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The map label for this gene is ligA [H]

Identifier: 218230787

GI number: 218230787

Start: 327048

End: 329057

Strand: Direct

Name: ligA [H]

Synonym: BCB4264_A0352

Alternate gene names: 218230787

Gene position: 327048-329057 (Clockwise)

Preceding gene: 218231540

Following gene: 218233218

Centisome position: 6.04

GC content: 37.66

Gene sequence:

>2010_bases
ATGTCAAAAGAGATAGCAAAAAAACGTATTGAAGAACTGCGTGATTTATTAAATACATTTAACTATCAATATCACGTATT
AGACAATCCTTCTGTTTCTGATGCGGAATATGACCGTAATATGCAGGAGCTTATAAAATTAGAAGCAGAGAACCCAGAGT
TTATGAGTGAAGATTCTCCCTCCGTTCGCGTTGGGGGGACTGTTCTTGATATATTTGAAAAAGTAACACATAAGTCACCA
ATGTTAAGTTTAGGAAATGCATTTAACGAAGGAGATTTACGTGATTTCGACAGAAGAGTACGTCAAGGAATTGATGGTGC
GAATGTAAGATATATATGTGAATTAAAAATTGACGGGCTTGCCGTTTCGCTTCATTATGAAAAAGGACGCTTCATTCAAG
GGGCGACACGTGGTGATGGTGTAACGGGTGAAGATATTACTCAAAATTTAAAAACGATTAAAGCTATCCCACTTCGTTTA
AATGAAGAAGTAACGTTAGAAGCACGAGGCGAAGCTTATATGCCGAAGCGTTCATTCGTTAAACTAAATGAAGAAAAAGA
GCAAAATGGAGAAGATGTATTTGCGAATCCGCGTAATGCAGCAGCAGGTTCAATACGTCAACTTGATCCGAAAATTGCAG
CGAAGCGTAACTTATCTATGTTTGTGTATGGTCTTGCGAATGTAGAAGAAAAAACAATTCCATCACATAGTGAATCGCTT
GATTTCTTAGGTGAACTTGGATTTAAGACAAATCCAAATCGCCGTACATGTGAAACAATTGAAGAAGTTATAGCTTATGT
AGAAGAATGGCAAGAAAAACGTCCGCATCTTGATTATGAGATTGATGGAATCGTTATAAAAGTAGATGATGTTGCCCTTC
AAGAAAGTCTAGGAACTACAGCGAAGAGTCCAAGATGGGCGATTGCTTATAAATTCCCGGCTGAAGAAGTTGTAACGAGA
TTAACAGGCATTGAATTAAGTGTTGGCCGAACAGGAGTTGTAACACCGACTGCAGAACTAGAGCCAGTGAGAGTTGCTGG
TACGATTGTTCGTCGTGCTTCTTTACATAACGAAGATTTAATTCGTGAAAAAGACATTCGAATTGGTGACTACGTTGTTG
TGAAGAAGGCTGGAGATATTATTCCTGAAGTTGTGAACGTTATTTTTGATAAGCGTACTGGTGAGGAAGAAGAATACCGC
ATGCCAACTCATTGTCCAGCATGTGAGAGTGAACTTGTTCGTTTAGAAGAAGAAGTAGCACTTCGTTGTATAAATCCAAC
TTGTCCTGCTCAAATTCGAGAAGGGTTAATCCATTTCGTTTCAAGAAATGCAATGAATATTGATGGACTTGGGGAACGTG
TCATTACACAACTCTTTGATGCTGATTACATTCGTACATTTGCTGATTTATATGCATTGACGAAAGAGCAATTATTACAG
TTAGAACGTTTTGGTGAAAAATCAGCAACAAACTTAATACAAGCAATTGAAAATTCTAAAGAAAACTCGTTAGAGCGATT
ATTATTTGGTCTTGGAATTCGCCACGTTGGAGCGAAAGCAGCACGTACATTTGCAGAGCACTTTGAAACGATGGATGAGC
TTGTAAAAGCAACGGAAGAAGAACTAAAAGCAATTAATGAAATTGGCGAAAAAATGGCTCAATCTGTTGTGACATATTTT
GATAATGAAGATGTATTAGAGTTATTACAGCAGTTTAAAGAGTATGGCGTGAATATGAAATACAAAGGTATAAAAATTGC
AGATTTACAAAATGTTGAATCTTACTTCGCAGGAAAAACGGTTGTTTTAACAGGTAAGTTAGAAGTTATGGGACGAAGTG
AAGCGAAGAAGAAGATTGAGGCATTAGGTGGAAAAGTAACGGGAAGTGTTAGTAAGAGTACGGATTTAGTTGTTGCAGGT
GAAGCGGCTGGTTCGAAATTAGCACAAGCGGAGAAACATAATGTTGAGGTTTGGAATGAAGAGAGGTTCTTACAAGAGCT
AAATAAGTAA

Upstream 100 bases:

>100_bases
ATGCAAAAGAATTAGATATTGCATTCCCAAGCCCAATTGGTGTTAAACGTTTGTTAGCAAAATTTGCACCTGTGACGAAA
CAATAGAAAGGAATGAGGAT

Downstream 100 bases:

>100_bases
GAGGTGCAAACTTACCATGAAAAAAATAGCATTAGCGGTATTAAGCCTTGGCCTACTTGTAAGTGGGTGTAGTGCAGGTG
CCGACAAAGATGAAAAAGTG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSPSVRVGGTVLDIFEKVTHKSP
MLSLGNAFNEGDLRDFDRRVRQGIDGANVRYICELKIDGLAVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRL
NEEVTLEARGEAYMPKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESL
DFLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTTAKSPRWAIAYKFPAEEVVTR
LTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGEEEEYR
MPTHCPACESELVRLEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYALTKEQLLQ
LERFGEKSATNLIQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDELVKATEEELKAINEIGEKMAQSVVTYF
DNEDVLELLQQFKEYGVNMKYKGIKIADLQNVESYFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAG
EAAGSKLAQAEKHNVEVWNEERFLQELNK

Sequences:

>Translated_669_residues
MSKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSPSVRVGGTVLDIFEKVTHKSP
MLSLGNAFNEGDLRDFDRRVRQGIDGANVRYICELKIDGLAVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRL
NEEVTLEARGEAYMPKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESL
DFLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTTAKSPRWAIAYKFPAEEVVTR
LTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGEEEEYR
MPTHCPACESELVRLEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYALTKEQLLQ
LERFGEKSATNLIQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDELVKATEEELKAINEIGEKMAQSVVTYF
DNEDVLELLQQFKEYGVNMKYKGIKIADLQNVESYFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAG
EAAGSKLAQAEKHNVEVWNEERFLQELNK
>Mature_668_residues
SKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSPSVRVGGTVLDIFEKVTHKSPM
LSLGNAFNEGDLRDFDRRVRQGIDGANVRYICELKIDGLAVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRLN
EEVTLEARGEAYMPKRSFVKLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESLD
FLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTTAKSPRWAIAYKFPAEEVVTRL
TGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDLIREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGEEEEYRM
PTHCPACESELVRLEEEVALRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYALTKEQLLQL
ERFGEKSATNLIQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDELVKATEEELKAINEIGEKMAQSVVTYFD
NEDVLELLQQFKEYGVNMKYKGIKIADLQNVESYFAGKTVVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAGE
AAGSKLAQAEKHNVEVWNEERFLQELNK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=46.6467958271237, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI87082305, Length=460, Percent_Identity=23.695652173913, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75227; Mature: 75096

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHHHCCCCCCCCCCCCC
SVRVGGTVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDGANVRYICELKIDGL
CEEECHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEECCE
AVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRLNEEVTLEARGEAYMPKRSFV
EEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCEECCCEEEEEECCCCCCCCHHHE
KLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESL
ECCCHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCEEEEEEECCCCHHCCCCCCHHH
DFLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCHHHHHHCCCC
AKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDL
CCCCCEEEEEECCHHHHHHHHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCHHH
IREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGEEEEYRMPTHCPACESELVRLEEEVA
HHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHH
LRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYALTKEQLLQ
HEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERFGEKSATNLIQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDELVKATEE
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKAINEIGEKMAQSVVTYFDNEDVLELLQQFKEYGVNMKYKGIKIADLQNVESYFAGKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCEEEECHHHHHHHHCCCE
VVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAGEAAGSKLAQAEKHNVEVWNE
EEEEEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCHHHHHHHHCCCEEECH
ERFLQELNK
HHHHHHHCC
>Mature Secondary Structure 
SKEIAKKRIEELRDLLNTFNYQYHVLDNPSVSDAEYDRNMQELIKLEAENPEFMSEDSP
CHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCHHHHHHHCCCCCCCCCCCCC
SVRVGGTVLDIFEKVTHKSPMLSLGNAFNEGDLRDFDRRVRQGIDGANVRYICELKIDGL
CEEECHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEEECCE
AVSLHYEKGRFIQGATRGDGVTGEDITQNLKTIKAIPLRLNEEVTLEARGEAYMPKRSFV
EEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHCCEECCCEEEEEECCCCCCCCHHHE
KLNEEKEQNGEDVFANPRNAAAGSIRQLDPKIAAKRNLSMFVYGLANVEEKTIPSHSESL
ECCCHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHHCCCEEEEEEECCCCHHCCCCCCHHH
DFLGELGFKTNPNRRTCETIEEVIAYVEEWQEKRPHLDYEIDGIVIKVDDVALQESLGTT
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCHHHHHHCCCC
AKSPRWAIAYKFPAEEVVTRLTGIELSVGRTGVVTPTAELEPVRVAGTIVRRASLHNEDL
CCCCCEEEEEECCHHHHHHHHHCCEEECCCCCEECCCCCCCCHHHHHHHHHHHHCCCHHH
IREKDIRIGDYVVVKKAGDIIPEVVNVIFDKRTGEEEEYRMPTHCPACESELVRLEEEVA
HHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHH
LRCINPTCPAQIREGLIHFVSRNAMNIDGLGERVITQLFDADYIRTFADLYALTKEQLLQ
HEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERFGEKSATNLIQAIENSKENSLERLLFGLGIRHVGAKAARTFAEHFETMDELVKATEE
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKAINEIGEKMAQSVVTYFDNEDVLELLQQFKEYGVNMKYKGIKIADLQNVESYFAGKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCEEEECHHHHHHHHCCCE
VVLTGKLEVMGRSEAKKKIEALGGKVTGSVSKSTDLVVAGEAAGSKLAQAEKHNVEVWNE
EEEEEEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCHHHHHHHHCCCEEECH
ERFLQELNK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA