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The map label for this gene is fmt [H]

Identifier: 213692986

GI number: 213692986

Start: 2357775

End: 2358761

Strand: Direct

Name: fmt [H]

Synonym: Blon_2133

Alternate gene names: 213692986

Gene position: 2357775-2358761 (Clockwise)

Preceding gene: 213692985

Following gene: 213692988

Centisome position: 83.23

GC content: 65.05

Gene sequence:

>987_bases
ATGCTCAAACTCGTATTCGCCGGCACCCCCGACGTGGCGGTGCCCTCGCTCAAGGCCTTTGCCACCGACCCACGCTTCGA
CGTGGTCGGCGTCATCACCCGCCCCGACGCGCCTACCGGTCGCGGCCGCAAACTCACGCCCAGCCCGGTCAAGGCGAAGG
CTCTGGAACTCGGGCTGCCCGTCATCGATCTCAAACCCCGCTCCCCCGAATTCATGGAGGCGTTGAACGACCTGCATGCG
GATATCGCCGCTGTCATCGCCTATGGCAATATCCTGCCTAAGAACGTACTCGACGCAGTACCCATGGGCTGGTACAACCT
GCATTTCTCCAACCTGCCAAAGTGGCGTGGCGCCGCTCCCGCCCAGCGTGCGATTTGGGCAGGCGACCCGACCACCGGCG
CCGACGTGTTCAAGGTCGGCGAGGGCTTGGACGATGGCCCGATCGTCGCTTCCCTGACCATCGAACTGACCGGCCGTGAG
ACCTCCGGCGAACTGCTTGACCGTCTGGCCGAAGAAGGCGCACCCATGTATGTGGATGCTCTGGCCGCCGTCGGCGAAGG
CACCGCCACATTCACCGCGCAGCCGGCCGAATGCCTGGAATATGCGCACAAGATCACGGTGGAGGACGCCCGCATCAGTT
GGACGGACGAGGCCGGAGCCATCGACCGCCAGATTCGCGCCTGCACGCCACATCCTGGGGCATGGACCGAACTGTTTGCG
GAGGGGCCAATCGCCGATAATGACGGGTCAACCGCTAAATCCCTCACCCTGCATATCCTCGCCGCGCAACCGGCCGACCA
GTCCAATCCCAACACTCCGGCCGATCTCAAGCCCGGCGAGCTGAAGGTGGGCAAGAAGAACGTGTGGGTTGGTACCGGCA
GCACGCCGCTGGAACTCATCCAAGTCAAGGCGCAGGGCAAGAAGGCCATGCGCGCCGCCGACTGGGCCCGAGGCGCACGC
CTCTCCCCCGCCGCCTGCGTGCGATAA

Upstream 100 bases:

>100_bases
GCGTACGTTTCCAGGATCCGGTCAAGCTGCGTGAACTGCTCATCGCCAATGGCGTGAACATTCCGGCCGTGCAGTGAGCA
TCGACATTTCAAGGAGGACC

Downstream 100 bases:

>100_bases
CAAGCAATATCGCTCCCGTCATCGGAAGCACAGCAAAAAGCCCGGCATCTTCGCGAGGAAGATACCGGGCTTTTCAGCTA
TACAATCGGCGATCAGTCGA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MLKLVFAGTPDVAVPSLKAFATDPRFDVVGVITRPDAPTGRGRKLTPSPVKAKALELGLPVIDLKPRSPEFMEALNDLHA
DIAAVIAYGNILPKNVLDAVPMGWYNLHFSNLPKWRGAAPAQRAIWAGDPTTGADVFKVGEGLDDGPIVASLTIELTGRE
TSGELLDRLAEEGAPMYVDALAAVGEGTATFTAQPAECLEYAHKITVEDARISWTDEAGAIDRQIRACTPHPGAWTELFA
EGPIADNDGSTAKSLTLHILAAQPADQSNPNTPADLKPGELKVGKKNVWVGTGSTPLELIQVKAQGKKAMRAADWARGAR
LSPAACVR

Sequences:

>Translated_328_residues
MLKLVFAGTPDVAVPSLKAFATDPRFDVVGVITRPDAPTGRGRKLTPSPVKAKALELGLPVIDLKPRSPEFMEALNDLHA
DIAAVIAYGNILPKNVLDAVPMGWYNLHFSNLPKWRGAAPAQRAIWAGDPTTGADVFKVGEGLDDGPIVASLTIELTGRE
TSGELLDRLAEEGAPMYVDALAAVGEGTATFTAQPAECLEYAHKITVEDARISWTDEAGAIDRQIRACTPHPGAWTELFA
EGPIADNDGSTAKSLTLHILAAQPADQSNPNTPADLKPGELKVGKKNVWVGTGSTPLELIQVKAQGKKAMRAADWARGAR
LSPAACVR
>Mature_328_residues
MLKLVFAGTPDVAVPSLKAFATDPRFDVVGVITRPDAPTGRGRKLTPSPVKAKALELGLPVIDLKPRSPEFMEALNDLHA
DIAAVIAYGNILPKNVLDAVPMGWYNLHFSNLPKWRGAAPAQRAIWAGDPTTGADVFKVGEGLDDGPIVASLTIELTGRE
TSGELLDRLAEEGAPMYVDALAAVGEGTATFTAQPAECLEYAHKITVEDARISWTDEAGAIDRQIRACTPHPGAWTELFA
EGPIADNDGSTAKSLTLHILAAQPADQSNPNTPADLKPGELKVGKKNVWVGTGSTPLELIQVKAQGKKAMRAADWARGAR
LSPAACVR

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=233, Percent_Identity=28.755364806867, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI238814322, Length=226, Percent_Identity=30.9734513274336, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI21614513, Length=219, Percent_Identity=31.9634703196347, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1789683, Length=323, Percent_Identity=39.3188854489164, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788589, Length=304, Percent_Identity=24.6710526315789, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550868, Length=322, Percent_Identity=26.7080745341615, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI28571984, Length=243, Percent_Identity=27.1604938271605, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34747; Mature: 34747

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLVFAGTPDVAVPSLKAFATDPRFDVVGVITRPDAPTGRGRKLTPSPVKAKALELGLP
CEEEEEECCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCC
VIDLKPRSPEFMEALNDLHADIAAVIAYGNILPKNVLDAVPMGWYNLHFSNLPKWRGAAP
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCC
AQRAIWAGDPTTGADVFKVGEGLDDGPIVASLTIELTGRETSGELLDRLAEEGAPMYVDA
HHHEEECCCCCCCCHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCEEEHH
LAAVGEGTATFTAQPAECLEYAHKITVEDARISWTDEAGAIDRQIRACTPHPGAWTELFA
HHHHCCCCEEEECCHHHHHHHHHHEEEECCEEEECCCCCCHHHHHEECCCCCCHHHHHHH
EGPIADNDGSTAKSLTLHILAAQPADQSNPNTPADLKPGELKVGKKNVWVGTGSTPLELI
CCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCEEEE
QVKAQGKKAMRAADWARGARLSPAACVR
EEHHCCHHHHHHHHHHCCCCCCHHHHCC
>Mature Secondary Structure
MLKLVFAGTPDVAVPSLKAFATDPRFDVVGVITRPDAPTGRGRKLTPSPVKAKALELGLP
CEEEEEECCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCC
VIDLKPRSPEFMEALNDLHADIAAVIAYGNILPKNVLDAVPMGWYNLHFSNLPKWRGAAP
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCC
AQRAIWAGDPTTGADVFKVGEGLDDGPIVASLTIELTGRETSGELLDRLAEEGAPMYVDA
HHHEEECCCCCCCCHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCEEEHH
LAAVGEGTATFTAQPAECLEYAHKITVEDARISWTDEAGAIDRQIRACTPHPGAWTELFA
HHHHCCCCEEEECCHHHHHHHHHHEEEECCEEEECCCCCCHHHHHEECCCCCCHHHHHHH
EGPIADNDGSTAKSLTLHILAAQPADQSNPNTPADLKPGELKVGKKNVWVGTGSTPLELI
CCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCEEEE
QVKAQGKKAMRAADWARGARLSPAACVR
EEHHCCHHHHHHHHHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA