Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 213692889

GI number: 213692889

Start: 2219783

End: 2220436

Strand: Reverse

Name: def [H]

Synonym: Blon_2030

Alternate gene names: 213692889

Gene position: 2220436-2219783 (Counterclockwise)

Preceding gene: 213692890

Following gene: 213692888

Centisome position: 78.38

GC content: 60.09

Gene sequence:

>654_bases
ATGTTTGGAAAGAACGCCAAAGTCGACCTCGAACTCAACCGTGACGTCGAACAACTCATCAAAACCGGCGGCAAGGAGAA
GATTCTGCCCATCGTGCAGGCCGGCGAACCGGTGTTGCGCCAACGGACAGTGGCCTACAATGGCCAGCTTTCCAAGCGTA
CGCTCGCCAAACTCATCGATACGATGCACACCACCATGCTGGAGGCACCCGGCGTGGGGCTCGCCGCCACCCAGATCGGC
TTGGGGCTCGCGCTCGCCGTGGTCGAAGACCACGTGCGCGACGACGAGGATGATCCGCGCGAAATCGCGGAATTCCCCTT
CCACGTGATCATCAATCCGAGCTACAAACCCACCAGCGACAAAACCGCCTCGTTCTACGAAGGCTGCCTGAGCTTCGACG
GATACCAAGCCGTGCGCAAGCGTTGGCTCGACATCACCGCCGAATGGGATGATGAAGACGGCAAACACCACAGCGAGCCG
CTGCACGGCTGGCCCGCACGCATCTTCCAGCACGAAACCGATCATTTGAGCGGCGAGCTGTATATCGATCGCGCCGAAAT
CCGTTCGCTGACCACCAACGAGAACCTCGAGGACTATTGGTGCGACGACCCGGTGCCCACCGAGGCCGCTGAAGAGCTCG
GTTTCGCACTATGA

Upstream 100 bases:

>100_bases
GAGGCCGCCACCCAGGCCTACGCCGACGAAGTCTGCACCCGCCTCGCCAAGATCGTCGCCGAAGAACTCGCGCTGTAGCA
CCCTTCCCTAAGGAAAAACA

Downstream 100 bases:

>100_bases
CCGACCGCGCATGGTTCGACGCCGCCAAGGGAGACATTCCCCAATGGGCGGCGGCTATGCCCAAGGACGCCGATGGCACC
ACCATCGACTGGGACTGGGT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MFGKNAKVDLELNRDVEQLIKTGGKEKILPIVQAGEPVLRQRTVAYNGQLSKRTLAKLIDTMHTTMLEAPGVGLAATQIG
LGLALAVVEDHVRDDEDDPREIAEFPFHVIINPSYKPTSDKTASFYEGCLSFDGYQAVRKRWLDITAEWDDEDGKHHSEP
LHGWPARIFQHETDHLSGELYIDRAEIRSLTTNENLEDYWCDDPVPTEAAEELGFAL

Sequences:

>Translated_217_residues
MFGKNAKVDLELNRDVEQLIKTGGKEKILPIVQAGEPVLRQRTVAYNGQLSKRTLAKLIDTMHTTMLEAPGVGLAATQIG
LGLALAVVEDHVRDDEDDPREIAEFPFHVIINPSYKPTSDKTASFYEGCLSFDGYQAVRKRWLDITAEWDDEDGKHHSEP
LHGWPARIFQHETDHLSGELYIDRAEIRSLTTNENLEDYWCDDPVPTEAAEELGFAL
>Mature_217_residues
MFGKNAKVDLELNRDVEQLIKTGGKEKILPIVQAGEPVLRQRTVAYNGQLSKRTLAKLIDTMHTTMLEAPGVGLAATQIG
LGLALAVVEDHVRDDEDDPREIAEFPFHVIINPSYKPTSDKTASFYEGCLSFDGYQAVRKRWLDITAEWDDEDGKHHSEP
LHGWPARIFQHETDHLSGELYIDRAEIRSLTTNENLEDYWCDDPVPTEAAEELGFAL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=169, Percent_Identity=33.1360946745562, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1789682, Length=144, Percent_Identity=31.9444444444444, Blast_Score=64, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24645728, Length=167, Percent_Identity=31.7365269461078, Blast_Score=90, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24645726, Length=160, Percent_Identity=32.5, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 24430; Mature: 24430

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGKNAKVDLELNRDVEQLIKTGGKEKILPIVQAGEPVLRQRTVAYNGQLSKRTLAKLID
CCCCCCEEEEEECCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHH
TMHTTMLEAPGVGLAATQIGLGLALAVVEDHVRDDEDDPREIAEFPFHVIINPSYKPTSD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCCCCCCC
KTASFYEGCLSFDGYQAVRKRWLDITAEWDDEDGKHHSEPLHGWPARIFQHETDHLSGEL
CHHHHHHHHHCCCHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEE
YIDRAEIRSLTTNENLEDYWCDDPVPTEAAEELGFAL
EEEHHHHHHHCCCCCCCHHCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MFGKNAKVDLELNRDVEQLIKTGGKEKILPIVQAGEPVLRQRTVAYNGQLSKRTLAKLID
CCCCCCEEEEEECCCHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHH
TMHTTMLEAPGVGLAATQIGLGLALAVVEDHVRDDEDDPREIAEFPFHVIINPSYKPTSD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCCCCCCC
KTASFYEGCLSFDGYQAVRKRWLDITAEWDDEDGKHHSEPLHGWPARIFQHETDHLSGEL
CHHHHHHHHHCCCHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEE
YIDRAEIRSLTTNENLEDYWCDDPVPTEAAEELGFAL
EEEHHHHHHHCCCCCCCHHCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]