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The map label for this gene is amt [H]

Identifier: 218231483

GI number: 218231483

Start: 1177963

End: 1179195

Strand: Reverse

Name: amt [H]

Synonym: BCB4264_A1210

Alternate gene names: 218231483

Gene position: 1179195-1177963 (Counterclockwise)

Preceding gene: 218234684

Following gene: 218235391

Centisome position: 21.76

GC content: 43.55

Gene sequence:

>1233_bases
ATGAATATGGGAGATACAGTTTTTATGTTTGTGACGACAGTAATGGTCATGTTGATGACACCAGGATTGGCACTTTTTTA
TGGAGGTATGGTCCGCAGCAAAAATGTACTCAGTACAACGATGCATAGTTATAGTGCAATGGCTATCGTTTCGATTCAAT
GGATTGTTATCGGCTATTCTTTATCATTCGGACCAGATTGGCACGGGCTTATCGGTACACTTGATTGGTTCGGTCTAAAC
GGAGTTACCTACGCACCAAATCCAGACTACTCATCTACTATTCCTCACAATTTATTCATGATGTTTCAACTCATGTTCGC
CATTTTAACTCCAGCTTTAATTTCAGGTGCATTTGCTGAAAGAATGCGATTTTCTGCCTTTCTTATTTTTATCCTTCTAT
GGACAACGATTGTTTACAATCCTGTCGCTCATTGGGTATGGGGCGTCGGCGGGTGGCTAAGGGAACTTGGTGCACTAGAC
TTCGCTGGCGGAAACGTCGTTCACATCACTTCCGGAGTTGCTGGTCTTGTATTAGCTATTTTCCTTGGAAAACGAAAAAA
CATAAATGGTTCTTCTCCTCACCATTTGCCATTTACAATGTTAGGCGCAGGATTATTATGGTTTGGCTGGTTCGGTTTTA
ACGTCGGAAGCGCATTATCTTTAAATGATGTAGCTTTAACTGCATTTATTAATACAAATATAGCTGCTGCTGCCTCAGCA
TTAACTTGGATGCTTTCAGAGTGGTTCTTCCAATCAAAACCGACTGCGATGGGAGCTGCTTGTGGCGCCGTTTCCGGTCT
AGTCGCTATTACACCAGCTTGCGGGTTCGTTACACCTTTCTCAGCTCTTCTTATCGGAGCAATTGGCGGCGTATTATGCT
TCGGAGCGGTCTTTTTCTTAAAAACAAAATTCGGATACGACGATACACTCGATGCATTCGGATGTCACGGCATCGGTGGA
ACTTGGGGCGGTATTGCGACAGGATTATTTGCAACTACTGCCGTAAACGGTGATGGCGCTAACGGATTATTTTACGGAAA
CGCCTCGCTGCTGTTGAAACAACTTGTAGCGATCGGCGCAACATATGCGTTCACAATTATAATGACGTATGCCATTATTA
AAGCAATCAACTTCTTCCTCCCTGTCCGGGTAGATGAGCACGAGGAACATATGGGACTTGATATTTCGATGCATGGGGAG
AAAGCTTATGAGTATACTGAGCGAGTGAACTAA

Upstream 100 bases:

>100_bases
AATCTTCTGAATTTTTAGTTGATTTTCGGTTTAATTTCGAATATGATATGAAACATAACGAAACGATGTTATTTTTTCTA
ACATTATAGGAGGAGAGCTC

Downstream 100 bases:

>100_bases
AAAAGAGATGCAATCTATCGATGATAGATTGCATCTCTTTTTTGCGTACCAGGCAACATACCCCACCTAGTATAACTGGA
AATATATTAACGGTTTTTTG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNMGDTVFMFVTTVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTAVNGDGANGLFYGNASLLLKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN

Sequences:

>Translated_410_residues
MNMGDTVFMFVTTVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTAVNGDGANGLFYGNASLLLKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN
>Mature_410_residues
MNMGDTVFMFVTTVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTAVNGDGANGLFYGNASLLLKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=407, Percent_Identity=42.997542997543, Blast_Score=306, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=28.7383177570093, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32564042, Length=425, Percent_Identity=28.4705882352941, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17550182, Length=435, Percent_Identity=26.8965517241379, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=27.6887871853547, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324187, Length=423, Percent_Identity=39.9527186761229, Blast_Score=256, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6325396, Length=412, Percent_Identity=36.6504854368932, Blast_Score=233, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=36.6028708133971, Blast_Score=225, Evalue=8e-60,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=30.2884615384615, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44082; Mature: 44082

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMGDTVFMFVTTVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEE
LSFGPDWHGLIGTLDWFGLNGVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHH
FLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTAVNGDGANGLFYGNASLLLKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAYEYTERVN
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCEEEECCCHHHHHHHCCC
>Mature Secondary Structure
MNMGDTVFMFVTTVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEE
LSFGPDWHGLIGTLDWFGLNGVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHH
FLGKRKNINGSSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTAVNGDGANGLFYGNASLLLKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAYEYTERVN
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]