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The map label for this gene is proA [H]

Identifier: 218231439

GI number: 218231439

Start: 2864845

End: 2866092

Strand: Reverse

Name: proA [H]

Synonym: BCB4264_A2993

Alternate gene names: 218231439

Gene position: 2866092-2864845 (Counterclockwise)

Preceding gene: 218231374

Following gene: 218232171

Centisome position: 52.89

GC content: 39.02

Gene sequence:

>1248_bases
ATGAATGAAGTGTTGGCAAAGGGGATAAAAGCAAAAGAGGTTGCTAGAGAGCTTGTGCTTAAATCTACAGAGCAAAAAAA
TGAAGCACTTTCCGCAATAGCTGATCAGTTAATATTAGAAACAGCTTATATTTTAGAAGAAAACAAGAGGGATATTGAAG
AAGGTAAGGCAAAAGGATTTTCTGATTCACTCCTTGATCGTCTTCTGCTAACTGAAAATCGTATTATTGATATGACAGAG
GGGATTAAGCAGCTAATTGAATTACGTGATCCTGTAGGAGAATGTGTAAGTGCATGGGAAAGGCCAAATGGACTATCTAT
TCAGGAGATGCGCGTACCACTTGGTGTTATAGGCATGATTTATGAGGCAAGACCGAATGTAACAGTGGATGCAGCTACAA
TTTGTTTGAAGACAGGAAACGCAGTCATATTACGTGGTAGTTCTTCTGCTATTCATTCTAATAAGGCCATCGTTGCCGTT
ATTCACAGAGCGCTCAAACTAACAAGCTTACCTCAAGAAAGCGTACAGCTTATAGAAGATACAACGCGAAATAGCGCGAA
GCAGTTGTTTACAATGAAGGATTATCTAGACGTTCTTATTCCAAGAGGTGGCAAACAATTAATCGATACTGTTGTGAGAG
AAGCGTCTGTTCCAGTACTTGAAACAGGTGCGGGAAATTGTCATATATTCATTGATGAAACAGCAGATAAACAAATGGCG
TTTAATATTATTATAAATGCAAAAACACAGCGTCCATCTGTATGTAATGCAATTGAGACGATTGTACTTCATGAGAATTG
GGCAGAGCAATATGGTAGCGAGTTGTTTTCTTCCTTGAAGGAAAGAGGAGTGGAGCTACGTGGTGATAATAAAGCAGTGG
CGATTGATTCTTCTATCTTACTTGCGACAGAAGAAGACTGGGAAACTGAGTTTTTATCTCTCACACTAGCAGTGAAAGTG
GTTTCCACTGTTGAAGAAGCGATTCATCATATAAATACGTATGGATCTATGCATTCTGAAGCAATTATTACTGAAAACGA
GGAAAACGTCAGCAAGTTTTTTACTTCTGTAGATGCCGCGGCACTTTATCATAATGCATCGACTCGTTTTACAGATGGAT
CCGAATTTGGATTCGGAGCGGAAATTGGTATCAGTACACAAAAGTTACACGTAAGAGGACCAATGGGGCTTCCAGCATTA
ACTTCTACAAAATATGTGATTCGTGGAAATGGGCAAATTCGAAAATAA

Upstream 100 bases:

>100_bases
GGTATGCAAAGCCAAGATATTCAAGTAAGAGGCGAGAGACATAGTTATGAAGTCATCCATAGGGATCATTGGGTTTCACT
TTAAAAAGGAGGAAATAAAA

Downstream 100 bases:

>100_bases
TAAAAGACACTCAATTGGCTTGGGTGTCTTTTATTATAAAAATTAATTACCATACAAATAGCCCAACGAGCATAGCACTT
AATAAAGAAACAGCCATCCC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNEVLAKGIKAKEVARELVLKSTEQKNEALSAIADQLILETAYILEENKRDIEEGKAKGFSDSLLDRLLLTENRIIDMTE
GIKQLIELRDPVGECVSAWERPNGLSIQEMRVPLGVIGMIYEARPNVTVDAATICLKTGNAVILRGSSSAIHSNKAIVAV
IHRALKLTSLPQESVQLIEDTTRNSAKQLFTMKDYLDVLIPRGGKQLIDTVVREASVPVLETGAGNCHIFIDETADKQMA
FNIIINAKTQRPSVCNAIETIVLHENWAEQYGSELFSSLKERGVELRGDNKAVAIDSSILLATEEDWETEFLSLTLAVKV
VSTVEEAIHHINTYGSMHSEAIITENEENVSKFFTSVDAAALYHNASTRFTDGSEFGFGAEIGISTQKLHVRGPMGLPAL
TSTKYVIRGNGQIRK

Sequences:

