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The map label for this gene is dop [H]

Identifier: 213692980

GI number: 213692980

Start: 2349850

End: 2351517

Strand: Reverse

Name: dop [H]

Synonym: Blon_2127

Alternate gene names: 213692980

Gene position: 2351517-2349850 (Counterclockwise)

Preceding gene: 213692981

Following gene: 213692979

Centisome position: 83.01

GC content: 64.39

Gene sequence:

>1668_bases
ATGACCGTCAAACGAGTGATGGGCACCGAAACCGAATACGCGGTATCCCTGAACACCCCGGATCGCTACAATCCGGTGCA
ACTGTCCTTCGACGTGGTGAACGGCGCCGCGGACAGCCACAGCAAATCCATCCGCTGGGACTACCGGCAGGAGGACCCGG
TCAACGACGCGCGCGGCACTCGCCTCGAACGCGCCGCCGCCCGCCCGGACATGCTCACCGACGCCCCACAACTCAACATC
ACCAACGTGATCGCCCCCAATGGCGGCCGCGTCTACGTGGACCACGCCCACCCCGAATACTCGGCACCGGAAACCACCGA
CCCGTTCGAAGCGGTCCGCTATGACCACGCCGGGGACCTCATCATGCAGGCCGCCACCGAACGCGCCCGCACACAGACCG
GCACCCCCGTCGCCCTGCATCGCAACAATGTGGACGGCAAGGGCTCCTGTTGGGGCACGCACGAGAACTATATGATGGCC
CGCTCAGTGCCATTCGATTTGGTCACGCGCCTGATGACCCTCCACTTCGTCACCCGCCAGATCTACGCCGGCTCCGGCCG
CGTGGGAATCGGCGAGAACAGCGAGATCCCGGGCTACCAGCTCAGCCAGCGCGCCGACTACATCCATGCCAAAGTCGGTC
TGCAAACCACCTTCGAACGCCCGATCATCAACACGCGCGACGAATCCCACTCCACCGACGCATACCGCCGCCTGCACGTC
ATCGTTGGCGACGCTAACCGCATGGAGGTACCGCAGGCCCTGAAACTCGGCACCACGAGCATGCTGCTGTGGCTGCTCGA
ACACGCGGACGAAGCCGGATTCGATCTGAACGTCTTCCTCGACGAGCTCGAACTGGCCGACCCGGTCGAAGCCATGCACA
CCGTCTCCCATGACCTGACGCTGGGCGCAAGCCTGCCGCTGGCCAACGGCGGCGAAACCAACGCATGGCTTATCCAGCTC
AAACTGCGCCAAGCCGTCTACCAGGTCGCCGCACTGGTCGAAGGCACCGACACCGCCGGCGAGCCCGCCTGGCCGGACAA
ATCCACCACATCCATCATGGCGATGTGGCAGCAGGCGCTCATCGACTGCGCCTCCATCCGCCATGCCGCCGACGATGACG
AACGCCTGGCCATGACCGGCGAGGCCAGCCGCATCGAATGGCTGCTCAAATGGCAGCTGCTCGAAAAACTGCGCCGCAAA
ATCACCGCGGCAGCTGTGCCCAGCGTGGCTAACGGATGGAATGATCCGCGACTGAAGGTCATTGAACTGAAATGGGCGGC
GCTCGACCCCGCGGACAGCATCTTCACCAAACTCGAACCCCGCACCGAACGGGTGGTGAGCGCCGCCGACATCGCACGCG
CCGCCACCGAACCGCCGGCAGACACCCGCGCCTGGCTCAGGGCGAAACTCGTTGCACGATTCGGCGATGAGGTGGCCGCC
GCCAGCTGGTCCCGACTCACTGTTCGCGACCCGCGCGCCGCAGACGAGGGCGAGGCTGTCGAATTCTACGGCAATGCCGG
CCATATGGCCGCTACCGACCATCACCTGTTCTCCCTCGACATCTCCGATCCGCTCGCCTTCACCAAGGCGCACTGCGAAA
CCGAACTCAATTTAGCCGAACACGCCATCGATCTGCTGAAGTCGCTGGCCATCAACGCCGAACGATAG

