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The map label for this gene is pfl [H]

Identifier: 218230943

GI number: 218230943

Start: 497412

End: 499661

Strand: Direct

Name: pfl [H]

Synonym: BCB4264_A0512

Alternate gene names: 218230943

Gene position: 497412-499661 (Clockwise)

Preceding gene: 218231693

Following gene: 218233688

Centisome position: 9.18

GC content: 35.82

Gene sequence:

>2250_bases
ATGACTCAAGTATTAGAAAATGTTAAAAACGCATGGGAAAACTTTAAAGGTGAAAAATGGAAAGCAGAGATTGATGTTCG
CGATTTCATTTTAAATAATGTAAACGTTTTCGAAGGGGACGAATCTTTCTTAGCAGAAGCAACTGAAGCAACGAAACAAC
TTTGGGATCAAGTAATGGATTTAACAACGAAAGAACGTGAAAACGGTGGCGTTCTTGATATGGATACAAAAATTGTTTCT
TCTATTACATCACATGACCCAGGATACTTAAATAAAGATATTGAAAAAGTAGTCGGTTTCCAAACTGATAAACCATTTAA
ACGTTCTTTACAACCATATGGTGGTATTCGTATGGCAGAACAAGCTTGTGAATCTTATGGATACGAAATGGATAAAGAAC
TTAGTCGTATTTTTAGAGAGTGGAGAAAAACTCATAACCAAGGTGTATTTGATGCATACACACCAGAAATGAGAAATGCA
CGTAAATCAGGTGTTATTACTGGTCTTCCAGATGCATACGGACGTGGACGTATTATCGGTGACTATCGCCGAGTAGCGTT
ATATGGTATTGATCATTTAATTGAAGCGAAAAAAGCTGATTTGAATTTAACTGGTGGTGTAATGAGCGAAGATACGATGC
GTTTACGCGAAGAGTTATCTGAGCAAATGCGTGCACTTCAAGAGTTAAAAGAAATGGCTGCTTCTCATGGCTTCGATATT
TCTAAGCCAGCAACAAATGCACAAGAAGCATTCCAATGGTTATACTTCGCATACCTTGCAGCGATTAAAGAACAAAACGG
GGCTGCAATGAGTCTTGGACGTACTTCTACATTCTTAGATATTTACATTGAAAGAGATTTAGCAAATGGTACTTTAACAG
AAGAAGAAGTACAAGAAATTGTAGATCACTTCATTATGAAATTACGTCTTGTGAAATTCGCAAGAACACCTGATTATAAT
GAATTATTCTCTGGTGACCCAACTTGGGTAACTGAATCTATCGGTGGTATGGCATTAGATGGTCGTCCATTAGTAACAAA
GAACTCATTCCGTTTCTTGCATACATTAGATAATTTAGGACCAGCTCCAGAACCAAACTTAACTGTTCTTTGGTCTAAAC
AATTACCAGAGAACTTTAAAAACTACTGTGCGAAAATGTCTATTAAAACATCAGCAATTCAATATGAAAATGATGACATT
ATGCGTCCTGAATACGGCGATGATTACGGTATTGCATGTTGCGTATCTGCAATGAGAATCGGTAAACAAATGCAGTTCTT
CGGAGCTCGTGCAAACTTAGCGAAAGCATTATTATATGCGATTAACGGTGGTAAAGATGAAAAGTCAAAAGCACAAGTTG
GTCCTGAGTACGCACCAATTACTTCTGAAGTATTAAATTATGAAGAAGTTATGCATAAGTTTGATATGACAATGGAATGG
TTAGCGGGTCTATATTTAAACACATTAAATGTTATCCATTACATGCATGATAAATATAGCTATGAACGTATTGAAATGGC
ACTTCATGATACAAACGTTCTTCGTACAATGGCAACAGGTATCGCTGGTCTATCTGTAGTAGCAGATTCATTAAGTGCAA
TTAAATACGCAAAAGTAAAACCAATTCGTGATGAAAATGGTATTGCAGTTGACTTCGAGATTGAAGGAGATTTCCCTAAA
TACGGTAACAATGATGATCGTGTAGATGAGATTGCTGTAAATCTTGTAAAAACATTTATGAATAAACTTCGTAAACATAA
AACATACAGAAATTCTGTTCATACAATGTCAATCTTAACAATCACATCTAATGTTGTATACGGTAAGAAAACTGGTAATA
CTCCAGATGGCCGCCGTACTGGAGAACCATTTGCACCAGGTGCGAACCCAATGCATGGACGTGATACAAAAGGTGCATTA
GCTTCATTATTATCTGTGGCTAAATTACCATATGAAGATGCACAAGATGGTATTTCTAATACGTTCTCTATTATTCCGAA
AGCACTTGGTAAAGAAGATGATGTACAAGTACGTAACTTAGTATCAATGCTTGATGGTTATGCAGTAAAAGAAGGGCACC
ACTTAAATATTAACGTATTTAACCGTGAAACATTAATGGATGCAATGGAACATCCTGAGAAGTATCCACAATTAACAATT
CGTGTATCTGGTTATGCTGTAAACTTTATTAAATTAACTCGTGAACAACAAATTGATGTAATTAACCGTACAATGCATGA
AAGCATGTAA

Upstream 100 bases:

>100_bases
ACTATTTCGTTCACTTTCGTAAAAAAGTAGTATTCTAAGTGTGTAAGCTGTTATATAAAAAATCTTAGTCCTGTACTAAT
CAAAAATGGAGGAGATTAGG

Downstream 100 bases:

>100_bases
ATGAAAAAAGGTGTCCCTTCGCTCTTAATATATAGCGAAGGGAGGCTGTTTCAATAAAGGAGGAAGTAACATGGTAAAAG
GAAGAATACATTCTGTAGAG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVFEGDESFLAEATEATKQLWDQVMDLTTKERENGGVLDMDTKIVS
SITSHDPGYLNKDIEKVVGFQTDKPFKRSLQPYGGIRMAEQACESYGYEMDKELSRIFREWRKTHNQGVFDAYTPEMRNA
RKSGVITGLPDAYGRGRIIGDYRRVALYGIDHLIEAKKADLNLTGGVMSEDTMRLREELSEQMRALQELKEMAASHGFDI
SKPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEEVQEIVDHFIMKLRLVKFARTPDYN
ELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPENFKNYCAKMSIKTSAIQYENDDI
MRPEYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLNYEEVMHKFDMTMEW
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIEGDFPK
YGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGAL
ASLLSVAKLPYEDAQDGISNTFSIIPKALGKEDDVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTI
RVSGYAVNFIKLTREQQIDVINRTMHESM

Sequences:

>Translated_749_residues
MTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVFEGDESFLAEATEATKQLWDQVMDLTTKERENGGVLDMDTKIVS
SITSHDPGYLNKDIEKVVGFQTDKPFKRSLQPYGGIRMAEQACESYGYEMDKELSRIFREWRKTHNQGVFDAYTPEMRNA
RKSGVITGLPDAYGRGRIIGDYRRVALYGIDHLIEAKKADLNLTGGVMSEDTMRLREELSEQMRALQELKEMAASHGFDI
SKPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEEVQEIVDHFIMKLRLVKFARTPDYN
ELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPENFKNYCAKMSIKTSAIQYENDDI
MRPEYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLNYEEVMHKFDMTMEW
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIEGDFPK
YGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGAL
ASLLSVAKLPYEDAQDGISNTFSIIPKALGKEDDVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTI
RVSGYAVNFIKLTREQQIDVINRTMHESM
>Mature_748_residues
TQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVFEGDESFLAEATEATKQLWDQVMDLTTKERENGGVLDMDTKIVSS
ITSHDPGYLNKDIEKVVGFQTDKPFKRSLQPYGGIRMAEQACESYGYEMDKELSRIFREWRKTHNQGVFDAYTPEMRNAR
KSGVITGLPDAYGRGRIIGDYRRVALYGIDHLIEAKKADLNLTGGVMSEDTMRLREELSEQMRALQELKEMAASHGFDIS
KPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEEVQEIVDHFIMKLRLVKFARTPDYNE
LFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSKQLPENFKNYCAKMSIKTSAIQYENDDIM
RPEYGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLNYEEVMHKFDMTMEWL
AGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVKPIRDENGIAVDFEIEGDFPKY
GNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILTITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGALA
SLLSVAKLPYEDAQDGISNTFSIIPKALGKEDDVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTIR
VSGYAVNFIKLTREQQIDVINRTMHESM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=760, Percent_Identity=65.6578947368421, Blast_Score=1061, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=751, Percent_Identity=65.7789613848202, Blast_Score=1032, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=574, Percent_Identity=27.8745644599303, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1790388, Length=734, Percent_Identity=24.6594005449591, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1788933, Length=83, Percent_Identity=57.8313253012048, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84709; Mature: 84578

