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The map label for this gene is truA [H]

Identifier: 218230830

GI number: 218230830

Start: 143883

End: 144641

Strand: Direct

Name: truA [H]

Synonym: BCB4264_A0163

Alternate gene names: 218230830

Gene position: 143883-144641 (Clockwise)

Preceding gene: 218233106

Following gene: 218231560

Centisome position: 2.66

GC content: 34.78

Gene sequence:

>759_bases
ATGGAGAATAGAGAAATGGATAGAATAAAATGTACGGTTGCATATGATGGCATGCATTTTTGTGGTTATCAAATTCAGCC
GAACCATCGTACTGTTCAACAGGAGATAGAAAAAGCATTGCAGAAATTGCATAAGGGAGAACTTGTTCGTGTTCAGGCAT
CTGGTAGAACAGATTCTACAGTTCATGCAAAAGGACAAGTAATACACTTTGATACTCCTTTGTCTCTTGAAGAATGGCAA
TGGATTAATGCTTTAAACACAATGTTGCCAGATGATATTGTTATTAGGCAAGTAGAGAAAAAAACAGAAGAATTTCATGC
ACGTTACGGTGTTGAGAAAAAAGAATATCGTTATCGTGTATTATTATCAAAGACTGCGGATGTATTCCGTAGAAATTACG
TATATCAATATCCATACCCGCTCGAAATCAACTCTATAAGAAAAGCAATTCCTTATTTTATTGGAACACATGATTTCACT
TCTTTTTGTTCGGCAAAAACTGACAAAAAAGATAAAGTGCGAACAATTTATGAAATCGAGTTAATTGAACAAAATGATGA
AATAATTTTTCGTTTTGTAGGTAATGGATTTTTATATAATATGGTCAGAATTATTGTTGGTACGTTACTTAGCGTAGGGC
AAGGAAAGCTTGATCCTGATAGCATTCCAGAGATTTTAGCGAAACAAAATCGTCAGTTTGCTGGAAAGATGGCTCCAGGT
CATGGGCTTTACTTATGGGAGGTAAACTATAACAACTAA

Upstream 100 bases:

>100_bases
GACGTACTAAATTTAGGCAACTACGCTGGAAAACAAGCGATACAGTAACGATTGTCAGCTTATTTTGTTTAGCTTGTATT
TTGGTATGGGTGCGATCGTA

Downstream 100 bases:

>100_bases
TCCTGGTGTAACATTTGCTTGACATTAGAAACTCAAAAGTATATCATAACATATGGTATTGTTTCTAAAACCACGATTAG
CCCCGGAAGGAAATCGTGTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1 [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MENREMDRIKCTVAYDGMHFCGYQIQPNHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQ
WINALNTMLPDDIVIRQVEKKTEEFHARYGVEKKEYRYRVLLSKTADVFRRNYVYQYPYPLEINSIRKAIPYFIGTHDFT
SFCSAKTDKKDKVRTIYEIELIEQNDEIIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPG
HGLYLWEVNYNN

Sequences:

>Translated_252_residues
MENREMDRIKCTVAYDGMHFCGYQIQPNHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQ
WINALNTMLPDDIVIRQVEKKTEEFHARYGVEKKEYRYRVLLSKTADVFRRNYVYQYPYPLEINSIRKAIPYFIGTHDFT
SFCSAKTDKKDKVRTIYEIELIEQNDEIIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPG
HGLYLWEVNYNN
>Mature_252_residues
MENREMDRIKCTVAYDGMHFCGYQIQPNHRTVQQEIEKALQKLHKGELVRVQASGRTDSTVHAKGQVIHFDTPLSLEEWQ
WINALNTMLPDDIVIRQVEKKTEEFHARYGVEKKEYRYRVLLSKTADVFRRNYVYQYPYPLEINSIRKAIPYFIGTHDFT
SFCSAKTDKKDKVRTIYEIELIEQNDEIIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPG
HGLYLWEVNYNN

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=250, Percent_Identity=31.2, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=32.5102880658436, Blast_Score=147, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321112, Length=239, Percent_Identity=26.7782426778243, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI116007752, Length=304, Percent_Identity=25.9868421052632, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281362146, Length=274, Percent_Identity=24.4525547445255, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648455, Length=274, Percent_Identity=24.8175182481752, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29472; Mature: 29472

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENREMDRIKCTVAYDGMHFCGYQIQPNHRTVQQEIEKALQKLHKGELVRVQASGRTDST
CCCCCCCEEEEEEEECCCEEECEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
VHAKGQVIHFDTPLSLEEWQWINALNTMLPDDIVIRQVEKKTEEFHARYGVEKKEYRYRV
EECCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHEEH
LLSKTADVFRRNYVYQYPYPLEINSIRKAIPYFIGTHDFTSFCSAKTDKKDKVRTIYEIE
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHEEEEEEE
LIEQNDEIIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPG
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCCCC
HGLYLWEVNYNN
CEEEEEEEECCC
>Mature Secondary Structure
MENREMDRIKCTVAYDGMHFCGYQIQPNHRTVQQEIEKALQKLHKGELVRVQASGRTDST
CCCCCCCEEEEEEEECCCEEECEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
VHAKGQVIHFDTPLSLEEWQWINALNTMLPDDIVIRQVEKKTEEFHARYGVEKKEYRYRV
EECCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHEEH
LLSKTADVFRRNYVYQYPYPLEINSIRKAIPYFIGTHDFTSFCSAKTDKKDKVRTIYEIE
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHEEEEEEE
LIEQNDEIIFRFVGNGFLYNMVRIIVGTLLSVGQGKLDPDSIPEILAKQNRQFAGKMAPG
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCCCC
HGLYLWEVNYNN
CEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]