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The map label for this gene is trmL [H]

Identifier: 213692908

GI number: 213692908

Start: 2263034

End: 2263543

Strand: Direct

Name: trmL [H]

Synonym: Blon_2052

Alternate gene names: 213692908

Gene position: 2263034-2263543 (Clockwise)

Preceding gene: 213692903

Following gene: 213692921

Centisome position: 79.89

GC content: 61.76

Gene sequence:

>510_bases
ATGCTCTCGGCTGAAAAGGCCGCCACCACCGAAACCGTAACACCGCACCTGTGCTACTACTCACCGCGTATTCCCGGCAA
CACCGGCTCGGCCATCCGCCTGTGCGCGGTGACCGGCACCATCCTGCATTTGGTGGAGCCGTTGGGTTTCAACCTGCGCG
ACACCAAGCTGCGCCGAGCAGGCCTCGACTACCACGACATGGCCCACGTGGTGCTGCATCCGAATTTCGACAACCTGGTG
GAGTCCATGCCCAATTCGCGCATCATCGCGTTCACCGCACATGCCACCAAGCTGTACACCGAAGTGGAATACAAGCCGAC
CGACATCCTGCTGTTCGGCCCCGAACCCGGCGACATCCCCGACCCGATGGACATCATGGAAGGCCCGCATGTGGCCGAAC
AGGTGCGACTGCCCATGCGCCCGTCCTTGCGTTCCCTGAACCTCACCAATTGCGCGTCCATCGCCATCTACGAAGCCTGG
CGCCAACTGAATTTCAAGGGCGGAGCGTGA

Upstream 100 bases:

>100_bases
AGTCGAGACGATGTTCGAGTACGGGTACCGTAAATCGAATTACGGACCGGACGAACTCGTAACCGACGCGCATGGCAACC
CCATCTCCGTGGTGGACGCG

Downstream 100 bases:

>100_bases
GGAAAAGGCCCGGTGGGCCTTCGAGTAGCGACGACGAACGAACACCGACAGGCGTGAGAGAGGAAACCCTGAGCCTCGCG
CAGCGAGACCACAATTGCAG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MLSAEKAATTETVTPHLCYYSPRIPGNTGSAIRLCAVTGTILHLVEPLGFNLRDTKLRRAGLDYHDMAHVVLHPNFDNLV
ESMPNSRIIAFTAHATKLYTEVEYKPTDILLFGPEPGDIPDPMDIMEGPHVAEQVRLPMRPSLRSLNLTNCASIAIYEAW
RQLNFKGGA

Sequences:

>Translated_169_residues
MLSAEKAATTETVTPHLCYYSPRIPGNTGSAIRLCAVTGTILHLVEPLGFNLRDTKLRRAGLDYHDMAHVVLHPNFDNLV
ESMPNSRIIAFTAHATKLYTEVEYKPTDILLFGPEPGDIPDPMDIMEGPHVAEQVRLPMRPSLRSLNLTNCASIAIYEAW
RQLNFKGGA
>Mature_169_residues
MLSAEKAATTETVTPHLCYYSPRIPGNTGSAIRLCAVTGTILHLVEPLGFNLRDTKLRRAGLDYHDMAHVVLHPNFDNLV
ESMPNSRIIAFTAHATKLYTEVEYKPTDILLFGPEPGDIPDPMDIMEGPHVAEQVRLPMRPSLRSLNLTNCASIAIYEAW
RQLNFKGGA

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=42.483660130719, Blast_Score=138, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18741; Mature: 18741

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAEKAATTETVTPHLCYYSPRIPGNTGSAIRLCAVTGTILHLVEPLGFNLRDTKLRRA
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHC
GLDYHDMAHVVLHPNFDNLVESMPNSRIIAFTAHATKLYTEVEYKPTDILLFGPEPGDIP
CCCHHHEEEEEECCCHHHHHHHCCCCEEEEEEECCEEEEEEEEECCCEEEEECCCCCCCC
DPMDIMEGPHVAEQVRLPMRPSLRSLNLTNCASIAIYEAWRQLNFKGGA
CCHHHHCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLSAEKAATTETVTPHLCYYSPRIPGNTGSAIRLCAVTGTILHLVEPLGFNLRDTKLRRA
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHC
GLDYHDMAHVVLHPNFDNLVESMPNSRIIAFTAHATKLYTEVEYKPTDILLFGPEPGDIP
CCCHHHEEEEEECCCHHHHHHHCCCCEEEEEEECCEEEEEEEEECCCEEEEECCCCCCCC
DPMDIMEGPHVAEQVRLPMRPSLRSLNLTNCASIAIYEAWRQLNFKGGA
CCHHHHCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA