Click here to switch to the map view.

The map label for this gene is regA [H]

Identifier: 213693289

GI number: 213693289

Start: 2744077

End: 2745093

Strand: Direct

Name: regA [H]

Synonym: Blon_2443

Alternate gene names: 213693289

Gene position: 2744077-2745093 (Clockwise)

Preceding gene: 213693285

Following gene: 213693292

Centisome position: 96.87

GC content: 60.67

Gene sequence:

>1017_bases
ATGGCTACGAGCATTCAGGATGTCGCCCGCGAGGCCGGCGTCTCCATCTCCACCGTCTCACGTTCCTTCACCAGACCGGA
TCTGGTGTCGGCCAAGACCCGCGAGCGCGTGCTCGCCATCGCCGACAAACTCAATTTCTCACTCTCCCGCTCGGCTGCGG
CCCTGAAATCCGGCCGCGCCCTGCGCATCGCAGTACTGATGAGCGGCCATATCCGCCTGTGGTTCACCGCCTCGGTCATC
GAGGGGCTCAACGAGGTGCTGCACACGCAGGGCTACGATATTTCGGTATTCCAGATTTCCAGCATCGAGGAGCGCAAGGA
ATTCTTCGAGATGTTGCCGGTACGCCGTAACGCCGATGCCGTGATTGTGGCGTCTTTCGATATCGACAACAACGAAATCG
CGCAACTGGCTTCGGTCGGCGTGCCGATCGTCGGCATCAATTCAGTCGAGCCCGAAGCCCGCGGCTTTACCGCCGCCGTC
AATATCGACGACGTGCAGGGCTCCACGCTGGCCGCCCGCCATCTTATCAATCTCGGACATCGCCGCATCACCTATATCAG
CACCAATCGCGAGGTGTCGCTGAGCTTCTCCGTGCAAAGCCGATTCGACTCATTCACCGCCTGCTGCCGCAGGGAAGGCA
TCGAGCCGCAGGTCATCGTCTGCAAGGTCGATGACGATGGACGTTATGAAATCAGCGACGTGGTCGACCAACTCATGAGC
CAAGACGAGATGCCCACCGCCATCGCATGCCAGGAGGATGGCATCGCTTTGCCGCTTATGTTCCAGCTGGAGCGCAATGG
ATTCTCCGTGCCCGGCGACGTCTCGCTGGTCGGCTACGACGATAGTTTCTACACCGGCGATATCGGCCTGACCACCATCC
GCCAGGATCCGGCGGAGATGGCCCGCGTGGCAGCCCGCATGACGCTCGACCTCATTGACGAGAAGCCGATTGAGCAACCC
TATATCGTGTTCCCGGCCCAGCTGAAGGTCCGCTCCTCCACCTCCCGCGTTAAGTAG

Upstream 100 bases:

>100_bases
TACAATCGTTGTCATCGCCGCAAATCAATCAATCAGCATGTACACTGGAGTCAGTTGCGCATGTGTATTGCGATTTTCCA
TTCAACGCAAGGGGGCTGCA

Downstream 100 bases:

>100_bases
GCTCACACAACAAAGCCCACCATAGAAAAAGCGTTAGGCAACCGCACAATAACGCTCAATCGCACATTGTGGGAAATGGC
GAAAAATCGCCAATCCATGG

Product: regulatory protein, LacI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MATSIQDVAREAGVSISTVSRSFTRPDLVSAKTRERVLAIADKLNFSLSRSAAALKSGRALRIAVLMSGHIRLWFTASVI
EGLNEVLHTQGYDISVFQISSIEERKEFFEMLPVRRNADAVIVASFDIDNNEIAQLASVGVPIVGINSVEPEARGFTAAV
NIDDVQGSTLAARHLINLGHRRITYISTNREVSLSFSVQSRFDSFTACCRREGIEPQVIVCKVDDDGRYEISDVVDQLMS
QDEMPTAIACQEDGIALPLMFQLERNGFSVPGDVSLVGYDDSFYTGDIGLTTIRQDPAEMARVAARMTLDLIDEKPIEQP
YIVFPAQLKVRSSTSRVK

Sequences:

>Translated_338_residues
MATSIQDVAREAGVSISTVSRSFTRPDLVSAKTRERVLAIADKLNFSLSRSAAALKSGRALRIAVLMSGHIRLWFTASVI
EGLNEVLHTQGYDISVFQISSIEERKEFFEMLPVRRNADAVIVASFDIDNNEIAQLASVGVPIVGINSVEPEARGFTAAV
NIDDVQGSTLAARHLINLGHRRITYISTNREVSLSFSVQSRFDSFTACCRREGIEPQVIVCKVDDDGRYEISDVVDQLMS
QDEMPTAIACQEDGIALPLMFQLERNGFSVPGDVSLVGYDDSFYTGDIGLTTIRQDPAEMARVAARMTLDLIDEKPIEQP
YIVFPAQLKVRSSTSRVK
>Mature_337_residues
ATSIQDVAREAGVSISTVSRSFTRPDLVSAKTRERVLAIADKLNFSLSRSAAALKSGRALRIAVLMSGHIRLWFTASVIE
GLNEVLHTQGYDISVFQISSIEERKEFFEMLPVRRNADAVIVASFDIDNNEIAQLASVGVPIVGINSVEPEARGFTAAVN
IDDVQGSTLAARHLINLGHRRITYISTNREVSLSFSVQSRFDSFTACCRREGIEPQVIVCKVDDDGRYEISDVVDQLMSQ
DEMPTAIACQEDGIALPLMFQLERNGFSVPGDVSLVGYDDSFYTGDIGLTTIRQDPAEMARVAARMTLDLIDEKPIEQPY
IVFPAQLKVRSSTSRVK

Specific function: Involved in the regulation of amylase production [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=342, Percent_Identity=28.3625730994152, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789202, Length=342, Percent_Identity=29.8245614035088, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788474, Length=311, Percent_Identity=29.2604501607717, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787948, Length=342, Percent_Identity=28.3625730994152, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790194, Length=342, Percent_Identity=25.7309941520468, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786540, Length=300, Percent_Identity=29.3333333333333, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787906, Length=350, Percent_Identity=23.7142857142857, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994940, Length=299, Percent_Identity=24.7491638795987, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790689, Length=257, Percent_Identity=27.6264591439689, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787580, Length=337, Percent_Identity=24.3323442136499, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37211; Mature: 37080

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSIQDVAREAGVSISTVSRSFTRPDLVSAKTRERVLAIADKLNFSLSRSAAALKSGRA
CCCCHHHHHHHHCCEEEEHHHCCCCCCHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCE
LRIAVLMSGHIRLWFTASVIEGLNEVLHTQGYDISVFQISSIEERKEFFEMLPVRRNADA
EEEEEEECCCEEEEEEHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCE
VIVASFDIDNNEIAQLASVGVPIVGINSVEPEARGFTAAVNIDDVQGSTLAARHLINLGH
EEEEEEECCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCC
RRITYISTNREVSLSFSVQSRFDSFTACCRREGIEPQVIVCKVDDDGRYEISDVVDQLMS
EEEEEEECCCEEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEHHHHHHHHHC
QDEMPTAIACQEDGIALPLMFQLERNGFSVPGDVSLVGYDDSFYTGDIGLTTIRQDPAEM
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCEEEEEECCCEEECCCEEEEECCCHHHH
ARVAARMTLDLIDEKPIEQPYIVFPAQLKVRSSTSRVK
HHHHHHHHHHHHCCCCCCCCEEEECCEEEEECCCCCCC
>Mature Secondary Structure 
ATSIQDVAREAGVSISTVSRSFTRPDLVSAKTRERVLAIADKLNFSLSRSAAALKSGRA
CCCHHHHHHHHCCEEEEHHHCCCCCCHHCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCE
LRIAVLMSGHIRLWFTASVIEGLNEVLHTQGYDISVFQISSIEERKEFFEMLPVRRNADA
EEEEEEECCCEEEEEEHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCCCCE
VIVASFDIDNNEIAQLASVGVPIVGINSVEPEARGFTAAVNIDDVQGSTLAARHLINLGH
EEEEEEECCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCC
RRITYISTNREVSLSFSVQSRFDSFTACCRREGIEPQVIVCKVDDDGRYEISDVVDQLMS
EEEEEEECCCEEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEHHHHHHHHHC
QDEMPTAIACQEDGIALPLMFQLERNGFSVPGDVSLVGYDDSFYTGDIGLTTIRQDPAEM
CCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCEEEEEECCCEEECCCEEEEECCCHHHH
ARVAARMTLDLIDEKPIEQPYIVFPAQLKVRSSTSRVK
HHHHHHHHHHHHCCCCCCCCEEEECCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7539689 [H]