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The map label for this gene is ykfA [H]

Identifier: 218231476

GI number: 218231476

Start: 1367494

End: 1368414

Strand: Reverse

Name: ykfA [H]

Synonym: BCB4264_A1418

Alternate gene names: 218231476

Gene position: 1368414-1367494 (Counterclockwise)

Preceding gene: 218230888

Following gene: 218234826

Centisome position: 25.25

GC content: 36.59

Gene sequence:

>921_bases
ATGATTTATCCAAATGCATTAAAACAAGGTGATACAGTAATGATTATTGCACCATCTGGTCCACCTACAATCGAAAATGT
ATTAAAAGGTGTGAATGTATTACAAGAGATGGGGTTATCCGTAATAATCGGGAAGAGTGTTTACGAGAAATATGGATTTT
TAGCCGGAAGTGATCAAGTTCGGCTTGACGATATACATGAAGCATTTACAAATAATGAAGTGAAGGCAATTTTTTGTGCA
CGAGGTGGTTATGGAAGTGCTCGTCTTCTTCCTCACATTCAATATGAAGTTATTAGGCAGAATCCAAAAATCTTTTGGGG
ATATAGCGATATTACAGCTTTACATACTGCTGTTTCACGTTATGCAAAGCTTATAACATTTCATGGTCCGATGATTGAAG
AACTAGGAAAAGGTATTGATTCCCTATCTTTATCCTCTTTTAATCAGCTATTCCATCCGTATTCAACCATTTTATCTGCA
TCAGAATGTATCGTACCTAGCTCCCACAGTACAGTTACAAGTACGTTAGTTGGAGGTAATTTAGCTGTGTTAGCAAGTAT
AATTGGCTCGCCCTACGAGATAAATACGGACAATACACTTTTATTACTTGAAGACATTGGTGAAGAACCGTACCGCATTG
ACCGTATGTTAAATCAGCTACTTTTATCTGGAAAGTTTAATGAATGCAGGGGCATTATTTTCACAAGTTGTCACGACTGC
ACTCCTTCAAAGCCCTCTCAATCATTGCAAACAATATTATATGAATATTTCGCGCCATATCATATACCTGTCCTATTCGA
TTTACCAATTGGACATATAAGTCCAAACATCGGTATTCCACTTGGAGCTACAGCGACAATAAACACGAATAATAAAACAG
TCTCTATTTCTTCTGGTATAGCCACTTCATATTCAAATTAA

Upstream 100 bases:

>100_bases
TTCCTTTATATCTTCTTTATTTGATAAGCTTTCCCCTGCTTTTGCAAGGGTTGCACTTCATATGAAGCGAATATATTGAA
AGAGAAGGAGGCTGAGCTTA

Downstream 100 bases:

>100_bases
AAAGTAAAAGGAAAAGCGATTACGCTTTTCCTTTTACTTTTACTTTTACTTTTACTTTTACTTTTACTTATAATAATTAA
AAATTCGTCCAGATGCGATA

Product: hypothetical protein

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIYPNALKQGDTVMIIAPSGPPTIENVLKGVNVLQEMGLSVIIGKSVYEKYGFLAGSDQVRLDDIHEAFTNNEVKAIFCA
RGGYGSARLLPHIQYEVIRQNPKIFWGYSDITALHTAVSRYAKLITFHGPMIEELGKGIDSLSLSSFNQLFHPYSTILSA
SECIVPSSHSTVTSTLVGGNLAVLASIIGSPYEINTDNTLLLLEDIGEEPYRIDRMLNQLLLSGKFNECRGIIFTSCHDC
TPSKPSQSLQTILYEYFAPYHIPVLFDLPIGHISPNIGIPLGATATINTNNKTVSISSGIATSYSN

Sequences:

>Translated_306_residues
MIYPNALKQGDTVMIIAPSGPPTIENVLKGVNVLQEMGLSVIIGKSVYEKYGFLAGSDQVRLDDIHEAFTNNEVKAIFCA
RGGYGSARLLPHIQYEVIRQNPKIFWGYSDITALHTAVSRYAKLITFHGPMIEELGKGIDSLSLSSFNQLFHPYSTILSA
SECIVPSSHSTVTSTLVGGNLAVLASIIGSPYEINTDNTLLLLEDIGEEPYRIDRMLNQLLLSGKFNECRGIIFTSCHDC
TPSKPSQSLQTILYEYFAPYHIPVLFDLPIGHISPNIGIPLGATATINTNNKTVSISSGIATSYSN
>Mature_306_residues
MIYPNALKQGDTVMIIAPSGPPTIENVLKGVNVLQEMGLSVIIGKSVYEKYGFLAGSDQVRLDDIHEAFTNNEVKAIFCA
RGGYGSARLLPHIQYEVIRQNPKIFWGYSDITALHTAVSRYAKLITFHGPMIEELGKGIDSLSLSSFNQLFHPYSTILSA
SECIVPSSHSTVTSTLVGGNLAVLASIIGSPYEINTDNTLLLLEDIGEEPYRIDRMLNQLLLSGKFNECRGIIFTSCHDC
TPSKPSQSLQTILYEYFAPYHIPVLFDLPIGHISPNIGIPLGATATINTNNKTVSISSGIATSYSN

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=291, Percent_Identity=30.2405498281787, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 33345; Mature: 33345

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPNALKQGDTVMIIAPSGPPTIENVLKGVNVLQEMGLSVIIGKSVYEKYGFLAGSDQV
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCCCCCCE
RLDDIHEAFTNNEVKAIFCARGGYGSARLLPHIQYEVIRQNPKIFWGYSDITALHTAVSR
EHHHHHHHHCCCCEEEEEEECCCCCCCHHCCHHHHHHHHCCCCEEECCHHHHHHHHHHHH
YAKLITFHGPMIEELGKGIDSLSLSSFNQLFHPYSTILSASECIVPSSHSTVTSTLVGGN
HHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHCCCH
LAVLASIIGSPYEINTDNTLLLLEDIGEEPYRIDRMLNQLLLSGKFNECRGIIFTSCHDC
HHHHHHHHCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHCEEEEECCCCC
TPSKPSQSLQTILYEYFAPYHIPVLFDLPIGHISPNIGIPLGATATINTNNKTVSISSGI
CCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCCCCCCCCCEEEEECCCCEEEEECCC
ATSYSN
CCCCCC
>Mature Secondary Structure
MIYPNALKQGDTVMIIAPSGPPTIENVLKGVNVLQEMGLSVIIGKSVYEKYGFLAGSDQV
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCCCCCCCE
RLDDIHEAFTNNEVKAIFCARGGYGSARLLPHIQYEVIRQNPKIFWGYSDITALHTAVSR
EHHHHHHHHCCCCEEEEEEECCCCCCCHHCCHHHHHHHHCCCCEEECCHHHHHHHHHHHH
YAKLITFHGPMIEELGKGIDSLSLSSFNQLFHPYSTILSASECIVPSSHSTVTSTLVGGN
HHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHCCCH
LAVLASIIGSPYEINTDNTLLLLEDIGEEPYRIDRMLNQLLLSGKFNECRGIIFTSCHDC
HHHHHHHHCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHCEEEEECCCCC
TPSKPSQSLQTILYEYFAPYHIPVLFDLPIGHISPNIGIPLGATATINTNNKTVSISSGI
CCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCCCCCCCCCEEEEECCCCEEEEECCC
ATSYSN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]