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The map label for this gene is phaC [H]

Identifier: 218230992

GI number: 218230992

Start: 1325758

End: 1326843

Strand: Direct

Name: phaC [H]

Synonym: BCB4264_A1369

Alternate gene names: 218230992

Gene position: 1325758-1326843 (Clockwise)

Preceding gene: 218232942

Following gene: 218230908

Centisome position: 24.46

GC content: 36.74

Gene sequence:

>1086_bases
ATGACTACATTCGCAACAGAATGGGAAAAGCAATTAGAGCTATACCCAGAAGAGTACCGAAAAGCATACCGCCGAGTGAA
AAGGGCGAGTGAAATTTTATTACGTGAACCAGAGCCGCAAGTCGGATTAACGCCGAAAGAGGTTATTTGGACGAAGAATA
AGACGAAGCTTTATCGCTACATTCCAAAACAAGAAAAAACACAAAGAGTTCCAATTCTGTTAATATATGCTCTTATTAAT
AAACCATATATTATGGATTTAACTCCTGGAAATAGTTTAGTGGAATATCTAGTGGATCGTGGTTTTGATGTATATATGCT
TGATTGGGGCACATTTGGTTTAGAAGATAGTCATTTGAAATTTGATGATTTCGTGTTTGATTATATTGCAAAAGCAGTAA
AAAAAGTAATGCGAACTGCAAAATCGGACGAGATTTCTTTACTTGGTTATTGCATGGGTGGAACGCTAACTTCTATTTAT
GCAGCGCTTCATCCGCACATGCCAATTCGTAATTTAATTTTCATGACAAGTCCTTTTGATTTCTCTGAAACAGGATTGTA
TGGTCCTTTATTAGATGAGAAATACTTCAATTTAGATAAAGCGGTTGATACATTTGGAAATATTCCGCCAGAAATGATTG
ATTTCGGAAACAAAATGTTAAAGCCAATTACGAACTTTGTTGGTCCATATGTTGCTCTAGTAGATCGTTCAGAGAATGAA
CGCTTCGTCGAAAGCTGGAGATTGGTTCAAAAGTGGGTTGGTGATGGTATTCCGTTCCCAGGTGAATCATACAGACAGTG
GATTCGTGATTTTTATCAAAATAATAAATTGGTTAAAGGTGAACTCGTTATTCGCGGACAAAAGGTAGATCTTGCAAATA
TTAAGGCGAATGTCTTAAATATTTCCGGGAAACGTGATCATATCGCTTTGCCATGTCAAGTAGAAGCATTACTAAACCAT
ATTTCTAGCACAGATAAACAATATGTATGTTTACCGACAGGGCATATGTCTATCGTTTACGGTGGAACAGCTGTAAAACA
AACATATCCGACGATTGGAAATTGGCTTGAAGAGCGTTCTAATTAA

Upstream 100 bases:

>100_bases
CGGATTATATATGTAATGAAGTAAGAAAAAAGTGCATATCCATAGCAGGAATGCACTTCTTTTTTTTAAGAAGGAAATCG
ACCAAAAAGGAGATAGAAAA

Downstream 100 bases:

>100_bases
AAATAAAAAATCCAACTAGCCTATGTTAGTTGGATTTTTTTATGAAAAATAACAAAACATAGAAAGGAGAAATTTATATT
TTAGGAGTATAATTATGAAA

Product: poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRYIPKQEKTQRVPILLIYALIN
KPYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLKFDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIY
AALHPHMPIRNLIFMTSPFDFSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENE
RFVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLNISGKRDHIALPCQVEALLNH
ISSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGNWLEERSN

Sequences:

>Translated_361_residues
MTTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRYIPKQEKTQRVPILLIYALIN
KPYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLKFDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIY
AALHPHMPIRNLIFMTSPFDFSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENE
RFVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLNISGKRDHIALPCQVEALLNH
ISSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGNWLEERSN
>Mature_360_residues
TTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRYIPKQEKTQRVPILLIYALINK
PYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLKFDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIYA
ALHPHMPIRNLIFMTSPFDFSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENER
FVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLNISGKRDHIALPCQVEALLNHI
SSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGNWLEERSN

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010125 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 41680; Mature: 41548

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRY
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCHHHHHC
IPKQEKTQRVPILLIYALINKPYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLK
CCCCCCCCCCHHHEEEHHHCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
FDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIYAALHPHMPIRNLIFMTSPFD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCHHHEEEEECCCC
FSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCH
RFVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCEEHHHCEEEEEE
ISGKRDHIALPCQVEALLNHISSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGNWLEERS
ECCCCCCEECCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHHHHCCHHHHHHHHCC
N
C
>Mature Secondary Structure 
TTFATEWEKQLELYPEEYRKAYRRVKRASEILLREPEPQVGLTPKEVIWTKNKTKLYRY
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEECCCHHHHHC
IPKQEKTQRVPILLIYALINKPYIMDLTPGNSLVEYLVDRGFDVYMLDWGTFGLEDSHLK
CCCCCCCCCCHHHEEEHHHCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
FDDFVFDYIAKAVKKVMRTAKSDEISLLGYCMGGTLTSIYAALHPHMPIRNLIFMTSPFD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCHHHEEEEECCCC
FSETGLYGPLLDEKYFNLDKAVDTFGNIPPEMIDFGNKMLKPITNFVGPYVALVDRSENE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCH
RFVESWRLVQKWVGDGIPFPGESYRQWIRDFYQNNKLVKGELVIRGQKVDLANIKANVLN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCEEHHHCEEEEEE
ISGKRDHIALPCQVEALLNHISSTDKQYVCLPTGHMSIVYGGTAVKQTYPTIGNWLEERS
ECCCCCCEECCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHHHHCCHHHHHHHHCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1396692 [H]