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The map label for this gene is sigW [H]

Identifier: 218231535

GI number: 218231535

Start: 3619542

End: 3620075

Strand: Reverse

Name: sigW [H]

Synonym: BCB4264_A3700

Alternate gene names: 218231535

Gene position: 3620075-3619542 (Counterclockwise)

Preceding gene: 218230759

Following gene: 218232287

Centisome position: 66.8

GC content: 31.46

Gene sequence:

>534_bases
GTGAAAGTAATCCCTTTATATAAAATGATTGTAAAAGAAGAAACGGATGTTTCTTTAGCGAAAAAAGGTGATCATGAAGC
CTTTATAGCGCTTATACATACAGAAAAAGCGAAGATGTATCGAATAGCAAAGGCGATGTTACGTGATGAGACAAATATAG
AGGATGCGATACAGACAACAATTTTAAAAGCCTATGAAAATATAAAAAAATTAAAAAAAGAAGAATTTTTTCAAACTTGG
TTAATTCGAATACTAATAAATGAGTGTAACAATATTATTAGAGCGTATAAAAATGTAATTGTGACAGAAGAGAATGATTA
CATTATGAGTGCGTGTGATCAGTATGAAGATATAGATTTGTGTAATGCGATTCAATCGTTACATGAAGAATTAAGAGCTG
TTACGGTGCTTTATTACTATGAAGACATGAATCAAGAGAGTATAGCAAAGCTGTTAGAAATACCGAAAGGAACAGTGAAA
TCAAGATTATCACGTGCGAGGGAACAACTACAAAAACGATTAAAGATGGAATAG

Upstream 100 bases:

>100_bases
AAATATTCAAAAAAGTGAACCTTTTTCTAGGCTCATACGTCTAATATATGTAAAGTTAAATTGGAAGTGATGAAGTGAGG
ATGAAACAGGAGGAGCAATT

Downstream 100 bases:

>100_bases
GGGGTGTAAAGGATGGATAATGAAAAATTATTTGATGAGAAATTAAAAAAGAGAATAAAAGATGAAAATGTTATAGTACC
TCCAGAGTTAAATGAAAAGA

Product: RNA polymerase sigma-70 factor, ECF subfamily

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MKVIPLYKMIVKEETDVSLAKKGDHEAFIALIHTEKAKMYRIAKAMLRDETNIEDAIQTTILKAYENIKKLKKEEFFQTW
LIRILINECNNIIRAYKNVIVTEENDYIMSACDQYEDIDLCNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEIPKGTVK
SRLSRAREQLQKRLKME

Sequences:

>Translated_177_residues
MKVIPLYKMIVKEETDVSLAKKGDHEAFIALIHTEKAKMYRIAKAMLRDETNIEDAIQTTILKAYENIKKLKKEEFFQTW
LIRILINECNNIIRAYKNVIVTEENDYIMSACDQYEDIDLCNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEIPKGTVK
SRLSRAREQLQKRLKME
>Mature_177_residues
MKVIPLYKMIVKEETDVSLAKKGDHEAFIALIHTEKAKMYRIAKAMLRDETNIEDAIQTTILKAYENIKKLKKEEFFQTW
LIRILINECNNIIRAYKNVIVTEENDYIMSACDQYEDIDLCNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEIPKGTVK
SRLSRAREQLQKRLKME

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=173, Percent_Identity=26.0115606936416, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 20853; Mature: 20853

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIPLYKMIVKEETDVSLAKKGDHEAFIALIHTEKAKMYRIAKAMLRDETNIEDAIQTT
CCCHHHHHHHHHCCCCHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILKAYENIKKLKKEEFFQTWLIRILINECNNIIRAYKNVIVTEENDYIMSACDQYEDIDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCHHH
CNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEIPKGTVKSRLSRAREQLQKRLKME
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVIPLYKMIVKEETDVSLAKKGDHEAFIALIHTEKAKMYRIAKAMLRDETNIEDAIQTT
CCCHHHHHHHHHCCCCHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILKAYENIKKLKKEEFFQTWLIRILINECNNIIRAYKNVIVTEENDYIMSACDQYEDIDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCHHH
CNAIQSLHEELRAVTVLYYYEDMNQESIAKLLEIPKGTVKSRLSRAREQLQKRLKME
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]