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The map label for this gene is priA [H]

Identifier: 213692984

GI number: 213692984

Start: 2354676

End: 2356988

Strand: Direct

Name: priA [H]

Synonym: Blon_2131

Alternate gene names: 213692984

Gene position: 2354676-2356988 (Clockwise)

Preceding gene: 213692983

Following gene: 213692985

Centisome position: 83.12

GC content: 63.86

Gene sequence:

>2313_bases
ATGACTACTTCAGGCGCAGAACAGTTGACGCTTGCGGGGCTGGCTCCCCGCAAGCGGCGTAAACGTGCGCCTGCGGAGCA
TACGCCGGCCGAGCAGCATCCCATAGCGCAAGTCGTGCTGGATGTGCAGGCGCTCCACCTCGGTCAGACATTCGATTACT
TCATTGATGAAAAAGACAGCGAGGCCGCACAACCGGGTGTGCTGGTGCGCGTGCGGTTTGGCGGTCAGAGGGTCAGCGGC
GTGATCTGGGCACGCACCGATACCAGCGATACGCCGCGCTCTTCGATTCGCTATATCGAGCGAGTGCTAAGCCCCGATGT
ACTGGTTCCCGCTTCGATGCGTGAGGATATCAGTCTGGTTGCCAAAGCCTATGGCGGCACACGGGCCAATATTCTGCGTT
TCGCCGTGCCACCGCGCGTGGCCAAGGTAGAGACCGAGCAGCGGTTGGCCGCATCGTTCCGTCGGCCGGTCGGCGGCTCA
TTGCCGGATAATACGCAGGGTGGTTTTGCCGGGCGTGGCACCAATCCCGATGGGACCAAACCTGCTGGCTCTACCTTTGC
CGTTGCCAGTACCGTTTCGGAGGGAGCTGCGCAGGGGTATCGTCGTCTGACCGCCAATTACGCCGATGTCAATGTGCTTC
ATGACGCGCTTACCGGTCAACGTTTCCAATCTTTTGTGTTTGATTCGTTGCCCGGTGCACAGGAGTGGCAACGAAACATG
GCATGGATGGTGGCCACTGCGTTGAGTGCCGGTAAGGCTGCCGTAGTTGAATTGCCCACCATGCGCGAGGTCGAAGACCT
GATGCCTATGCTACGCAATTATGGACTCAAACCGTTCGCCCCGGCACCCGCCGGCGGCTGGGTGGGCGACGTCGCCGTGC
TCAATGCGGAGACCATGCCTGCGGCCGACCGGTATCGCACGTATCTGGCCGTGGCGTTGGGCCAGGTGAAGGTGGTGATC
GGCACCCGCGCGGTGATGTATGCGCCGGTTGCAGGCCCGGCACTGTTCGCCATTCTGGAGGATGCCGCCTATCAGAACAT
GGACGGTATGATGCCATATCCACAGGCCCGTGGCGTGATGCGGCTGCGCGCCAAGTCCCATGGCGGCGTGTTCGTGGCCA
TGGCCAATGCGCGCACCCCGCAAAGCCAGTGGGAAAACACCGGCCCGGATACGGTGGAGACCCCGGTGAGCGGCTATTCC
ACCGCGATTCACCCGCTCGCCTCGCCGCTGAAGAATGCGACGCCTTGGGTACGTTGGCTCAATCGCGATGAGCTTGCGCG
TCTTGCCGACCCGTCCATCGGCGCACGTGTGCCGCATACAGCTGTCCGCGTGCTTTCCAAAGCGCTGGAGAGCGGCCCGG
TGCTGTTGTCCATTCCGCAAGACAATGTCAGCGAGACCTTGAGCTGTGCGAAATGCCATCGTCAGGTGCGCTGCGCCAAG
TGTTCCGGACCATTGCAACTGCCGGCCGACCGCCGGGACTCCACACCGCGATGCCGCTGGTGCGGGGCTGCCGCCATCAA
CTGGAAGTGTCCGGGCTGTGGGCATGAACGTATGCGCGTGGTGCGGGTGGGGGCTGCCGGCACTGCGGCGGAGCTGGCCG
GATTGTTCCGTGGCGTGCCAGTAGTTTTGTCCAGCAAGACGCAGGGTCTGGTGCGCGATGTGGCTTGCCAGCCGATGATT
GTCATCGCCACTCCCGGTTTCGAACCGCGCGTGCGTCCGGTCAGTGCCGCGCAGGGAACATCCGGTCATGAGTATCGCGC
AGTGGCCGTGCTCGACGCTTGGACCAGCCTGTATGCGTTGGGCGTGGATGCTCGGTTGGATACGTTGACCGCGTGGATGC
GTGCGGTGTCGTTGTGCGCGCCGCGTTCCCGTGGCGGCCAGGCGTTGATTCTCGGCGAAACCGACCCGGCCATCGCCCAA
TCGCTGATGCTCTGGGATTCGAGGATTCTTGCAGCCAAGGATCTTGAGGAGCGTGTGGAGACCGGTATGCCGCCGGCCGT
GGCCGCCGCCTGCGTGTGGGGCCGCCGTGATGCGGTGATGACGCTGATGCAGCGTATCGGCGCCCTGGGCGGCGATTGGA
CGGCCTGCGGCGAGCTGCCCGGCATGTTGGGCCCGGTGCCGATCGCGCAACCGGACACGGTCGACGCCCGGGAGCTCGAA
GCCACGGCCGACCGGGTGAAGGCAGTAATCCGTGTGCCGCAGAGTCGGCGAGCCGAGCTGGCGGCCCGACTGCACCGGGA
AACCGCTCGTCATGTGGCGTCTCGCGAACCCGGCGAGCTTCGCTTCCGAGTAGATCCCAAAGACCTGATCTAA

Upstream 100 bases:

>100_bases
CGCCGACAAAGCCATCAACGACCGCGACCTAACCCACGTACTCGATTACCTGCGCCGGTAAAATTTTACCGACCTCGGCT
CCGGCACTTATAGTTGAGCC

Downstream 100 bases:

>100_bases
TCGTCTTCAACACGTGAAACGATAATTACAGAACGAACATCGCTATAAGGAGGACACCATGAAGGGCTGGCCTGGAGAGC
CGGATATGGATTACGACGTG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MTTSGAEQLTLAGLAPRKRRKRAPAEHTPAEQHPIAQVVLDVQALHLGQTFDYFIDEKDSEAAQPGVLVRVRFGGQRVSG
VIWARTDTSDTPRSSIRYIERVLSPDVLVPASMREDISLVAKAYGGTRANILRFAVPPRVAKVETEQRLAASFRRPVGGS
LPDNTQGGFAGRGTNPDGTKPAGSTFAVASTVSEGAAQGYRRLTANYADVNVLHDALTGQRFQSFVFDSLPGAQEWQRNM
AWMVATALSAGKAAVVELPTMREVEDLMPMLRNYGLKPFAPAPAGGWVGDVAVLNAETMPAADRYRTYLAVALGQVKVVI
GTRAVMYAPVAGPALFAILEDAAYQNMDGMMPYPQARGVMRLRAKSHGGVFVAMANARTPQSQWENTGPDTVETPVSGYS
TAIHPLASPLKNATPWVRWLNRDELARLADPSIGARVPHTAVRVLSKALESGPVLLSIPQDNVSETLSCAKCHRQVRCAK
CSGPLQLPADRRDSTPRCRWCGAAAINWKCPGCGHERMRVVRVGAAGTAAELAGLFRGVPVVLSSKTQGLVRDVACQPMI
VIATPGFEPRVRPVSAAQGTSGHEYRAVAVLDAWTSLYALGVDARLDTLTAWMRAVSLCAPRSRGGQALILGETDPAIAQ
SLMLWDSRILAAKDLEERVETGMPPAVAAACVWGRRDAVMTLMQRIGALGGDWTACGELPGMLGPVPIAQPDTVDARELE
ATADRVKAVIRVPQSRRAELAARLHRETARHVASREPGELRFRVDPKDLI

Sequences:

>Translated_770_residues
MTTSGAEQLTLAGLAPRKRRKRAPAEHTPAEQHPIAQVVLDVQALHLGQTFDYFIDEKDSEAAQPGVLVRVRFGGQRVSG
VIWARTDTSDTPRSSIRYIERVLSPDVLVPASMREDISLVAKAYGGTRANILRFAVPPRVAKVETEQRLAASFRRPVGGS
LPDNTQGGFAGRGTNPDGTKPAGSTFAVASTVSEGAAQGYRRLTANYADVNVLHDALTGQRFQSFVFDSLPGAQEWQRNM
AWMVATALSAGKAAVVELPTMREVEDLMPMLRNYGLKPFAPAPAGGWVGDVAVLNAETMPAADRYRTYLAVALGQVKVVI
GTRAVMYAPVAGPALFAILEDAAYQNMDGMMPYPQARGVMRLRAKSHGGVFVAMANARTPQSQWENTGPDTVETPVSGYS
TAIHPLASPLKNATPWVRWLNRDELARLADPSIGARVPHTAVRVLSKALESGPVLLSIPQDNVSETLSCAKCHRQVRCAK
CSGPLQLPADRRDSTPRCRWCGAAAINWKCPGCGHERMRVVRVGAAGTAAELAGLFRGVPVVLSSKTQGLVRDVACQPMI
VIATPGFEPRVRPVSAAQGTSGHEYRAVAVLDAWTSLYALGVDARLDTLTAWMRAVSLCAPRSRGGQALILGETDPAIAQ
SLMLWDSRILAAKDLEERVETGMPPAVAAACVWGRRDAVMTLMQRIGALGGDWTACGELPGMLGPVPIAQPDTVDARELE
ATADRVKAVIRVPQSRRAELAARLHRETARHVASREPGELRFRVDPKDLI
>Mature_769_residues
TTSGAEQLTLAGLAPRKRRKRAPAEHTPAEQHPIAQVVLDVQALHLGQTFDYFIDEKDSEAAQPGVLVRVRFGGQRVSGV
IWARTDTSDTPRSSIRYIERVLSPDVLVPASMREDISLVAKAYGGTRANILRFAVPPRVAKVETEQRLAASFRRPVGGSL
PDNTQGGFAGRGTNPDGTKPAGSTFAVASTVSEGAAQGYRRLTANYADVNVLHDALTGQRFQSFVFDSLPGAQEWQRNMA
WMVATALSAGKAAVVELPTMREVEDLMPMLRNYGLKPFAPAPAGGWVGDVAVLNAETMPAADRYRTYLAVALGQVKVVIG
TRAVMYAPVAGPALFAILEDAAYQNMDGMMPYPQARGVMRLRAKSHGGVFVAMANARTPQSQWENTGPDTVETPVSGYST
AIHPLASPLKNATPWVRWLNRDELARLADPSIGARVPHTAVRVLSKALESGPVLLSIPQDNVSETLSCAKCHRQVRCAKC
SGPLQLPADRRDSTPRCRWCGAAAINWKCPGCGHERMRVVRVGAAGTAAELAGLFRGVPVVLSSKTQGLVRDVACQPMIV
IATPGFEPRVRPVSAAQGTSGHEYRAVAVLDAWTSLYALGVDARLDTLTAWMRAVSLCAPRSRGGQALILGETDPAIAQS
LMLWDSRILAAKDLEERVETGMPPAVAAACVWGRRDAVMTLMQRIGALGGDWTACGELPGMLGPVPIAQPDTVDARELEA
TADRVKAVIRVPQSRRAELAARLHRETARHVASREPGELRFRVDPKDLI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 82865; Mature: 82734

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSGAEQLTLAGLAPRKRRKRAPAEHTPAEQHPIAQVVLDVQALHLGQTFDYFIDEKDS
CCCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCC
EAAQPGVLVRVRFGGQRVSGVIWARTDTSDTPRSSIRYIERVLSPDVLVPASMREDISLV
CCCCCCEEEEEEECCCEECEEEEEECCCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHH
AKAYGGTRANILRFAVPPRVAKVETEQRLAASFRRPVGGSLPDNTQGGFAGRGTNPDGTK
HHHCCCCHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAGSTFAVASTVSEGAAQGYRRLTANYADVNVLHDALTGQRFQSFVFDSLPGAQEWQRNM
CCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AWMVATALSAGKAAVVELPTMREVEDLMPMLRNYGLKPFAPAPAGGWVGDVAVLNAETMP
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCC
AADRYRTYLAVALGQVKVVIGTRAVMYAPVAGPALFAILEDAAYQNMDGMMPYPQARGVM
CHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCE
RLRAKSHGGVFVAMANARTPQSQWENTGPDTVETPVSGYSTAIHPLASPLKNATPWVRWL
EEEECCCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHC
NRDELARLADPSIGARVPHTAVRVLSKALESGPVLLSIPQDNVSETLSCAKCHRQVRCAK
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
CSGPLQLPADRRDSTPRCRWCGAAAINWKCPGCGHERMRVVRVGAAGTAAELAGLFRGVP
CCCCCCCCCCCCCCCCCEEECCCCEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCC
VVLSSKTQGLVRDVACQPMIVIATPGFEPRVRPVSAAQGTSGHEYRAVAVLDAWTSLYAL
EEECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHH
GVDARLDTLTAWMRAVSLCAPRSRGGQALILGETDPAIAQSLMLWDSRILAAKDLEERVE
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
TGMPPAVAAACVWGRRDAVMTLMQRIGALGGDWTACGELPGMLGPVPIAQPDTVDARELE
CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHH
ATADRVKAVIRVPQSRRAELAARLHRETARHVASREPGELRFRVDPKDLI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCC
>Mature Secondary Structure 
TTSGAEQLTLAGLAPRKRRKRAPAEHTPAEQHPIAQVVLDVQALHLGQTFDYFIDEKDS
CCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCC
EAAQPGVLVRVRFGGQRVSGVIWARTDTSDTPRSSIRYIERVLSPDVLVPASMREDISLV
CCCCCCEEEEEEECCCEECEEEEEECCCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHH
AKAYGGTRANILRFAVPPRVAKVETEQRLAASFRRPVGGSLPDNTQGGFAGRGTNPDGTK
HHHCCCCHHCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAGSTFAVASTVSEGAAQGYRRLTANYADVNVLHDALTGQRFQSFVFDSLPGAQEWQRNM
CCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AWMVATALSAGKAAVVELPTMREVEDLMPMLRNYGLKPFAPAPAGGWVGDVAVLNAETMP
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCC
AADRYRTYLAVALGQVKVVIGTRAVMYAPVAGPALFAILEDAAYQNMDGMMPYPQARGVM
CHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCE
RLRAKSHGGVFVAMANARTPQSQWENTGPDTVETPVSGYSTAIHPLASPLKNATPWVRWL
EEEECCCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHC
NRDELARLADPSIGARVPHTAVRVLSKALESGPVLLSIPQDNVSETLSCAKCHRQVRCAK
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
CSGPLQLPADRRDSTPRCRWCGAAAINWKCPGCGHERMRVVRVGAAGTAAELAGLFRGVP
CCCCCCCCCCCCCCCCCEEECCCCEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCC
VVLSSKTQGLVRDVACQPMIVIATPGFEPRVRPVSAAQGTSGHEYRAVAVLDAWTSLYAL
EEECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHH
GVDARLDTLTAWMRAVSLCAPRSRGGQALILGETDPAIAQSLMLWDSRILAAKDLEERVE
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
TGMPPAVAAACVWGRRDAVMTLMQRIGALGGDWTACGELPGMLGPVPIAQPDTVDARELE
CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHH
ATADRVKAVIRVPQSRRAELAARLHRETARHVASREPGELRFRVDPKDLI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]