Upstream 100 bases:

>100_bases
CATGGCCGACGTGGACCCCGAACAATGGTCGCGCATCAACGGCATGGACCCGATCCGCCGCATCCGCACCGCCGAATAGC
TCAAGCTCAAGGAGCGCTAC

Downstream 100 bases:

>100_bases
ACTTGATAGCTATGGAAGAAACACGCGAAGAGACCCCACAGGACCTGCGCAACCTTGCGCTCAAAGTCGCCGGCCTTCTC
GAAGAAGCCGGCCAGCATGC

Product: hypothetical protein

Products: NA

Alternate protein names: Deamidase of protein Pup [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MTVKRVMGTETEYAVSLNTPDRYNPVQLSFDVVNGAADSHSKSIRWDYRQEDPVNDARGTRLERAAARPDMLTDAPQLNI
TNVIAPNGGRVYVDHAHPEYSAPETTDPFEAVRYDHAGDLIMQAATERARTQTGTPVALHRNNVDGKGSCWGTHENYMMA
RSVPFDLVTRLMTLHFVTRQIYAGSGRVGIGENSEIPGYQLSQRADYIHAKVGLQTTFERPIINTRDESHSTDAYRRLHV
IVGDANRMEVPQALKLGTTSMLLWLLEHADEAGFDLNVFLDELELADPVEAMHTVSHDLTLGASLPLANGGETNAWLIQL
KLRQAVYQVAALVEGTDTAGEPAWPDKSTTSIMAMWQQALIDCASIRHAADDDERLAMTGEASRIEWLLKWQLLEKLRRK
ITAAAVPSVANGWNDPRLKVIELKWAALDPADSIFTKLEPRTERVVSAADIARAATEPPADTRAWLRAKLVARFGDEVAA
ASWSRLTVRDPRAADEGEAVEFYGNAGHMAATDHHLFSLDISDPLAFTKAHCETELNLAEHAIDLLKSLAINAER

Sequences:

>Translated_555_residues
MTVKRVMGTETEYAVSLNTPDRYNPVQLSFDVVNGAADSHSKSIRWDYRQEDPVNDARGTRLERAAARPDMLTDAPQLNI
TNVIAPNGGRVYVDHAHPEYSAPETTDPFEAVRYDHAGDLIMQAATERARTQTGTPVALHRNNVDGKGSCWGTHENYMMA
RSVPFDLVTRLMTLHFVTRQIYAGSGRVGIGENSEIPGYQLSQRADYIHAKVGLQTTFERPIINTRDESHSTDAYRRLHV
IVGDANRMEVPQALKLGTTSMLLWLLEHADEAGFDLNVFLDELELADPVEAMHTVSHDLTLGASLPLANGGETNAWLIQL
KLRQAVYQVAALVEGTDTAGEPAWPDKSTTSIMAMWQQALIDCASIRHAADDDERLAMTGEASRIEWLLKWQLLEKLRRK
ITAAAVPSVANGWNDPRLKVIELKWAALDPADSIFTKLEPRTERVVSAADIARAATEPPADTRAWLRAKLVARFGDEVAA
ASWSRLTVRDPRAADEGEAVEFYGNAGHMAATDHHLFSLDISDPLAFTKAHCETELNLAEHAIDLLKSLAINAER
>Mature_554_residues
TVKRVMGTETEYAVSLNTPDRYNPVQLSFDVVNGAADSHSKSIRWDYRQEDPVNDARGTRLERAAARPDMLTDAPQLNIT
NVIAPNGGRVYVDHAHPEYSAPETTDPFEAVRYDHAGDLIMQAATERARTQTGTPVALHRNNVDGKGSCWGTHENYMMAR
SVPFDLVTRLMTLHFVTRQIYAGSGRVGIGENSEIPGYQLSQRADYIHAKVGLQTTFERPIINTRDESHSTDAYRRLHVI
VGDANRMEVPQALKLGTTSMLLWLLEHADEAGFDLNVFLDELELADPVEAMHTVSHDLTLGASLPLANGGETNAWLIQLK
LRQAVYQVAALVEGTDTAGEPAWPDKSTTSIMAMWQQALIDCASIRHAADDDERLAMTGEASRIEWLLKWQLLEKLRRKI
TAAAVPSVANGWNDPRLKVIELKWAALDPADSIFTKLEPRTERVVSAADIARAATEPPADTRAWLRAKLVARFGDEVAAA
SWSRLTVRDPRAADEGEAVEFYGNAGHMAATDHHLFSLDISDPLAFTKAHCETELNLAEHAIDLLKSLAINAER

Specific function: Specifically catalyzes the deamidation of the C-terminal glutamine of the prokaryotic ubiquitin-like protein Pup to glutamate, thereby rendering Pup competent for conjugation. Also displays depupylase (DPUP) activity, removing conjugated Pup from target p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Pup ligase/Pup deamidase family. Pup deamidase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022366
- InterPro:   IPR004347 [H]

Pfam domain/function: PF03136 DUF245 [H]

EC number: NA

Molecular weight: Translated: 61410; Mature: 61278

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKRVMGTETEYAVSLNTPDRYNPVQLSFDVVNGAADSHSKSIRWDYRQEDPVNDARGT
CCCHHHCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCC
RLERAAARPDMLTDAPQLNITNVIAPNGGRVYVDHAHPEYSAPETTDPFEAVRYDHAGDL
HHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCHHH
IMQAATERARTQTGTPVALHRNNVDGKGSCWGTHENYMMARSVPFDLVTRLMTLHFVTRQ
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
IYAGSGRVGIGENSEIPGYQLSQRADYIHAKVGLQTTFERPIINTRDESHSTDAYRRLHV
HHCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEE
IVGDANRMEVPQALKLGTTSMLLWLLEHADEAGFDLNVFLDELELADPVEAMHTVSHDLT
EEECCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCHHHHHHHHHCCEE
LGASLPLANGGETNAWLIQLKLRQAVYQVAALVEGTDTAGEPAWPDKSTTSIMAMWQQAL
ECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
IDCASIRHAADDDERLAMTGEASRIEWLLKWQLLEKLRRKITAAAVPSVANGWNDPRLKV
HHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEE
IELKWAALDPADSIFTKLEPRTERVVSAADIARAATEPPADTRAWLRAKLVARFGDEVAA
EEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHH
ASWSRLTVRDPRAADEGEAVEFYGNAGHMAATDHHLFSLDISDPLAFTKAHCETELNLAE
CCCCEEEEECCCCCCCCCEEEEECCCCCCEECCCEEEEEECCCCHHHHHHHHHHHHHHHH
HAIDLLKSLAINAER
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVKRVMGTETEYAVSLNTPDRYNPVQLSFDVVNGAADSHSKSIRWDYRQEDPVNDARGT
CCHHHCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCC
RLERAAARPDMLTDAPQLNITNVIAPNGGRVYVDHAHPEYSAPETTDPFEAVRYDHAGDL
HHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCHHH
IMQAATERARTQTGTPVALHRNNVDGKGSCWGTHENYMMARSVPFDLVTRLMTLHFVTRQ
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
IYAGSGRVGIGENSEIPGYQLSQRADYIHAKVGLQTTFERPIINTRDESHSTDAYRRLHV
HHCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEE
IVGDANRMEVPQALKLGTTSMLLWLLEHADEAGFDLNVFLDELELADPVEAMHTVSHDLT
EEECCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCHHHHHHHHHCCEE
LGASLPLANGGETNAWLIQLKLRQAVYQVAALVEGTDTAGEPAWPDKSTTSIMAMWQQAL
ECCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
IDCASIRHAADDDERLAMTGEASRIEWLLKWQLLEKLRRKITAAAVPSVANGWNDPRLKV
HHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEE
IELKWAALDPADSIFTKLEPRTERVVSAADIARAATEPPADTRAWLRAKLVARFGDEVAA
EEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHH
ASWSRLTVRDPRAADEGEAVEFYGNAGHMAATDHHLFSLDISDPLAFTKAHCETELNLAE
CCCCEEEEECCCCCCCCCEEEEECCCCCCEECCCEEEEEECCCCHHHHHHHHHHHHHHHH
HAIDLLKSLAINAER
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]