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVFEGDESFLAEATEATKQLWDQVMD
CHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHH
LTTKERENGGVLDMDTKIVSSITSHDPGYLNKDIEKVVGFQTDKPFKRSLQPYGGIRMAE
HHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHCCCCCCCHHHHH
QACESYGYEMDKELSRIFREWRKTHNQGVFDAYTPEMRNARKSGVITGLPDAYGRGRIIG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEECCCCCCCCCCEEH
DYRRVALYGIDHLIEAKKADLNLTGGVMSEDTMRLREELSEQMRALQELKEMAASHGFDI
HHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEEVQEI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHH
VDHFIMKLRLVKFARTPDYNELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLG
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCCEEECCCCEEECCCHHHHHHHHHCC
PAPEPNLTVLWSKQLPENFKNYCAKMSIKTSAIQYENDDIMRPEYGDDYGIACCVSAMRI
CCCCCCEEEEECHHCCHHHHHHHHHCCEEHHEEEECCCCCCCCCCCCCCHHHHHHHHHHH
GKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLNYEEVMHKFDMTMEW
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIRDENGIAVDFEIEGDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILT
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEE
ITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGALASLLSVAKLPYEDAQDGISN
EECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
TFSIIPKALGKEDDVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTI
HHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCCCCCCEEEE
RVSGYAVNFIKLTREQQIDVINRTMHESM
EEECEEEEEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQVLENVKNAWENFKGEKWKAEIDVRDFILNNVNVFEGDESFLAEATEATKQLWDQVMD
HHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHH
LTTKERENGGVLDMDTKIVSSITSHDPGYLNKDIEKVVGFQTDKPFKRSLQPYGGIRMAE
HHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHCCCCCCCHHHHH
QACESYGYEMDKELSRIFREWRKTHNQGVFDAYTPEMRNARKSGVITGLPDAYGRGRIIG
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCEECCCCCCCCCCEEH
DYRRVALYGIDHLIEAKKADLNLTGGVMSEDTMRLREELSEQMRALQELKEMAASHGFDI
HHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SKPATNAQEAFQWLYFAYLAAIKEQNGAAMSLGRTSTFLDIYIERDLANGTLTEEEVQEI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHH
VDHFIMKLRLVKFARTPDYNELFSGDPTWVTESIGGMALDGRPLVTKNSFRFLHTLDNLG
HHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCCCCEEECCCCEEECCCHHHHHHHHHCC
PAPEPNLTVLWSKQLPENFKNYCAKMSIKTSAIQYENDDIMRPEYGDDYGIACCVSAMRI
CCCCCCEEEEECHHCCHHHHHHHHHCCEEHHEEEECCCCCCCCCCCCCCHHHHHHHHHHH
GKQMQFFGARANLAKALLYAINGGKDEKSKAQVGPEYAPITSEVLNYEEVMHKFDMTMEW
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LAGLYLNTLNVIHYMHDKYSYERIEMALHDTNVLRTMATGIAGLSVVADSLSAIKYAKVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIRDENGIAVDFEIEGDFPKYGNNDDRVDEIAVNLVKTFMNKLRKHKTYRNSVHTMSILT
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEE
ITSNVVYGKKTGNTPDGRRTGEPFAPGANPMHGRDTKGALASLLSVAKLPYEDAQDGISN
EECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
TFSIIPKALGKEDDVQVRNLVSMLDGYAVKEGHHLNINVFNRETLMDAMEHPEKYPQLTI
HHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHHCCCCCCEEEE
RVSGYAVNFIKLTREQQIDVINRTMHESM
EEECEEEEEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA