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The map label for this gene is amiF [H]

Identifier: 218231260

GI number: 218231260

Start: 3960242

End: 3961240

Strand: Reverse

Name: amiF [H]

Synonym: BCB4264_A4041

Alternate gene names: 218231260

Gene position: 3961240-3960242 (Counterclockwise)

Preceding gene: 218231273

Following gene: 218235488

Centisome position: 73.1

GC content: 36.84

Gene sequence:

>999_bases
ATGGGTAGTAGTGGAAGTATGGTAAAGCCGATTAGTGGTTTTTTGACAGCACTAATTCAATATCCAGTACCAGTAGTAGA
ATCGCGTGCAGACATTGATAAACAAATTCAACAAATCATAAAAACAATTCATTCGACCAAATCAGGTTACCCTGGTTTAG
AATTGATAGTATTTCCTGAATATAGTACACAAGGGCTCAATACAAAAAAATGGACTACAGAAGAATTTTTATGCACAGTG
CCTGGGCCAGAAACAGACTTATTTGCTGAGGCATGTAAAGAATCTAAAGTATATGGTGTTTTTTCAATAATGGAGAAAAA
TCCTGATGGTGGAGAGCCATATAATACAGCAGTTATTATTGATCCACAAGGTGAAATGATTTTGAAGTATCGTAAGCTAA
ATCCTTGGGTGCCAGTCGAGCCTTGGAAAGCTGGAGATTTAGGCTTACCTGTTTGTGATGGACCTGGAGGAAGTAAATTA
GCTGTTTGTATTTGTCATGATGGCATGTTCCCTGAAGTAGCTCGTGAAGCGGCCTATAAAGGTGCAAATGTCTTAATTCG
TATTTCTGGATATAGTACACAAGTTAGTGAACAATGGATGCTAACCAATCGTTCAAATGCATGGCAGAATTTAATGTATA
CATTGTCGGTAAACTTAGCGGGTTATGATGGCGTATTTTATTACTTTGGTGAAGGACAAGTATGTAATTTTGATGGGACT
ACTTTAGTACAGGGACATCGAAATCCTTGGGAGATTGTTACTGCTGAAGTATATCCAGAACTAGCAGATCAGGCTAGATT
AGGATGGGGATTGGAAAATAATATATATAATCTAGGTTCAAGAGGATATGTAGCAACTCCTGGTGGGGTGAAAGAAAATC
CATATACGTTTGTAAAAGATTTAGCTGAAGGTAAATATAAGGTTCCTTGGGAAGATGAGATTAAAGTAAAAGATGGATCT
ATTTATGGATATCCAGTAAAAAAAACGATTCATTCTTAG

Upstream 100 bases:

>100_bases
TCATGCGTAATAATTCCATATGTAGTGCAAGGTTTTTAGAGAGTTACAAATATATATTGTTATCTTTAATAAACACGAAC
TATTTTAAGGGGGAGTTTAA

Downstream 100 bases:

>100_bases
GGAAGGGAAATAAAACAAGCGCATCATATTGATGCGCTTGTTTTTGTTAATCAATAATTTGAAATTCTTTTAACTCTTTT
TCGACATTTTGTAGGAGTTG

Product: formamidase

Products: NA

Alternate protein names: Formamide amidohydrolase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MGSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIQQIIKTIHSTKSGYPGLELIVFPEYSTQGLNTKKWTTEEFLCTV
PGPETDLFAEACKESKVYGVFSIMEKNPDGGEPYNTAVIIDPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKL
AVCICHDGMFPEVAREAAYKGANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGT
TLVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKDLAEGKYKVPWEDEIKVKDGS
IYGYPVKKTIHS

Sequences:

>Translated_332_residues
MGSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIQQIIKTIHSTKSGYPGLELIVFPEYSTQGLNTKKWTTEEFLCTV
PGPETDLFAEACKESKVYGVFSIMEKNPDGGEPYNTAVIIDPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKL
AVCICHDGMFPEVAREAAYKGANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGT
TLVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKDLAEGKYKVPWEDEIKVKDGS
IYGYPVKKTIHS
>Mature_331_residues
GSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIQQIIKTIHSTKSGYPGLELIVFPEYSTQGLNTKKWTTEEFLCTVP
GPETDLFAEACKESKVYGVFSIMEKNPDGGEPYNTAVIIDPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKLA
VCICHDGMFPEVAREAAYKGANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGTT
LVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKDLAEGKYKVPWEDEIKVKDGSI
YGYPVKKTIHS

Specific function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022843
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.49 [H]

Molecular weight: Translated: 36767; Mature: 36636

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIQQIIKTIHSTKSGYPGLELIVFPE
CCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
YSTQGLNTKKWTTEEFLCTVPGPETDLFAEACKESKVYGVFSIMEKNPDGGEPYNTAVII
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEEEE
DPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKLAVCICHDGMFPEVAREAAYK
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHCC
GANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGT
CCEEEEEEECCCCCCCCEEEEECCCCHHHEEEEEEEEEEECCCCEEEEECCCEEECCCCC
TLVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKD
EEEECCCCCCEEEEHHHCHHHHHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHH
LAEGKYKVPWEDEIKVKDGSIYGYPVKKTIHS
HHCCCEECCCCCCEEEECCCEEECCHHHHCCC
>Mature Secondary Structure 
GSSGSMVKPISGFLTALIQYPVPVVESRADIDKQIQQIIKTIHSTKSGYPGLELIVFPE
CCCCCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECC
YSTQGLNTKKWTTEEFLCTVPGPETDLFAEACKESKVYGVFSIMEKNPDGGEPYNTAVII
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEEEE
DPQGEMILKYRKLNPWVPVEPWKAGDLGLPVCDGPGGSKLAVCICHDGMFPEVAREAAYK
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHCC
GANVLIRISGYSTQVSEQWMLTNRSNAWQNLMYTLSVNLAGYDGVFYYFGEGQVCNFDGT
CCEEEEEEECCCCCCCCEEEEECCCCHHHEEEEEEEEEEECCCCEEEEECCCEEECCCCC
TLVQGHRNPWEIVTAEVYPELADQARLGWGLENNIYNLGSRGYVATPGGVKENPYTFVKD
EEEECCCCCCEEEEHHHCHHHHHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHH
LAEGKYKVPWEDEIKVKDGSIYGYPVKKTIHS
HHCCCEECCCCCCEEEECCCEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA