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The map label for this gene is proB [H]

Identifier: 218231374

GI number: 218231374

Start: 2866109

End: 2867212

Strand: Reverse

Name: proB [H]

Synonym: BCB4264_A2994

Alternate gene names: 218231374

Gene position: 2867212-2866109 (Counterclockwise)

Preceding gene: 218232641

Following gene: 218231439

Centisome position: 52.91

GC content: 42.39

Gene sequence:

>1104_bases
GTGAAAAAACAACGAGTTGTTGTAAAGATTGGGAGTAGTTCCTTGGCAGATAGTCACGGAGGCATCTCTACAGAACAGCT
TTCAGATCACGTTGCCGCATTGGCTCGGTTGAAAGAAGATGGGCACGAGGTTGTGTTAATTACATCTGGAGCCGTCGCTG
CAGGTTTTTCAGCGCTAGGATATCCTAGCAGACCTGTAACGATTAAAGGGAAACAGGCTGCAGCAGCTGTCGGACAAAGT
TTGTTAATGCAGGCGTACACCGAGGAATTCCGTAAATATGGCATCGTTACTGCGCAACTTTTGCTGACGAGAAGTGATTT
TTCTAGAAAAGAACAATATAGTAATGCTTACGCTACGTTAGGAGAATTATTAAACCGCTCCGCTCTTCCAATTATTAATG
AAAATGATTCCATCTCCTTAGAGGAGCTGACGTTCGGTGATAATGATATGCTGTCAGCTTTAGTAAGCGGGCTTGTTTCG
GCGGATATGCTTATGATCTTTACGGATGTGAACGGGTTATATGACAAAAATCCTCAGAAAAATGCGGATGCAAAAAAATA
TTATTTTCTTCCTGAAGTTACGGAAGAAATTTCTTCTCTTGCAGGAGATGCTGGCTCAAAACTTGGGACTGGCGGTATGA
AATCAAAAATCGATGCTGCAAAGACAGCGCTCTCTCTTGGTGTGAGTGTATTTATCGGAACAGGACGTGGACAGGAAAAG
TTTGTAGATGTTTTGAAGGGTAAGGGTGATGGAACGTATGTTGGTAATGCTCCTCAAAAAGAAATGAAGATGAACAAGCA
ATGGATTGCCTTACATTCGCTTGTTAGCGGACAAATTGAAGTAGATGCTGGGGCAGCTACTGCCATCATTCAGCATGGTA
AGAGTTTACTTCCAGCTGGTGTTACGAATGTATTAGGATTTTTCCAAGTGGGTGAAGTTGTAGAGGTTGTAACGCAACAA
GGGCGCGTAATAGGAAAAGGACAATGCACATATAGTGCAGAGGAACTAAGAGATGTAAAAGGTATGCAAAGCCAAGATAT
TCAAGTAAGAGGCGAGAGACATAGTTATGAAGTCATCCATAGGGATCATTGGGTTTCACTTTAA

Upstream 100 bases:

>100_bases
TAAATACTTCATTGCAGCATTGATGAGAATAGATATAGTTACAAAGTGAATGAAGTGTAATTTATAAAAAACTTTGATTT
TAGATTTGGGGGATAAAAAA

Downstream 100 bases:

>100_bases
AAAGGAGGAAATAAAAATGAATGAAGTGTTGGCAAAGGGGATAAAAGCAAAAGAGGTTGCTAGAGAGCTTGTGCTTAAAT
CTACAGAGCAAAAAAATGAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKKQRVVVKIGSSSLADSHGGISTEQLSDHVAALARLKEDGHEVVLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQS
LLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVS
ADMLMIFTDVNGLYDKNPQKNADAKKYYFLPEVTEEISSLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSLVSGQIEVDAGAATAIIQHGKSLLPAGVTNVLGFFQVGEVVEVVTQQ
GRVIGKGQCTYSAEELRDVKGMQSQDIQVRGERHSYEVIHRDHWVSL

Sequences:

>Translated_367_residues
MKKQRVVVKIGSSSLADSHGGISTEQLSDHVAALARLKEDGHEVVLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQS
LLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVS
ADMLMIFTDVNGLYDKNPQKNADAKKYYFLPEVTEEISSLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSLVSGQIEVDAGAATAIIQHGKSLLPAGVTNVLGFFQVGEVVEVVTQQ
GRVIGKGQCTYSAEELRDVKGMQSQDIQVRGERHSYEVIHRDHWVSL
>Mature_367_residues
MKKQRVVVKIGSSSLADSHGGISTEQLSDHVAALARLKEDGHEVVLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQS
LLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVS
ADMLMIFTDVNGLYDKNPQKNADAKKYYFLPEVTEEISSLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSLVSGQIEVDAGAATAIIQHGKSLLPAGVTNVLGFFQVGEVVEVVTQQ
GRVIGKGQCTYSAEELRDVKGMQSQDIQVRGERHSYEVIHRDHWVSL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=31.1188811188811, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI62912457, Length=284, Percent_Identity=31.3380281690141, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=39.010989010989, Blast_Score=251, Evalue=4e-68,
Organism=Caenorhabditis elegans, GI17569967, Length=271, Percent_Identity=34.6863468634686, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17569969, Length=271, Percent_Identity=34.6863468634686, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=34.2857142857143, Blast_Score=206, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6321822, Length=419, Percent_Identity=32.9355608591885, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=34.1463414634146, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39371; Mature: 39371

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQRVVVKIGSSSLADSHGGISTEQLSDHVAALARLKEDGHEVVLITSGAVAAGFSALG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHCC
YPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATL
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
GELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQK
HHHHCCCCCCEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
NADAKKYYFLPEVTEEISSLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
CCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHH
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSLVSGQIEVDAGAATAIIQHGKSLLPAG
HHHHHHCCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCCCCH
VTNVLGFFQVGEVVEVVTQQGRVIGKGQCTYSAEELRDVKGMQSQDIQVRGERHSYEVIH
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCCCCCCEEEECCCCCEEEHC
RDHWVSL
CCCCCCC
>Mature Secondary Structure
MKKQRVVVKIGSSSLADSHGGISTEQLSDHVAALARLKEDGHEVVLITSGAVAAGFSALG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHCC
YPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATL
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
GELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQK
HHHHCCCCCCEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
NADAKKYYFLPEVTEEISSLAGDAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEK
CCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCHHH
FVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHSLVSGQIEVDAGAATAIIQHGKSLLPAG
HHHHHHCCCCCCCCCCCCHHHHHHCHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCCCCH
VTNVLGFFQVGEVVEVVTQQGRVIGKGQCTYSAEELRDVKGMQSQDIQVRGERHSYEVIH
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHCCCCCCCEEEECCCCCEEEHC
RDHWVSL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]