| Definition | Bacillus cereus B4264, complete genome. |
|---|---|
| Accession | NC_011725 |
| Length | 5,419,036 |
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The map label for this gene is pcrA [H]
Identifier: 218231540
GI number: 218231540
Start: 324772
End: 327033
Strand: Direct
Name: pcrA [H]
Synonym: BCB4264_A0351
Alternate gene names: 218231540
Gene position: 324772-327033 (Clockwise)
Preceding gene: 218232295
Following gene: 218230787
Centisome position: 5.99
GC content: 37.75
Gene sequence:
>2262_bases GTGCAGGCGTATATGAGTATGACAGATAAGTTATTAAACGGTTTAAATCCACAGCAACAAAAAGCAGTACAAACAACGAA TGGACCACTTCTATTAATGGCAGGTGCAGGTAGTGGTAAGACACGTGTGTTAACACATCGTATTGCGTATTTACTTGGTG AAAAAGGTGTAGCACCATGGAATGTACTAGCTATTACCTTCACAAATAAAGCAGCTCGTGAAATGCGTGAGCGTATTGAT ACACTTGTCGGACCAGAAGCAGAAGATATTTGGATTTCGACGTTTCACTCTATGTGTGTACGTATTTTACGACGCGATAT CGATCGTATTGGTATTAATCGTAACTTTACAATCTTAGATTCAGGTGATCAGCTAACTGTAGTCAAAAAAATTATGAAAG AGCGCAATATTGATCCGAAGAAATTTGAGCCGCGCTCTATTTTAGCGGGTATTAGTAATGCGAAAAATGAACTGTTATCT GCGGATAAATATGCAAAAAAAATTACAATGGCTGATCCATATGAAAAATTAACGAGCGATGTATACACAGAGTATCAAAA ACGTCTTTTGAAAAATAATTCATTAGACTTTGATGATTTAATTATGACCACGATTCAGTTATTTGAACGTGTTCCAGAAG TATTAGAGTTTTATCAGCGTAAGTTCCAATATATTCACGTTGATGAGTATCAAGATACGAACAAAGCGCAATATCTTCTT GTTAAACATTTGGCAGCGCGTTTTAAAAATCTTTGCGTTGTAGGTGACTCTGACCAGTCTATTTATCGCTGGCGTGGTGC AGATATTTCTAACATTTTGTCGTTCGAAAAAGACTATGAGAATGCACAAGTAATTTTGTTAGAACAAAACTATCGTTCGT CACAAAATATTTTAAATGCAGCGAATGCTGTTATTGAAAAAAATACGAATCGTAAGCCGAAGAAATTATGGACAGACAAT CAAGTTGGAAGTAAAATCTCGTATTATCGTGCTGCAACTGAAAAGGACGAAGCGTATTTTGTTGCGAAAAAAATTCGTGA CGATATTCAAATGGGGAAACGAAAATATACTGATTTTGCAGTGTTATATCGTACGAATGCTCAGTCTCGTATGGTCGAGG AGATTTTCCTTAAATCTAACATTCCGTACAAAATTGTCGGGGGTACGAAGTTCTACGATCGTAAAGAGATTAAAGATATT TTGGCGTACTTACGTTTAATCGGTAATCCAGATGATGAAATTAGTTTTGCACGTATTATTAACATGCCAAAGCGTGGTAT TGGTGCGACTTCTATCGATAAAATTATTAATTACGGTGTGCAAAACGGAATTTCATTAACTGCTGTATTTGATGAAATTG AGCATGTTGGAGTAAGTGCAAAAATTACAAAGGCAGTAAAAGAATTTGCGAATTTATTAAATAACTGGGTAAATATGCAA GAGTATTTATCTGTTACAGAATTAGTAGAAGAAGTTATTGAAAAAACAGGCTATCGCGATATGTTGAAAAATGAACGTTC TTTAGAAGCGGAAGGTCGTTTAGAAAACTTAGACGAGTTCTTATCTGTTACGCAAACATTTGAATCTCAAAGCGAAGATA AGAGCCTTGTTGCATTCTTAACAGATTTAGCACTTGTTGCTGATATTGATCGTGTAGATGAAGACCCAACTGCTGGTGAA GAAGTTATTTTAATGACGATGCACTCAGCGAAAGGATTAGAGTTCCCGGTTGTCTTTATTGTTGGCTTAGAGGAAGGGAT ATTCCCTCATACTCGTTCTCTTATGGAAGAGGATGAAATGCAAGAAGAACGTCGTCTTGCTTATGTAGGTATTACTCGTG CAGAAGAAGAGCTATATTTATCTAACGCGCAAATGCGTACATTATTTGGTAGAACAAGTATGAACGCTGCGTCTCGATTC ATTACAGAAATCCCGACAGAACTAGTGGAATCATTAAATGAAACAGCGCCAAAGCGTGAAACATCGTTTGGTGCAAAAGG CAGAACGGCAAGTAGCGGCAAAACGACAATCACAACGACAACACGCTCTCGTTCAGCTTTCGCGCGCCCTGCAGCTAAGA CGACAGGCGGCGAACAAATTGGCTGGGCAGTAGGAGATAAAGCTTCCCACCAAAAATGGGGAGTCGGTACAGTTGTAAGT GTAAAAGGTGAAGGTGATGCAAAAGAATTAGATATTGCATTCCCAAGCCCAATTGGTGTTAAACGTTTGTTAGCAAAATT TGCACCTGTGACGAAACAATAG
Upstream 100 bases:
>100_bases AGAAATGGCACAATATGCTGATACAGTAGTTGTTGGAAATATTATTTATGATGATATAAAATCGGCGTTAAAAACAGTTA AAGCAGTAAAAGGAGAGTAG
Downstream 100 bases:
>100_bases AAAGGAATGAGGATATGTCAAAAGAGATAGCAAAAAAACGTATTGAAGAACTGCGTGATTTATTAAATACATTTAACTAT CAATATCACGTATTAGACAA
Product: ATP-dependent DNA helicase PcrA
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 753; Mature: 753
Protein sequence:
>753_residues MQAYMSMTDKLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLAITFTNKAAREMRERID TLVGPEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDSGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLS ADKYAKKITMADPYEKLTSDVYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAVIEKNTNRKPKKLWTDN QVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDI LAYLRLIGNPDDEISFARIINMPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKITKAVKEFANLLNNWVNMQ EYLSVTELVEEVIEKTGYRDMLKNERSLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGE EVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRF ITEIPTELVESLNETAPKRETSFGAKGRTASSGKTTITTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVS VKGEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ
Sequences:
>Translated_753_residues MQAYMSMTDKLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLAITFTNKAAREMRERID TLVGPEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDSGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLS ADKYAKKITMADPYEKLTSDVYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAVIEKNTNRKPKKLWTDN QVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDI LAYLRLIGNPDDEISFARIINMPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKITKAVKEFANLLNNWVNMQ EYLSVTELVEEVIEKTGYRDMLKNERSLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGE EVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRF ITEIPTELVESLNETAPKRETSFGAKGRTASSGKTTITTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVS VKGEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ >Mature_753_residues MQAYMSMTDKLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPWNVLAITFTNKAAREMRERID TLVGPEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILDSGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLS ADKYAKKITMADPYEKLTSDVYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNAANAVIEKNTNRKPKKLWTDN QVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFAVLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDI LAYLRLIGNPDDEISFARIINMPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKITKAVKEFANLLNNWVNMQ EYLSVTELVEEVIEKTGYRDMLKNERSLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFLTDLALVADIDRVDEDPTAGE EVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEMQEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRF ITEIPTELVESLNETAPKRETSFGAKGRTASSGKTTITTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVS VKGEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ
Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]
COG id: COG0210
COG function: function code L; Superfamily I DNA and RNA helicases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]
Homologues:
Organism=Escherichia coli, GI2367296, Length=747, Percent_Identity=39.2235609103079, Blast_Score=531, Evalue=1e-152, Organism=Escherichia coli, GI48994965, Length=643, Percent_Identity=39.6578538102644, Blast_Score=425, Evalue=1e-120, Organism=Escherichia coli, GI1787196, Length=347, Percent_Identity=27.9538904899135, Blast_Score=103, Evalue=6e-23, Organism=Saccharomyces cerevisiae, GI6322369, Length=774, Percent_Identity=29.4573643410853, Blast_Score=244, Evalue=3e-65, Organism=Saccharomyces cerevisiae, GI6324477, Length=691, Percent_Identity=23.7337192474674, Blast_Score=109, Evalue=1e-24,
Paralogues:
None
Copy number: 3000 [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005751 - InterPro: IPR013986 - InterPro: IPR014017 - InterPro: IPR000212 - InterPro: IPR014016 [H]
Pfam domain/function: PF00580 UvrD-helicase [H]
EC number: =3.6.4.12 [H]
Molecular weight: Translated: 85183; Mature: 85183
Theoretical pI: Translated: 7.27; Mature: 7.27
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQAYMSMTDKLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPW CCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCE NVLAITFTNKAAREMRERIDTLVGPEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILD EEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE SGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLSADKYAKKITMADPYEKLTSD CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH VYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHH VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNA HHHHHHHHHCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH ANAVIEKNTNRKPKKLWTDNQVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFA HHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEE VLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDILAYLRLIGNPDDEISFARII EEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH NMPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKITKAVKEFANLLNNWVNMQ CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHH EYLSVTELVEEVIEKTGYRDMLKNERSLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFL HHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH TDLALVADIDRVDEDPTAGEEVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEM HHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH QEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRFITEIPTELVESLNETAPKRE HHHHCEEEEEEECCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC TSFGAKGRTASSGKTTITTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVS CCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCEECEECCCCCCCCCCCCCEEEE VKGEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ EECCCCCCEEEEECCCCCCHHHHHHHHCCCCCC >Mature Secondary Structure MQAYMSMTDKLLNGLNPQQQKAVQTTNGPLLLMAGAGSGKTRVLTHRIAYLLGEKGVAPW CCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCE NVLAITFTNKAAREMRERIDTLVGPEAEDIWISTFHSMCVRILRRDIDRIGINRNFTILD EEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE SGDQLTVVKKIMKERNIDPKKFEPRSILAGISNAKNELLSADKYAKKITMADPYEKLTSD CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH VYTEYQKRLLKNNSLDFDDLIMTTIQLFERVPEVLEFYQRKFQYIHVDEYQDTNKAQYLL HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHH VKHLAARFKNLCVVGDSDQSIYRWRGADISNILSFEKDYENAQVILLEQNYRSSQNILNA HHHHHHHHHCEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH ANAVIEKNTNRKPKKLWTDNQVGSKISYYRAATEKDEAYFVAKKIRDDIQMGKRKYTDFA HHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEE VLYRTNAQSRMVEEIFLKSNIPYKIVGGTKFYDRKEIKDILAYLRLIGNPDDEISFARII EEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH NMPKRGIGATSIDKIINYGVQNGISLTAVFDEIEHVGVSAKITKAVKEFANLLNNWVNMQ CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHH EYLSVTELVEEVIEKTGYRDMLKNERSLEAEGRLENLDEFLSVTQTFESQSEDKSLVAFL HHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHH TDLALVADIDRVDEDPTAGEEVILMTMHSAKGLEFPVVFIVGLEEGIFPHTRSLMEEDEM HHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH QEERRLAYVGITRAEEELYLSNAQMRTLFGRTSMNAASRFITEIPTELVESLNETAPKRE HHHHCEEEEEEECCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC TSFGAKGRTASSGKTTITTTTRSRSAFARPAAKTTGGEQIGWAVGDKASHQKWGVGTVVS CCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCEECEECCCCCCCCCCCCCEEEE VKGEGDAKELDIAFPSPIGVKRLLAKFAPVTKQ EECCCCCCEEEEECCCCCCHHHHHHHHCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9701819; 9384377 [H]