>Translated_415_residues
MNEVLAKGIKAKEVARELVLKSTEQKNEALSAIADQLILETAYILEENKRDIEEGKAKGFSDSLLDRLLLTENRIIDMTE
GIKQLIELRDPVGECVSAWERPNGLSIQEMRVPLGVIGMIYEARPNVTVDAATICLKTGNAVILRGSSSAIHSNKAIVAV
IHRALKLTSLPQESVQLIEDTTRNSAKQLFTMKDYLDVLIPRGGKQLIDTVVREASVPVLETGAGNCHIFIDETADKQMA
FNIIINAKTQRPSVCNAIETIVLHENWAEQYGSELFSSLKERGVELRGDNKAVAIDSSILLATEEDWETEFLSLTLAVKV
VSTVEEAIHHINTYGSMHSEAIITENEENVSKFFTSVDAAALYHNASTRFTDGSEFGFGAEIGISTQKLHVRGPMGLPAL
TSTKYVIRGNGQIRK
>Mature_415_residues
MNEVLAKGIKAKEVARELVLKSTEQKNEALSAIADQLILETAYILEENKRDIEEGKAKGFSDSLLDRLLLTENRIIDMTE
GIKQLIELRDPVGECVSAWERPNGLSIQEMRVPLGVIGMIYEARPNVTVDAATICLKTGNAVILRGSSSAIHSNKAIVAV
IHRALKLTSLPQESVQLIEDTTRNSAKQLFTMKDYLDVLIPRGGKQLIDTVVREASVPVLETGAGNCHIFIDETADKQMA
FNIIINAKTQRPSVCNAIETIVLHENWAEQYGSELFSSLKERGVELRGDNKAVAIDSSILLATEEDWETEFLSLTLAVKV
VSTVEEAIHHINTYGSMHSEAIITENEENVSKFFTSVDAAALYHNASTRFTDGSEFGFGAEIGISTQKLHVRGPMGLPAL
TSTKYVIRGNGQIRK

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=412, Percent_Identity=32.2815533980583, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI62912457, Length=412, Percent_Identity=32.2815533980583, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1786438, Length=418, Percent_Identity=44.4976076555024, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17569969, Length=408, Percent_Identity=35.0490196078431, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=408, Percent_Identity=35.0490196078431, Blast_Score=262, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6324899, Length=414, Percent_Identity=38.1642512077295, Blast_Score=281, Evalue=1e-76,
Organism=Drosophila melanogaster, GI21357643, Length=413, Percent_Identity=33.1719128329298, Blast_Score=258, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45634; Mature: 45634

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVLAKGIKAKEVARELVLKSTEQKNEALSAIADQLILETAYILEENKRDIEEGKAKGF
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
SDSLLDRLLLTENRIIDMTEGIKQLIELRDPVGECVSAWERPNGLSIQEMRVPLGVIGMI
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHCCHHHHHHH
YEARPNVTVDAATICLKTGNAVILRGSSSAIHSNKAIVAVIHRALKLTSLPQESVQLIED
EECCCCCEEEHEEEEEECCCEEEEECCCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
TTRNSAKQLFTMKDYLDVLIPRGGKQLIDTVVREASVPVLETGAGNCHIFIDETADKQMA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCEEE
FNIIINAKTQRPSVCNAIETIVLHENWAEQYGSELFSSLKERGVELRGDNKAVAIDSSIL
EEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECEEE
LATEEDWETEFLSLTLAVKVVSTVEEAIHHINTYGSMHSEAIITENEENVSKFFTSVDAA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHH
ALYHNASTRFTDGSEFGFGAEIGISTQKLHVRGPMGLPALTSTKYVIRGNGQIRK
HHHCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MNEVLAKGIKAKEVARELVLKSTEQKNEALSAIADQLILETAYILEENKRDIEEGKAKGF
CCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
SDSLLDRLLLTENRIIDMTEGIKQLIELRDPVGECVSAWERPNGLSIQEMRVPLGVIGMI
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHHCCHHHHHHH
YEARPNVTVDAATICLKTGNAVILRGSSSAIHSNKAIVAVIHRALKLTSLPQESVQLIED
EECCCCCEEEHEEEEEECCCEEEEECCCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
TTRNSAKQLFTMKDYLDVLIPRGGKQLIDTVVREASVPVLETGAGNCHIFIDETADKQMA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCEEE
FNIIINAKTQRPSVCNAIETIVLHENWAEQYGSELFSSLKERGVELRGDNKAVAIDSSIL
EEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECEEE
LATEEDWETEFLSLTLAVKVVSTVEEAIHHINTYGSMHSEAIITENEENVSKFFTSVDAA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHH
ALYHNASTRFTDGSEFGFGAEIGISTQKLHVRGPMGLPALTSTKYVIRGNGQIRK
HHHCCCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA