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The map label for this gene is nadE [H]

Identifier: 213692585

GI number: 213692585

Start: 1880244

End: 1881941

Strand: Reverse

Name: nadE [H]

Synonym: Blon_1717

Alternate gene names: 213692585

Gene position: 1881941-1880244 (Counterclockwise)

Preceding gene: 213692586

Following gene: 213692584

Centisome position: 66.44

GC content: 63.6

Gene sequence:

>1698_bases
ATGACACAGCTTCGCTTCGCACTCGCCCAGATCGACACCTGCGTCGGCGCGCTTGACGTCAACGCCGCCAAGGTGCTCGA
CTACTCCCGTAAAGCCGCCGCAGGAAACGCCCAGGTGGTAGTCTTCTCGGAAATGACCCTCACCGGATACCCCATCGAGG
ATCTGGCCCTGCGCCGCACCTTCCGCCAAGCCGCATGGGACAAGGCCAACGAACTCGCCACCCAGCTCAACGACGACGGC
CTCGGCGATCTGTTCGTGGTGGTCGGCACCGTGGGCACCGACCGCAAGACCTCCAAGCCGCGCAACCGACTGGTGGTCTT
GCATCAGGGCGTGGTCTGGGCCGGCTACGACAAGCACTTCCTGCCCAACTACGGCGTGTTCGATGAATTCCGCATCTTCT
CCGCCGGCGACCGCTCCGTGACCCTCGACGTGGACGGCGCCACCATTGGCGTGGCCATCTGCGAGGACATCTGGCAGGAC
GGCGGCCCGGTGGCCGACCTGGCCACCAAGAACATCGACCTGCTGCTCACCATCAACGGATCGCCGTATGAGGAAGGCAA
GACCAACACCCGTTTTGAGCTCGCCCAGCGTCGTGCCGCCGAGGTTAACGCCCCGGTCATCTACCTGAATCAGGTCGGCG
GTCAAGACGACCTCGTCTTCGACGGTGGCAGTTTCGTGGTCGACGCCGACGGCACGCTTATCGAACGCAGCCCGATGTTC
ATGGAAAACCTGACGTTCTGGGACTTCGATTCCGCCGCCGAACATCAGGCCAAGGCCGAGATCGTTCCGGAACTCGACCC
GGACGAAGAGGTATACACCGCCTGCGTGCTCGGCCTCAAGGACTACATGGCCAAGAACCACTTCACCGGCGTGACGCTGG
GCCTCTCAGGAGGTATCGACTCCGCACTGGTAGCCGCCATGGCGGCCGACGCATGCGGCGGCGAGAACGTCTGGGGTATC
TCCATGCCCTCCATGTACTCCTCCGATGGTTCCAAAGACGACGCCGCTGACCTGGCCTCCAACATTGGCGCCCATTACGA
GGTTCAGCCCATTGAGCCATTGTTCAACGCCTACCAACAGCAACTTGATCTCCATGGTGTCTCCGCCGAGAACCTGCAGG
CCCGCATCCGCGGTGTGATCGTGATGGCCAGTTCCAATTCGCGCGGTCTGCTCGCCGTGGCCACCGGCAACAAGTCAGAG
CTCGCCTGCGGCTACTCGACCATCTACGGCGATGCGGTAGGCGGTTACGCACCCATCAAGGACCTGCTCAAGACCCGCGT
CTGGGAGATCAGCCGTTGGCGTAACAAGGCCGCGGCCGCGGGCGTCGGCATCGGTGGGCTCAAGATCGTGGGCAACGAGG
ACGGCAACACCGGCGTCCCGCTCAAGGACGGCGTGATGATTCCGGTCTCATCCATCGAGAAGGCTCCCTCGGCCGAGTTG
CGCCCCGGGCAGAAGGACTCCGACTCCCTGCCCGAATACGCGCTGCTCGACAAGGTGCTCGCCGCATACATCGAGCACGC
GCACGGCCGTGCCGACCTGTTAGCCGACGGCTTTGACCAGGCCACCGTAGACACCGTGATGCGATTGGTGGACCGCGCTG
AATGGAAGCGCCGCCAATACCCGCTGGGTCCCAAGGTCACGGCCCTCGCGTTCGGACGTGACCGCCGACTGCCCGTCACC
AACGCATTCAGGGAATAA

Upstream 100 bases:

>100_bases
CGCATTGCAGTGACCTAATGAACGCAAAAACGACCTAATGAATGACGCCAAGCCGGAAGAACAGGCAATCCGACGCCATA
CGCGGCTACACTAAGAAGCT

Downstream 100 bases:

>100_bases
ACATATATTGCTCCCGCCAGCGGGAGCAAGTAATACCGTTGAGGAGGTACCAATGACAGACGATAACAAGAAGCAGTGGT
ATTTCAACACCGTCAAGAAG

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MTQLRFALAQIDTCVGALDVNAAKVLDYSRKAAAGNAQVVVFSEMTLTGYPIEDLALRRTFRQAAWDKANELATQLNDDG
LGDLFVVVGTVGTDRKTSKPRNRLVVLHQGVVWAGYDKHFLPNYGVFDEFRIFSAGDRSVTLDVDGATIGVAICEDIWQD
GGPVADLATKNIDLLLTINGSPYEEGKTNTRFELAQRRAAEVNAPVIYLNQVGGQDDLVFDGGSFVVDADGTLIERSPMF
MENLTFWDFDSAAEHQAKAEIVPELDPDEEVYTACVLGLKDYMAKNHFTGVTLGLSGGIDSALVAAMAADACGGENVWGI
SMPSMYSSDGSKDDAADLASNIGAHYEVQPIEPLFNAYQQQLDLHGVSAENLQARIRGVIVMASSNSRGLLAVATGNKSE
LACGYSTIYGDAVGGYAPIKDLLKTRVWEISRWRNKAAAAGVGIGGLKIVGNEDGNTGVPLKDGVMIPVSSIEKAPSAEL
RPGQKDSDSLPEYALLDKVLAAYIEHAHGRADLLADGFDQATVDTVMRLVDRAEWKRRQYPLGPKVTALAFGRDRRLPVT
NAFRE

Sequences:

>Translated_565_residues
MTQLRFALAQIDTCVGALDVNAAKVLDYSRKAAAGNAQVVVFSEMTLTGYPIEDLALRRTFRQAAWDKANELATQLNDDG
LGDLFVVVGTVGTDRKTSKPRNRLVVLHQGVVWAGYDKHFLPNYGVFDEFRIFSAGDRSVTLDVDGATIGVAICEDIWQD
GGPVADLATKNIDLLLTINGSPYEEGKTNTRFELAQRRAAEVNAPVIYLNQVGGQDDLVFDGGSFVVDADGTLIERSPMF
MENLTFWDFDSAAEHQAKAEIVPELDPDEEVYTACVLGLKDYMAKNHFTGVTLGLSGGIDSALVAAMAADACGGENVWGI
SMPSMYSSDGSKDDAADLASNIGAHYEVQPIEPLFNAYQQQLDLHGVSAENLQARIRGVIVMASSNSRGLLAVATGNKSE
LACGYSTIYGDAVGGYAPIKDLLKTRVWEISRWRNKAAAAGVGIGGLKIVGNEDGNTGVPLKDGVMIPVSSIEKAPSAEL
RPGQKDSDSLPEYALLDKVLAAYIEHAHGRADLLADGFDQATVDTVMRLVDRAEWKRRQYPLGPKVTALAFGRDRRLPVT
NAFRE
>Mature_564_residues
TQLRFALAQIDTCVGALDVNAAKVLDYSRKAAAGNAQVVVFSEMTLTGYPIEDLALRRTFRQAAWDKANELATQLNDDGL
GDLFVVVGTVGTDRKTSKPRNRLVVLHQGVVWAGYDKHFLPNYGVFDEFRIFSAGDRSVTLDVDGATIGVAICEDIWQDG
GPVADLATKNIDLLLTINGSPYEEGKTNTRFELAQRRAAEVNAPVIYLNQVGGQDDLVFDGGSFVVDADGTLIERSPMFM
ENLTFWDFDSAAEHQAKAEIVPELDPDEEVYTACVLGLKDYMAKNHFTGVTLGLSGGIDSALVAAMAADACGGENVWGIS
MPSMYSSDGSKDDAADLASNIGAHYEVQPIEPLFNAYQQQLDLHGVSAENLQARIRGVIVMASSNSRGLLAVATGNKSEL
ACGYSTIYGDAVGGYAPIKDLLKTRVWEISRWRNKAAAAGVGIGGLKIVGNEDGNTGVPLKDGVMIPVSSIEKAPSAELR
PGQKDSDSLPEYALLDKVLAAYIEHAHGRADLLADGFDQATVDTVMRLVDRAEWKRRQYPLGPKVTALAFGRDRRLPVTN
AFRE

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60961; Mature: 60830

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLRFALAQIDTCVGALDVNAAKVLDYSRKAAAGNAQVVVFSEMTLTGYPIEDLALRRT
CCHHHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHH
FRQAAWDKANELATQLNDDGLGDLFVVVGTVGTDRKTSKPRNRLVVLHQGVVWAGYDKHF
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCEEEECCCCCC
LPNYGVFDEFRIFSAGDRSVTLDVDGATIGVAICEDIWQDGGPVADLATKNIDLLLTING
CCCCCCCCCEEEEECCCCEEEEEECCCEEHHHHHHHHHHCCCCEEHHCCCCEEEEEEECC
SPYEEGKTNTRFELAQRRAAEVNAPVIYLNQVGGQDDLVFDGGSFVVDADGTLIERSPMF
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCEEEECCCCEEECCCCH
MENLTFWDFDSAAEHQAKAEIVPELDPDEEVYTACVLGLKDYMAKNHFTGVTLGLSGGID
HHCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
SALVAAMAADACGGENVWGISMPSMYSSDGSKDDAADLASNIGAHYEVQPIEPLFNAYQQ
HHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHH
QLDLHGVSAENLQARIRGVIVMASSNSRGLLAVATGNKSELACGYSTIYGDAVGGYAPIK
HHHHCCCCHHHHHEEEEEEEEEECCCCCCEEEEECCCCCCEEECHHHHCCCCCCCHHHHH
DLLKTRVWEISRWRNKAAAAGVGIGGLKIVGNEDGNTGVPLKDGVMIPVSSIEKAPSAEL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHCCCCCCCC
RPGQKDSDSLPEYALLDKVLAAYIEHAHGRADLLADGFDQATVDTVMRLVDRAEWKRRQY
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
PLGPKVTALAFGRDRRLPVTNAFRE
CCCCCEEEEEECCCCCCCCHHHCCC
>Mature Secondary Structure 
TQLRFALAQIDTCVGALDVNAAKVLDYSRKAAAGNAQVVVFSEMTLTGYPIEDLALRRT
CHHHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHH
FRQAAWDKANELATQLNDDGLGDLFVVVGTVGTDRKTSKPRNRLVVLHQGVVWAGYDKHF
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCEEEECCCCCC
LPNYGVFDEFRIFSAGDRSVTLDVDGATIGVAICEDIWQDGGPVADLATKNIDLLLTING
CCCCCCCCCEEEEECCCCEEEEEECCCEEHHHHHHHHHHCCCCEEHHCCCCEEEEEEECC
SPYEEGKTNTRFELAQRRAAEVNAPVIYLNQVGGQDDLVFDGGSFVVDADGTLIERSPMF
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCEEEECCCCEEECCCCH
MENLTFWDFDSAAEHQAKAEIVPELDPDEEVYTACVLGLKDYMAKNHFTGVTLGLSGGID
HHCCEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCH
SALVAAMAADACGGENVWGISMPSMYSSDGSKDDAADLASNIGAHYEVQPIEPLFNAYQQ
HHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHH
QLDLHGVSAENLQARIRGVIVMASSNSRGLLAVATGNKSELACGYSTIYGDAVGGYAPIK
HHHHCCCCHHHHHEEEEEEEEEECCCCCCEEEEECCCCCCEEECHHHHCCCCCCCHHHHH
DLLKTRVWEISRWRNKAAAAGVGIGGLKIVGNEDGNTGVPLKDGVMIPVSSIEKAPSAEL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHCCCCCCCC
RPGQKDSDSLPEYALLDKVLAAYIEHAHGRADLLADGFDQATVDTVMRLVDRAEWKRRQY
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
PLGPKVTALAFGRDRRLPVTNAFRE
CCCCCEEEEEECCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]