTGAAGGAGA
AATAATAGGGAAATGGTATAAGAAAGAGACACGAGTGACGAGTGAGAAAATTGCTCAAAAGATGACGTATTTATTAAAAG
GTGTTGTAGAAAAGGGAACAGGTGGAAAAGCGAAAGTTACTAATGTTGATACAGCAGGAAAGACAGGGACTACTCAACTT
GTAAATGGTCCGAGCGTAGGGGCAAAGGACTCGTGGTTTGTAGGATACACACCAGATTTAGTAGGTGCGATTTGGGTAGG
ATATGACAAAACAGATAGCGAGCATTATGTTCCAGGTGGTAGTCAAATTACAACGACGATGTTCCGGGATATCATGAAAA
AAGCAAATGCAAATCCAGCTCAAAAAGCATTCCAGTTATCGCTCATATCTGAGGCTGATTATAAAAAACAATTGACTACA
ATAGAAGAGGAAAAACGAAGAAAAGAAGAAGAGAAGAGAAGGAAAGAAGAGGAACAACAACGAAAACAAGAGCAACAAGA
GTGGCTTGATAAAGTGAAAGAATGGATTCCTTCATTTTGGTAA

Upstream 100 bases:

>100_bases
TTTACACTATAGGAATGACTACGTTATTATAATAAATTTAAAAGGCGGTATATTCTTCCTTTTAAAAAGAAGAGTGTAAA
TAAATGAAGGAAGTGAACGA

Downstream 100 bases:

>100_bases
AAGAAAGAGGCGAATAAAAAAATTCGCCTCTTTTTTTGTATGAAAACAAAATAAAATTTTAATCGGTAGAGATTTTTGTC
TATCTTAATTAGCTATTAAT

Product: penicillin-binding protein

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MKLKNTHFKKMLEWFNKRKKLRNSLIVLGSVFVTLFIAVNIIISIQDISELKQAVPQPTLIYDANNEVATKLASSKTEGV
KRKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNITAGEVVAGGSTITQQLAKNVFLTQDRTFSRKIKEYFLTKK
IERTYTKDEIIEMYMNQIYFGEGAWGIKRAAKSYFDKEVKDLSISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKKQQAVEDVVNN
DSYFKDSGSDQLMQTGVVLINPKTGGVPALVGGRGPYQFLQFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEP
FHIKEYEPKNSDQAFHGNVTMYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
QAYSTFANDGVQVEAHAIREVQNAEGEIIGKWYKKETRVTSEKIAQKMTYLLKGVVEKGTGGKAKVTNVDTAGKTGTTQL
VNGPSVGAKDSWFVGYTPDLVGAIWVGYDKTDSEHYVPGGSQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTT
IEEEKRRKEEEKRRKEEEQQRKQEQQEWLDKVKEWIPSFW

Sequences:

>Translated_680_residues
MKLKNTHFKKMLEWFNKRKKLRNSLIVLGSVFVTLFIAVNIIISIQDISELKQAVPQPTLIYDANNEVATKLASSKTEGV
KRKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNITAGEVVAGGSTITQQLAKNVFLTQDRTFSRKIKEYFLTKK
IERTYTKDEIIEMYMNQIYFGEGAWGIKRAAKSYFDKEVKDLSISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKKQQAVEDVVNN
DSYFKDSGSDQLMQTGVVLINPKTGGVPALVGGRGPYQFLQFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEP
FHIKEYEPKNSDQAFHGNVTMYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
QAYSTFANDGVQVEAHAIREVQNAEGEIIGKWYKKETRVTSEKIAQKMTYLLKGVVEKGTGGKAKVTNVDTAGKTGTTQL
VNGPSVGAKDSWFVGYTPDLVGAIWVGYDKTDSEHYVPGGSQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTT
IEEEKRRKEEEKRRKEEEQQRKQEQQEWLDKVKEWIPSFW
>Mature_680_residues
MKLKNTHFKKMLEWFNKRKKLRNSLIVLGSVFVTLFIAVNIIISIQDISELKQAVPQPTLIYDANNEVATKLASSKTEGV
KRKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNITAGEVVAGGSTITQQLAKNVFLTQDRTFSRKIKEYFLTKK
IERTYTKDEIIEMYMNQIYFGEGAWGIKRAAKSYFDKEVKDLSISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKKQQAVEDVVNN
DSYFKDSGSDQLMQTGVVLINPKTGGVPALVGGRGPYQFLQFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEP
FHIKEYEPKNSDQAFHGNVTMYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
QAYSTFANDGVQVEAHAIREVQNAEGEIIGKWYKKETRVTSEKIAQKMTYLLKGVVEKGTGGKAKVTNVDTAGKTGTTQL
VNGPSVGAKDSWFVGYTPDLVGAIWVGYDKTDSEHYVPGGSQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTT
IEEEKRRKEEEKRRKEEEQQRKQEQQEWLDKVKEWIPSFW

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=531, Percent_Identity=32.391713747646, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI87082258, Length=283, Percent_Identity=39.2226148409894, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788867, Length=519, Percent_Identity=27.7456647398844, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789601, Length=143, Percent_Identity=36.3636363636364, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 76986; Mature: 76986

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKNTHFKKMLEWFNKRKKLRNSLIVLGSVFVTLFIAVNIIISIQDISELKQAVPQPTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IYDANNEVATKLASSKTEGVKRKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNI
EEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
TAGEVVAGGSTITQQLAKNVFLTQDRTFSRKIKEYFLTKKIERTYTKDEIIEMYMNQIYF
CCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEE
GEGAWGIKRAAKSYFDKEVKDLSISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGY
HHCCCCCCHHHCCHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHEEECCE
RIYTELDPKKQQAVEDVVNNDSYFKDSGSDQLMQTGVVLINPKTGGVPALVGGRGPYQFL
EEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCEEEECCCCCCCCEEECCCCCHHHH
QFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEPFHIKEYEPKNSDQAFHGNVT
CCCCHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCH
MYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
HHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHH
QAYSTFANDGVQVEAHAIREVQNAEGEIIGKWYKKETRVTSEKIAQKMTYLLKGVVEKGT
HHHHHHCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGKAKVTNVDTAGKTGTTQLVNGPSVGAKDSWFVGYTPDLVGAIWVGYDKTDSEHYVPGG
CCCEEEEECCCCCCCCCCEEECCCCCCCCCCEEECCCHHHHHHEEECCCCCCCCCCCCCC
SQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTTIEEEKRRKEEEKRRKEEEQQ
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKQEQQEWLDKVKEWIPSFW
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLKNTHFKKMLEWFNKRKKLRNSLIVLGSVFVTLFIAVNIIISIQDISELKQAVPQPTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IYDANNEVATKLASSKTEGVKRKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNI
EEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
TAGEVVAGGSTITQQLAKNVFLTQDRTFSRKIKEYFLTKKIERTYTKDEIIEMYMNQIYF
CCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEE
GEGAWGIKRAAKSYFDKEVKDLSISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGY
HHCCCCCCHHHCCHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHEEECCE
RIYTELDPKKQQAVEDVVNNDSYFKDSGSDQLMQTGVVLINPKTGGVPALVGGRGPYQFL
EEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCEEEECCCCCCCCEEECCCCCHHHH
QFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEPFHIKEYEPKNSDQAFHGNVT
CCCCHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCEECCCH
MYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
HHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHH
QAYSTFANDGVQVEAHAIREVQNAEGEIIGKWYKKETRVTSEKIAQKMTYLLKGVVEKGT
HHHHHHCCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GGKAKVTNVDTAGKTGTTQLVNGPSVGAKDSWFVGYTPDLVGAIWVGYDKTDSEHYVPGG
CCCEEEEECCCCCCCCCCEEECCCCCCCCCCEEECCCHHHHHHEEECCCCCCCCCCCCCC
SQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTTIEEEKRRKEEEKRRKEEEQQ
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKQEQQEWLDKVKEWIPSFW
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]