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The map label for this gene is ywcJ [H]

Identifier: 218231089

GI number: 218231089

Start: 1318624

End: 1319475

Strand: Reverse

Name: ywcJ [H]

Synonym: BCB4264_A1359

Alternate gene names: 218231089

Gene position: 1319475-1318624 (Counterclockwise)

Preceding gene: 218231514

Following gene: 218233562

Centisome position: 24.35

GC content: 35.56

Gene sequence:

>852_bases
ATGCTAGAACAAGGACTAGATTATGTTGTCAAACTGGCTAAAAGCAAAAAGAAAATACTAGATACAAACCCAATGCAATA
TTTCATTCGTGCTGCACTTGCGGGTATTTATATCGGTTTTATTATTGTACTATGTTTTAAATTAGGTAACTTTTTTCACA
TTGCAGATTCACCAGCAACTTATTTAGCTGCTTCTATGTTTTTCGGGATTGCTCTCGTACTTATTATATATGGCGGTGCT
GAATTGTTTACTGGGAACACAATGTACTTTACTGTAGCAACTTTAAGAAAAGAAACAACGATTTCTGATACACTACGTAA
CTGGGTTGCTTGTTATGCAGGTAACTTAGCCGGCGCGTTATTCTTTGCCTTACTATTTTACGCAACTGGAATTTTTGAAG
CAATTGATCACGGTCACTTAATGAGTAAAGTCGTTGAAGGAAAGATGAACACACCTACAATGCAGTTATTCTTTAAAGCC
ATTTTATGTAACTGGCTCGTATGTCTTGCTTGTTTCTTACCTTCTCAGGTTAAAGGTGACACAGCAAAAATAATAGTCAT
GATGCTACTTGTTTTCACATTCTTCTTATCTGGTTACGAACATAGTATTGCAAACTTATCACTCTTTGCTTTATCTTTAG
TTTCACCGCATCCTGAGACAATTTCTTTTTCAGGTGCTATTCATAACTTAATTCCTGTTACAATCGGTAACATTATTGGC
GGATCAGTATTTGTCGGTATGGTATACCACTACTTAACAAAGAAAACACCTGAAGTTCAACAGGAAGAAACTGAAATCGC
AGCAGCTCAACAAGAAGAGATTATTCCTTTACAAGCACATATGAAACATTAA

Upstream 100 bases:

>100_bases
GTGAAACATTGCACAAACTTATTGAAAGTGAGAACAATTCCATGTACTTTATTCATATAAGGAGTATTTATAGAAGATTT
ACTAAAGGAGCGTATTACTC

Downstream 100 bases:

>100_bases
AAACTCCAAAGTTTTACTTTGGAGTTTTTAGCTTCCCACGTATTTAACAACAAGGTGAGGATTAATTTCTCCTTCTTCTT
TTCCCTTATCCATTCTCTGT

Product: formate/nitrite transporter family protein

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MLEQGLDYVVKLAKSKKKILDTNPMQYFIRAALAGIYIGFIIVLCFKLGNFFHIADSPATYLAASMFFGIALVLIIYGGA
ELFTGNTMYFTVATLRKETTISDTLRNWVACYAGNLAGALFFALLFYATGIFEAIDHGHLMSKVVEGKMNTPTMQLFFKA
ILCNWLVCLACFLPSQVKGDTAKIIVMMLLVFTFFLSGYEHSIANLSLFALSLVSPHPETISFSGAIHNLIPVTIGNIIG
GSVFVGMVYHYLTKKTPEVQQEETEIAAAQQEEIIPLQAHMKH

Sequences:

>Translated_283_residues
MLEQGLDYVVKLAKSKKKILDTNPMQYFIRAALAGIYIGFIIVLCFKLGNFFHIADSPATYLAASMFFGIALVLIIYGGA
ELFTGNTMYFTVATLRKETTISDTLRNWVACYAGNLAGALFFALLFYATGIFEAIDHGHLMSKVVEGKMNTPTMQLFFKA
ILCNWLVCLACFLPSQVKGDTAKIIVMMLLVFTFFLSGYEHSIANLSLFALSLVSPHPETISFSGAIHNLIPVTIGNIIG
GSVFVGMVYHYLTKKTPEVQQEETEIAAAQQEEIIPLQAHMKH
>Mature_283_residues
MLEQGLDYVVKLAKSKKKILDTNPMQYFIRAALAGIYIGFIIVLCFKLGNFFHIADSPATYLAASMFFGIALVLIIYGGA
ELFTGNTMYFTVATLRKETTISDTLRNWVACYAGNLAGALFFALLFYATGIFEAIDHGHLMSKVVEGKMNTPTMQLFFKA
ILCNWLVCLACFLPSQVKGDTAKIIVMMLLVFTFFLSGYEHSIANLSLFALSLVSPHPETISFSGAIHNLIPVTIGNIIG
GSVFVGMVYHYLTKKTPEVQQEETEIAAAQQEEIIPLQAHMKH

Specific function: Potential Nitrite Transporter. [C]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI87082249, Length=273, Percent_Identity=39.1941391941392, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787132, Length=254, Percent_Identity=31.496062992126, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788837, Length=278, Percent_Identity=30.5755395683453, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321779, Length=190, Percent_Identity=33.1578947368421, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 31277; Mature: 31277

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2163394; 7934828; 9384377 [H]